Development and effect of a Lactobacillus plantarum inoculant on quality of maize grain silage | Razvoj i uticaj Lactobacillus plantarum inokulanta na kvalitet silaže od zrna kukuruza

The main aim of these studies was the characterisation and identification of lactic acid (LAB) bacteria isolated from untreated silage, and the effect of selected bacteria (inoculant was called Silko for maize) on ensiling of maize high-moisture grain. Four isolates of LAB (L1, L2, L3 and L4) were characterised by the use of phenotypic assays and identified by phylogenetic analysis of 16S rRNA as L. plantarum. The fresh maize high-moisture grain was ensiled with a Silko for maize inoculant, inoculant available in the market (positive control) and no additive (untreated; negative control). After 60 days of ensiling, the results showed that the chemical composition and fermentation characteristics were better in treated silages with inoculants compared to the negative control. The contents of ash, fat and lactic acid (LA) were significantly higher in the silages treated with inoculants than in negative control. In comparison, the contents of cellulose, acid detergent fibre (ADF), neutral detergent fibre (NDF), NH3-N/total nitrogen and butyric acids (BA) were considerably lower in silage treated with Silko for maize compared to the positive control. The Silko for maize improve nutritional value and fermentation of maize grain silage and is a competitive product on the market.

Cilj ovih istraživanja bio je karakterizacija i identifikacija bakterija mlečne kiseline (BMK) izolovanih iz netretirane silaže, kao i efekat odabranih bakterija (inokulant nazvan Silko za kukuruz) na siliranje vlažnog zrna kukuruza. Četiri izolata BMK (L1, L2, L3 i L4) su okarakterisani upotrebom fenotipskih testova i identifikovani filogenetskom analizom 16S rRNA kao L. plantarum. Vlažno zrno kukuruza silirano je sa Silkom za kukuruz, inokulantom koji je dostupan na tržištu (pozitivna kontrola) i bez primene inokulanta (netretirana; negativna kontrola). Nakon 60 dana od siliranja, rezultati su pokazali da su hemijski sastav i fermentacione karakteristike bolji u silažama tretiranim sa inokulantima u poređenju sa negativnom kontrolom. Sadržaj pepela, masti i mlečne kiseline bio je značajno veći u silažama tretiranim sa inokulantima nego u negativnoj kontroli. Sadržaj celuloze, kiselih (ADF) i neutralnih deterdžentskih vlakana (NDF), amonijačnog azota u ukupnom azotu i buterne kiseline (BA) bio je značajno niži u silaži tretiranoj sa Silkom za kukuruz nego u pozitivnoj kontroli. Silko za kukuruz poboljšava hranjivu vrednost i fermentaciju silaže od zrna kukuruza i predstavlja konkurentan proizvod na tržištu.