Relevance. Tomato is the most widespread vegetable crop in the southern regions of Russia and one of the most popular with the population in all natural and climatic zones of the country. The majority of products is produced in large vegetable-growing, farming and private household farms. One of the most important tasks is to improve the assortment taking into account natural and climatic conditions of cultivation and directions of use. In this regard, the selection of industrial tomato with a high level of flexibility and resistance to biotic and abiotic stressors for the south of Russia is an urgent task.   The aim of the study is to evaluate economically valuable features of perspective lines and new hybrids of tomato of industrial type, adapted to the conditions of southern Russia.   Material and methodology. The work was carried out in the open field conditions of experimental production farm of the Federal Scientific Center of Vegetable Industry (Moscow region) and Biryuchekutskaya vegetable breeding experimental station – branch of the FSBSI FSVC (Rostov region).   The objects of research were breeding material and 6 perspective new hybrid combinations of tomato of selection of the Federal State Budgetary Scientific and Scientific Institution of the Russian Federation.   Results and discussion. As a result of step-by-step study and selection, the most promising samples were identified for further use as source material for practical breeding: B-6-C-2014, VS-B-№7, VS-B-18, VS-B-20, VS-B-35, VS-B-38. As a result of the competitive testing, the best hybrid combinations were identified: F1 B-1, F1 B-2, F1 B-4, meeting the requirements of commercial production in the south of Russia. It is shown that the hybrid combination F1 B-2 exceeds both standard samples F1 Meteor and F1 Perfectpeel both in terms of total yield (by 10.9 t/ha and 9.6 t/ha), marketability of fruits, and dry matter content in fruits (6.1 % vs. 5.1% and 5.2 %). Taking into account the complex of economic characteristics, a precocious F1 hybrid of tomato F1 B-2 called F1 Profy has been submitted to the State Variety Testing, as it shows stable economic characteristics over several years of study.

Relevance. ICarrots are known all over the world. It contains a large amount of carotene-provitamin A and a lot of useful substances – vitamin C, sugar pectin’s, potassium, and essential oils. This valuable crop is grown all over the world and in different weather and climatic conditions. When growing carrots, it is necessary to carefully select varieties and hybrids that will give a high commercial yield, as well as are resistant to biotic and abiotic factors.Materials and methods. The research was conducted in 2018-2019 in the fields of the Biryuchekut vegetable breeding experimental station of the branch of the Federal Scientific Vegetable Center in open ground conditions. The object of research was the varieties of carrots.Results. While breeding work on the Biryuchekutsky vegetable breeding experimental station a new variety of carrot Aksinya was obtained. In 2018, it was included in the station variety test. The new promising variety has an earlier maturation period on average 10 days earlier than the standards, a conical, blunt-edged form of orange-colored root crops with high taste qualities. The yield is at the level of 45 t / ha. It has excellent preservation during winter storage, resistance to diseases, as well as the ability to grow in arid and hot climates on heavy soils, while maintaining a high marketability of 72-76%.

Research was carried out on the basis of the laboratory of breeding and seed production of legumes Federal state budgetary scientific institution "Federal scientific center of vegetable growing" (Moscow region) in 2013-2017. The study included 22 varieties of vegetable pea, differing in the length of the growing season and a weigt of 1000 seeds. Sowing was carried out in the optimal time, repeat the experience fourfold. Seeding rate 100-110 seeds per 1 m2. The selection of seeds four times on each plot. The sign «weight of 1000 seeds» determines the grain size and is the most important in the production of canned «green peas». With the increase in grain size decreases marketability of products, increases the consumption of seeds for sowing, significantly reduces the multiplication factor, which leads to higher prices of products. Therefore, varieties with a weigt of 1000 seeds 200-220 grams are mainly used for freezing. Industry currently uses varieties characterized by an average grain size (7-10mm) and a weight of 1000 seeds less than 200 grams, promising grades with a mass of 100-140 grams. To accelerate the selection process for creating varieties with specified parameters, a detailed analysis of the characteristics of the productivity of the initial material, in particular, the weight of 1000 seeds, is needed, since samples with an average size (160-200 grams) have a low coefficient of phenotypic variability and depend on the interaction of genotype and environment, but the reaction of each genotype to the environment is specific. Work should include samples with the lowest coefficient of variability. When working with finely seeded specimens, the average level of phenotypic variability should be taken into account and, when included in the breeding process, increase the selection sample.

