Уточнить поиск
Результаты 101-110 из 606
Influence of neurokinin B, dynorphin A and kisspeptin-10 on in vitro gonadotropin secretion by anterior pituitary cells isolated from pubescent ewes Полный текст
2025
Szysiak Natalia | Kosior-Korzecka Urszula | Longo Vincenzo | Patkowski Krzysztof | Greguła-Kania Monika | Nowakiewicz Aneta | Bochniarz Mariola | Junkuszew Andrzej
The hypothalamic neuropeptides kisspeptin-10 (KiSS-10), neurokinin B (NKB), and dynorphin A (Dyn A) play roles in the endocrine regulation of the hypothalamic–pituitary–ovarian (HPO) axis in puberty. Livestock’s timely attainment of sexual maturity increases reproductive efficiency and raises profitability. The pituitary relationship between these neuropeptides and gonadotropins in puberty in ewes being undercharacterised. The aim of the study was to analyse their direct effect on gonadotropin secretion by pituitary cells isolated from pubescent ewes.
Показать больше [+] Меньше [-]Molecular detection and characterisation of herpesviruses in asymptomatic Russian sturgeon (Acipenser gueldenstaedtii) from European aquaculture Полный текст
2025
Bergmann Sven Michael | Todte Matthias | Jäger Lea | Lorenzen-Schmidt Fermin Georgio | Jin Yeonwha | Klafack Sandro | Lenk Matthias | Syahidah Dewi | Hastilestari Bernadetta Rina | Penetaseputro Tanjung | Avare Jean-Christophe | Hwang Jeeyoun | Kiełpińska Jolanta
Molecular detection and characterisation of herpesviruses in asymptomatic Russian sturgeon (Acipenser gueldenstaedtii) from European aquaculture Полный текст
2025
Bergmann Sven Michael | Todte Matthias | Jäger Lea | Lorenzen-Schmidt Fermin Georgio | Jin Yeonwha | Klafack Sandro | Lenk Matthias | Syahidah Dewi | Hastilestari Bernadetta Rina | Penetaseputro Tanjung | Avare Jean-Christophe | Hwang Jeeyoun | Kiełpińska Jolanta
In Germany, around 150,000 kg of mostly Siberian sturgeon (Acipenser baerii), were produced in 2021. Sudden mortalities affected negative control Russian sturgeon in experimental infection of several European aquacultured fish species with tilapia lake virus (TiLV). An investigation sought the causative agent. In most of the sturgeon, a specific herpesvirus was detected which also occurred in the carp, crucian carp and tench subjects, but not in Nile tilapia. This herpesvirus was latent in the sturgeon population but became productive to cause the outbreak after three weeks of experimentation.
Показать больше [+] Меньше [-]Molecular detection and characterisation of herpesviruses in asymptomatic Russian sturgeon (Acipenser gueldenstaedtii) from European aquaculture Полный текст
2025
Bergmann, Sven Michael | Todte, Matthias | Jäger, Lea | Lorenzen-Schmidt, Fermin Georgio | Jin, Yeonwha | Klafack, Sandro | Lenk, Matthias | Syahidah, Dewi | Hastilestari, Bernadetta Rina | Penetaseputro, Tanjung | Avare, Jean-Christophe | Hwang, Jeeyoun | Kiełpińska, Jolanta
Introduction In Germany, around 150,000 kg of mostly Siberian sturgeon (Acipenser baerii), were produced in 2021. Sudden mortalities affected negative control Russian sturgeon in experimental infection of several European aquacultured fish species with tilapia lake virus (TiLV). An investigation sought the causative agent. In most of the sturgeon, a specific herpesvirus was detected which also occurred in the carp, crucian carp and tench subjects, but not in Nile tilapia. This herpesvirus was latent in the sturgeon population but became productive to cause the outbreak after three weeks of experimentation. Material and Methods Different European aquacultured fishes were experimented upon. Chosen PCRs, nested PCRs and re-amplifications were carried out to identify the causative agent of the mortality event. Sequence analysis of the obtained PCR fragment and in-situ hybridisation (ISH) using tissue sections of the experimental fishes were performed. Results The PCRs used for detection of Acipenser herpesvirus (AciHV)-1 and -2 were always negative. An additional PCR assay with lesser specificity for AciHV found 118 of 123 sturgeon samples and some samples of cohabited cyprinids positive. The similarity of all isolates was 99.7%. The PCR results were confirmed by ISH using probes based on the same sequence, which detected identical viral sequences in both sturgeon and cyprinid samples. These findings revealed that a sterlet sequence previously deposited in the NCBI database had been incorrectly classified. Conclusion It seems that different herpesviruses and/or a new subspecies of AciHV are widespread in European farmed sturgeon populations, which, at least for aquacultured fish, opens up the possibility of vaccination against the disease which they cause. Additionally, a more specific diagnostic PCR has to be established.
