Objective: The research was accomplished to appraise the productive and reproductive physiology of repeat breeder (RB) cows and compare to normal cyclic (NC) cows.Methodology: A total of 366 RB cows were surveyed from 1859 crossbred and indigenous cows using a questionnaire upon positive sampling of RB syndrome in dairy farms throughout the selected areas of Bangladesh. Out of 366 RB cows, 170 were randomly selected, which were at day 0 of estrous cycle. To compare the physiological characteristics, 170 NC cows at day 0 of estrous cycle were also selected randomly.Results: Results showed that age, BCS, and parity were higher in RB cows than NC cows. Survey on productive characteristics illustrated that milk production (P<0.05) and lactation length (P<0.01) was significantly higher in RB cows compared with NC cows. The study on reproductive features exposed that age at puberty and the first calving age was significantly (P<0.01) lower but the number of service, gestation length, calving interval, and voluntary waiting period were significantly (P<0.01) higher in RB cows than that of NC cows. Ovarian physiological characteristics revealed that estrus duration was significantly (P<0.05) lower in RB cows in comparison with NC cows. In addition, the largest follicular diameter was significantly (P<0.01) lower but vaginal electrical resistance (VER) was significantly (P<0.01) higher in RB cows compared with NC cows at day 0 of the estrous cycle.Conclusion: Results indicate hurly-burly characteristics of ovarian physiology in RB cows. This study could help veterinarians and researchers to define various risk factors associated with RB cows. http://doi.org/10.5455/javar.2018.e283

Objective: The research was accomplished to appraise the productive and reproductive physiology of repeat breeder (RB) cows and compare to normal cyclic (NC) cows. Methodology: A total of 366 RB cows were surveyed from 1859 crossbred and indigenous cows using a questionnaire upon positive sampling of RB syndrome in dairy farms throughout the selected areas of Bangladesh. Out of 366 RB cows, 170 were randomly selected, which were at day 0 of estrous cycle. To compare the physiological characteristics, 170 NC cows at day 0 of estrous cycle were also selected randomly. Results: Results showed that age, BCS, and parity were higher in RB cows than NC cows. Survey on productive characteristics illustrated that milk production (P [J Adv Vet Anim Res 2018; 5(3.000): 324-331]

Objective: The objectives of our study were to estimate the effect of some non-genetic factors on production and reproduction traits of Egyptian buffaloes and to estimate the breeding values for these traits. Materials and Methods: Data from 965 lactation records of 305 Egyptian buffalo cows mated by 73 sires that were raised at Mahallet Mousa Experimental farms of Animal Production Research Institute were collected. Dairy records covered the period from 2001 to 2015. The data were arranged and statistically analyzed using SAS version 9.1.3 to study the effect of non-genetic factors and the MTDFREMAL program was included in estimating the expected breeding values. Results: Our results showed that buffalo cows that calved in winter season recorded the highest and significant total milk yield (TMY) and daily milk yield (DMY) traits and the best reproductive performance was indicated by the lower number of services per conception and the shortest calving interval (CI) period. Moreover, age at first calving and dry period (DP) showed a highly significant effect on the TMY and DMY. A significant effect for the level of milk production on days open (DO) period was recorded in our study. The range of sires EBV was 540 kg, 295.2 days, 113.9 days, 2.8 months, and 71.1 days for TMY, LL, DO, CI, and DP, respectively. Conclusion: Buffalo’s breeders must consider the season of calving, parity, and DP in management program because they greatly affect the farm productivity and profitability and they must select animals with higher breeding values to be the parent of the next generation. http://doi.org/10.5455/javar.2018.e287

