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Cytological evaluation of the influence of high and low doses of bisphenol A on an erythroblastic cell line of porcine bone marrow Полный текст
2018
Snarska, Anna | Wysocka, Dominika | Rytel, Liliana | Makowska, Krystyna | Gonkowski, Sławomir
Cytological evaluation of the influence of high and low doses of bisphenol A on an erythroblastic cell line of porcine bone marrow Полный текст
2018
Snarska, Anna | Wysocka, Dominika | Rytel, Liliana | Makowska, Krystyna | Gonkowski, Sławomir
Introduction: Bisphenol A (BPA) is a substance widely used in industry for the production of polycarbonates and epoxy resins used in packaging and containers for beverages, contact lenses, compact discs (CDs), window panes, and many other elements. This compound belongs to the group of polyphenols and xenoestrogens commonly found in the human environment. What we know about BPA is still insufficient to enable us to protect our health against its adverse effects, and current knowledge of the influence of BPA on erythroblastic cell lines in bone marrow is rather fragmentary. The aim of the experiment was to assess the effect of two doses of BPA (0.05 mg/kg and 0.5 mg/kg b.w. per day) on myeloid haematopoiesis. Material and Methods: During this experiment, the number of all types of cells in the erythroblastic cell line was evaluated in porcine bone marrow before and after BPA administration. Results: The obtained results clearly indicate changes in haematopoietic activity of the bone marrow, which was demonstrated by a decrease in erythroblastic cell line production in both experimental groups. The haematological effects of the bone marrow changes were anaemia, caused by a number of erythrocytes which was depressed due to their immaturity, and a significant decrease in mean cellular volume in both groups. Conclusion: The harmful effect of high and low doses of BPA on haematopoietic processes was proved.
Показать больше [+] Меньше [-]Cytological evaluation of the influence of high and low doses of bisphenol A on an erythroblastic cell line of porcine bone marrow Полный текст
2018
Snarska Anna | Wysocka Dominika | Rytel Liliana | Makowska Krystyna | Gonkowski Sławomir
Introduction: Bisphenol A (BPA) is a substance widely used in industry for the production of polycarbonates and epoxy resins used in packaging and containers for beverages, contact lenses, compact discs (CDs), window panes, and many other elements. This compound belongs to the group of polyphenols and xenoestrogens commonly found in the human environment. What we know about BPA is still insufficient to enable us to protect our health against its adverse effects, and current knowledge of the influence of BPA on erythroblastic cell lines in bone marrow is rather fragmentary. The aim of the experiment was to assess the effect of two doses of BPA (0.05 mg/kg and 0.5 mg/kg b.w. per day) on myeloid haematopoiesis.
Показать больше [+] Меньше [-]Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs Полный текст
2018
Bai, Ge | Li, Hui | Ge, Yansong | Zhang, Qianzhen | Zhang, Jiantao | Chen, Mingzi | Liu, Tao | Wang, Hongbin
Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs Полный текст
2018
Bai, Ge | Li, Hui | Ge, Yansong | Zhang, Qianzhen | Zhang, Jiantao | Chen, Mingzi | Liu, Tao | Wang, Hongbin
The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury. Eighteen miniature pigs were randomly divided into three groups: a sham operated group (sham group, laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), and a hydrogen-rich saline intervention group (IRI + HRS group). Samples of hepatic tissue and serum were collected at the time of reperfusion and then 3 h, 1 d, and 3 d post reperfusion. Liver function, oxidative stress, autophagy-related mRNA genes, and protein expression were evaluated. Changes in cell and tissue ultrastructure were examined by transmission electron microscopy. Compared with the sham group, the level of autophagy of hepatocytes increased in the IRI and IRI + HRS groups, corresponding to high oxidative stress and severe liver function injury. Liver function, antioxidant content, autophagy levels, and liver injury were improved after intervention with HRS in the IRI + HRS group compared with the IRI group. Intervention with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy.
Показать больше [+] Меньше [-]Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs Полный текст
2018
Bai Ge | Li Hui | Ge Yansong | Zhang Qianzhen | Zhang Jiantao | Chen Mingzi | Liu Tao | Wang Hongbin
The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury.
