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Genetic changes in pigeon paramyxovirus type-1 induced by serial passages in chickens and microscopic lesions caused by the virus in various avian hosts Полный текст
2018
Olszewska-Tomczyk Monika | Dolka Izabella | Świętoń Edyta | Śmietanka Krzysztof
Introduction: Genotype VI of avian avulavirus 1 (AAvV-1) has pigeons and doves as its reservoir and is often termed pigeon paramyxovirus type-1 (PPMV-1). The pathogenesis of PPMV-1 infections in poultry is largely obscure. It is known that PPMV-1 requires a series of passages in chickens before it becomes adapted to gallinaceous poultry.
Показать больше [+] Меньше [-]Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells Полный текст
2018
Terpiłowska Sylwia | Siwicka-Gieroba Dorota | Siwicki Andrzej Krzysztof
Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells Полный текст
2018
Terpiłowska Sylwia | Siwicka-Gieroba Dorota | Siwicki Andrzej Krzysztof
Introduction: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA.
Показать больше [+] Меньше [-]Cytotoxicity of iron (III), molybdenum (III), and their mixtures in BALB/3T3 and HepG2 cells Полный текст
2018
Terpiłowska, Sylwia | Siwicka-Gieroba, Dorota | Siwicki, Andrzej Krzysztof
Introduction: Iron and molybdenum are essential trace elements for cell metabolism. They are involved in maintaining proper functions of enzymes, cell proliferation, and metabolism of DNA. Material and Methods: BALB/3T3 and HepG2 cells were incubated with iron chloride or molybdenum trioxide at concentrations from 100 to 1,400 µM. The cells were also incubated in mixtures of iron chloride at 200 μM plus molybdenum trioxide at 1,000 μM or iron chloride at 1,000 μM plus molybdenum trioxide at 200 μM. Cell viability was determined with MTT reduction, LHD release, and NRU tests. Results: A decrease in cell viability was observed after incubating both cell lines with iron chloride or molybdenum trioxide. In cells incubated with mixtures of these trace elements, a decrease in cell viability was observed, assessed by all the used assays. Conclusions: Iron (III) and molybdenum (III) decrease cell viability in normal and cancer cells. A synergistic effect of the mixture of these elements was observed.
Показать больше [+] Меньше [-]Effects of natural antioxidants on the stability of omega-3 fatty acids in dog food Полный текст
2018
Glodde Florentina | Günal Mevlüt | Kinsel Mary E. | AbuGhazaleh Amer
Effects of natural antioxidants on the stability of omega-3 fatty acids in dog food Полный текст
2018
Glodde Florentina | Günal Mevlüt | Kinsel Mary E. | AbuGhazaleh Amer
The efficiency of five natural antioxidants (curcumin, cranberry, pomegranate, grape seed extract (GSE), and açai berry) in reducing lipid oxidation in dog food was compared to that of the synthetic antioxidant butylated hydroxyanisole (BHA).
Показать больше [+] Меньше [-]Effects of natural antioxidants on the stability of omega-3 fatty acids in dog food Полный текст
2018
Glodde, Florentina | Günal, Mevlüt | Kinsel, Mary E. | AbuGhazaleh, Amer
The efficiency of five natural antioxidants (curcumin, cranberry, pomegranate, grape seed extract (GSE), and açai berry) in reducing lipid oxidation in dog food was compared to that of the synthetic antioxidant butylated hydroxyanisole (BHA). In two different experiments content parameters were measured after 12 days of storage at 55°C. In experiment one, the natural antioxidants were added at 0.2% and BHA at 0.02% of the food (DM basis), and samples were analysed for thiobarbituric acid-reactive substances (TBARS). In experiment two, the effects of GSE and curcumin at two admixture proportions (0.1% and 0.2% of food DM) on omega-3 fatty acid (FA) content were evaluated. TBARS values were lower than the control (P < 0.01) for curcumin, cranberry, pomegranate, and GSE but not for the açai berry (P > 0.05). By day 12, although there were no significant differences (P > 0.05) between the two curcumin treatments, they preserved higher concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (P < 0.05) than the BHA and control treatments. The addition of GSE or BHA to dog food held (P < 0.05) the concentrations of EPA higher than the control. The concentrations of EPA and DHA for the 0.2% GSE treatment were greater (P < 0.05) than the 0.1% GSE treatment. Grape seed extract at 0.2% lost less (P < 0.05) EPA concentration than BHA. The present results showed that, except for açai berry, the tested natural antioxidants could be used as a substitute for BHA in dog food.
