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Screening of early diagnostic markers of gentamicin-induced acute kidney injury in canines Полный текст
2019
Zheng Jia-San | Jing-Nie | Zhu Ting-Ting | Ruan Hong-Ri | Xue-Wei | Rui-Wu
Screening of early diagnostic markers of gentamicin-induced acute kidney injury in canines Полный текст
2019
Zheng Jia-San | Jing-Nie | Zhu Ting-Ting | Ruan Hong-Ri | Xue-Wei | Rui-Wu
The value of neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (Kim-1), and liver-type fatty acid binding protein (L-FABP) was assessed in early diagnosis of gentamicin-induced acute kidney injury (AKI) in dogs.
Показать больше [+] Меньше [-]Screening of early diagnostic markers of gentamicin-induced acute kidney injury in canines Полный текст
2019
Zheng, Jia-San | Jing-Nie, | Zhu, Ting-Ting | Ruan, Hong-Ri | Xue, Wei | Rui-Wu,
The value of neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (Kim-1), and liver-type fatty acid binding protein (L-FABP) was assessed in early diagnosis of gentamicin-induced acute kidney injury (AKI) in dogs. Subcutaneous gentamicin injection in 16 healthy adult beagles made the AKI model. Blood was sampled every 6 h to detect NGAL, Kim-1, L-FABP, and serum creatinine (SCr) concentrations. Kidney tissue of two dogs was taken before the injection, as soon as SCr was elevated (78 μmol/L), and when it had risen to 1.5 times the baseline, and haematoxylin-eosin staining and transmission electron microscopy (TEM) were used to observe changes. NGAL, Kim-1, and SCr levels were significantly increased (P < 0.05) at 18, 30, and 78 h post injection, but L-FABP concentration was not associated with renal injury. At the earliest SCr elevation stage, findings were mild oedema, degeneration, and vacuolisation in renal tubular epithelial cells in pathology, and mild cytoplasmic and mitochondrial oedema in TEM. At this time point, NGAL and Kim-1 concentrations were significantly increased (P < 0.05), indicating that these two molecules biomark early kidney injury in dogs. Using receiver operating characteristic curve analysis, their warning levels were > 25.31 ng/mL and > 48.52 pg/mL. Plasma NGAL and Kim-1 above warning levels are early indicators of gentamicin-induced AKI in dogs.
Показать больше [+] Меньше [-]Tritrichomonas foetus as a causative agent of tritrichomonosis in different animal hosts Полный текст
2019
Dąbrowska Joanna | Karamon Jacek | Kochanowski Maciej | Sroka Jacek | Zdybel Jolanta | Cencek Tomasz
Tritrichomonas foetus as a causative agent of tritrichomonosis in different animal hosts Полный текст
2019
Dąbrowska Joanna | Karamon Jacek | Kochanowski Maciej | Sroka Jacek | Zdybel Jolanta | Cencek Tomasz
Tritrichomonas foetus is a protozoan parasite that has been traditionally identified as a cause of reproductive tract disease in cattle and gastrointestinal tract infection in cats. Moreover, T. foetus is also well known as a commensal of the nasal cavity, intestines, and stomach in swine. In this review we describe T. foetus as a pathogen dangerous to more than one animal host, diagnostic and taxonomic aspects of this infection, and the extent to which isolates from different hosts share genetic identity.
Показать больше [+] Меньше [-]Tritrichomonas foetus as a causative agent of tritrichomonosis in different animal hosts Полный текст
2019
Dąbrowska, Joanna | Karamon, Jacek | Kochanowski, Maciej | Sroka, Jacek | Zdybel, Jolanta | Cencek, Tomasz
Tritrichomonas foetus is a protozoan parasite that has been traditionally identified as a cause of reproductive tract disease in cattle and gastrointestinal tract infection in cats. Moreover, T. foetus is also well known as a commensal of the nasal cavity, intestines, and stomach in swine. In this review we describe T. foetus as a pathogen dangerous to more than one animal host, diagnostic and taxonomic aspects of this infection, and the extent to which isolates from different hosts share genetic identity.
Показать больше [+] Меньше [-]Impact of prepartum body condition score loss on metabolic status during the transition period and subsequent fertility in Brown Swiss dairy cows Полный текст
2019
Çolakoğlu Hatice Esra | Yazlık Murat Onur | Pekcan Mert | Kaya Ufuk | Kaçar Cihan | Vural Mehmet Rıfat | Kurt Serdal | Yildirim Merve Menekse | Bas Ahmet | Küplülü Şükrü
Impact of prepartum body condition score loss on metabolic status during the transition period and subsequent fertility in Brown Swiss dairy cows Полный текст
2019
Çolakoğlu Hatice Esra | Yazlık Murat Onur | Pekcan Mert | Kaya Ufuk | Kaçar Cihan | Vural Mehmet Rıfat | Kurt Serdal | Yildirim Merve Menekse | Bas Ahmet | Küplülü Şükrü
The objectives of this study were to determine the role of a fall in pre-calving body condition score (BCS) in postpartum metabolic status and reproductive outcomes, and gauge the indicativeness of blood metabolites during the transition period.
