Objective: The common coot (Fulica atra) is a medium-sized migratory bird wintering at different wetlands in Pakistan. It belongs to the order Gruiformes and family Rallidae. This study aimed to investigate the morphometry, hematology, and blood chemistry of common coots during the winter visit of 2022–2023. Materials and Methods: Thirty two adult common coots were captured with the help of hunters holding valid licenses from six wetlands in Punjab, Pakistan. Each individual was sexed and sub¬jected to different morphological measurements. Eighteen blood samples (3–5 ml) were collected from the basilic veins of adults for hematology and blood chemistry. Results: Results revealed that body weight ranged from 378 to 680 gm, with males significantly heavier than females. Length was not significantly different. Males showed dominance in most of the morphometric characteristics compared to females. Regarding hematological parameters and plasma chemistry, there was a sufficient difference between the genders in most of the studied parameters. Conclusion: The male coot was significantly larger compared to the female, except for total length. Hematology and plasma chemistry showed significant differences between genders. [J Adv Vet Anim Res 2025; 12(1.000): 312-320]

Objective: This study aimed to identify Casein Alpha S1 (CSN1S1) gene polymorphism as a biomarker selection for improving the milk quality of Indonesian local PE cross-dairy goats. Materials and Methods: The study utilized 65 does to identify single nucleotide polymorphisms (SNPs) on the target base fragment g.10094 gm.10200 of the CSN1S1 gene. Milk components (MCs) were analyzed using lactoscan and SNPs were identified using Sanger sequencing. Allele and genotype frequencies of the SNPs were determined by MEGA10 and PopGen32 programs. A general linear model was applied to examine the association between each SNP and the content (%) or daily yield (gm/day) of each of the MCs. Results: Eight SNPs were identified, with seven exhibiting dominant homozygous genotype references with genotype frequencies ranging from 0.77 to 0.95. SNP g.10098_C > G significantly affected (p < 0.05) the daily yield (gm/day) of MCs, but not for TS. The CC does, compared to the GC ones, produce higher yields (gm/day) of protein, fat, lactose, and SNF by 36.2%–39.8%. SNP g.10181_T > A had a significant effect (p < 0.05) on the content (%) of all MCs. The AT does, over the AA and TT ones, yield higher MCs by 3.63%–13.07% and 1.85%–10.68%, respectively. Conclusion: The findings suggest that SNP g.10098_C > G and g.10181_T > A of the CSN1S1 gene may serve as potential biomarkers for selective breeding to enhance milk quality in the local PE cross-dairy goats. [J Adv Vet Anim Res 2025; 12(1.000): 222-230]

Helminthiases, which are caused by parasitic helminths, have a big effect on global health, especially in places with few resources. They cause a lot of illness and put a lot of strain on society and the economy. Understanding the prevalence, transmission, and impact of helminthiases is crucial for effective control and prevention. Molecular population genetics has been pivotal in understanding helminth dynamics, including species identification, hybridization, and drug resistance. However, deeper insights require broader genetic datasets. Several genomes have been sequenced using genomic technologies, which has changed the way helminth researchers do their work and made it easier to compare genomes and find conserved genetic elements. Genetic factors of the host also affect susceptibility. Genome-wide association studies have found candidate genes that are connected to susceptibility or resistance. Helminth infections trigger Type 2 immune responses involving various immune cells, cytokines, and mediators. Recent discoveries show how non-immune cells like stromal, epithelial, and neural cells control these responses. Genetic differences between the host and the parasite affect how they interact. Helminths use immunomodulatory molecules to hide from immune surveillance. The concept of host disease tolerance, maintaining health despite infection, is gaining attention. The emergence of drug resistance poses a challenge, emphasizing the need to understand the genetic mechanisms underlying anthelmintic resistance. Genomic approaches offer promising avenues for interventions, including vaccine development and RNA interference. Challenges in helminth genetics research include genetic heterogeneity, limited sample sizes, and technical constraints. Using both functional genomics and multi-omics methods together can help us fully understand helminth genetics and plan effective treatments. Genomic studies have helped us learn more and find possible targets for interventions. To turn these findings into useful control measures, we need to do more research and work together. [J Adv Vet Anim Res 2025; 12(1.000): 123-131]