Relevance. Hybrid seed production of carrots is carried out on a three-line basis, which suggests the presence of a male sterile maternal line and a fertile paternal line. For the reproduction of a male sterile line, a maintainer line is needed. The process of creating an isogenic pair of a manly sterile line – a maintainer line is of great complexity. As a maintainer line, it is necessary to use only a plant – a homozygote Cyt N msms for the core genes providing sterility, but with a normal cytoplasm. The purpose of the work is the creation of maintainer line for the sterility of carrots from the population of the Rogneda variety.Methods. Research were carried out: in 2016, at the experimental base of the Voronezh Vegetable Experimental Station (Voronezh Region); in 2017 - 2019, at the breeding center of All-Russian Research Institute of Vegetable Growing – branch of Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (Moscow Region). The material for the work was seed plants and roots of the Rogneda variety and hybrid, inbred, backcross progenies obtained from this population. Breeding methods: hybridization, inbreeding. Crosses used: simple direct and complex return (backcrosses), inbreeding.Results.Research revealed the possibility of searching for a maintainer line in the Rogneda variety. The P43f inbred progeny is potential line B, which maintains the CMS trait of the petaloid type at 100%. Of the 67 hybrid progenies, only P43st x P43f was left, in which all plants with male sterility. 65 hybrid progenies contained both sterile and fertile plants. Associated 65 inbred progenies had sterile and fertile plants, i.e. it makes no sense to try to obtain a maintainer line is needed. The process of creating an isogenic pair of a manly sterile line – a maintainer line from such fertile plants. Hybrid progeny of P4st x P4f had 100% fertile plants. The inbred progeny of P4f was represented only by fertile plants.

A promising direction in the elite seed growing flower seeds is to  create a  population with the given ratios decorative signs and, above all, painting flowers (racemes),  which has a  wide  range of  variability.   Maintaining constant these crosspollination  populations  can be performed using different  selection methods (mass, individual, inbreeding). The aim of this research was to develop methods of elite seed-growing population for Carnation Turkish. Source material served as seeds superdwarf  population  "Early Dwarf Mixed", derived  from  FSBSI "All-Russian Research Institute of Medicinal and  Aromatic Plants». Research conducted on plots FSBSI «Federal Scientific  Vegetable  Center». After the population has  been  designed  for  maintaining constant these traits in  subsequent reproductions. As  a  result of breeding work has created a new different colour population entitled "Rainbow". When analyzing the cleavage "tag coloring flower" in population  "Rainbow"  Carnation Turkish within a few reproductions, it was found that: the range of variability of coloring a flower includes 5 major groups - pink, rose, red, salmon, white; red colour is dominant towards pink and salmon, respectively;  for coloring a flower meet at least 5 genes: one controls the general predecessor synthesis of Ant-pigments, 2  gene dominant responsibility  for pink and red colour, and their recessive  alleles for pink and salmon, respectively;  2 gene suppressors  are the synthesis  of Antpigments cyaniding and pelargonidin.

Since ancient times, species of the genus Allium have played a significant role in the human diet, in traditional medicine for the treatment of many diseases and in official medicine as raw materials, as well as medicinal and prophylactic agents. Phytochemicals such as organosulfur compounds, phenolic compounds, fatty acids and saponins are associated with the antioxidant and antimicrobial properties of these species, among many other biologically active substances. All parts of the plant, including the bulb, leaf, pseudostem, root, flower and seed, exhibit antioxidant properties in in vitro assays. Characteristic phytocompounds that contribute to the antimicrobial activity of onion crops include allicin, ajoene, allyl alcohol and some diallyl sulfides. Nanoparticles synthesized using Allium species are also known for their notable antimicrobial properties. Allium species are a product with high nutritional value, and due to secondary metabolites, it is also used to protect plants with various products created on the basis of its biologically active components. Allium species are valued for their unique macroelement composition and are used as popular vegetables and spices. In the Russian Federation, there is significant biological and morphological diversity of cultivated and wild onion species. Over the past few years, an extensive program of collecting and studying Allium species has been carried out at VNIIO – a branch of the FSBSI Federal Scientific Vegetable Center. High morphological and biochemical variability of the studied species has been established, which can be used in breeding programs and when growing varieties.