Показать больше [+] Меньше [-]Control of residues of thyreostats in slaughter animals in Poland in 2011–2017 Полный текст
2018
Woźniak Barbara | Matraszek-Żuchowska Iwona | Sielska Katarzyna | Witek Sebastian | Posyniak Andrzej | Niemczuk Krzysztof | Żmudzki Jan
Control of residues of thyreostats in slaughter animals in Poland in 2011–2017 Полный текст
2018
Woźniak Barbara | Matraszek-Żuchowska Iwona | Sielska Katarzyna | Witek Sebastian | Posyniak Andrzej | Niemczuk Krzysztof | Żmudzki Jan
Introduction: In the European Union, the use of thyreostatic drugs for fattening slaughter animals has been banned since 1981 under Council Directive 81/602/EEC. For protection of consumer health against unwanted residues and in compliance with Directive 96/23, each EU country must monitor thyreostats in samples of animal origin. This paper presents the results of research on thyreostatic residues carried out in Poland in 2011–2017.
Показать больше [+] Меньше [-]Control of residues of thyreostats in slaughter animals in Poland in 2011–2017 Полный текст
2018
Woźniak-Sobczak, Barbara | Matraszek-Żuchowska, Iwona | Sielska, Katarzyna | Witek, Sebastian | Posyniak, Andrzej | Niemczuk, Krzysztof | Żmudzki, Jan
Introduction: In the European Union, the use of thyreostatic drugs for fattening slaughter animals has been banned since 1981 under Council Directive 81/602/EEC. For protection of consumer health against unwanted residues and in compliance with Directive 96/23, each EU country must monitor thyreostats in samples of animal origin. This paper presents the results of research on thyreostatic residues carried out in Poland in 2011–2017. Material and Methods: The material for testing was urine (n = 3,491), drinking water (n = 127), and muscle samples (n = 349) officially collected by Veterinary Sanitary Inspectors in slaughterhouses and farms throughout the country in accordance with the national residue control plan. The samples were examined for the presence of tapazole, thiouracil, methylthiouracil, propylthiouracil, and phenylthiouracil using liquid chromatography tandem mass spectrometry through an accredited method. Results: In four bovine and three porcine urine samples, the permissible thiouracil concentration was exceeded. In one sample of porcine urine, methyl- and propylthiouracil were found. The presence of thiouracil and its derivatives in urine samples is most likely due to feeding animals diet containing cruciferous plants. Conclusions: The results of research indicate that thyreostats are not used for anabolic purposes in slaughter animals in Poland.
Показать больше [+] Меньше [-]Phenotypic and genotypic examination of antimicrobial resistance in thermophilic Campylobacter species isolated from poultry in Turkey Полный текст
2018
Adiguzel Mehmet Cemal | Sigirci Belgi Diren | Celik Baran | Kahraman Beren Basaran | Metiner Kemal | Ikiz Serkan | Bagcigil A. Funda | Ak Seyyal | Ozgur N. Yakut
Introduction: The study aimed to isolate thermophilic Campylobacter from chickens raised three rearing methods, determine its antimicrobial susceptibilities, and examine resistance-related genes by PCR.
Показать больше [+] Меньше [-]Genotoxicity and mutagenicity of inosine pranobex Полный текст
2018
Tobólska Sylwia | Terpiłowska Sylwia | Jaroszewski Jerzy | Siwicki Andrzej Krzysztof
Inosine pranobex (Methisoprinol, ISO, Isoprinosine) is an immuno-modulatory antiviral drug that has been licensed since 1971 in several countries worldwide. In humans, the drug is approved for the treatment of viral infections, and it might also have therapeutic use in animals. The aims of the presented work were to investigate the genotoxicity of inosine pranobex on BALB/3T3 clone A1 and HepG2 cell lines and to elucidate its mutagenicity using the Ames test.