Objective: Cannabis sativa L. is aware of a rich source of bioactive substances with various struc¬tures that exhibit pharmacological activity in the central nervous system, cardiovascular, cerebro¬vascular, respiratory, reproductive, and gastrointestinal systems. Materials and Methods: In this study, cannabis sugar leaves were soaked in 99% ethanol, followed by evaporation. The antibacterial effect of the cannabis sugar leaf extract was then evaluated using the disc diffusion method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined using broth dilution. Results: The results of this study indicated that the cannabis sugar leaf extract inhibited Bacillus cereus, Vibrio cholerae, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis when compared to tetracycline, but it did not inhibit Pseudomonas aeruginosa. The MIC and MBC of the cannabis sugar leaves extract against B. cereus, V. cholerae, E. coli, S. aureus, and S. epidermidis were 0.977, 1.953, 31.25, 62.5, 125, 250, 250, 500, 250, and 500 mg/ml, respectively. The bioactive compounds in cannabis sugar leaf extract were identified using high-performance liquid chromatography. Conclusion: The results indicated that the major bioactive compounds were Δ-9- tetrahydrocan-nabinol (THC) and cannabidiol (CBD). While minor bioactive compounds included gallic acid and tannic acid. These results support the benefits of cannabis sugar leaf extract, which has been used for its pharmacological properties and may be useful as an alternative antimicrobial agent in medicine. [J Adv Vet Anim Res 2025; 12(1.000): 44-52]

Objective: A cross-sectional study was conducted to reveal the hematology, serum biochemistry, and reproductive behavior of free-roaming cats in the Sylhet Division of Bangladesh. Materials and Methods: Overall, 130 free-roaming cats were captured, of which 52 were Tom (male), 69 were Queen (female), and 9 were kittens. Regarding their ages, the cats were divided into three groups: [J Adv Vet Anim Res 2025; 12(1.000): 70-79]

Objective: This study aims to explore mutation based on single nucleotide polymorphism (SNP) in the Cholecystokinin B receptor (CCKBR) gene of Pelung chickens. Materials and Methods: We collected DNA samples from 48 Pelung roosters that had won the crowing competition. The CCKBR target encompasses exon 3, intron 3, exon 4, and a part of intron 4, a long 601 bp. This target was replicated using PCR with specific primers that were designed by Primer-BLAST from NCBI. We generated the nucleotide sequence from the PCR product's sequencing results. The SNP analysis was done by BioEdit and MEGA. Genotyping and haplotyp¬ing were done based on nonsynonymous single nucleotide polymorphisms (SNPs) on exons 3 and 4. We calculated allele and genotype frequency, heterozygosity, and Hardy-Weinberg Equilibrium (HWE) using POPGENE 32 programs. Results: This study found three nonsynonymous single nucleotide polymorphisms. The nsSNP in exon 3 alters the coding for the 210th amino acid from serine to asparagine (g.1290 G > A/S210N), while the SNPs in exon 4 alter the coding for the 232nd amino acid from valine to phenylalanine (g.1423G > T/V232F) and the 243rd amino acid that changes the amino acid valine to glycine (g.1457T > G/V243G). The frequency of the mutated alleles is lower than the unmutated alleles. However, the mutation at position g.1457T > G/V243G produces a higher frequency than the unmutated allele. The allele and genotype frequency were not in HWE. It was caused by intensive selection in Pelung chickens, especially for growing capacity. Conclusion: Nonsynonymous mutation on CCKBR may cause variations in the crowing and other traits such as the growth of Pelung chickens. Further studies are needed to explore the CCKBR gene, including the relationship of the gene with the vigor and/or stress level of Pelung chickens. [J Adv Vet Anim Res 2025; 12(1.000): 141-148]

Objectives: The rise of antibiotic-resistant strains of Staphylococcus aureus in dairy milk products is a global concern, compromising treatment efficacy and highlighting the need for innovative solutions. Therefore, a study was conducted to isolate S. aureus strains (N = 21) from raw milk samples of cows infected with subclinical bovine mastitis. Additionally, the resistance of these strains against 12 different antibiotics was examined. Materials and Methods: Sixty raw cow milk samples, 20 from each of three separate dairy farms in Lahore city, were collected and screened for the presence of S. aureus. It was discovered that 70% of these milk samples were contaminated with this bacterium, indicating a widespread presence across the farms. Different isolation tests were employed in this study, including gram staining, capsule staining, catalase, mannitol salt fermentation, DNase, coagulase, and oxidase. Results: The obtained results revealed that the isolated strains of S. aureus showed % of their resistance against different antibiotics in the order of amoxicillin (85%), penicillin (71%), gentamicin (CN) (42%), carbenicillin and trimethoprim-sulfamethoxazole (33%), streptomycin, ciprofloxacin, and oxytetracycline (28%), cefotaxime (10%), and chloramphenicol (4%) in decreasing order, respectively. However, these strains showed no resistance against Bacitracin and Ampicillin. Conclusion: The existence of resistant strains of S. aureus has been attributed to various factors, such as poor milk hygiene, delayed milk transportation, subclinical bovine mastitis among dairy cows, and antibiotic-resistant genes. Thus, our present study will provide useful information about the resistant strains of S. aureus, which may transfer through cows into milk and then produce serious food-borne diseases in human beings. This study will be helpful to improve and control the quality of dairy products in Pakistan. [J Adv Vet Anim Res 2025; 12(1.000): 252-259]

