The current study was designed to investigate the impacts of heat stress (HS) on performance parameters of broiler chicken reared at different stocking densities, also study assessed the effects of anti-stress (vitamin) supplementation in the mitigation of different levels of stress. A total of 720 7th-day-old Cobb ® chicks were randomly distributed into 18 groups, (two replicates within each group). The experiment with a factorial arrangement of treatments (3x3x2), 3 levels of stocking densities (RSD: 10 chicks/m2, MSD: 14 chicks/m2, and HSD: 18 chicks/m2), 2 levels of vitamin supplementation (0 mg/l and combination of 250 mg/l ascorbic acid plus 0.5 ml/l Vit E + Se) and three temperatures (Thermoneutral temperature (TN), sudden chronic heat stress exposure (CHS) and gradual chronic heat stress exposure). Broilers were kept either under thermoneutral conditions (24 ± 1 ºC) during the whole life period or slowly introduced to CHS from 7th to 21st d of age and kept at high temperature thereafter and the third chamber had chicks that were exposed to CHS (32 ± 2 ºC for 8 h/day) during the period from 21st: 42nd day of age. Chicks were reared on a deep litter system and had free access to feed and water. Performance parameters (FI, BW, BWG, and FCR) were determined on the 42nd day of age. The results showed HSD had adverse effects on the growth performance of broilers reared under thermoneutral or CHS conditions where the differences between densities were significant (P<0.05) under TN and sudden CHS conditions and insignificant in case of gradual CHS exposure conditions for most of the performance parameters. Vitamins supplementation had improved growth performance (BW and FCR) of broilers kept under MSD or HSD and exposed to thermoneutral or sudden CHS conditions as compared to corresponding not supplemented birds. While it was effective in combating the adverse effects of gradual HS exposure in RSD and MSD kept broilers only. In addition, there was no significant difference between RSD not supplemented broilers and MSD-supplemented birds under TN conditions; concluding that broilers can be stocked at MSD under thermoneutral conditions if they were supplemented with vitamins.

The study was achieved to show the influence of supplementing different concentrations of parsley and cilantro extracts on sensory and microbiological parameters of soft cheese during 2 weeks of storage. The antibacterial activity of crude extracts and the Minimum Inhibitory Concentration (MIC) of both plants were determined. Also, a study of food poisoning bacteria survival in artificially contaminated soft cheese. The Escherichia coli (E. coli), Salmonella Typhimurium (S. Typhimurium), and Staphylococcus aureus (S. aureus) were sensitive to oil extract (0.5%) of both plants, while E. coli and S. Typhimurium were sensitive to methanol extract (5.0%) of cilantro based on MIC. The oil extract (0.5%) of parsley had a significant (p<0.05) improving effect on the sensory parameters of samples throughout storage. In E. coli and S. Typhimurium contaminated samples, there was a significant difference (p<0.05) between samples with the oil extract (0.25% and 0.5%) of parsley and samples without extracts for pH value at end of the storage period. Based on the survival results, S. Typhimurium could not be isolated from all cheese samples with oil extract (0.5%) of cilantro on days 12 and 14 of storage, while E. coli count was reduced to 2.85 and 2.36 log CFU/g in cheeses with oil extract (0.25%) of parsley and cilantro, respectively. The S. aureus could not be isolated from all cheese samples with oil extract (0.5%) of parsley and cilantro from day eight till the end of the storage period. Results of the study recommended the use of these plants oil extracts to improve soft cheese safety and quality. This trial of the survival of food poisoning bacteria in the soft cheese supplemented by oil and methanol extracts of parsley and cilantro is one of the fewest studies, particularly in the Middle East.

Pseudomonas aeruginosa (P. aeruginosa) is one of the most common causative agents causing mastitis in dairy cattle, which is primarily responsible for the dairy farms' significant economic losses. The purpose of this study was to investigate the prevalence, antimicrobial sensitivity profiling, phenotypic detection of extended-spectrum ß-lactamase (ESBL) producing isolates, molecular identification of major virulence factors (toxA, lasB, and exoS), and biofilm profiles of P. aeruginosa strains isolated from mastitis milk. A total of 200 mastitic milk samples were collected from cows and subjected to bacteriological examination. Suspected colonies were confirmed by PCR targeting the 16S rRNA gene. Positive isolates were genotypically examined to determine the presence of virulence factors (toxA, lasB, and exoS). An antimicrobial susceptibility test was carried out on all positive isolates. The phenotypic detection of ESBL was done using the combination disc test (CDT) and the double disc synergy test (DDST). Finally, biofilm production was accessed through the tube adherent method (TA). Among the total samples, P. aeruginosa was identified in 15% (n = 30) of the retrieved isolates. Virulence genes (toxA, exoS, and lasB) were detected in 100%, 83.3%, and 66.6% of isolates, respectively. Antimicrobial susceptibility testing showed high sensitivity to imipenem, meropenem, ciprofloxacin, colistin, and gentamicin, while all isolates were completely resistant to amoxicillin, penicillin, ceftazidime, ceftriaxone, streptomycin, erythromycin, doxycycline, and spectinomycin. CDT confirmed ESBL production in 15 isolates (50%) and DDST confirmed it in 10 isolates (33.3%). Biofilm formation by the TA method revealed that 33.3% were strong, 33.3% were moderate, 16.6% were weak, and 16.6% were nonadherent isolates. In conclusion, P. aeruginosa strains on dairy farms that harbor virulence genes, produce biofilm, and are resistant to the most popular antimicrobial drug can be hazardous not only to the dairy industry but also for public health.