Показать больше [+] Меньше [-]Impact of prepartum body condition score loss on metabolic status during the transition period and subsequent fertility in Brown Swiss dairy cows Полный текст
2019
Çolakoğlu, Hatice Esra | Yazlık, Murat Onur | Pekcan, Mert | Kaya, Ufuk | Kaçar, Cihan | Vural, Mehmet Rıfat | Kurt, Serdal | Yildirim, Merve Menekse | Bas, Ahmet | Küplülü, Şükrü
The objectives of this study were to determine the role of a fall in pre-calving body condition score (BCS) in postpartum metabolic status and reproductive outcomes, and gauge the indicativeness of blood metabolites during the transition period. Cows were grouped based on BCS loss between days −14 ±3 and 0 relative to calving. Cows that lost no BCS were the BCS control group (BCS-C), cows that lost 0.25 BCS points the low BCS loss group (BCS-L), and those that lost 0.5 points or more the high BCS loss (BCS-H) group. Blood was taken on days −14 ±3, 3, 14, and 30 ±4 for determination of comprehensive metabolic panel biomarker levels. Beta-hydroxybutyric acid (BHBA) levels were quantified on postpartum examination days. Vaginal discharge scores, ovarian activity on day 30 ±4, and subsequent fertility parameters were evaluated. The BCS-H cows had lower mean Ca concentrations before calving and on day 3, when the group’s BHBA and CK were higher (P < 0.05); on day 14 they had higher AST concentrations (P < 0.05). The BCS-L cows had greater bilirubin levels (P < 0.05). The BCS-H cows had lower cyclicity and higher endometritis rates. First service pregnancy rates were 50%, 50%, and 61.9%, open days 96.8, 95.75, and 89.2, and overall pregnancy rates 56.25%, 65%, and 80.95 % in the BCS-H, BCS-L, and BCS-C groups, respectively. Prepartum BCS loss of ≥ 0.5 points could be associated with Brown Swiss cow low Ca and BHBA concentrations early postpartum, and with subsequent uterine health and overall pregnancy rate. Prepartum Ca concentration might be a prognostic biomarker for postpartum metabolic status and reproductive outcomes.
Показать больше [+] Меньше [-]Marine tetrodotoxin as a risk for human health Полный текст
2019
Madejska Anna | Michalski Mirosław | Osek Jacek
Marine tetrodotoxin as a risk for human health Полный текст
2019
Madejska Anna | Michalski Mirosław | Osek Jacek
Tetrodotoxin (TTX) is a toxin mainly occurring naturally in contaminated puffer fish, which are a culinary delicacy in Japan. It is also detected in various marine organisms like globefish, starfish, sunfish, stars, frogs, crabs, snails, Australian blue-ringed octopuses, and bivalve molluscs. TTX is produced by marine bacteria that are consumed mainly by fish of the Tetraodontidae family and other aquatic animals. TTX poisoning through consuming marine snails has recently begun to occur over a wider geographical extent through Taiwan, China, and Europe. This neurotoxin causes food intoxication and poses an acute risk to public health. The aim of this review is to present the most recent information about TTX and its analogues with particular regard to toxicity, methods of analysis, and risk to humans of exposure.
Показать больше [+] Меньше [-]Marine tetrodotoxin as a risk for human health Полный текст
2019
Madejska, Anna | Michalski, Mirosław | Osek, Jacek
Tetrodotoxin (TTX) is a toxin mainly occurring naturally in contaminated puffer fish, which are a culinary delicacy in Japan. It is also detected in various marine organisms like globefish, starfish, sunfish, stars, frogs, crabs, snails, Australian blue-ringed octopuses, and bivalve molluscs. TTX is produced by marine bacteria that are consumed mainly by fish of the Tetraodontidae family and other aquatic animals. TTX poisoning through consuming marine snails has recently begun to occur over a wider geographical extent through Taiwan, China, and Europe. This neurotoxin causes food intoxication and poses an acute risk to public health. The aim of this review is to present the most recent information about TTX and its analogues with particular regard to toxicity, methods of analysis, and risk to humans of exposure.
