Molecular Identification of Bacteria Involved in Degradation of Crude Oil
Nwakanma, C. | Obih, E.C. | Onyia, O.
In this present study, bacteria were isolated from soil obtained from oil contaminatedmechanic site in Enugu. Out of the 206 forming colonies, 16 colonies were selected basedon observable morphological differences and screened for crude oil degraders. Out of the16 isolates, 11 isolates were assumed to be degraders because they formed maximumclear zones on mineral salt media. At the end of 14 days incubation, 14 out of the 16isolates degraded the crude oil ranging from 5% to 60%. Isolate 1, 5 and 14 had thehighest percentage degradation of 60%, followed by isolate 13 with 50% degradation.Isolate 3 was the least degrader with 5% degradation while isolate 2 and 16 were notable to degrade the crude oil. An attempt to identify the isolates was done by sequencingthe 16s rRNA gene of the isolates using DNA sequencing technique. Isolate 2, 3, 4, 5, 6,and 7 were identified as Escherichia coli, Streptococcu anginosus strain SK52,Lactobacillus spp. G12, Bacillus cereus strain ABc17, Paracoccus spp. KF89 andLactobacillus spp. G22 respectively. However, isolate 1, 8, 9, 10, 11, 12, 13, 14, 15 and 16 did not produce any result. This study shows that microorganisms isolated from a nonnative crude oil contaminated site can be utilized for bioremediation.Key words: Bioremediation, 16s rRNA gene, DNA sequencing, Biotechnology
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