Macrophage Activation and Intracellular Signaling Pathway by the Polysaccharide Isolated from Broccoli
2024
Park, J.H. | Shin, K.S.
This study examined the macrophage-stimulating effects and signaling mechanisms of immune-active polysaccharide (BCE-0) prepared from broccoli. BCE-0 is a typical pectic polysaccharide composed of neutral sugars (57.7%), such as galactose (19.2%), rhamnose (14.4%), arabinose (12.2%), and uronic acid (34.9%). BCE-0 enhanced cell proliferation and increased the secretion of cytokines and chemokines, including interleukin (IL)-6, tumor necrosis factor-α, and nitric oxide (NO), in a dose-dependent manner. BCE-0 induced the phosphorylation of mitogen activated protein kinase (MAPK)- and NF-κB-related proteins, such as p38. JNK, ERK, IκBα, p65, and c-Jun. In specific inhibitor experiments, the effects of BCE-0 on IL-6 secretion were mostly promoted by NF-κB. Furthermore, different inhibitors showed that NO secretion was mostly induced via p38, JNK, and NF-κB. These findings suggest that BCE-0 exerts potent macrophage-activating effects and activates the MAPK and NF-κB pathways in macrophages, leading to the secretion of IL-6 and NO.
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