Neutrophils from tuberculosis patients are polarized toward pro-inflammatory and anti-inflammatory phenotypes according to the disease severity

Neutrophils are the first line of defense against pathogens, combating them by using several antimicrobial mechanisms. These cells display a remarkable plasticity that can be molded by the different environments that neutrophils confront to protect the host, therefore presenting diverse phenotypes. Actually, pro- and anti-inflammatory neutrophils populations (N1- and N2-like phenotypes) have been described in cancer and inflammatory disorders. However, the identification of N1/N2 neutrophil subtypes in human intracellular bacterial diseases remains unexplored. Here, we characterized neutrophils from tuberculosis (TB) patients presenting distinct immunological status according to their disease severity. TB patients were classified as high or low responders (HR or LR) in accordance with their immunity against Mycobacterium tuberculosis (Mtb). Interestingly, by analyzing the phenotypic and functional characteristics of neutrophils from the two groups of TB patients we demonstrated that HR patient’s neutrophils display a pro-inflammatory N1-like phenotype, whereas LR patient’s neutrophils show an anti-inflammatory N2-like phenotype. Remarkably, whereas neutrophils from both groups of patients phagocytized MtbH37Rv strain equally, HR TB’s neutrophils displayed a significantly increased ability to kill pathogenic Mtb as compared to neutrophils from LR TB patients that presented a diminished capacity of bacterial elimination. Together, our findings suggest the existence of different subtypes of neutrophils in TB patients according to their immune response to Mtb and disease severity, indicating that neutrophils might be promising targets for TB host-directed therapy.

Instituto de Biotecnología

Fil: Morelli, María Paula. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina

Fil: Martin, Candela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina

Fil: Martin, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Pellegrini, Joaquín Miguel. Parc Scientifique et Technologique de Luminy. Aix-Marseille Université. Centre National de la Recherche Scientifique (CNRS). Centre d'Immunologie de Marseille Luminy; Francia

Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina

Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina

Fil: Bigi, Fabiana. Consejo Nacional de investigaciones Científicas y Técnicas; Argentina

Fil: Ciallella, Lorena. Hospital F. J. Muñiz. División Tisioneumonología; Argentina

Fil: Musella, Rosa María. Hospital F. J. Muñiz. División Tisioneumonología; Argentina

Fil: Rodriguez Mieres, Adriana. Hospital F. J. Muñiz. División Tisioneumonología; Argentina

Fil: de Casado, Graciela C. Hospital F. J. Muñiz. División Tisioneumonología; Argentina

Fil: Palmero, Domingo. Hospital F. J. Muñiz. División Tisioneumonología; Argentina

Fil: García, Verónica Edith. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina

Fil: García, Verónica Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina