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Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing Полный текст
2018
Eftekhari Hadi | Jahandideh Alireza | Asghari Ahmad | Akbarzadeh Abolfazl | Hesaraki Saeed
Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing Полный текст
2018
Eftekhari Hadi | Jahandideh Alireza | Asghari Ahmad | Akbarzadeh Abolfazl | Hesaraki Saeed
In recent years, the use of bone scaffolds as bone tissue substitutes, especially the use of such as hydroxyapatite and tricalcium phosphate, has been very popular. Today, the use of modern engineering techniques and advances in nanotechnology have expanded the use of nanomaterials as bone scaffolds for bone tissue applications.
Показать больше [+] Меньше [-]Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing Полный текст
2018
Eftekhari, Hadi | Jahandideh, Alireza | Asghari, Ahmad | Akbarzadeh, Abolfazl | Hesaraki, Saeed
In recent years, the use of bone scaffolds as bone tissue substitutes, especially the use of such as hydroxyapatite and tricalcium phosphate, has been very popular. Today, the use of modern engineering techniques and advances in nanotechnology have expanded the use of nanomaterials as bone scaffolds for bone tissue applications. This study was performed on 60 adult male New Zealand rabbits divided into four experimental groups: the control group without any treatment, the second group receiving hydroxyapatite, the third group treated with β-tricalcium phosphate, and the fourth group receiving nanocomposite polycaprolactone (PCL) scaffold. In a surgical procedure, a defect 6 mm in diameter was made in a hind limb femur. Four indexes were used to assess histopathology, which were union index, spongiosa index, cortex index, and bone marrow. The results showed that nanocomposite PCL and control groups always had the respective highest and lowest values among all the groups at all time intervals. The histopathological assessment demonstrated that the quantity of newly formed lamellar bone in the nanocomposite PCL group was higher than in other groups. All these data suggest that PCL had positive effects on the bone healing process, which could have great potential in tissue engineering and clinical applications.
Показать больше [+] Меньше [-]Effect of TNF-α concentration on selected clinical parameters of swine after burns Полный текст
2018
Aleksiewicz Roman | Lutnicki Krzysztof | Likus Marta | Gątkiewicz Łukasz
Effect of TNF-α concentration on selected clinical parameters of swine after burns Полный текст
2018
Aleksiewicz Roman | Lutnicki Krzysztof | Likus Marta | Gątkiewicz Łukasz
The study aimed to observe TNF-α serum concentration as well as changes in respiration rate, body temperature, and pulse rate in burn victims during 84 h post burn.
Показать больше [+] Меньше [-]Effect of TNF-α concentration on selected clinical parameters of swine after burns Полный текст
2018
Aleksiewicz, Roman | Lutnicki, Krzysztof | Likus, Marta | Gątkiewicz, Łukasz
The study aimed to observe TNF-α serum concentration as well as changes in respiration rate, body temperature, and pulse rate in burn victims during 84 h post burn. A total of 30 healthy pigs were divided into two groups: A, the test group and N, the control group. The experimental group suffered burns to 30% of the body surface, and after infliction of the burns both groups were closely monitored. The biggest increase in TNF-α serum concentration in the test subjects occurred around the 6ᵗʰ h of the study, and the second biggest increase took place between 12ᵗʰ and 36ᵗʰ h. In the 36ᵗʰ h, TNF-α was 2.5 times more concentrated in serum in the test group than in the control group. In the test group, the biggest increase in respiration rate occurred up to the 6ᵗʰ h post burn, on average up to 29/min. In the 12ᵗʰ h post burn, the mean pulse rate in the test group was 133/min and dropped to the lowest value in the 72ⁿᵈ h of the experiment. A gradual increase in body temperature up to 41.72°C was observed up to the 30ᵗʰ h post burn and decreased to a significant value of 40.74°C by the 84ᵗʰ h of the study. In a period of a pronounced rise in TNF-α serum concentration, this parameter, pulse rate, and respiration rate are highly correlated and are also influenced by multiple inflammation forming factors.
Показать больше [+] Меньше [-]Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland Полный текст
2018
Pyz-Łukasik Renata | Paszkiewicz Waldemar
Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland Полный текст
2018
Pyz-Łukasik Renata | Paszkiewicz Waldemar
The purpose of this study was to determine the microbiological quality of food fish and its safety for consumers.