Показать больше [+] Меньше [-]New insight of apparently healthy animals as a potential reservoir for Clostridium perfringens: a public health implication Полный текст
2018
Hamza, Dalia | Dorgham, Sohad M. | Elhariri, Mahmoud | Elhelw, Rehab | Ismael, Elshaimaa
New insight of apparently healthy animals as a potential reservoir for Clostridium perfringens: a public health implication Полный текст
2018
Hamza, Dalia | Dorgham, Sohad M. | Elhariri, Mahmoud | Elhelw, Rehab | Ismael, Elshaimaa
Introduction: Clostridium perfringens is commonly found in the gastrointestinal tract of animals and humans and continues to cause one of the most prevalent foodborne diseases in man. Material and Methods: A total of 355 samples were examined for the occurrence of C. perfringens: rectal swabs from cattle, sheep, and goats, fresh stool samples from diarrhoea sufferers having been in contact with these animals, irrigation water and soil samples from the husbandry sites, and preharvesting fresh produce from farms irrigated with the sampled water. All samples were collected from Cairo and Giza governorates, Egypt. PCR analysis was carried out with positive isolates using the α-toxin gene. Sequence analysis of the gene of C. perfringens isolates was performed using the neighbour-joining approach. Bootstrap analysis was executed with 1,000 resamplings. Results: 174 C. perfringens strains were isolated with a 49.01% prevalence. The highest prevalence of C. perfringens in apparently healthy animals was found in sheep (65.45%) followed by goats (58%), buffaloes (55%), and cattle (47.1%). Its prevalence in humans being in contact with these animals was 47.5%. The bacterium’s isolation from the soil and irrigation water was achieved in 40% and 31.7% of samples, respectively, posing a risk, particularly when the water and soil contact food in the field, shown by the fresh produce isolation of 40%. A significant relationship between the prevalence of C. perfringens in animal and environmental samples was identified (P < 0.05). A significant relationship was identified neither between animal species and C. perfringens prevalence, nor between the environmental source and C. perfringens prevalence (P > 0.05). All isolates were positive for the α-toxin gene by PCR. The sequence analysis and the phylogenetic relationship of the α-toxin genes from different samples revealed that C. perfringens from faeces of apparently healthy cattle, buffaloes, sheep, and goats is a significant threat in places where it can contaminate the soil and water. In addition, the sequence of C. perfringens from humans suffering from diarrhoea was found in the same cluster with the sequence from cows, goats, and sheep. Conclusion: The role of apparently healthy animals in transmitting C. perfringens to humans, either through being in direct or indirect contact via water or soil in the cultivation of vegetables and fruits, was demonstrated.
Показать больше [+] Меньше [-]New insight of apparently healthy animals as a potential reservoir for Clostridium perfringens: a public health implication Полный текст
2018
Hamza Dalia | Dorgham Sohad M. | Elhariri Mahmoud | Elhelw Rehab | Ismael Elshaimaa
Introduction:Clostridium perfringens is commonly found in the gastrointestinal tract of animals and humans and continues to cause one of the most prevalent foodborne diseases in man.
Показать больше [+] Меньше [-]Immune-enhancing activity of potential probiotic strains of Lactobacillus plantarum in the common carp (Cyprinus carpio) fingerling Полный текст
2018
Kazuń Barbara | Małaczewska Joanna | Kazuń Krzysztof | Żylińska-Urban Joanna | Siwicki Andrzej K.
Immune-enhancing activity of potential probiotic strains of Lactobacillus plantarum in the common carp (Cyprinus carpio) fingerling Полный текст
2018
Kazuń Barbara | Małaczewska Joanna | Kazuń Krzysztof | Żylińska-Urban Joanna | Siwicki Andrzej K.
Introduction: Immune-potentiating functions of Lactobacillus plantarum strains in the common carp were evaluated.
Показать больше [+] Меньше [-]Immune-enhancing activity of potential probiotic strains of Lactobacillus plantarum in the common carp (Cyprinus carpio) fingerling Полный текст
2018
Kazuń, Barbara | Małaczewska, Joanna | Kazuń, Krzysztof | Żylińska-Urban, Joanna | Siwicki, Andrzej K.
