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Highly pathogenic avian influenza H5N8 in Poland in 2019–2020 Полный текст
2020
Śmietanka Krzysztof | Świętoń Edyta | Kozak Edyta | Wyrostek Krzysztof | Tarasiuk Karolina | Tomczyk Grzegorz | Konopka Bogdan | Welz Mirosław | Domańska-Blicharz Katarzyna | Niemczuk Krzysztof
Highly pathogenic avian influenza H5N8 in Poland in 2019–2020 Полный текст
2020
Śmietanka Krzysztof | Świętoń Edyta | Kozak Edyta | Wyrostek Krzysztof | Tarasiuk Karolina | Tomczyk Grzegorz | Konopka Bogdan | Welz Mirosław | Domańska-Blicharz Katarzyna | Niemczuk Krzysztof
Repeated incursions of highly pathogenic avian influenza virus (HPAIV) H5 subtype of Gs/GD lineage pose a serious threat to poultry worldwide. We provide a detailed analysis of the spatio-temporal spread and genetic characteristics of HPAIV Gs/GD H5N8 from the 2019/20 epidemic in Poland.
Показать больше [+] Меньше [-]Highly pathogenic avian influenza H5N8 in Poland in 2019–2020 Полный текст
2020
Śmietanka, Krzysztof | Świętoń, Edyta | Kozak, Edyta | Wyrostek, Krzysztof | Tarasiuk, Karolina | Tomczyk, Grzegorz | Konopka, Bogdan | Welz, Mirosław | Domańska-Blicharz, Katarzyna | Niemczuk, Krzysztof
Repeated incursions of highly pathogenic avian influenza virus (HPAIV) H5 subtype of Gs/GD lineage pose a serious threat to poultry worldwide. We provide a detailed analysis of the spatio-temporal spread and genetic characteristics of HPAIV Gs/GD H5N8 from the 2019/20 epidemic in Poland. Samples from poultry and free-living birds were tested by real-time RT-PCR. Whole genome sequences from 24 (out of 35) outbreaks were generated and genetic relatedness was established. The clinical status of birds and possible pathways of spread were analysed based on the information provided by veterinary inspections combined with the results of phylogenetic studies. Between 31 December 2019 and 31 March 2020, 35 outbreaks in commercial and backyard poultry holdings and 1 case in a wild bird were confirmed in nine provinces of Poland. Most of the outbreaks were detected in meat turkeys and ducks. All characterised viruses were closely related and belonged to a previously unrecognised genotype of HPAIV H5N8 clade 2.3.4.4b. Wild birds and human activity were identified as the major modes of HPAIV spread. The unprecedentedly late introduction of the HPAI virus urges for re-evaluation of current risk assessments. Continuous vigilance, strengthening biosecurity and intensifying surveillance in wild birds are needed to better manage the risk of HPAI occurrence in the future.
Показать больше [+] Меньше [-]Effects of vitamin C supplementation on the blood oxidative stress and antibody titre against Histophilus somni vaccination in calves Полный текст
2020
Otomaru Konosuke | Oishi Shoko | Fujimura Yu | Iwamoto Yuki | Nagai Katsuhisa | Ijiri Moe
Effects of vitamin C supplementation on the blood oxidative stress and antibody titre against Histophilus somni vaccination in calves Полный текст
2020
Otomaru Konosuke | Oishi Shoko | Fujimura Yu | Iwamoto Yuki | Nagai Katsuhisa | Ijiri Moe
The purpose of this study was to determine the effects of vitamin C supplementation on blood oxidative stress biomarkers and antibody response to vaccination in calves.
Показать больше [+] Меньше [-]Effects of vitamin C supplementation on the blood oxidative stress and antibody titre against Histophilus somni vaccination in calves Полный текст
2020
Otomaru, Konosuke | Oishi, Shoko | Fujimura, Yu | Iwamoto, Yuki | Nagai, Katsuhisa | Ijiri, Moe
The purpose of this study was to determine the effects of vitamin C supplementation on blood oxidative stress biomarkers and antibody response to vaccination in calves. Thirty-four clinically healthy 2 week old Japanese Black calves were randomly assigned to two groups. Seventeen calves formed the VC group which received 1,000 mg of vitamin C daily from 2 to 8 weeks of age, and the other 17 calves of the control group did not receive supplementation. All calves received an inactivated Histophilus somni vaccine at 4 and 8 weeks of age. Blood samples were taken at 2, 4, 8 and 12 weeks of age. The concentration of the serum reactive oxygen metabolites (d-ROMs), and the oxidative stress index (OSI), which is calculated from the d-ROMs and biological antioxidant potential, were significantly lower at 8 weeks of age in the VC group than in the control group (P < 0.05). The antibody titres to H. somni in the VC group were significantly higher than those in the control group at 12 weeks of age after the second vaccination (P < 0.05). Vitamin C supplementation to calves may reduce oxidative stress and enhance the antibody production after vaccination with H. somni.
