Qualitative changes in the proteins of cotyledons during cold and warm stratification of Acer platanoides seeds
Tomasz Malinowski | Zofia Szczotka
In Norway maple (Acer platanoides L.) seeds, during dormancy breakage there appeared some qualitative changes in protein composition of the cotyledons. After 48h of soaking and in the first week of stratification, the number of different proteins decreased. This decrease in number of proteins was probably associated with the effect of hydration of embryo tissue. Protein patterns from imbibed seeds exposed to cold (3oC) and warm (15oC) treatments were not significantly different. The influence of temperature on protein patterns became visible in the third week of stratification, when a few new proteins appeared. One of them, designated B, appeared only during cold stratification before radicles protrusion. They were also present in seeds that had completed germination. These proteins are presumably associated with the process of dormancy breakage.
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