Introduction: Immune-potentiating functions of Lactobacillus plantarum strains in the common carp were evaluated. Material and Methods: Fourteen days of feeding fish dry diet supplemented with the bacteria provided parameters of nonspecific humoral immunity (lysozyme, ceruloplasmin, γ-globulin, total protein levels, and serum bactericidal activity) and cellular immunity (pinocytosis, respiratory burst activity, and potential killing activity of organ phagocytes), as well as the proliferative response of organ lymphocytes stimulated with mitogens. The resistance of fish to infection with Aeromonas hydrophila was also determined. Results: Dietary supplementation with L. plantarum had a substantial influence on the activity of organ phagocytes, especially the potential killing activity of head kidney cells. A significant increase in the proliferative activity of LPS-stimulated B lymphocytes and in the levels of γ-globulins and total protein was observed. The supplemented diet conveyed higher resistance than the control diet as the cumulative fish mortalities after infection with A. hydrophila were 65% and 85%, respectively. Conclusion: The results indicate that dietary supplementation with L. plantarum stimulates the antibacterial resistance of common carp and may reinforce defence against bacterial infections, but further studies need to be conducted.
Показать больше [+] Меньше [-]Contemporary threats of bacterial infections in freshwater fish Полный текст
2018
Pękala-Safińska Agnieszka
Contemporary threats of bacterial infections in freshwater fish Полный текст
2018
Pękala-Safińska Agnieszka
Changes occurring in freshwater ecosystems seem to be fundamental in the development of all microorganisms, including those pathogenic to fish. This has been especially evident in recent years during which dynamic variations in bacterial fish pathology have been observed. Gram-negative bacteria commonly known to be pathogenic to fish, like Aeromonas spp., Flavobacterium spp., Pseudomonas spp., and Shewanella putrefaciens are replaced by other species, which until now have not been known to be virulent or even conditionally pathogenic to fish. Nowadays, among these other species Acinetobacter spp., Plesiomonas shigelloides, Sphingomonas paucimobilis, and Stenotrophomonas maltophilia are the most frequently isolated from fish exhibiting clinical signs of disease. Two Gram-positive bacteria have become pathogens of particular importance in fish pathology in Poland: Lactococcus garviae and Streptococcus iniae. In addition, infections caused by the Gram-positive bacterium Kocuria rhizophila have appeared in recent years. This bacterium has not been known until now to be pathogenic to fish. Therefore, this infection could be called an emergent disease.
Показать больше [+] Меньше [-]Contemporary threats of bacterial infections in freshwater fish Полный текст
2018
Pękala-Safińska, Agnieszka
Changes occurring in freshwater ecosystems seem to be fundamental in the development of all microorganisms, including those pathogenic to fish. This has been especially evident in recent years during which dynamic variations in bacterial fish pathology have been observed. Gram-negative bacteria commonly known to be pathogenic to fish, like Aeromonas spp., Flavobacterium spp., Pseudomonas spp., and Shewanella putrefaciens are replaced by other species, which until now have not been known to be virulent or even conditionally pathogenic to fish. Nowadays, among these other species Acinetobacter spp., Plesiomonas shigelloides, Sphingomonas paucimobilis, and Stenotrophomonas maltophilia are the most frequently isolated from fish exhibiting clinical signs of disease. Two Gram-positive bacteria have become pathogens of particular importance in fish pathology in Poland: Lactococcus garviae and Streptococcus iniae. In addition, infections caused by the Gram-positive bacterium Kocuria rhizophila have appeared in recent years. This bacterium has not been known until now to be pathogenic to fish. Therefore, this infection could be called an emergent disease.
Показать больше [+] Меньше [-]Ameliorative effects of Helianthus annuus against nephrotoxic, cardiac, and haematological disorders in alloxan-induced hyperglycaemia in albino rats Полный текст
2018
Onoja Samuel Okwudili | Udem Samuel Chukwuneke | Anaga Aruh Ottah
The study evaluated the ameliorative effects of Helianthus annuus leaf extract on nephrotoxicity, cardiac, and haematologic disorders in alloxan-induced hyperglycaemic rats.
