Effects of Glutamine on Rumen Digestive Enzymes and the Barrier Function of the Ruminal Epithelium in Hu Lambs Fed a High-Concentrate Finishing Diet
2022
Qiujue Wu | Zhongying Xing | Jiahui Liao | Longlong Zhu | Rongkai Zhang | Saiqiao Wang | Cong Wang | Yan Ma | Yuqin Wang
The present experiment aimed to research the effects of glutamine (Gln) on the digestive and barrier function of the ruminal epithelium in Hu lambs fed a high-concentrate finishing diet containing some soybean meal and cottonseed meal. Thirty healthy 3-month-old male Hu lambs were randomly divided into three treatments. Lambs were fed a high-concentrate diet and supplemented with 0, 0.5, and 1% Gln on diet for 60 days. The experimental results show that the Gln treatment group had lower pepsin and cellulase enzyme activity, propionate acid concentration, and IL-6, TNF-&alpha:, claudin-1, and ZO-1 mRNA expression in the ruminal epithelium (p <: 0.05): as well as increases in lipase enzyme activity, the ratio of propionic acid to acetic acid, the IL-10 content in the plasma, and the mRNA expression of IL-2 and IL-10 in the ruminal epithelium, in contrast to the CON (control group) treatment (p <: 0.05). Taken together, the findings of this present study support the addition of Gln to improve digestive enzyme activity, the ruminal epithelium&rsquo:s barrier, and fermentation and immune function by supplying energy to the mononuclear cells, improving the ruminal epithelium&rsquo:s morphology and integrity, and mediating the mRNA expression of tight junction proteins (TJs) and cytokines.
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