Показать больше [+] Меньше [-]Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland Полный текст
2018
Pyz-Łukasik, Renata | Paszkiewicz, Waldemar
The purpose of this study was to determine the microbiological quality of food fish and its safety for consumers. The study included 24 fish representing grass carp, bighead carp, Siberian sturgeon, and wels catfish. Specimens were collected in winter. Aerobic bacteria, psychrophilic, Enterobacteriaceae, Staphylococcus spp., and E. coli counts were made, and the presence of Salmonella spp., L. monocytogenes, S. aureus, and other coagulase-positive staphylococci was investigated. The microbiological analysis showed a similar level of aerobic, psychrophilic, and Staphylococcus spp. contamination of the four fish species. The Enterobacteriaceae count was higher in the muscles of grass carp and bighead carp than S. sturgeon and wels catfish. No pathogenic bacteria such as Salmonella spp., E. coli, L. monocytogenes, Staphylococcus aureus, or other coagulase positive staphylococci were found in samples of the examined fish species. The fresh fish examined in this study were of good microbiological quality and there was no health risk for consumers.
Показать больше [+] Меньше [-]Cell viability in normal fibroblasts and liver cancer cells after treatment with iron (III), nickel (II), and their mixture Полный текст
2018
Terpiłowska Sylwia | Siwicka-Gieroba Dorota | Siwicki Andrzej Krzysztof
Cell viability in normal fibroblasts and liver cancer cells after treatment with iron (III), nickel (II), and their mixture Полный текст
2018
Terpiłowska Sylwia | Siwicka-Gieroba Dorota | Siwicki Andrzej Krzysztof
Introduction: Nickel and iron are very commonly occurring metals. Nickel is used in industry, but nowadays it is also used in medical biomaterials. Iron is an element necessary for cell metabolism and is used in diet supplements and biomaterials, whence it may be released along with nickel.
Показать больше [+] Меньше [-]Cell viability in normal fibroblasts and liver cancer cells after treatment with iron (III), nickel (II), and their mixture Полный текст
2018
Terpiłowska, Sylwia | Siwicka-Gieroba, Dorota | Siwicki, Andrzej Krzysztof
Introduction: Nickel and iron are very commonly occurring metals. Nickel is used in industry, but nowadays it is also used in medical biomaterials. Iron is an element necessary for cell metabolism and is used in diet supplements and biomaterials, whence it may be released along with nickel. Material and Methods: BALB/3T3 and HepG2 cells were incubated with iron chloride or nickel chloride at concentrations ranging from 100 to 1,400 µM. The following mixtures were used: iron chloride 200 µM plus nickel chloride 1,000 µM, or iron chloride 1,000 µM plus nickel chloride 200 µM. The cell viability was determined with MTT, LHD, and NRU tests. Results: A decrease in cell viability was observed after incubating the BALB/3T3 and HepG2 cells with iron chloride or nickel chloride. A synergistic effect was observed after iron chloride 1,000 μM plus nickel chloride 200 μM treatment in all assays. Moreover, the same effect was observed in the pair iron chloride 200 μM plus nickel chloride 1,000 μM in the LDH and NRU assays. Conclusions: Iron (III) and nickel (II) decrease cell viability. Iron chloride at a concentration of 200 µM protects mitochondria from nickel chloride toxicity.
Показать больше [+] Меньше [-]Effect of extender supplementation with low-molecular-weight antioxidants on selected quality parameters of cryopreserved canine spermatozoa Полный текст
2018
Lecewicz Marek | Strzeżek Rafał | Kordan Władysław | Majewska Anna
Effect of extender supplementation with low-molecular-weight antioxidants on selected quality parameters of cryopreserved canine spermatozoa Полный текст
2018
Lecewicz Marek | Strzeżek Rafał | Kordan Władysław | Majewska Anna
Introduction The addition of low-molecular-weight antioxidants during the freezing process improves post-thaw sperm quality. The high antioxidant potential of cryopreserved semen could have a positive effect on the motility, viability, and energy status of sperm cells and their ability to bind to the zona pellucida of oocytes. The aim of the study was to determine the effects of different concentrations and combinations of vitamins E and C in a semen extender on selected quality parameters of frozen-thawed canine spermatozoa.