Показать больше [+] Меньше [-]Potential role of different fish species as vectors of koi herpesvirus (CyHV-3) infection Полный текст
2019
Matras, Marek | Stachnik, Magdalena | Borzym, Ewa | Maj-Paluch, Joanna | Reichert, Michał
Potential role of different fish species as vectors of koi herpesvirus (CyHV-3) infection Полный текст
2019
Matras, Marek | Stachnik, Magdalena | Borzym, Ewa | Maj-Paluch, Joanna | Reichert, Michał
Koi herpesvirus (KHV) has infected farmed common carp in Poland clinically and asymptomatically since 2004. The role of non-carp species as vectors of virus transmission is well known except for in the case of KHV. The aim was to better understand this virus’ infection and transmission pathways in common carp, looking at the potential vector role of fishes kept with them. Eight species were experimentally infected with KHV by immersion in a suspension at 20°C ±1 and transferred to a tank after 45 minutes. Specimens were euthanised at intervals up to 56 days post infection (dpi) and tissue was examined for KHV DNA. Surviving infected fishes were introduced at intervals, each time into a separate tank, to naïve common carp for experimental infection. These were observed daily for symptoms, sacrificed along with controls after three months, and dissected to provide tissue samples. Also fish from 14 species collected from a farm with a history of KHV were sampled from 3 to 22 months after disease was confirmed. Organ sections from single fish were collected in a single tube. Viral DNA was detected in tench and roach samples up to 49 dpi, but in three-spined stickleback and stone maroko samples only up to 14 dpi. Transmission of KHV to naïve carp occurred after cohabitation. KHV DNA was detected in three fish species three months after the farm outbreak. We confirmed that grass and Prussian carp, tench, roach, and brown bullhead can transfer the virus to naïve common carp.
Показать больше [+] Меньше [-]Potential role of different fish species as vectors of koi herpesvirus (CyHV-3) infection Полный текст
2019
Matras Marek | Stachnik Magdalena | Borzym Ewa | Maj-Paluch Joanna | Reichert Michał
Koi herpesvirus (KHV) has infected farmed common carp in Poland clinically and asymptomatically since 2004. The role of non-carp species as vectors of virus transmission is well known except for in the case of KHV. The aim was to better understand this virus’ infection and transmission pathways in common carp, looking at the potential vector role of fishes kept with them.
Показать больше [+] Меньше [-]Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status Полный текст
2019
Domosławska, Anna | Zduńczyk, Sławomir | Janowski, Tomasz
Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status Полный текст
2019
Domosławska, Anna | Zduńczyk, Sławomir | Janowski, Tomasz
Introduction: Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. Material and Methods: The dogs (a Golden Retriever, an English Cocker Spaniel, and two Tibetan Mastiffs) were supplemented daily with selenium (Se) (0.6 mg/kg organic Se yeast) and vitamin E (vit. E) (5 mg/kg) per os for 60 days. Semen was collected on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated by the CASA system, sperm morphology was explored by Diff-Quick staining, and live and dead spermatozoa were differentiated by eosin/nigrosin staining. The concentrations of Se and vit. E were measured in peripheral blood serum on semen collection days. Results: Before administration, the concentrations of Se in blood plasma were low (86.0–165.0 µg/L). After 30 days of treatment there was an observable improvement in total and progressive sperm motility and kinematic parameters (VAP, VSK, VCL, ALH, BCF, and RAPID). The percentages of live and normal morphology sperm cells were also higher. There was also an observable increase in Se and vitamin E concentrations in blood serum. Bitches were successfully mated and delivered four to six puppies. Conclusion: Supplementation with Se and vit. E improved rapid sperm motility and restored fertility in infertile dogs with low Se status.
Показать больше [+] Меньше [-]Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status Полный текст
2019
Domosławska Anna | Zduńczyk Sławomir | Janowski Tomasz
Introduction: Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. Material and Methods: The dogs (a Golden Retriever, an English Cocker Spaniel, and two Tibetan Mastiffs) were supplemented daily with selenium (Se) (0.6 mg/kg organic Se yeast) and vitamin E (vit. E) (5 mg/kg) per os for 60 days. Semen was collected on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated by the CASA system, sperm morphology was explored by Diff-Quick staining, and live and dead spermatozoa were differentiated by eosin/nigrosin staining. The concentrations of Se and vit. E were measured in peripheral blood serum on semen collection days. Results: Before administration, the concentrations of Se in blood plasma were low (86.0–165.0 µg/L). After 30 days of treatment there was an observable improvement in total and progressive sperm motility and kinematic parameters (VAP, VSK, VCL, ALH, BCF, and RAPID). The percentages of live and normal morphology sperm cells were also higher. There was also an observable increase in Se and vitamin E concentrations in blood serum. Bitches were successfully mated and delivered four to six puppies. Conclusion: Supplementation with Se and vit. E improved rapid sperm motility and restored fertility in infertile dogs with low Se status.
