Optimization of the Culture Medium of <i>Beauveria bassiana</i> and Spore Yield Using Response Surface Methodology
Hanxiao Lou | Qizheng Luo | Qingqing Guo | Runshen Su | Jianfeng Liu | Hongli He | Yunqing Cheng
<i>Beauveria bassiana</i> has distinct nutrient requirements depending on the growth stage, with the mycelial growth stage being particularly nutrient-intense. When nutrients are exhausted, <i>B. bassiana</i> produces numerous spores that re-germinate and release visible hyphae under suitable environmental conditions. This study aimed to use low-cost agricultural by-products for developing an optimized sporulation medium. Single-factor experiments revealed that potato, wheat bran, and wheat had the greatest influence on sporulation after 14 d of culture. Wheat, wheat bran, and corncob had the strongest effect on spore production. Using a mathematical model and Box–Behnken design, the maximum predicted sporulation using the stock medium was 5.55 × 10<sup>6</sup>/mL. The optimized formula comprised 54.82 g potato, 77.74 g wheat bran, and 101.48 g wheat. The actual stock medium produced a spore yield of 5.52 × 10<sup>6</sup>/mL. Similarly, the maximum predicted sporulation was 15.66 × 10<sup>6</sup>/mL for the culture medium. The optimized formula comprised 50.81 g wheat, 99.6 g wheat bran, and 112.59 g corncob, with a spore yield of 15.69 × 10<sup>6</sup>/mL. The high consistency between predicted and experimental values indicated that our model can accurately determine the effect of medium on sporulation.
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