With the advancement of research and development of medical technology, there is an increase in the number of animals used in research. As millions of experimental animals are being used in different experiments worldwide, there is a lot of debate about the pain, distress, and death experienced by animals during scientific experiments. Experimental animals also require skilled manpower, time-consuming protocols and are costly. There are various alternatives to animal testing to overcome the drawbacks of animal experiments and avoid unethical procedures. Russell and Burch published principles of the Humane Experimental Technique which includes a strategy of 3 Rs (i.e. reduction, refinement, and replacement) in 1959. They introduced and defined the terms replacement, reduction, and refinement, which subsequently have become known as ‘alternatives’ or ‘alternative methods’ for minimizing the potential for animal pain and distress in biomedical research. Therefore, different methods and different alternative organisms are being used to implement the 3 Rs strategy. So, a brief account of these alternatives and the advantages associated is discussed in this review with examples. An integrated application of these approaches would give insight into the minimum use of animals in scientific experiments.

An experiment was conducted to study the effect of sex on growth pattern and carcass characters of commercial broilers. One hundred and twenty numbers of day-old chicks were wing banded, weighed and reared up to 40 days of age. The body weight was individually recorded from all the birds on 7, 14, 21, 28, 35 and 40 days of age to assess the influence of gender on body weight. At the end of the experiment 12 males and 12 females were subjected to slaughter studies to assess the carcass characters. The body weight between males and females were similar up to 14 days of age and thereafter, males recorded significantly (p<0.01) higher body weights on 21, 28, 35, 40 days of age, weighing 35.79, 107.69, 179.73, 211.88 g respectively higher than females. The ready-to-cook weight (%) and dressed carcass (%) were significantly (p<0.05) higher in males; whereas, New York carcass (%) was significantly (p<0.05) higher in females. Among the cut of parts, the drumstick percentage was significantly (p<0.05) higher in males, whereas, the wings percentage was significantly (p<0.05) higher in females. Among processing yields and losses, only shank & feet and abdominal fat (%) showed significant (p<0.05) difference between sexes; while, feather, blood, head, viscera, kidney and total offal yields had non-significant difference between sexes. Among giblets, only the heart yield was significantly (p<0.05) different between sexes with higher value in females. The results showed that the males attain marketable weight early, therefore can be marketed early. The males can be slaughtered earlier than females for producing specialized products from drumstick; whereas, females can be slaughtered early to harvest drumette.

The socioeconomic importance of nomadic donkey rearing, perception on health benefits of donkey milk and the marketing channels involved remain unexplored. This paper aims at providing details on nomadic donkey rearing, a traditional system prevailing in Tamil Nadu. Donkey played a significant role in the livelihood of its owners who had migrated throughout Tamil Nadu for selling donkey milk.  They traversed around 30 – 40 km per day to sell the donkey milk besides meeting the grazing needs of the donkeys. Majority of the nomadic donkey rearers were young to middle aged and were scheduled tribes. Two third of the respondents were involved in nomadic donkey rearing for nearly eight months in a year and for the remaining four months they earned their livelihood as  agricultural labourers. The study revealed that on an average, lactating jenny yield milk for six months. At the end of the lactation period, the donkey rearers sold the dry jenny to the traders and in turn purchased the lactating jenny from them. The nomadic donkey rearers believed that the donkey milk had medicinal benefits to cure ailments such as ulcer, indigestion, pneumonia,  jaundice, leukorrhea and hypernatremia. The respondents preferred institutional support in marketing donkey milk so that they could settle in one place and thrive better. 

To improve the production performance of white Guinea fowl, selective breeding was carried out continuously for six generation. A total 1000 Guinea fowl hatching eggs were procured from various government institutions and private entrepreneurs, and subsequently keets were hatched out and base population was created. The production traits were given due importance during selective breeding for six generations to improve both body weight and egg production. The traits such as hatch weight 4th, 8th, 12th,16th and 24 week body weight (g), FCR, livability, age at sexual maturity (days), Hen – Day Egg Production (HDEP) and Hen - Housed Egg Production (HHEP), annual egg number, egg weight (g), adult livability, total and fertile hatchability (%) were studied in all six generations. The day-old chick weight of all six generations ranged from 26.46 to 28.10 g. The 4th and 8th week mean body weight of the first generation white Guinea fowl was 180.85g and 481.69 g respectively, which increased to 220.95 g and 494.13 g, respectively in 6th generation. Similarly, the 12th and 16th week body weights in 1st generation increased from 767.12 g to 805.29 g and 951.04 g to 1079.15 g, respectively, at 6th generation. The linear regression analysis of 4th and 8th week body weight over generations revealed significant increment of 7.59 g and 7.72 g, respectively, per generation. Similarly, the 12th and 24th week body weight in each generation had a positive change with high degree of goodness of fit. The 12 and 16th week FCR showed a positive change of high magnitude over generations. The hen day and hen housed egg production (29-51 weeks) from 1st generation to 6th generation increased from 39.26 to 40.55 % and 37.53 to 38.83 %, respectively, during the course of selection. The total and fertile egg hatchability also showed marked changes due to selection with values varying from 49.52 to 53.14 % and 71.37 to 72.38 % across the generations. This study concluded that the multi stage short-term selection can improve both body weight and egg production in white Guinea fowl.  

