Optimizing Effects of Organic Farming and Moderately Low Nitrogen Levels on Soil Carbon and Nitrogen Pools

Reasonable nitrogen fertilizer management and cultivation methods can enhance the nitrogen supply and carbon sequestration capabilities of soil, which is beneficial for meeting the growth requirements of crops and alleviating environmental issues. However, the existing research on optimizing nitrogen use efficiency and soil carbon sequestration in organic systems remains limited. Therefore, a field trial was conducted to elucidate the impacts of different cultivation patterns and nitrogen application rates on soil carbon and nitrogen pools, especially on how these factors affect the components of soil organic carbon. The treatments included conventional cultivation with low nitrogen treatment (CFN12), conventional cultivation with high nitrogen treatment (CFN18), organic cultivation with low nitrogen treatment (OFN12), and organic cultivation with high nitrogen treatment (OFN18). The results demonstrated that, relative to CFN18, OFN12 significantly increased the accumulation amounts of organic carbon and nitrogen in paddy soil. This was evident under multiple classifications of organic carbon, while it showed no advantage in the accumulation of mineral nitrogen. Notably, the organic cultivation mode increased the activities of enzymes involved in the carbon–nitrogen cycle in the cultivated layer and optimized the structure of humus, which gave the proportion of aggregates with a particle size greater than 0.5 mm more advantages. Correlation analysis demonstrated that the pertinent indices associated with soil carbon and nitrogen pools exhibited a highly significant positive correlation in the topsoil layer, accompanied by pronounced synergistic interactions among them. The PCA comprehensive scoring results indicate that OFN12 has the highest total score, indicating that it is beneficial for the improvement of soil fertility. This study offers practical insights for improving soil health, boosting plant growth, and enhancing climate mitigation through soil carbon storage, contributing to more sustainable agricultural practices.