Показать больше [+] Меньше [-]Effect of extender supplementation with low-molecular-weight antioxidants on selected quality parameters of cryopreserved canine spermatozoa Полный текст
2018
Lecewicz, Marek | Strzeżek, Rafał | Kordan, Władysław | Majewska, Anna
Introduction The addition of low-molecular-weight antioxidants during the freezing process improves post-thaw sperm quality. The high antioxidant potential of cryopreserved semen could have a positive effect on the motility, viability, and energy status of sperm cells and their ability to bind to the zona pellucida of oocytes. The aim of the study was to determine the effects of different concentrations and combinations of vitamins E and C in a semen extender on selected quality parameters of frozen-thawed canine spermatozoa. Material and Methods The experimental material was the semen of four mixed-breed dogs. Sperm viability (motility, plasma membrane integrity, and mitochondrial function) was examined at 0, 60, and 120 min in semen samples supplemented with the extender and in the controls. Results Combined supplementation with vitamins C + E at a concentration of 200 + 200 μM /1 × 10⁹ spermatozoa had the most profound effect on total sperm motility, linear motility, and the percentage of spermatozoa with intact plasma membrane and active mitochondria. Conclusion The synergistic activity of vitamins E and C had a more beneficial influence on the quality of frozen–thawed sperm than these non-enzymatic antioxidants applied separately.
Показать больше [+] Меньше [-]Histopathological, immunohistochemical, and parasitological studies on pathogenesis of Coenurus cerebralis in sheep Полный текст
2018
Rahsan Yilmaz | Nihat Yumusak | Bestami Yilmaz | Adnan Ayan | Nuran Aysul
Histopathological, immunohistochemical, and parasitological studies on pathogenesis of Coenurus cerebralis in sheep Полный текст
2018
Rahsan Yilmaz | Nihat Yumusak | Bestami Yilmaz | Adnan Ayan | Nuran Aysul
This study consisted in histopathological and immunohistochemical examinations of the central nervous system of 15 sheep suspected of infection with Coenurus cerebralis. The sheep displayed compulsive circling and were submitted for necropsy in 2012–2016.
Показать больше [+] Меньше [-]Histopathological, immunohistochemical, and parasitological studies on pathogenesis of Coenurus cerebralis in sheep Полный текст
2018
Rahsan, Yilmaz | Nihat, Yumusak | Bestami, Yilmaz | Adnan, Ayan | Nuran, Aysul
This study consisted in histopathological and immunohistochemical examinations of the central nervous system of 15 sheep suspected of infection with Coenurus cerebralis. The sheep displayed compulsive circling and were submitted for necropsy in 2012–2016. Species identification was made on the basis of the PCR analysis and parasitological examination of the cysts. Coenurus cerebralis cysts were detected only in the cerebral tissue of 13 sheep and in the cerebral and cerebellar tissues of 2 animals. Out of the 33 parasite cysts, most (21.21%) were located in the right and left frontal lobes of the cerebrum. The largest cyst measured 6 × 5 cm and the smallest cyst was 2 × 2 cm in size. The highest and lowest numbers of scolices were 55 and 21, and the number of rostellar hooks ranged between 22 and 30. Histopathological examination revealed the presence of typical parasitic granulomatous inflammatory foci. Immunohistochemical staining showed that most common in the periphery of the parasite cysts were, in descending order by cell number, GFAP, CD163, CD3, and CD79α-positive cells. The study confirms the role of cellular defence mechanisms in the pathogenesis of Coenurus cerebralis infection in sheep.
Показать больше [+] Меньше [-]Complete genome sequences of three sub-genotype 2.1b isolates of classical swine fever virus in China Полный текст
2018
Liu Chunxiao | Li Mingliang | Yin Xingwang | Zhang Hongliang | Xiang Lirun | Zhai Hongyue | Wang Congcong | Kan Yunchao | Yao Lunguang | Tian Zhijun | Leng Chaoliang
Classical swine fever (CSF) has caused severe economic losses in pig production in many countries. Recent CSF outbreaks in China are mainly associated with sub-genotype 2.1 of CSF virus (CSFV). Although there is abundant information regarding 2.1 isolates, few data are available on whole-genome analysis.
Показать больше [+] Меньше [-]Relationship between the content of chlorinated hydrocarbons and fatty acid composition of milk fat Полный текст
2018
Pietrzak-Fiećko Renata
Relationship between the content of chlorinated hydrocarbons and fatty acid composition of milk fat Полный текст
2018
Pietrzak-Fiećko Renata
Reports that the presence of persistent organic pollutants in fat may affect fatty acid metabolism prompted this research aiming to study the relationship between the contents of γ-HCH and DDT, DDE, DDD, and ΣDDT, and fatty acid composition of milk fat.