Показать больше [+] Меньше [-]Proteomic insights into nematode-trapping fungi Arthrobotrys oligospora after their response to chitin Полный текст
2025
Zhang Jiahua | Wei Lixiang | Zhang Huimei | Ma Xixi | Sun Yansen | Li Ruobing | Zhang Chengzhi | Cai Xuepeng | Qiao Jun | Meng Qingling
Nematode-trapping fungi (NTFs) can produce various chitinases to degrade nematode body wall and eggshell chitin during predation. However, the regulatory mechanisms of their expression of chitinases still remain unclear. The primary objective of this study was to elucidate the differential protein profile of A. oligospora, an NTF, in response to chitin.
Показать больше [+] Меньше [-]Effect of probiotics on immune cells in young Japanese Black calves responding to vaccination against bacterial respiratory diseases Полный текст
2025
Takeda Shogo | Ohtsuka Hiromichi | Kosenda Keigo
The vaccination against bacterial respiratory diseases in calves has been generally recognised as useful for the prevention of infections. Inducing an immunological response after vaccination is important for obtaining protection from infections. The aim of the study was to investigate the effects of probiotics on the immunological response to vaccination against bacterial respiratory diseases in young Japanese Black calves.
Показать больше [+] Меньше [-]Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test Полный текст
2025
Mickiewicz Marcin | Nowek Zofia | Czopowicz Michał | Moroz-Fik Agata | Potărniche Adrian-Valentin | Biernacka Kinga | Szaluś-Jordanow Olga | Górski Paweł | Antonopoulos Alistair | Markowska-Daniel Iwona | Várady Marián | Kaba Jarosław
Dimethyl sulfoxide (DMSO) is an amphipathic solvent for molecules in in vitro tests for detection of anthelmintic resistance of gastrointestinal nematodes (GIN). It has been shown to have a concentration-dependent detrimental effect on Caenorhabditis elegans, a free-living nematode. If GIN are likewise affected, using DMSO in egg-hatch test and larval development test (LDT) may confound their results. Therefore, the DMSO concentration was determined at which it exerted an inhibitory effect on GIN larval development to the third stage.
Показать больше [+] Меньше [-]Remodelling of the healthy foal’s conjunctival microbiome in the first two months of life Полный текст
2025
Płoneczka-Janeczko Katarzyna | Armstrong Eve | Siemieniuch-Tartanus Marta | Magdziarz Marcin
The aim of the study was to explore and characterise healthy foals’ eye microbiomes in the first two months of life.
Показать больше [+] Меньше [-]The course of pigeon circovirus infection in young pigeons experimentally kept under conditions mimicking the One Loft Race rearing system Полный текст
2025
Dziewulska Daria | Tykałowski Bartłomiej | Łukaszuk Ewa | Stenzel Tomasz
Racing pigeon competitions are a popular sport where success depends on birds’ ability to return fast to their loft of origin. However, many additional factors like differences in feeding, training, everyday care and even geographical loft location influence race outcomes, which has led to the development of the One Loft Race (OLR) system. The OLR system aims to eliminate these factors by housing pigeons from various lofts in equal conditions in one facility. This in turn, however, fosters inter-individual transmission of pathogens.
Показать больше [+] Меньше [-]Effects of lipopolysaccharide administration on thymus damage, antioxidant capacity and immune function in weaned piglets Полный текст
2025
Bai Lingna | Jiang Yijie | Li Xi | Yu Wanting | Zhu Wenlu | Zhao Guotong | Yang Tingyu | Zhou Yunxiao | Li Jinyan | Li Yong
Piglets are vulnerable to stress during weaning because of changes in the feeding environment, nutrients, and other growth-impacting conditions. In this study, stress injury was modelled by continuous intraperitoneal injection of lipopolysaccharide (LPS) and was used to investigate the dynamics of antioxidant indices and immunoinflammatory factors in the piglet thymus.
Показать больше [+] Меньше [-]Characteristics of drug-resistant staphylococci isolated from milk of lambed ewes during the perinatal period Полный текст
2025
Hahaj-Siembida Agata | Nowakiewicz Aneta | Greguła-Kania Monika | Bochniarz Mariola | Trościańczyk Aleksandra | Osińska Marcelina
Staphylococci are still a challenge in veterinary medicine, as they are one of the aetiological factors causing clinical and subclinical mastitis in small ruminants. The aim of the study was to analyse the occurrence of staphylococci in milk obtained from Świniarka (SW) and Uhruska (UHR) sheep and to characterise their drug resistance and virulence.
Показать больше [+] Меньше [-]Is Pseudomonas aeruginosa a possible aetiological agent of periodontitis in dogs? Полный текст
2025
Targońska-Karasek Małgorzata | Polkowska Izabela | Krukowski Henryk
Periodontal diseases are the most frequently diagnosed problem in small animal veterinary medicine. Although their exact cause is not fully understood, bacteria play an important role in their development. Pseudomonas aeruginosa is a Gram-negative, rod-shaped, non-spore-forming bacterium. The living environment of this bacterium may be soil and water; however, it can also be found in humans and animals. Antibiotic treatment of periodontitis may be complicated by the carbapenem resistance of some P. aeruginosa strains, if these bacteria are found to be an aetiological agent. The aim of the study was to identify all bacterial strains isolated from dog with periodontitis.
Показать больше [+] Меньше [-]