Difference in Phytochemical Composition and Antioxidant Activity Screening of Two Methanolic Sage Extracts
2024
Janić, M. | Krstić, A. | Valenta Šobot, A. | Momić, T. | Brković, S. | Tošić, M. | Filipović Tričković, J.
Sage has been used in traditional medicine to prevent and treat many health problems. This study aimed to determine and compare the phytochemical composition and antioxidative activity of sage leaf extracts obtained with 50% methanol (50MSE) and 80% methanol (80MSE). We determined the total phenolic content (TPC), total tannin content (TTC), and total flavonoid content (TFC) in extracts. Terpenoids were identified using gas chromatography-mass spectrometry (GC–MS), and the radical scavenging capacity of extracts was measured using DPPH assay. TPC was higher in 80MSE, TTC was higher in 50MSE, and TFC did not differ. GC–MS analysis showed that the content of α thujone and L-camphor was low in both extracts, with higher content in 50MSE. DPPH assay indicated that a slightly lower IC50 value and higher antioxidant potential had 80MSE. Results pointed out that the polarity of the solvent and water content had different effects on the extraction of polyphenols and terpenoids.
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Издатель Vinča Institute of Nuclear Sciences – National Institute of the Republic of Serbia, University of Belgrade
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