Inhibitory Effects of Aqueous and Hydroalcoholic Extracts from Jatobá Coat (Hymenaea courbaril L.) on Pancreatic Amylase and Starch Absorption
2025
Ana Caroline Polo | Thaís Marques Uber | Gustavo Henrique Souza | Rúbia Carvalho Gomes Corrêa | José Rivaldo dos Santos Filho | Anacharis Babeto de Sá-Nakanishi | Flávio Augusto Vicente Seixas | Adelar Bracht | Rosane Marina Peralta
Jatobá: (Hymenaea courbaril) is a native tree abundant in Brazil. The fruit coat is an industrial by-product of jatobá: flour processing, typically discarded. Presently, within the circular bioeconomy concept, there are efforts underway that aim at finding economically viable applications for the bio-residues of jatobá:. Within this context, the present work attempts to find possible applications for the jatobá: coat in glycemic control through inhibition of &alpha:-amylase activity. Aqueous and hydroethanolic extracts were used. In vitro experiments included detailed kinetic studies with an &alpha:-amylase catalyzed reaction. Starch absorption in vivo was assessed by means of a starch tolerance test in mice. Both extracts inhibited &alpha:-amylase. The IC50 values for the aqueous and hydroalcoholic extracts were 81.98 ±: 3.53 µ:g/mL and 51.06 ±: 0.42 µ:g/mL, respectively. The inhibition was of the non-competitive type. Both extracts reduced hyperglycemia caused by starch administration in mice, the aqueous extract being effective over a larger dose range. This action can be attributed to the &alpha:-amylase inhibition. In silico studies suggested that procyanidin dimers, taxifolin 7-O-rhamnoside, and quercetin 7-rhamnoside contribute, but several other not-yet-identified substances may be involved. The findings suggest that aqueous and hydroalcoholic extracts from jatobá: coat warrant further investigations as potential modulators of glycemia following starch ingestion.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Multidisciplinary Digital Publishing Institute