Zygote cryobanking applied to CRISPR/Cas9 microinjection in mice. (Research article).
2024
SCHLAPP, G. | MEIKLE, M. N. | PÓRFIDO, J. L. | MENCHACA, A. | CRISPO, M.
ABSTRACT.- Microinjection of CRISPR/Cas9 requires the availability of zygotes that implies animal breeding, superovulation schemes, and embryo collection. Vitrification of zygotes may allow having ready-to-use embryos and to temporally dissociate the workload of embryo production from microinjection. In this study, fresh (F group) or vitrified (V group) zygotes were microinjected with CRISPR/Cas9 system to test the hypothesis that vitrified zygotes could be a suitable source of embryos for microinjection. @ Schlapp et al.
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