The Combined Application of Biological Nanoselenium and Biochar Promotes Selenium Enrichment and Cadmium Content Reduction in Rice

Cadmium (Cd) pollution in rice and selenium (Se) deficiency in humans have attracted widespread attention. In this study, we investigated the effects of the combined application of biological nanoselenium (B-SeNPs) foliar spray and biochar (BC) on Se enrichment and Cd content reduction in rice. A pot experiment was established by designing four levels each of BC and B-SeNPs to be applied to rice plants. The results revealed that soil Cd bioavailability decreased by 3.26&ndash:16.67%, while soil Se bioavailability increased by 0.76&ndash:7.63% in the combined BC and B-SeNPs treatments, with rice photosynthesis showing significant enhancement during each growth period. Both BC and B-SeNPs treatments significantly enhanced the levels of antioxidant components (glutathione, phytochelatins, catalase, peroxidase, and superoxide dismutase) while reducing oxidative stress markers (malondialdehyde and superoxide anion radical) in rice leaves. Additionally, these treatments effectively modulated the subcellular distribution of Se and Cd, demonstrating their potential in alleviating Cd toxicity and enhancing Se homeostasis. These changes were accompanied by a marked reduction in lipid peroxidation (indicated by malondialdehyde) and superoxide radical accumulation, suggesting that BC and B-SeNPs treatments strengthened the antioxidative defense system in rice leaves. Additionally, compared with the BC0Se0 treatment, the combined application of BC and B-SeNPs significantly enhanced grain Se content by 7.14&ndash:221.43% while significantly reducing Cd content by 30.77&ndash:76.92%. The efficacy of grain Se enrichment and Cd reduction followed the sequence B-SeNPs + BC &gt: Se only &gt: BC only, where the BC5Se20 treatment demonstrated the most pronounced effects on both Se accumulation and Cd decrease in grains. Therefore, the combined application of foliar-applied B-SeNPs and biochar not only reduces Cd bioavailability in soil but also effectively suppresses Cd uptake by rice while simultaneously enhancing Se enrichment.