Biological Control of Black Spot Disease in Cherry Tomato Caused by Alternaria alternata with Bacillus velezensis T3
2025
Xinmeng Wei | Qiya Yang | Dhanasekaran Solairaj | Esa Abiso Godana | Xi Zhang | Yu Li | Xiaoyong Liu | Hongyin Zhang
Black spot is a major postharvest disease of cherry tomatoes, caused by Alternaria alternata. This causes economic losses and storage challenges, so researchers are exploring alternative methods. The biological control of fruits and vegetables using antagonistic bacteria and yeasts is currently a research hotspot. Initially, the biological control impact of Bacillus velezensis T3 on cherry tomato black spot was investigated. Disease defense, scavenging reactive oxygen species, and antioxidant-related enzymes were determined during different storage periods. The relative gene expressions of these enzymes were also confirmed using RT-qPCR. The results showed that B. velezensis T3 reduced the incidence of black spot disease in cherry tomatoes. The growth of A. alternata was suppressed by B. velezensis T3 cell-free filtrate both in vitro and in vivo. In addition, B. velezensis T3 induced the activities of disease resistance-related enzymes such as polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), &beta:-1,3-glucanase (GLU), and chitinase (CHI), and the activities of the ROS-related enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), and reduced the rate of O2&minus: production and H2O2, and MDA content of cherry tomatoes. This approach offers a promising alternative for extending shelf life, though further studies are needed to fully characterize its effects on fruit quality.
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