Effects of dietary pectin on protein digestion and metabolism in growing rats | Vpliv pektina na prebavo beljakovin in metabolizem pri rastočih podganah

Английский. In an attempt to clarify the relationships between the digestive and metabolic effects of additional feeding of citrus pectin, the intestinal contents, tissues weights, nitrogen excretion and retention were studied in laboratory rats. Twenty-four growing male Wistar rats (98.8 g ± 5.3 g of body weight) were fed ad libitum for 20 days with balanced diets containing casein as the source of protein. In the experimental diet 96 g of wheat starch was replaced by 80 g of citrus pectin and 16 g of vegetable oil. Apparent digestibility and apparent protein biological value were calculated, the weights of digestive tissues and digestive organ content weights were also determined. All tissues of different parts of the digestive tract were heavier in the pectin fed group, and small intestine and caecum were statistically significant different as compared to control group. The contents of the small intestine and caecum were significant heavier in the pectin group. Pectin significantly lowered dry matter intake and growth rate and significantly increased faecal excretion of nitrogen and significantly decreased urinary nitrogen excretion. The consequence of alteration in the nitrogen excretion route was significantly lower apparent protein digestibility and apparent net protein utilisation, but apparent protein biological value was unchanged.

словенский. Da bi poskusili razložiti povezavo in vpliv krmljenja pektina iz limonine lupine na prebavo in presnovo, smo izmerili maso vsebine prebavil, maso tkiv prebavil ter izločen in absorbiran dušik pri laboratorijskih podganah. Štiriindvajset rastočih laboratorijskih podgan moškega spola seva Wistar (s povprečno telesno maso 98,8 g ± 5,3 g) smo 20 dni krmili ad libitum z uravnoteženo krmo, ki je vsebovala kazein kot vir beljakovin. V poskusni krmi smo 96 g pšeničnega škroba zamenjali z 80 g pektina iz limonine lupine in 16 g mešanice rastlinskih olj. Izračunali smo navidezno prebavljivost beljakovin in navidezno biološko vrednost beljakovin ter določili maso posameznih tkiv in vsebine prebavil. V poskusni skupini, ki je imela v krmi pektin, smo izmerili večjo maso tkiv posameznih delov prebavil v primerjavi s kontrolno skupino, s statistično značilnimi razlikami pri tankem in slepem črevesju. Masa vsebine tankega in debelega črevesja je bila statistično značilno večja pri skupini s pektinom. V skupini s pektinom v krmi so živali zaužile značilno manj suhe snovi krme in imele manjše priraste, značilno več izločenega dušika v blatu, pa tudi značilno manj izločenega dušika preko seča. Posledica razlik v izločanju dušika preko blata ali seča je značilno manjša navidezna prebavljivost in navidezna neto izkoristljivost beljakovin, medtem, ko je navidezna biološka vrednost beljakovin ostala nespremenjena.