The aim of this work was to monitor the role of some risk factors on the incidence of endometritis in the herd, in addition to investigating the ability to make early pre-partum forecasting for occurrence of endometritis. 110 Holstein Friesian cows aged from 3-5 years were enrolled in this study. Cytological examination using a cytobrush (CB) and Indian MAP rapid stain kit (Indian MAP scientific©) for counting of polymorphonuclear cells (PMN) under microscope was used as the most accurate tool to discriminate between healthy cows and those affected with subclinical endometritis (SCE) at either 21 or 45 days postpartum (dpp). Serum analysis was used to measure the level of Albumin and Haptoglobin (Hp) in serum of dam at day 7 before parturition and at 21 and 45 dpp. Male calf and rainy season of parturition have a significant effect on the incidence of endometritis (P <0.05). According to our study results, the prevalence of CE (N=15) is 16.48%, whereas that of SCE (N=34) is 37.36%. Albumin (a negative acute-phase protein) level has been discovered to be a trustworthy predictor of sickness risk in transition period and fresh cows. The standard range of Albumin in cow’s serum is (2.8-3.9 g/dl). Albumin was markedly higher in healthy cows than diseased cows either prior to parturition or after 21 and 45 days in milk (DIM), The pre-partum Hp concentration between the SCE and healthy groups in this study was significantly different in a deceptive way. Hp concentration in the SCE group, however, was significantly different from the control group at 21 DIM (0.10±0.01 g/l vs. 0.05±0.002 g/l). So, Hp concentration is a satisfactory diagnostic, not a prognostic test.

Cryopreservation is very important technique in AI centers of stallions, it preserves sperms for long period and spread the superior genetic merits between different animal’s breeds. However, using of cryopreserved sperms lead to decreasing the fertility between animals, due to lethal damage of sperms during preservation process; so, this study aimed to use the two different freezing media with decreasing post thawing sperm damage. A total of 54 ejaculates were collected from nine pure fertile Egyptian Stallions (6 ejaculates per stallion), individually housed at a Veterinary Clinic in Giza Government. Semen sample was collected by Missouri AV on a regular basis (two collections ⁄ week) during the 2021 breeding season in presence of teaser mare. The collected ejaculates were sent to the laboratory immediately for evaluation by CASA (total concentration, progressive motility, static motility, and sperm abnormalities). Ejaculates were filtrated for removal gel fraction; filtrated ejaculates were diluted by EDTA-glucose media for centrifugation and the resulting sperm pellets were split into 2 equal aliquots and then extended in freezing media. INRA96 Milk-based Extender with glycerol and Egg yolk- based Extender with DMF (dimethylformamide) were used in this study as freezing extenders. Diluted semen was packaged into 0.5 ml straw then cooling at 4 ◦C and freezing by vapor of liquid nitrogen and after that preserved by freezing in liquid nitrogen container at -196⁰ C. After keeping the frozen straws in liquid nitrogen for one week, at least 2 straws were taken for thawing and evaluating post thawing-freezing motility. Finally thawed-frozen semen was inseminated inside fertile mares for calculation the conception rate after one month. Post thawing motility were evaluated in extended semen by two different extenders. The obtained results showed a change in the motility by decreasing in INRA-diluted semen compared to DMF-diluted semen. Conception rate was recorded after insemination and showed a high significant in DMF-diluted semen than INRA diluted semen. We concluded that the frozen semen with DMF based diluent did not decrease the motility of sperms after thawing and achieved high conception rate when compared with INRA based glycerol diluent.

With increased consumer knowledge about the relationship between diet and a healthy lifestyle in recent decades, there has been an increase in awareness and demand for efficient protein sources. Rabbit meat is rich in easily digestible protein, polyunsaturated fatty acids (PUFAs), vitamins, and minerals (such as calcium, magnesium, and zinc), as well as being low in fat, sodium, and cholesterol, which makes it of good edible and nutritional value, so it needs to be handled properly. Rabbit meat, on the other hand, has been associated with the spread of microbial contamination (such as Staphylococcus aureus, E. coli and Salmonella species) that may originate from the animal itself, equipment, workers, or the surrounding environment. Each effort must be made to keep rabbit meat free of contaminants that could be harmful to humans. Few studies had been conducted, either internationally or in Egypt, to examine the physicochemical and microbiological characteristics of rabbit meat. We shall compile existing data on the quality of rabbit meat in this review. Furthermore, the potential hazards that influence the physicochemical and microbiological qualities and shelf life, as well as the proposals for decontamination trials to enhance the sensory attributes and shelf life of rabbit meat, will be presented.