Показать больше [+] Меньше [-]Histamine content in rennet ripening cheeses during storage at different temperatures and times Полный текст
2018
Madejska Anna | Michalski Mirosław | Pawul-Gruba Marzena | Osek Jacek
Histamine content in rennet ripening cheeses during storage at different temperatures and times Полный текст
2018
Madejska Anna | Michalski Mirosław | Pawul-Gruba Marzena | Osek Jacek
In recent years, there has been a great interest in biogenic amines such histamine, as they are associated with the quality and safety of some kinds of fermented foods. The aim of this study was to evaluate the effect of temperature and storage time on the content of histamine in cheeses.
Показать больше [+] Меньше [-]Histamine content in rennet ripening cheeses during storage at different temperatures and times Полный текст
2018
Madejska, Anna | Michalski, Mirosław | Pawul-Gruba, Marzena | Osek, Jacek
In recent years, there has been a great interest in biogenic amines such histamine, as they are associated with the quality and safety of some kinds of fermented foods. The aim of this study was to evaluate the effect of temperature and storage time on the content of histamine in cheeses. Samples of mould and hard cheeses were examined with RP-HPLC with an organic-aqueous mobile phase containing acidic buffer and chaotropic salt. The samples were stored either at 22 ± 2°C for 42 days (mould and hard cheeses) or at 4 ± 2°C for 112 days (mould cheeses) and 133 days (hard cheeses). The mean total histamine content in cheeses stored at 22°C was higher than the content in those stored at 4°C, with the highest concentrations found in Gorgonzola Piccante cheese (730.47 mg/kg). Histamine concentration in some types of cheeses exceeded the toxic threshold dose, indicating that after long or inadequately cool storage they may not be safe for consumers. To protect cheeses from contamination with histamine-producing bacteria and to safeguard consumers from poisoning, factors conducive to this amine’s formation should be minimised during cheese processing. Suitable temperature and time during storage of cheeses are recommended to avoid the intoxication. Monitoring of this toxin in food is necessary to ensure safety of consumers.
Показать больше [+] Меньше [-]Simvastatin-induced changes in the leukocytic system of porcine bone marrow Полный текст
2018
Snarska Anna | Wysocka Dominika | Rytel Liliana | Gonkowski Sławomir | Pawelec Hanna | Sobiech Przemysław
Simvastatin-induced changes in the leukocytic system of porcine bone marrow Полный текст
2018
Snarska Anna | Wysocka Dominika | Rytel Liliana | Gonkowski Sławomir | Pawelec Hanna | Sobiech Przemysław
Simvastatin is a substance which is commonly used as a medicine to reduce cholesterol level. Unfortunately, it shows numerous side effects. Simvastatin affects various internal organs, and among other detriments to health may cause persistent muscle weakness, osteolytic processes, headaches, and rashes. Until now knowledge of the influence of simvastatin on bone marrow cells has been rather scant and fragmentary.
Показать больше [+] Меньше [-]Simvastatin-induced changes in the leukocytic system of porcine bone marrow Полный текст
2018
Snarska, Anna | Wysocka, Dominika | Rytel, Liliana | Gonkowski, Sławomir | Pawelec, Hanna | Sobiech, Przemysław
Simvastatin is a substance which is commonly used as a medicine to reduce cholesterol level. Unfortunately, it shows numerous side effects. Simvastatin affects various internal organs, and among other detriments to health may cause persistent muscle weakness, osteolytic processes, headaches, and rashes. Until now knowledge of the influence of simvastatin on bone marrow cells has been rather scant and fragmentary. During this experiment the numbers of all types of cells in the leukocytic system of porcine bone marrow were evaluated after 28 and 56 days of oral administration of simvastatin at a dose of 40 mg/day/animal. Simvastatin caused an increase in the number of all types of cells in the leukocytic system, and the most visible fluctuations concerned promyelocytes. Observations obtained during the present study indicated that the results of the action of simvastatin on porcine bone marrow differ from those observed in other mammal species, including human. This may be due to various metabolic pathways within the bone marrow in the particular species, but the exact mechanisms of these actions are unknown at the present time.
