Lignocellulose Degradation and Enzymatic Hydrolysis of Soybean Incorporated Sorghum Silage Inoculated with Feruloyl-Esterase Producing <i>Lactobacillus plantarum</i>
Samaila Usman | Fuhou Li | Dong An | Na Shou | Jianqiang Deng | Yixin Zhang | Xusheng Guo | Yuying Shen
Availability of feedstock is one of the uncertainties impeding cellulosic biofuel production, and conservation of whole crop biomass as silage is a promising method to ensure year-round feedstock availability for biofuel production. This study investigated lignocellulose degradation and subsequent enzymatic hydrolysis of a 90-d sorghum silage incorporated with soybean and inoculated with <i>Lactobacillus plantarum</i> A1 in a three (0, 25, and 50%; soybean inclusions) by two (uninoculated and inoculated) factorial experiment. The results revealed that <i>L. plantarum</i> A1 inoculated silages had improved fermentation characteristics. The silages’ total N and non-fiber carbohydrate increased with <i>L. plantarum</i> A1 and soybean inclusion (<i>p</i> < 0.05). Inoculation also increased the residual water-soluble carbohydrate by 33.9% (<i>p</i> < 0.05). Inoculation and soybean inclusion significantly hydrolyzed the biomass’ lignocellulose, altered its morphology and microstructural matrix, increased production of ferulic acid and reduced the biomass crystallinity by 15.60% (<i>p</i> < 0.05). <i>L. plantarum</i> A1 inoculation × soybean inclusion improved glucose yield and cellulose conversion during enzymatic saccharification compared to uninoculated treatments. Therefore, incorporating soybean into sorghum silage with <i>L. plantarum</i> A1 inoculation enhanced fermentation quality, lignocellulose degradation and enzymatic saccharification which could serve as a sure way for sustainable year-round feedstock supply for enhanced biofuel production.
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