Microgreens are valued for their freshness and refined taste, and by adherents of a healthy diet – for their saturation with vitamins, trace elements, antioxidants, enzymes and valuable protein. The product enjoys steadily growing demand not only in the production segment (restaurants, cafes, supermarkets), but also in home cultivation – «vegetable garden on the window». Among vegetable crops, vegetable pea micro-greens are very popular, especially varieties with a mustachioed leaf type. It is this product that helps culinary masters to give products bright visual and taste accents. Few people know that among legumes, in addition to vegetable peas, there is an excellent alternative with no less nutritional value. This is a micro-green of vegetable beans. Bean shoots are more fleshy and juicy, crispy, sweet with a nutty taste, and most importantly, absolutely everyone can grow them. Obtaining microgreens from vegetable bean culture is a promising direction, since its nutritional value and biochemical composition are comparable to the microgreens of vegetable peas. The content of the main nutrients on average for the studied varieties of breeding of the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC) is: protein – 20-35% (dry weight), ascorbic acid – 0,51 mg/g, carotenoids – 0,48 mg/g, monosaccharide – 1,15%, dry matter – 10,62%. However, vegetable beans have an undeniable advantage in cultivation – it is the possibility of using up to two or three cuts from one crop, due to the ability to form additional shoots when using the method of cutting under the «root». The yield from the container when sowing 100 seeds obtained in two cuts ranged from 320 g to 400 g, depending on the variety and method of cutting. The highest yield was obtained from the cv. Velena, which forms about 200 g of fresh shoots both in the first and second cut. According to the results of our research, it is recommended to use two cuts for the cv. Belorusskie and cv. Russkie chernye, while the cv. Velena is able to give a full-fledged microgreen even with the third cut.

Relevance Traditional breeding methods are based on crossing and selection of genotypes among hybrid offspring. In recent decades, along with traditional methods, more and more attention is paid to alternative methods of selection, based on biotechnological manipulations with plants. One of the most important methods of biotechnology is the method of cell selection, which is based on the replacement of the whole plant, as a unit of selection, on its cell. Applying biotechnology techniques from a single plant can produce millions of cells, which increases the chances of finding, eliminating the need for areas for the cultivation of tested plants. As well as accelerating the selection process due to the possibility to carry out the study in the offseason. Methods The studies used the linear material of C. sativus hybrids of All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center and Agroholding "Poisk". Plants were cultivated in laboratory room conditions. As explants used hypocotyl 0.5-1 cm segments isolated from young plants. Results To obtain Cucumis sativus plants with increased resistance to Fusarium by cell selection method, it is recommended to alternate culturing of callus on a non – selective medium containing sucrose in a concentration of 30 g/l, agar – 7 g/l, 0.1 mg/l, NUC – 0.5 mg/l and the filter of the cultural fluid of the fungus in a concentration of 10% within 3 passages.