Показать больше [+] Меньше [-]Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing Полный текст
2018
Eftekhari Hadi | Jahandideh Alireza | Asghari Ahmad | Akbarzadeh Abolfazl | Hesaraki Saeed
Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing Полный текст
2018
Eftekhari Hadi | Jahandideh Alireza | Asghari Ahmad | Akbarzadeh Abolfazl | Hesaraki Saeed
In recent years, the use of bone scaffolds as bone tissue substitutes, especially the use of such as hydroxyapatite and tricalcium phosphate, has been very popular. Today, the use of modern engineering techniques and advances in nanotechnology have expanded the use of nanomaterials as bone scaffolds for bone tissue applications.
Показать больше [+] Меньше [-]Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing Полный текст
2018
Eftekhari, Hadi | Jahandideh, Alireza | Asghari, Ahmad | Akbarzadeh, Abolfazl | Hesaraki, Saeed
In recent years, the use of bone scaffolds as bone tissue substitutes, especially the use of such as hydroxyapatite and tricalcium phosphate, has been very popular. Today, the use of modern engineering techniques and advances in nanotechnology have expanded the use of nanomaterials as bone scaffolds for bone tissue applications. This study was performed on 60 adult male New Zealand rabbits divided into four experimental groups: the control group without any treatment, the second group receiving hydroxyapatite, the third group treated with β-tricalcium phosphate, and the fourth group receiving nanocomposite polycaprolactone (PCL) scaffold. In a surgical procedure, a defect 6 mm in diameter was made in a hind limb femur. Four indexes were used to assess histopathology, which were union index, spongiosa index, cortex index, and bone marrow. The results showed that nanocomposite PCL and control groups always had the respective highest and lowest values among all the groups at all time intervals. The histopathological assessment demonstrated that the quantity of newly formed lamellar bone in the nanocomposite PCL group was higher than in other groups. All these data suggest that PCL had positive effects on the bone healing process, which could have great potential in tissue engineering and clinical applications.
Показать больше [+] Меньше [-]Effect of TNF-α concentration on selected clinical parameters of swine after burns Полный текст
2018
Aleksiewicz Roman | Lutnicki Krzysztof | Likus Marta | Gątkiewicz Łukasz
Effect of TNF-α concentration on selected clinical parameters of swine after burns Полный текст
2018
Aleksiewicz Roman | Lutnicki Krzysztof | Likus Marta | Gątkiewicz Łukasz
The study aimed to observe TNF-α serum concentration as well as changes in respiration rate, body temperature, and pulse rate in burn victims during 84 h post burn.
Показать больше [+] Меньше [-]Effect of TNF-α concentration on selected clinical parameters of swine after burns Полный текст
2018
Aleksiewicz, Roman | Lutnicki, Krzysztof | Likus, Marta | Gątkiewicz, Łukasz
The study aimed to observe TNF-α serum concentration as well as changes in respiration rate, body temperature, and pulse rate in burn victims during 84 h post burn. A total of 30 healthy pigs were divided into two groups: A, the test group and N, the control group. The experimental group suffered burns to 30% of the body surface, and after infliction of the burns both groups were closely monitored. The biggest increase in TNF-α serum concentration in the test subjects occurred around the 6ᵗʰ h of the study, and the second biggest increase took place between 12ᵗʰ and 36ᵗʰ h. In the 36ᵗʰ h, TNF-α was 2.5 times more concentrated in serum in the test group than in the control group. In the test group, the biggest increase in respiration rate occurred up to the 6ᵗʰ h post burn, on average up to 29/min. In the 12ᵗʰ h post burn, the mean pulse rate in the test group was 133/min and dropped to the lowest value in the 72ⁿᵈ h of the experiment. A gradual increase in body temperature up to 41.72°C was observed up to the 30ᵗʰ h post burn and decreased to a significant value of 40.74°C by the 84ᵗʰ h of the study. In a period of a pronounced rise in TNF-α serum concentration, this parameter, pulse rate, and respiration rate are highly correlated and are also influenced by multiple inflammation forming factors.
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