Показать больше [+] Меньше [-]Use of a new LC-MS method for the determination of pyrrolizidine alkaloids in feeds Полный текст
2018
Kowalczyk Ewelina | Kwiatek Krzysztof
Use of a new LC-MS method for the determination of pyrrolizidine alkaloids in feeds Полный текст
2018
Kowalczyk Ewelina | Kwiatek Krzysztof
Pyrrolizidine alkaloids (PAs) are secondary metabolites produced by many plant species. Due to their toxicity PAs can pose a risk to human and animal health. To detect the toxic compounds in feed materials a sensitive method based on liquid chromatography coupled with mass spectrometry has been developed.
Показать больше [+] Меньше [-]Use of a new LC-MS method for the determination of pyrrolizidine alkaloids in feeds Полный текст
2018
Kowalczyk, Ewelina | Kwiatek, Krzysztof
Pyrrolizidine alkaloids (PAs) are secondary metabolites produced by many plant species. Due to their toxicity PAs can pose a risk to human and animal health. To detect the toxic compounds in feed materials a sensitive method based on liquid chromatography coupled with mass spectrometry has been developed. PAs were extracted with sulphuric acid and purified with cation exchange cartridges. A newly developed solvent mixture consisting of ethyl acetate, methanol, acetonitrile, ammonia, and triethylamine was used to wash alkaloids from the cartridges. After evaporation the residues were reconstituted in water and methanol mixture and subjected to LC-MS analysis. The developed method was validated according to SANTE/11945/2015 guidelines. The recovery was from 84.1% to 112.9%, the repeatability ranged from 3.0% to 13.6%, and the reproducibility was from 4.8% to 18.9%. A sensitive and selective method for determination of PAs in feed materials has been developed and validated. All evaluated validation parameters were in accordance with EU Reference Laboratories document no. SANTE/11945/2015. Almost 41% of the analysed feed samples were positive for the presence of at least one PA.
Показать больше [+] Меньше [-]Detection of Dinophysis species and associated okadaic acid in farmed shellfish: a two-year study from the western Mediterranean area Полный текст
2018
Bazzoni Anna Maria | Mudadu Alessandro G. | Lorenzoni Giuseppa | Soro Barbara | Bardino Nadia | Arras Igor | Sanna Giovanna | Vodret Bruna | Bazzardi Riccardo | Marongiu Edoardo | Virgilio Sebastiano
Diarrhoetic shellfish poisoning (DSP), an alimentary intoxication known to lead to intestinal symptoms, and caused by toxins produced by some dinoflagellates (including several Dinophysis), represents a serious threat to public health. The aim of this paper was to provide information about the occurrence and abundance of potentially toxic harmful algal species causing DSP, and the associated concentration of okadaic acid (OA) toxins. The departing assumption was that in the study area there was an increase in the presence both of Dinophysis species and OA and its derivates that could result in a risk to the health of seafood consumers.
Показать больше [+] Меньше [-]Ready-to-eat meat products as a source of Listeria monocytogenes Полный текст
2018
Kurpas Monika | Wieczorek Kinga | Osek Jacek
Ready-to-eat meat products as a source of Listeria monocytogenes Полный текст
2018
Kurpas Monika | Wieczorek Kinga | Osek Jacek
In 2015 in the European Union member states listeriosis caused 270 deaths. Food is the route of transmission in 99% of all human infection cases. Several studies from different countries have shown that the presence of Listeria monocytogenes in food can be as high as 58.3%. One of the most important ways to protect food from these microorganisms is to prevent the spread of the bacteria at processing plants at different stages of food production chain. The ability of L. monocytogenes to survive in extreme conditions and to form biofilms on various surfaces is a significant challenge for food safety. Removal of these bacteria from niches in processing plants is difficult and requires the use of sanitisers and precise equipment cleaning. The presence of L. monocytogenes in processing environment at slaughterhouses, deli meat factories or in retail may be a reason of cross-contamination. Proper hygienic systems applied by workers in food preparing places and knowledge about different routes of spreading of these bacteria may effectively decrease the risk of food contamination. Standardised legal regulations and control of meat product manufacture should be a fundamental way to protect food from L. monocytogenes contamination.