Objective: The objectives of our study were to estimate the effect of some non-genetic factors on production and reproduction traits of Egyptian buffaloes and to estimate the breeding values for these traits. Materials and Methods: Data from 965 lactation records of 305 Egyptian buffalo cows mated by 73 sires that were raised at Mahallet Mousa Experimental farms of Animal Production Research Institute were collected. Dairy records covered the period from 2001 to 2015. The data were arranged and statistically analyzed using SAS version 9.1.3 to study the effect of non-genetic factors and the MTDFREMAL program was included in estimating the expected breeding values. Results: Our results showed that buffalo cows that calved in winter season recorded the highest and significant total milk yield (TMY) and daily milk yield (DMY) traits and the best reproductive performance was indicated by the lower number of services per conception and the shortest calving interval (CI) period. Moreover, age at first calving and dry period (DP) showed a highly significant effect on the TMY and DMY. A significant effect for the level of milk production on days open (DO) period was recorded in our study. The range of sires EBV was 540 kg, 295.2 days, 113.9 days, 2.8 months, and 71.1 days for TMY, LL, DO, CI, and DP, respectively. Conclusion: Buffalos breeders must consider the season of calving, parity, and DP in management program because they greatly affect the farm productivity and profitability and they must select animals with higher breeding values to be the parent of the next generation. [J Adv Vet Anim Res 2018; 5(4.000): 374-380]

Objective: Sarcocystis spp. are common parasites and in terms of economics and pathogenicity in domestic animals is important. The purpose of this work was to define the rate of contamination of slaughtered carcasses of cattle to Sarcocystis using digestive and histopathological methods in southeast Iran. Materials and Methods: In this descriptive laboratory study for 1 year, 500 carcasses were examined and isolated bradyzoites of Sarcocystis with the digest method. Also, tissue samples from the esophagus and diaphragm were considered for pathologic studies and stained with hematoxylin and eosin of sections of histopathological. Results: The results showed that the highest contaminations were in imported male animals aged 2–3 years old in the spring. There was a significant difference (p < 0.05) in the prevalence rate with the sex and race of cattle but no significant difference (p > 0.05) in the prevalence rate with age and season. Conclusion: Infection with Sarcocystis is common in oxen in this region. The imported cattle are more infected. It seems that racing and the environmental condition affect the prevalence of Sarcocystosis. http://doi.org/10.5455/javar.2018.e288

Objective: Sarcocystis spp. are common parasites and in terms of economics and pathogenicity in domestic animals is important. The purpose of this work was to define the rate of contamination of slaughtered carcasses of cattle to Sarcocystis using digestive and histopathological methods in southeast Iran. Material and Methods: In this descriptive laboratory study for 1 year, 500 carcasses were examined and isolated bradyzoites of Sarcocystis with the digest method. Also, tissue samples from the esophagus and diaphragm were considered for pathologic studies and stained with hematoxylin and eosin of sections of histopathological. Results: The results showed that the highest contaminations were in imported male animals aged 23 years old in the spring. There was a significant difference (p < 0.05) in the prevalence rate with the sex and race of cattle but no significant difference (p > 0.05) in the prevalence rate with age and season. Conclusion: Infection with Sarcocystis is common in oxen in this region. The imported cattle are more infected. It seems that racing and the environmental condition affect the prevalence of Sarcocystosis. [J Adv Vet Anim Res 2018; 5(4.000): 381-387]

Objective: The present study was conducted to isolate and identify the Acanthamoeba species from various water sources such as drinking water, tap water, swimming pool, and other recreational water. Materials and methods: During the study period, 57 water samples were collected from various sources such as tap water, drinking water, swimming pool, and recreational water. All samples were processed and cultured on non-nutrient agar medium (NNA) with Escherichia coli overlay for the isolation of Acanthamoeba species. Organism identified based on the microscopic morphology of cyst and trophozoites forms. The pathogenicity of Acanthamoeba was analyzed by thermotolerance and osmotolerance assays. Results: Acanthamoeba were detected in 10 out of 57 (17.5%) examined water samples. The high percentage of positivity was observed in bore well water stored in tanks (37.5%) and in recreational water samples (26.7%). All processed drinking water samples were free from Acanthamoeba. Based on pathogenicity test assays, four (40%) were pathogenic and three (30%) were non-pathogenic. The observed frequency of Acanthamoeba spp. was compared with available literature worldwide. Conclusion: This study is the first report showing the distribution of Acanthamoeba in various water sources in the central region of Saudi Arabia and confirms that the high percentage presence of pathogenic strains in recreational water could threat contact lens wearers. Further research works are required to identify the prevalence of pathogenic Acanthamoeba from various water sources in Saudi Arabia. http://doi.org/10.5455/javar.2018.e296

