A Novel Transcription Factor of Regulating Ag-8 Biocontrol to Grapevine Crown Gall
2025
Shiyu Li | Yaping Zhang | Zhenxing Liu | Yilin Gu | Yue Bi | Jianyu Yang | Weiwei Yu | Zhuoran Li | Yuanhong Wang
Grapevine crown gall (GCG) is a major disease caused by the Allorhizobium vitis that causes significant losses in the grape industry. In this study, a strain of Agrobacterium tumefaciens (Ag-8) from soil was found to have a significant effect on grapevine crown gall. The present study was conducted to investigate the potential impact of the AraC family transcription factor ata (Agrobacterium :tumefaciens AraC) on the biocontrol capacity of Ag-8 and to elucidate the potential mechanisms of its action. To this end, an ata deletion mutant (&Delta:ata) was constructed. It was found that the biofilm, motility, and colonization of the ata deletion mutant strain were significantly lower than those of the wild strain Ag-8. By analyzing the &Delta:ata transcriptome data, we focused our attention on the ABC transporter protein araH, and RT-qPCR showed that it was significantly down-regulated in the &Delta:ata strain. In a follow-up study, the candidate gene araH deletion strain (&Delta:araH) was found to have significantly lower biofilm formation, motility, and biocontrol ability than the wild-type strain Ag-8. Therefore, araH may be implicated as a pivotal target gene of ata in modulating the biocontrol capability of Ag-8. This study supports developing biocontrol strategies targeting ata-regulated colonization in Agrobacterium to enhance the sustainable management of grapevine crown gall.
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