Показать больше [+] Меньше [-]Ready-to-eat meat products as a source of Listeria monocytogenes Полный текст
2018
Kurpas, Monika | Wieczorek, Kinga | Osek, Jacek
In 2015 in the European Union member states listeriosis caused 270 deaths. Food is the route of transmission in 99% of all human infection cases. Several studies from different countries have shown that the presence of Listeria monocytogenes in food can be as high as 58.3%. One of the most important ways to protect food from these microorganisms is to prevent the spread of the bacteria at processing plants at different stages of food production chain. The ability of L. monocytogenes to survive in extreme conditions and to form biofilms on various surfaces is a significant challenge for food safety. Removal of these bacteria from niches in processing plants is difficult and requires the use of sanitisers and precise equipment cleaning. The presence of L. monocytogenes in processing environment at slaughterhouses, deli meat factories or in retail may be a reason of cross-contamination. Proper hygienic systems applied by workers in food preparing places and knowledge about different routes of spreading of these bacteria may effectively decrease the risk of food contamination. Standardised legal regulations and control of meat product manufacture should be a fundamental way to protect food from L. monocytogenes contamination.
Показать больше [+] Меньше [-]Study on Toxoplasma gondii, Leptospira spp., Coxiella burnetii, and Echinococcus granulosus infection in veterinarians from Poland Полный текст
2018
Wójcik-Fatla Angelina | Sroka Jacek | Zając Violetta | Zwoliński Jacek | Sawczyn-Domańska Anna | Kloc Anna | Bilska-Zając Ewa | Chmura Robert | Dutkiewicz Jacek
Study on Toxoplasma gondii, Leptospira spp., Coxiella burnetii, and Echinococcus granulosus infection in veterinarians from Poland Полный текст
2018
Wójcik-Fatla Angelina | Sroka Jacek | Zając Violetta | Zwoliński Jacek | Sawczyn-Domańska Anna | Kloc Anna | Bilska-Zając Ewa | Chmura Robert | Dutkiewicz Jacek
Introduction: Exposure to zoonotic factors in veterinary practice is closely related to the nature of the work. The main aim of the study was to determine the risk of selected zoonotic infections among the occupational group of veterinarians in Poland.
Показать больше [+] Меньше [-]Study on Toxoplasma gondii, Leptospira spp., Coxiella burnetii, and Echinococcus granulosus infection in veterinarians from Poland Полный текст
2018
Wójcik-Fatla, Angelina | Sroka, Jacek | Zając, Violetta | Zwoliński, Jacek | Sawczyn-Domańska, Anna | Kloc, Anna | Bilska-Zając, Ewa | Chmura, Robert | Dutkiewicz, Jacek
Introduction: Exposure to zoonotic factors in veterinary practice is closely related to the nature of the work. The main aim of the study was to determine the risk of selected zoonotic infections among the occupational group of veterinarians in Poland. Material and Methods: Blood samples of 373 veterinarians (162 males and 211 females) from 12 provinces of Poland were collected by the venipuncture of a forearm for serological tests. Commercial immunoenzymatic tests (ELISA) were used for detection of specific IgG antibodies to Echinococcus granulosus, IgM and IgG to Leptospira spp., and IgM, IgA, and I and II phase IgG to Coxiella burnetii. Enzyme-linked fluorescence assays (ELFA) were used to detect IgM and IgG antibodies to Toxoplasma gondii. Results: Positive results were found in 209 (56.0%) veterinarians for at least one of the examined diseases. The overall proportion of participants found to have specific Toxoplasma gondii antibodies in the IgM and/or IgG assays amounted to 44.5%. The presence of Coxiella burnetii antibodies was found in 16 (4.3%) subjects, while Leptospira spp. antibodies were detected in 63 (16.9%) veterinarians. Among the 373 veterinarians examined, no Echinococcus granulosus antibodies were found. Conclusion: Results of the study seem to indicate a slightly elevated risk of Toxoplasma gondii infection and a moderate risk of infection with Leptospira spp. and Coxiella burnetii in veterinarians.
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