Who lives in our dishwasher? Preliminar results of fungal metagenomic analysis of household dishwashers | Kdo živi v našem pomivalnem stroju? Preliminarni rezultati metagenomske analize gliv v gospodinjskih pomivalnih strojih

Английский. In the last few years the advances in molecular biological methods, especially the development of next generation sequencing, have drastically changed and improved our view of microbial world. Progress in new molecular techniques enables us to overcome potential disadvantages of traditional microbiological techniques in fungal community identifications. It also enables us to evaluate the richness of fungal populations more efficiently and reliably. In the present study, we used the Ion Torrent PGM next generation sequencing platform to analyse fungi present in ordinary household dishwashers. The identification was based on massive parallel sequencing of the D2 LSU rRNA amplicon. The analysis revealed rich and diverse fungal communities present in our dishwashers. Interpretation of the results was based on previously published research by Zalar et al. (2011). The results of our study confirmed that the new technology in many ways surpasses classical methods used in fungal analysis by offering quicker, reliable, more sensitive and inexpensive high-throughput identification of microorganisms in entire communities.

словенский. Na področju metagenomike je napredek molekularno bioloških metod, predvsem razvoj naslednje generacije sekvenciranja, dramatično spremenil in razširil pogled na mikrobni svet. Napredek novih molekularnih tehnik nam omogoča premagovanje pomanjkljivosti tradicionalnih mikrobioloških tehnik, predvsem pri identifikacij populacij gliv. Z novim pristopom dobimo učinkovitejšo in zanesljivejšo ocenitev števila vrst gliv v določenih populacijah. V naši raziskavi smo uporabili tehnologijo naslednje generacije sekveniranja Ion Torrent za analizo prisotnosti gliv v gospodinjskih pomivalnih strojih. Identifikacija je temeljila na masivni paralelni določitvi nukleotidnega zaporedja podenote D2 LSU glivnega gena rRNA. S končno analizo smo potrdili bogate in raznolike skupnosti gliv v naših pomivalnih strojih, interpretacija rezultatov pa je temeljila na že objavljenih predhodnih raziskavah Zalarjeve in sod. (2011). Rezultati naše raziskave so potrdili, da nova tehnologija na mnogih področjih presega klasične mikrobiološke metode, ki se uporabljajo pri analizi skupnosti gliv in ponuja hitrejšo, zanesljivejšo, občutljivejšo ter cenejšo identifikacijo mikroorganizmov v skupnosti.