Objective: The goal of this study is to describe the genome of Streptococcus agalactiae that was found in clinical mastitis in cattle in Bangladesh. This work will show how strong the bacteria are and how important they are for public health. Materials and Methods: Whole genome sequencing (WGS) was performed using the Illumina MiSeq platform, followed by comprehensive analysis with various bioinformatic tools to identify key genomic features. Results: WGS revealed that the isolates are closely related, belonging to sequence type ST4, a rare type previously identified in both human and animal hosts. The isolates possess 44 viru-lence-related genes linked to adherence, capsule biogenesis, enzyme production, immunoreac-tive antigens, protease, and cytolysin production. They also carry two pilus islands (PIs), PI-1 and PI-2b, which are often associated with invasive diseases. PI-2b proteins are key targets for vaccine development against Group B Streptococcus (GBS). The isolates belong to serotype Ia and carry the gbs2018-2 variant, indicating their adaptability to a wide range of hosts, including humans and animals. These virulence factors are critical for understanding S. agalactiae's pathogenicity and developing vaccines against its infections. Additionally, the isolates harbor antimicrobial resistance genes conferring resistance to glycopeptides (vanT, vanY), macrolides (mreA), peptides (mprF), penicillins and β-lactams (pbp), and aminoglycosides. Source tracking via the BacWGSTdb website identified these isolates as closely related to human pathogens, indicating their zoonotic potential. Conclusion: These results suggest that S. agalactiae could be a zoonotic pathogen. This highlights the need for ongoing genomic surveillance to fully understand how it causes disease and come up with effective ways to control it. [J Adv Vet Anim Res 2025; 12(1.000): 80-89]

Objective: This study aimed to evaluate how well fresh broiler meat may be preserved in cold storage using chitosan–stearin as an edible coating. Materials and Methods: A completely randomized design with a 3 x 5 factorial arrangement and three replications was employed. Factor I represented the formula dosage (FD) (FD0 = 0% chitosan + 0% stearin; FD1 = 1.5% chitosan + 1% stearin; FD2 = 3% chitosan + 1% stearin), while Factor II represented storage time (ST) (ST0 = 0 days; ST1 = 3 days; ST2 = 6 days; ST3 = 9 days; ST4 = 12 days). Results: The results showed that the water content, cooking loss, protein content, and fat content of broiler meat were significantly affected (p < 0.01) by the FD and ST. Nonetheless, no significant difference (p > 0.05) was observed in the meat's ability to hold water. The broiler meat's pH was significantly affected (p < 0.01) by the FD, but it was not significantly affected (p > 0.05) by the ST. Furthermore, no treatment underwent testing, which revealed the presence of Escherichia coli and Salmonella sp. Conclusion: Chitosan–stearin edible coatings with different formula doses FD and ST consistently preserve the quality of fresh broiler meat during cold storage, with average values of water content ranging from 48.97% to 53.73%, water-holding capacity from 17.52% to 34.30%, cooking loss from 10.03% to 33.19%, pH levels from 4.93 to 5.53, protein content from 14.54% to 17.46%, fat content from 20.55% to 24.21%, and no detectable presence of E. coli and Salmonella sp. [J Adv Vet Anim Res 2025; 12(1.000): 99-105]

Objective: This study aimed to compare two approaches to the brachial plexus: the traditional blind method and an additional technique, both of which use anatomical landmarks to guide needle insertion. Materials and Methods: The traditional and additional approaches were performed on both thoracic limbs of 24 cadavers (24 for each approach). Methylene dye is used for injection and nerve staining. We counted the nerves that successfully stained (staining less than 1 cm). The Mann–Whitney U test was used to compare approaches. Results: The findings indicated that all cadavers were successfully used. The traditional approach and the additional approach revealed success rates of 45.83% and 54.17%, respectively. We found no significant differences between the two techniques (p > 0.05). Conclusion: The additional approach presents a viable alternative to the traditional method for performing the brachial plexus block in goats. Further research into the clinical differences between these techniques could lead to useful insights that help make them more accurate and useful. [J Adv Vet Anim Res 2025; 12(1.000): 64-69]