Показать больше [+] Меньше [-]Effect of simvastatin on thrombopoiesis in porcine bone marrow Полный текст
2019
Snarska, Anna | Wysocka, Dominika | Rytel, Liliana
Effect of simvastatin on thrombopoiesis in porcine bone marrow Полный текст
2019
Snarska, Anna | Wysocka, Dominika | Rytel, Liliana
Introduction: Statins are pharmacological agents commonly used to lower serum cholesterol level. The aim of the experiment was to investigate the effect of the statin simvastatin on thrombopoiesis in the porcine model because it is the closest to the human one regarding physiological and genetic similarities. Material and Methods: The study was conducted on a group of 32 pigs randomly divided into two equal groups: control and experimental. The pigs were treated for 28 and 56 days with simvastatin in a dose of 40 mg per day per animal. Cytological evaluation of bone marrow smears was performed to assess the average number of all types of cells during thrombopoiesis as was analysis of haematological parameters to assess PLT and MPV. Results: During the course of the experiment statistically significant changes in the number of promegakaryocytes were observed. Other parameters also showed some fluctuations during the study. However, these changes were not statistically significant. Conclusion: The obtained results clearly indicate a toxic influence of simvastatin on the process of thrombopoiesis and prove that statins reduce mean platelet volume, thus affecting the process of clot formation through the period of administration in a duration-dependent manner.
Показать больше [+] Меньше [-]Effect of simvastatin on thrombopoiesis in porcine bone marrow Полный текст
2019
Snarska Anna | Wysocka Dominika | Rytel Liliana
Introduction: Statins are pharmacological agents commonly used to lower serum cholesterol level. The aim of the experiment was to investigate the effect of the statin simvastatin on thrombopoiesis in the porcine model because it is the closest to the human one regarding physiological and genetic similarities.
Показать больше [+] Меньше [-]The use of the water treadmill for the rehabilitation of musculoskeletal injuries in the sport horse Полный текст
2019
Muñoz, Ana | Saitua, Aritz | Becero, Mireya | Riber, Cristina | Satué, Katy | Medina, Antonia Sánchez de | Argüelles, David | Castejón-Riber, Cristina
The use of the water treadmill for the rehabilitation of musculoskeletal injuries in the sport horse Полный текст
2019
Muñoz, Ana | Saitua, Aritz | Becero, Mireya | Riber, Cristina | Satué, Katy | Medina, Antonia Sánchez de | Argüelles, David | Castejón-Riber, Cristina
In recent years, exercise on a water treadmill has come to have great relevance in rehabilitation and training centres for sport horses. Its use exploits certain physical properties of water, related to the fundamental principles of hydrodynamics, such as buoyancy, viscosity, hydrostatic pressure, and water temperature. These properties together with deliberate specification of the depth of the water and the velocity of the treadmill provide a combination of parameters that can be varied according to the purpose of the rehabilitation or training programme, the disease to rehabilitate, or the healing phase. In the current article, kinematic adaptations to exercise on a water treadmill and the direct application of such exercise to the rehabilitation of superficial and deep digital flexor tendon and accessory ligament injuries and back and joint diseases are described.
Показать больше [+] Меньше [-]The Use of the Water Treadmill for the Rehabilitation of Musculoskeletal Injuries in the Sport Horse Полный текст
2019
Muñoz, Ana | Saitua, Aritz | Becero, Mireya | Riber, Cristina | Satué, Katiuska | Sánchez de Medina, Antonia | Arguelles, David | Castejón-Riber, Cristina
In recent years, exercise on a water treadmill has come to have great relevance in rehabilitation and training centres for sport horses. Its use exploits certain physical properties of water, related to the fundamental principles of hydrodynamics, such as buoyancy, viscosity, hydrostatic pressure, and water temperature. These properties together with deliberate specification of the depth of the water and the velocity of the treadmill provide a combination of parameters that can be varied according to the purpose of the rehabilitation or training programme, the disease to rehabilitate, or the healing phase. In the current article, kinematic adaptations to exercise on a water treadmill and the direct application of such exercise to the rehabilitation of superficial and deep digital flexor tendon and accessory ligament injuries and back and joint diseases are described
Показать больше [+] Меньше [-]The use of the water treadmill for the rehabilitation of musculoskeletal injuries in the sport horse Полный текст
2019
Muñoz Ana | Saitua Aritz | Becero Mireya | Riber Cristina | Satué Katy | Medina Antonia Sánchez de | Argüelles David | Castejón-Riber Cristina
In recent years, exercise on a water treadmill has come to have great relevance in rehabilitation and training centres for sport horses. Its use exploits certain physical properties of water, related to the fundamental principles of hydrodynamics, such as buoyancy, viscosity, hydrostatic pressure, and water temperature. These properties together with deliberate specification of the depth of the water and the velocity of the treadmill provide a combination of parameters that can be varied according to the purpose of the rehabilitation or training programme, the disease to rehabilitate, or the healing phase. In the current article, kinematic adaptations to exercise on a water treadmill and the direct application of such exercise to the rehabilitation of superficial and deep digital flexor tendon and accessory ligament injuries and back and joint diseases are described.