When working with pollen, it is important to keep its functional parameters for some time. To do this, it is necessary to select the best conditions to preserve microgametophyte germination. The influence of two methods of pollen collection and storage of inbred plants of beetroot on their functional characteristics, at germination on an artificial nutrient medium under in vitro conditions was studied. In the first variant pollen was harvested from full-blown flowers in sealed sample bottles, in the second variant small branches with flowers and large buds were separated from inflorescence, placed in paper and then in polyethylene bags and stored till germination in a refrigerated heating circulator at 10-12 deg. C (control is freshly harvested pollen). It is shown that when storing pollen in sample bottles, the pollen viability decreased 3-10 times depending on a sample compared to the initial value already on the second or third days, and after a week of storage, pollen conglomerated, the number of burst pollen grains in the preparation increased. When stored in flowers and in branch buds on the seventh to eighth days, the decrease in pollen viability was no more than 30% of the control, and the growth rate of the pollen tube in most samples remained approximately at the control level. And the sample distribution pattern as to microgametophyte viability retained. The linear dependence covariance coefficient between the viability values before and after week-long storage was R2=0.94. That is, for evaluating the functional microgametophyte parameters of inbred beetroot plants , it is recommended to store pollen directly in branches of blossom placed in paper and then in polyethylene bags at a reduced temperature of 10-12 deg C. This is important when working with a large set of samples where it is not possible to compare them at the same time. | Важным условием при работе с пыльцой является сохранение ее функциональных параметров в течение определенного времени, для чего необходимо подобрать оптимальные условия, позволяющие сохранять жизне- и оплодотворяющую способность микрогаметофита. С этой целью изучено влияние двух способов сбора и хранения пыльцы инбредных растений свеклы столовой на ее функциональные характеристики при проращивании на искусственной питательной среде в условиях in vitro. В первом варианте пыльцу собирали с распустившихся цветков в герметично закрываемые бюксы, во втором – отделяли от соцветия небольшие веточки с цветками и крупными бутонами, помещали их бумажные, а затем в полиэтиленовый пакеты и хранили до проращивания в хладотермостате при 10…12 град. С (контроль – свежесобранная пыльца). Показано, что при хранении пыльцы в бюксах уже на вторые-третьи сутки жизнеспособность (ЖСП) в зависимости от образца снижалась в 3-10 раз по сравнению с исходным значением, а после недельного хранения она слипалась в конгломераты, и увеличивалось число лопнувших пыльцевых зерен в препарате. При хранении в цветках и бутонах на веточках снижение ЖСП на 7-8-е сутки хранения составляло не более 30% от контроля, а скорость роста пыльцевой трубки у большинства образцов оставалась на уровне контрольной. При этом характер распределения образцов по уровню ЖСП микрогаметофита сохранялся. Коэффициент ковариации линейной зависимости между значениями ЖСП до и после недельного хранения составил R2=0,94. То есть, для оценки функциональных параметров микрогаметофита инбредных растений свеклы столовой рекомендуется хранение пыльцы непосредственно на цветущих веточках, помещенных в бумажные, а затем в полиэтиленовый пакеты, при пониженной положительной температуре 10-12 град. С. Это важно при работе с большим набором образцов, когда нет возможности провести их сравнительный анализ единовременно.

Relevance. Vegetable beans (Phaseolus vulgaris L.) – a valuable high-protein culture, which has multilateral use in the national economy, is recommended for dietary nutrition. The protein content in green beans and seeds varies from 18 to 30%, which in terms of amino acid composition is at the level of protein in milk and meat.The aim of the research is to create a new indeterminate medium-late resistant to major diseases varieties for farms growing that provide fresh produce (green beans) with a long fruiting period, without fiber and parchment in the leaflets of the green bean.Methods. Studies were carried out from 2008 to 2018 on breeding fields of the Federal Scientific Vegetable Center (Moscow Region, Odintsovo District). Scientific work was started in 2013 with an individual selection from collection sample No. 90K (Republic of Crimea). Since 2015, the constant form was propagated with a two-year test of offspring. All the necessary phenological, biometric and phytopathological observations and counts were performed according to the methods generally accepted for this culture.Results. The article presents data on a new variety of vegetable beans Malume of indeterminate (curly) type of growth. As a result of competitive variety testing in the conditions of the Central Non-chernozem Zone since 2018 to 2019, against a natural infectious background, the new variety significantly exceeded the Fatima standard for the yield of green beans and seeds, was characterized by a high content of mono-, the amount of sugars, starch, and was resistant to major diseases. At the end of 2019 year transferred to the State Variety Test.