Evaluation of Peroxyacetic Acid and Chlorine as Treatments for Surface Water for Post-Harvest Uses in the Produce Industry
2022
Zilfa Irakoze | Londa Nwadike | Don Stoeckel | Manreet Bhullar | Patrick Byers | Sara E. Gragg
Nearly half of foodborne illnesses are linked to produce and nuts, and water used for produce post-harvest activities can contribute to contamination. Surface water serves as an economical source for agricultural activities: however, exposure to the environment increases microbial risks and impacts its physicochemical characteristics. In this study, peroxyacetic acid (PAA) and chlorine (Cl) were evaluated as treatments for simulated surface water to determine their efficacy at achieving &lsquo:no detectable generic Escherichia coli&rsquo: in 100 mL. Simulated surface water was prepared to turbidities of 2 and 100 NTU, adjusted to pH 6.5 or 8.4, equilibrated to 32 or 12 °:C, inoculated with 5 logs per mL of non-pathogenic (generic) E. coli, and treated with Cl 25 ±: 2 ppm, PAA 75 ±: 5 ppm, or sterile water control (W). Dey-Engley neutralization was followed by enumeration on E. coli/Coliform Petrifilm at times (t) 0 to 2880 min (48 h) post-treatment. When not detected, treatments were further evaluated through enrichment in 2X Brain Heart Infusion (BHI) broth. Enrichments were streaked on MacConkey agar (MAC) to confirm E. coli absence. All Cl and PAA treated samples were below the test limit of detection (<:5 CFU/mL), and E. coli was not detected in 5 mL enrichments even at t = 0 (shortly after treatment). These data suggest that Cl and PAA interventions may be effective for treating surface water for post-harvest uses.
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