Continuous Bioproduction of Alginate Bacterial under Nitrogen Fixation and Nonfixation Conditions
Pablo Contreras-Abara | Tania Castillo | Belén Ponce | Viviana Urtuvia | Carlos Peña | Alvaro Díaz-Barrera
Alginate is a biomaterial produced by <i>Azotobacter vinelandii</i>, a diazotroph that, under nitrogen-fixing conditions, can fix nitrogen under high oxygen levels. In <i>A. vinelandii,</i> alginate is synthesized from fructose-6P via synthesis of precursor, polymerization, and modification/exportation. Due to its viscosifying, gelling, and thickening characteristics, alginate is widely used in food, pharmaceutical, and cosmetical industries. This study aimed to develop a continuous bioprocess and a comparative analysis of alginate production under diazotrophic and nondiazotrophic conditions. Continuous cultures were developed at three dilution rates (0.06, 0.08 and 0.10 h<sup>−1</sup>). In steady state, the respiratory activity, alginate production, alginate molecular weight and the genes encoding alginate polymerase were determined. Under the conditions studied, the specific oxygen uptake rate and respiratory quotient were similar. The diazotrophic conditions improved the conversion of sucrose to alginate and the specific productivity rate, which was 0.24 ± 0.03 g g<sup>−1</sup> h<sup>−1</sup>. A higher alginate molecular weight (725 ± 20 kDa) was also achieved under diazotrophic conditions, which can be explained by an increase in the gene expression of genes <i>alg8</i> and <i>alg44</i> (encoding polymerase). The results of this work show the feasibility of enhancing alginate production (yields and specific productivity rates) and quality (molecular weight) under nitrogen-fixing conditions, opening the possibility of developing a continuous bioprocess to produce alginate with specific characteristics under conditions of diazotrophy.
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