Diabetes is a series of metabolic conditions which threaten public health, caused by a defect in insulin secretion by the pancreatic β-cells or insulin-sensitive tissues that fail to respond to insulin leads to hyperglycemia, which causes a series of metabolic signaling pathways leading to inflammation, cytokine production, cell death, and diabetic complications. Recent research has pointed to Histidine-containing dipeptides (HDPs) to be one of the routes to enhancing diabetic complications. HDPs are synthesized in muscle and are abundantly found in mammals and other vertebrates. L-carnosine (CAR), Anserine, and homocarnosine are dipeptides produced by vertebrate muscles. Carnosine and anserine have both antiglycation and antioxidant activity that help to enhance metabolic dysregulation caused by diabetes. In addition, homocarnosine has anti-inflammatory activity, as well as the ability to reduce DNA damage and advanced glycation end products (AGEs). This review will focus on the protective effects of HDPs against diabetic complications, especially carnosine.

The domestic rabbit is a commonly kept pet in many countries, including Norway. This study aimed to survey the current health and welfare of pet rabbits in Norway based on recommendations for rabbit keeping put forth by the Norwegian Food Safety Authority and the Norwegian Veterinary Institute. An online questionnaire consisting of 65 questions was shared online. Data collection lasted from March to May of 2021 and yielded 513 responses from pet rabbit owners representing all Norwegian counties. Forty-five percent of the rabbits were female, 53.6% were male and most of the rabbits (76.0%) were neutered. Almost all rabbits (97.5%) had permanent access to hay. Complete pelleted feed was the most fed concentrate (n=492, 95.9%). Almost all rabbits (96.9%) were reported to be healthy at the current moment. Of the surveyed disease conditions, constipation (19.7%), diarrhoea (12.3%), and reduced appetite (17.9%) were the most common. Dental disease was reported in 29 rabbits (5.7%). The veterinarian was a source of information for 49.1% of rabbit owners. Most rabbits (86.2%) were kept in a living space above the recommended 200x300 cm. Although keeping conditions were reported to be of a high standard, pet rabbits were still suffering from preventable husbandry-related diseases. Education of rabbit owners is an important task of the veterinarian and should focus especially on feeding habits, and owner recognition of rabbit behaviour and diseases.

Sporotrichosis is a subcutaneous mycosis caused by species of the genus Sporothrix that has stood out in recent years, mainly in Brazil. Its importance for public health as an emerging disease is mainly associated with zoonotic transmission by domestic cats, which have a high fungal load on skin lesions, facilitating the dissemination of the agent. Classical transmission is caused by traumatic inoculation of the fungus in a contaminated environment. Propagation between animals occurs due to close contact between cats with other cats or dogs and for humans associated with bites, scratches, and interaction with exudate from infected lesions. The clinical manifestations in felines are variable and can present as localized or fixed, disseminated, cutaneous-lymphatic, and systemic. In human patients, mainly immunosuppressed, extracutaneous forms can occur, evolving even to fatal cases. Early diagnosis is essential to ensure that preventive measures are employed for owners, with the isolation of the etiological agent in culture being considered the gold standard. The lack of strategic plans, adequate control measures, failure to recognize and neglect the disease increases the incidence of cases, especially in more precarious population groups. Thus, due to its importance in public and animal health, the objective of this review was to highlight recent advances concerning the feline disease.

Bovine dictycaulosis is a serious disease in ruminants caused by lungworm Dictyocaulus viviparous with a wide spectrum of clinical manifestations. The infection is closely related to pasture contamination with the infective larvae. A cross-sectional study was conducted from July 2020 to June 2021 to detect the prevalence of bovine dictyocaulosis in two animal-rearing stations in Assiut, Upper Egypt with risk factor analysis. Also, the role of filth flies in the transmission of infective larvae was investigated. A total of 251 animals (143 cattle and 108 buffaloes) were examined using the modified Baermann technique and postmortem examination. Multiple fly pools were also collected and examined for the detection of Dictyocaulus larvae in different seasons microscopically and by PCR techniques. The overall prevalence of D. viviparus infection in cattle and buffaloes was 20.98% and 52.78%, respectively. The infection was significantly higher in the young aged and symptomatic animals (96.7%). Most lungworm infection was recorded during autumn, spring, and winter seasons, respectively. Coprological examination coincides with microscopic examination of fly wash regarding the prevalence and the seasonal variation. Musca domestica, Borborillus vitripennis, Fannia canicularis, and Sepsis punctum were the most abundant fly species and showed the highest rates of infection. To the authors’ knowledge, this is the first report of an outbreak of bovine dictycauolosis in Upper Egypt. The obtained results proved the role of filth flies in the transmission of D. viviparus larvae during the outbreak. Control programs for bovine dictyocaulosis should include the control of filth flies around the rearing places.