Показать больше [+] Меньше [-]Relationship between the content of chlorinated hydrocarbons and fatty acid composition of milk fat Полный текст
2018
Pietrzak-Fiećko, Renata
Reports that the presence of persistent organic pollutants in fat may affect fatty acid metabolism prompted this research aiming to study the relationship between the contents of γ-HCH and DDT, DDE, DDD, and ΣDDT, and fatty acid composition of milk fat. The material consisted of 50 samples of cow and mare milk, collected in 2015. Ludwicki’s and the Röse-Gottlieb and IDF Standard methods were used to prepare the samples. Statistical analyses were conducted using Statistica 12.0. There was a negative correlation between the content of γ-HCH and C16:1, C17:1, C18:1c9, C18:1c9c12, and ΣMUFA in cow milk fat and C13:0, C14:0, and C10:1 in mare milk fat. A positive correlation was observed between γ-HCH and C6:0 to C12:0, C14:0, C18:1t16, and ΣSFA in cow milk fat, and between this compound and C14:0iso, C16:1, C17:1, C18:1c9,11, and ΣMUFA in mare milk fat. A negative correlation between the contents of ΣDDT and C16:1, C17:1, C18:1c9,11,13 and ΣMUFA in cow milk fat and C16:0iso, C17:0, and C18:3 in mare milk fat was noted. A positive correlation was found between the contents of ΣDDT and saturated and polyunsaturated fatty acids and ΣSFA and ΣPUFA in cow milk fat, and C18:2c9c12 in mare milk fat. The correlation between the content of selected organochlorine compounds and the composition of fatty acids in cow and mare milk fat indicates the strong influence of these environmental pollutants on the nutritional value of milk fat.
Показать больше [+] Меньше [-]Results of a 16-week safety assurance study with rats fed genetically modified Bt maize: effect on growth and health parameters Полный текст
2018
Szymczyk Beata | Szczurek Witold | Świątkiewicz Sylwester | Kwiatek Krzysztof | Sieradzki Zbigniew | Mazur Małgorzata | Bednarek Dariusz | Reichert Michał
Introduction: The influence of feeding genetically modified MON 810 hybrid maize on the growth and haematological and biochemical indices of rats was tested.
Показать больше [+] Меньше [-]Trichinella spiralis: impact on the expression of Toll-like receptor 4 (TLR4) gene during the intestinal phase of experimental trichinellosis Полный текст
2018
Wojtkowiak-Giera Agnieszka | Wandurska-Nowak Elżbieta | Derda Monika | Jankowska Wiesława | Jagodziński Paweł P. | Hadaś Edward
Trichinella spiralis: impact on the expression of Toll-like receptor 4 (TLR4) gene during the intestinal phase of experimental trichinellosis Полный текст
2018
Wojtkowiak-Giera Agnieszka | Wandurska-Nowak Elżbieta | Derda Monika | Jankowska Wiesława | Jagodziński Paweł P. | Hadaś Edward
Introduction: Toll-like receptors (TLRs) play a key role in the rapid activation of the innate immune response to a variety of pathogens. The aim of this study was to evaluate the effect of Trichinella spiralis infection on the level of expression of the tlr4 gene in mouse intestines during the intestinal phase of experimental trichinellosis.
Показать больше [+] Меньше [-]Trichinella spiralis: impact on the expression of Toll-like receptor 4 (TLR4) gene during the intestinal phase of experimental trichinellosis Полный текст
2018
Wojtkowiak-Giera, Agnieszka | Wandurska-Nowak, Elżbieta | Derda, Monika | Jankowska, Wiesława | Jagodziński, Paweł P. | Hadas, Edward
Introduction: Toll-like receptors (TLRs) play a key role in the rapid activation of the innate immune response to a variety of pathogens. The aim of this study was to evaluate the effect of Trichinella spiralis infection on the level of expression of the tlr4 gene in mouse intestines during the intestinal phase of experimental trichinellosis. Material and Methods: The experimental material consisted of the small and large intestines of BALB/c mice infected with Trichinella spiralis sampled at 4, 8, and 16 days post infection (dpi). Results: A statistically significant increase was demonstrated in the tlr4 mRNA level isolated from the infected mice jejunum at 4, 8, and 16 dpi over the uninfected control. Moreover, at 4, 8, and 16 dpi in the jejunum of infected mice, a strong positive reaction for the presence of TLR4 protein compared with that of uninfected mice was observed. Conclusion: Infection with T. spiralis changes the expression of the tlr4 gene in the small intestine of the mouse host.
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