Multiplication of Axillary Shoots of Adult Quercus robur L. Trees in RITA® Bioreactors
2025
Paweł Chmielarz | Conchi Sánchez | João Paulo Rodrigues Martins | Juan Manuel Ley-López | Purificación Covelo | María José Cernadas | Anxela Aldrey | Saleta Rico | Jesús María Vielba | Bruce Christie | Nieves Vidal
Adult trees of pedunculate oak (Quercus robur L.) are recalcitrant to vegetative propagation. In this study, we investigated the micropropagation of five oak genotypes corresponding to trees aged 60&ndash:800 years in a liquid medium. We used commercial RITA bioreactors to study the influence of the explant type, the culture medium, shoot support and number of immersions. Variables evaluated included the number of normal and hyperhydric shoots, shoot length, multiplication coefficient and number of rootable shoots per explant. All genotypes could be cultured in temporary immersion. Basal stem sections attached to callus grew better than apical sections and developed less hyperhydricity. For long-term cultivation, Gresshoff and Doy medium was the best of the three media evaluated. All genotypes produced vigorous shoots suitable for rooting and acclimation. This is the first protocol to proliferate adult oak trees in bioreactors, representing significant progress towards large-scale propagation of this and other related species.
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