Differences in Physiological Characteristics of Green Prickly Ash Germplasm Resources in Response to Low-Temperature Stress

In this study, we investigated the physiological response to low-temperature stress and comprehensively evaluated the cold resistance of green prickly ash germplasms. One-year-old branches of 23 green prickly ash varieties were used as experimental materials, three branches were selected from each variety, and three replicates of single branches were established. Subsequently, the physiological responses of the 23 green prickly ash germplasm resources to low-temperature stress were analyzed based on the relative conductivity (REC), osmotic adjustment substances, and antioxidant enzyme activities. We found that as the temperature decreased, the REC of each germplasm increased. The most rapid REC increase occurred from 0 to &minus:10 &deg:C and continued to gradually gently increase from &minus:10 to &minus:30 &deg:C. The lethal dose-50 temperature (LT50) of the germplasms was between 3.16 and &minus:12.37 &deg:C. The top three cold-resistant varieties were TJ, SCHJ, and CJ, and the last three cold-resistant varieties were EWJ, DYSJ, and HYXJ. The results of a correlation analysis revealed a significant correlation between superoxide dismutase (SOD) activity and REC, between REC and free protein, and between SOD activity and catalase. The results of the PCA revealed that the first category includes 5 pepper varieties of CJ, LFJ, and XYTJ with higher cold-resistance score indexes: the second category includes 13 pepper varieties of YQ2 H, WCFZ, and TZJ with appropriate scores: and the third category includes 5 pepper varieties of HYXJ, HNJ, and DYSJ with lower scores. Cluster analysis was performed to classify the cold-resistance physiological indices, and four groups were obtained. The most cold-resistant groups included CJ and LFHJ, whereas the most cold-sensitive groups included HYXJ, DYSJ, and HNJ. Finality, the subordinate function method was used to determine the cold resistance from strong to weak. The varieties with the strongest cold resistance were LFJ, EWJ, and CJ, and the weakest varieties were DYSJ, YQ1H, and HYXJ. The strongest cold-resistant varieties were LFHJ and CJ, followed by DYSJ, YQ1H, and HYXJ. Based on multiple lines of evidence, the most cold-resistant varieties were CJ and LFJ, while the most cold-sensitive varieties were DYSJ and HYXJ. In conclusion, in this study, we elucidated the low-temperature tolerance of different varieties of green prickly ash, and varieties with high cold resistance were selected. These findings provide theoretical guidance and technical support for the screening of cold-resistant green prickly ash germplasms, which will facilitate their introduction to northern China.