Показать больше [+] Меньше [-]Virulence factors and antibiotic resistance of avian pathogenic Escherichia coli in eastern China Полный текст
2019
Xu, Xiaojing | Sun, Qing | Zhao, Lixiang
Virulence factors and antibiotic resistance of avian pathogenic Escherichia coli in eastern China Полный текст
2019
Xu, Xiaojing | Sun, Qing | Zhao, Lixiang
Avian pathogenicEscherichia coli (APEC) causes serious colibacillosis and significant economic losses. Data on profiles of virulence factors and antibiotic resistances among APEC strains are crucial to the control of infection. In this study, strains were isolated from eastern China, and the prevalence of virulence factors and distribution of antibiotic resistance were determined. APEC strains were isolated and characterised by PCR for O serogroups, virulence factor genes, antibiotic resistance, and phylogenetic groups. O78 was the most prevalent serogroup and type A was the most frequent phylogenetic group. ThefimH,feoB, andiron genes were the most prevalent among the isolates. All isolates were multiresistant, and all strains were resistant to ampicillin and tetracycline, which are widely used in the poultry industry in China. This study provided important data on the presence of virulence genes and antibiotic resistance profiles of APEC from poultry farms in eastern China.
Показать больше [+] Меньше [-]Virulence factors and antibiotic resistance of avian pathogenic Escherichia coli in eastern China Полный текст
2019
Xu Xiaojing | Sun Qing | Zhao Lixiang
Avian pathogenicEscherichia coli (APEC) causes serious colibacillosis and significant economic losses. Data on profiles of virulence factors and antibiotic resistances among APEC strains are crucial to the control of infection. In this study, strains were isolated from eastern China, and the prevalence of virulence factors and distribution of antibiotic resistance were determined.
Показать больше [+] Меньше [-]Seroprevalence of Q fever in sheep and goats from the Marmara region, Turkey Полный текст
2019
Karagul, Mustafa Sencer | Malal, Mehmet Engin | Akar, Kadir
Seroprevalence of Q fever in sheep and goats from the Marmara region, Turkey Полный текст
2019
Karagul, Mustafa Sencer | Malal, Mehmet Engin | Akar, Kadir
The aim of this study was to investigate Q fever seroprevalence in sheep and goats in the Marmara region. Q fever is a zoonotic disease caused by Coxiella burnetii. In ruminants, the disease causes reproductive disorders, premature births and stillbirths. Blood samples of sheep and goats were collected from the Marmara region of Turkey and a commercial ELISA was used for detection of specific antibodies to C. burnetii. A total of 832 samples (627 from sheep and 205 from goats) obtained from 126 herds located in 110 villages in 63 municipalities across all 11 provinces were utilised. Total seroprevalence was found to be 13.22%, while the proportion of seropositive herds was determined to be over threefold higher at 42.85%. The seroprevalence for sheep was found to be 14.19%, and for goats 10.24%. The herd seropositivity rate for sheep of 46.31% and for goats of 32.25% were also over threefold higher than the species-level seroprevalences. The provincial seroprevalence varied between 1.38% and 21.79%. This study confirms the presence of C. burnetii in sheep and goat herds in the Marmara region and provides original seroprevalence data in hitherto uninvestigated provinces. The data gathered are beneficial for evaluation and elaboration of the seroprevalence of Q fever in sheep and goats in the Marmara region. Surveillance studies should be maintained, particularly in provinces with high seropositivity rates.
Показать больше [+] Меньше [-]Seroprevalence of Q fever in sheep and goats from the Marmara region, Turkey Полный текст
2019
Karagul Mustafa Sencer | Malal Mehmet Engin | Akar Kadir
The aim of this study was to investigate Q fever seroprevalence in sheep and goats in the Marmara region. Q fever is a zoonotic disease caused by Coxiella burnetii. In ruminants, the disease causes reproductive disorders, premature births and stillbirths.
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