A six-year-old Siberian husky bitch was presented to Small Animal Gynaecology and Obstetrics Unit, Madras Veterinary College Teaching Hospital, with a history of proestrus bleeding and prolonged breeding failure due to shyness. Based on progesterone value (8 ng/mL, artificial insemination was done. On day 45 post AI, ultrasonographic examination revealed the presence of a viable foetus and lateral abdominal radiography showed the presence of a single foetus. Based on the observations, the owner was made aware of chances of risk of dystocia due to single puppy syndrome and the case was kept under observation. However, the case was presented on day 65 of gestation with signs of inappetence and greenish black vaginal discharge for the past 6 hrs. Based on the Ultrasound examination, Radiography and progesterone concentration, the case was diagnosed as dystocia due to single puppy syndrome. Under local anaesthesia, episiotomy was performed and a viable foetus (650 gm) was delivered. The episiotomy wound was closed in simple interrupted suture pattern using polyamide (1-0). After one week of  postoperative care, the bitch had an uneventful recovery.

Fifteen days old Jersey crossbred calf was brought to the hospital with the history of sudden hair fall around the trunk and legs and the other calf was apparently normal in twin birth. Clinical examination revealed severe alopecia in both fore limbs, hind limbs, trunks and easy epilation of hairs, along with erythematous skin and pruritus and mild scaling around its thighs, flanks and trunk. Dermatological analysis of skin scrapings revealed negative for ectoparasites and trichogram showed no hair growth abnormalities. Haemato-biochemical parameters were in normal range except low plasma ascorbic acid (0.16 mg/dL). The case was diagnosed as vitamin C responsive dermatosis and treated with single dose of intravenous injection of 2.5 g of ascorbic acid diluted with normal saline. The owner was advised to follow tab. vitamin C total dose of 2 g/day for 10 days orally. After one week, further hair was loss ceased and complete regrowth of hair noticed after four weeks of treatment. 

Multicut fodder sorghum Co (FS) 29 was evaluated for its growth and yield performance with the application of different sources of irrigation and nutrients. The experiment was conducted in split plot design with three replications. Main plot consists of treatments with application of irrigation through ground water (I1), domestic sewage water (I2) and ground and domestic sewage water alternatively (I3). The sub plots consist of no fertilisers (control) (N1), application of farm yard manure alone (FYM alone) (N2), farmyard manure + NPK (FYM + NPK) (N3) and NPK alone (N4). The application of domestic sewage water along with FYM + NPK nutrients as per the treatment schedule resulted in higher plant height, leaf length, number of leaves, biomass production, dry matter and biomass yield of multicut fodder sorghum. However, pertaining to nutrient application, nitrogen uptake was higher with application of FYM +NPK but found on par with the application of NPK alone and application of FYM alone.

A study was conducted to evaluate external and internal egg quality characters of three chicken breeds/ ecotypes namely, indigenous Siruvidai, TANUVAS Aseel and White Leghorn.  A total of 180 eggs (120 eggs from Siruvidai chicken, 30 eggs from TANUVAS Aseel and 30 eggs from White Leghorn) were subjected to this study. The weight, length, width and surface area of White Leghorn eggs were significantly (P<0.05) higher than those of TANUVAS Aseel and indigenous Siruvidai chicken. Shape index was found to be significantly (P<0.05 higher in Siruvidai chicken eggs compared to that of TANUVAS Aseel and White Leghorn. Among the internal egg qualities, the height and width of albumen and albumen index were significantly (P<0.05) higher in White Leghorn chicken eggs compared to that of TANUVAS Aseel and Siruvidai chicken. Yolk height and yolk index of three breeds showed no significant difference among breeds. Yolk width was significantly (P<0.05) higher in TANUVAS Aseel compared to that of Siruvidai chicken. Yolk colour was significantly (P<0.05) higher in TANUVAS Aseel and Siruvidai chicken compared to that of White Leghorn. Shell thickness was significantly (P<0.05) higher in TANUVAS Aseel and White Leghorn as compared to Siruvidai chicken. Haugh unit was significantly (P<0.05) higher in White Leghorn compared to TANUVAS Aseel and Siruvidai chicken. The results revealed that the egg quality characters like egg weight, egg length, egg width, shape index, surface area, albumen height, albumen index, shell thickness and Haugh unit of indigenous Siruvidai chicken is different from other indigenous breed like Aseel and exotic breed like White Leghorn.

A study was conducted in four flood-prone villages at Krishi Vigyan Kendra, Assam Agricultural University, Dhubri to assess the production performance of Khaki Campbell ducks and the economics of rearing them under NICRA (National Innovations in Climate Resilient Agriculture) project. A total of 10 farmers were selected from Dhubri village on the basis of their experience in duck farming. Two hundred day-old Khaki Campbell ducklings, twenty numbers irrespective of sex per household, were distributed and reared up to 72 weeks. On attaining 30th week of age, the male ducks were sold out. The data including body weight, mortality, age at first egg and egg production were recorded. The average age at first egg was 163.0±3.6 days, and 30th  week mean body weight was 1.8±0.1 kg. The mean egg production and mortality rate were 180.8±4.1 and 13% respectively during the study period. It was found from the study that Khaki Campbell duck farming has contributed notably to the livelihood of the rural farmers with benefit-cost ratio of 1.87. 

A two year-old queen cat with unknown mating history revealed absence of clear indication of positive pregnancy but single fluid filled anechoic structure noticed on ultrasonography. Spaying was performed and clear watery sterile fluid in the uterus confirmed it as a case of hydrometra.