Evaluation of the presence of arabinogalactan proteins and pectins during Quercus suber male gametogenesis
2015
Costa, Mário Luís | Sobral, Rómulo Sacramento | Costa, Maria Manuela Ribeiro | Amorim, Maria Isabel | Coimbra, Sílvia
Supplementary data are available online at www.aob.oxford journals.org and consist of the following. Figure S1: Quercus suber predicted AG peptide sequences. Table S1: Gene-specific primers used in qRT-PCR analysis.
Показать больше [+] Меньше [-]Quercus suber (cork oak) is a dominant tree of the Fagaceae in forests of the south-west Iberian Peninsula. It is monoecious with a long progamic phase that provides a comprehensive system for comparative studies in development and sexual reproduction. In this study the distribution of arabinogalactan protein (AGPs) and pectin epitopes in anthers of Q. suber was assessed to map these hydroxyproline-rich glycoproteins and the galacturonate-rich acidic polysaccharides during pollen development. Methods Immunolocalization in male flowers was performed with a set of monoclonal antibodies directed against the carbohydrate moiety that recognizes AGPs and pectins. To identify AGP genes involved in cork oak male flower development, a search was conducted for annotated AGP genes in the available transcriptome data of the Cork Oak EST Consortium database (www.corkoakdb.org).
Показать больше [+] Меньше [-]This work was funded by FEDER funds through the Operational Competitiveness Programme – COMPETE and by National Funds through FCT – Fundac¸a˜o para a Cieˆncia e a Tecnologia under the project FCOMP-01-0124-FEDER019461 (PTDC/AGR-GPL/118508/2010). R.S. was supported by funding from FCT with a PhD grant (ref. SFRH/BD/84365/ 2012).
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