Objective: The present study was conducted to isolate and identify the Acanthamoeba species from various water sources such as drinking water, tap water, swimming pool, and other recreational water. Materials and methods: During the study period, 57 water samples were collected from various sources such as tap water, drinking water, swimming pool, and recreational water. All samples were processed and cultured on non-nutrient agar medium (NNA) with Escherichia coli overlay for the isolation of Acanthamoeba species. Organism identified based on the microscopic morphology of cyst and trophozoites forms. The pathogenicity of Acanthamoeba was analyzed by thermotolerance and osmotolerance assays. Results: Acanthamoeba were detected in 10 out of 57 (17.5%) examined water samples. The high percentage of positivity was observed in bore well water stored in tanks (37.5%) and in recreational water samples (26.7%). All processed drinking water samples were free from Acanthamoeba. Based on pathogenicity test assays, four (40%) were pathogenic and three (30%) were non-pathogenic. The observed frequency of Acanthamoeba spp. was compared with available literature worldwide. Conclusion: This study is the first report showing the distribution of Acanthamoeba in various water sources in the central region of Saudi Arabia and confirms that the high percentage presence of pathogenic strains in recreational water could threat contact lens wearers. Further research works are required to identify the prevalence of pathogenic Acanthamoeba from various water sources in Saudi Arabia. [J Adv Vet Anim Res 2018; 5(4.000): 439-444]

Objective: This study was aimed at studying efficacy of medical management of corneal opacity in an Asian elephant (Elephas maximus).Material and methods: A 42 years old male Asian elephant was brought to the Teaching Veterinary Hospital (TVH) at Chittagong Veterinary and Animal Sciences University (CVASU) with a history of chronic lacrimation and impaired vision. On clinical examination, the animal was found apparently healthy. Opthalmological examination tentatively revealed the condition as corneal opacity. The left eye was much more affected as compared to the right one. Medical management was instituted with topical administration of ciprofloxacin, dexamethasone, subconjunctival prednisolone and dexamethasone along with intramuscular ketoprofen (at 1 mg/Kg bwt) and vitamin A (at 5000 IU/Kg bwt).Results: The “mahout” (elepenat caretaker) of the elephant was kept in close contact over cell phone to follow up the progress of the condition. Clinical examination after 19 days revealed complete recovery of the cornel opacity. There was no sign of lacrimation and the animal regained its normal vision.Conclusion: The treatment protocol successfully eliminated the discomfort along with corneal opacity and lacrimation in an Asian elephant.http://doi.org/10.5455/javar.2018.e244

Objective: This study was aimed at studying efficacy of medical management of corneal opacity in an Asian elephant (Elephas maximus). Material and methods: A 42 years old male Asian elephant was brought to the Teaching Veterinary Hospital (TVH) at Chittagong Veterinary and Animal Sciences University (CVASU) with a history of chronic lacrimation and impaired vision. On clinical examination, the animal was found apparently healthy. Opthalmological examination tentatively revealed the condition as corneal opacity. The left eye was much more affected as compared to the right one. Medical management was instituted with topical administration of ciprofloxacin, dexamethasone, subconjunctival prednisolone and dexamethasone along with intramuscular ketoprofen (at 1 mg/Kg bwt) and vitamin A (at 5000 IU/Kg bwt). Results: The mahout (elepenat caretaker) of the elephant was kept in close contact over cell phone to follow up the progress of the condition. Clinical examination after 19 days revealed complete recovery of the cornel opacity. There was no sign of lacrimation and the animal regained its normal vision. Conclusion: The treatment protocol successfully eliminated the discomfort along with corneal opacity and lacrimation in an Asian elephant. [J Adv Vet Anim Res 2018; 5(1.000): 98-100]