Показать больше [+] Меньше [-]Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia Полный текст
2020
Picón Yenny | Almario Geiner | Rodríguez Victoria | Garcia Noel Verjan
Leishmaniasis is a zoonotic disease which is caused by protozoan parasites of the genus Leishmania. Canids are the most important reservoir of the parasites; however, limited data are available on the species of Leishmania prevalent in these animals and their impact on human health. The objective of this study was to estimate the seroprevalence of leishmaniasis in dogs from an inter-Andean region of Colombia during July 2016–July 2017, and to describe the clinical and histopathological features of the disease.
Показать больше [+] Меньше [-]Clinical applications of imaging modalities of the carpal joint in dogs with particular reference to the carpal canal Полный текст
2020
Tobolska Angelika | Adamiak Zbigniew | Głodek Joanna
The structure of the canine carpal joint is complex. This small joint consists of articulations that include the antebrachiocarpal, middle, carpometacarpal, and intercarpal joint surfaces. A large number of ligaments and tendons support and stabilise the carpus in dogs. Many injuries of this joint in dogs are not correctly recognised, diagnosed, or treated due to the limited use of diagnostic imaging methods. Radiography, the most common of them, has extensive application in diagnosing the causes of lameness in small animals. Other techniques, such as ultrasonography, computed tomography, and magnetic resonance imaging visualise other joint structures and surrounding soft tissues. However, these imaging modalities are rarely used to diagnose diseases and injuries of the canine carpus at present. The main reason for this is the small amount of research carried out and the lack of a properly described methodology for the use of imaging techniques. The wide use of all diagnostic imaging tools in the diagnosis of diseases and injuries of the wrist joint in humans shows that conducting studies on dogs could expand current knowledge. The use of these techniques in veterinary medicine could facilitate diagnosis and subsequent therapy of carpal disorders in dogs. MRI is the most frequently used imaging method in human medicine for visualisation of abnormalities of joints. This method could become a valuable part of the detection of inflammatory, traumatic, and degenerative diseases of the carpal joint in dogs.
Показать больше [+] Меньше [-]Escherichia coli -induced inflammation changes the expression of acetylcholine receptors (M2R, M3R, and α-7 nAChR) in the pig uterus Полный текст
2020
Jana Barbara | Całka Jarosław | Palus Katarzyna | Sikora Małgorzata
The influence of inflammation on the patterns of muscarinic 2 and 3 receptor subtypes (M2R and M3R), and α-7 nicotinic acetylcholine receptor (α-7 nAChR) expression in the porcine uterus was investigated.
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2020
Katkiewicz Maria
This paper presents the results of the microscopic examination of dairy cow ovaries.
Показать больше [+] Меньше [-]Attempts at the development of a recombinant African swine fever virus strain with abrogated EP402R, 9GL, and A238L gene structure using the CRISPR/Cas9 system Полный текст
2020
Woźniakowski Grzegorz | Mazur-Panasiuk Natalia | Walczak Marek | Juszkiewicz Małgorzata | Frant Maciej | Niemczuk Krzysztof
African swine fever (ASF) is a pressing economic problem in a number of Eastern European countries. It has also depleted the Chinese sow population by 50%. Managing the disease relies on culling infected pigs or hunting wild boars as sanitary zone creation. The constraints on the development of an efficient vaccine are mainly the virus’ mechanisms of host immune response evasion. The study aimed to adapt a field ASFV strain to established cell lines and to construct recombinant African swine fever virus (ASFV) strain.
Показать больше [+] Меньше [-]African swine fever virus – the possible role of flies and other insects in virus transmission Полный текст
2020
Fila Mateusz | Woźniakowski Grzegorz
African swine fever (ASF) is an acute viral haemorrhagic disease of pigs and wild boars. It presents a serious threat to pig production worldwide, and since 2007, ASF outbreaks have been recorded in the Caucasus, Eastern Europe, and the Baltic States. In 2014, the disease was detected in Poland. ASF is on the list of notifiable diseases of the World Organisation for Animal Health (OIE). Due to the lack of an available vaccine and treatment, the countermeasures against the disease consist in early detection of the virus in the pig population and control of its spread through the elimination of herds affected by disease outbreaks. Knowledge of the potential vectors of the virus and its persistence in the environment is crucial to prevent further disease spread and to understand the new epidemiology for how it compares to the previous experience in Spain gathered in the 1970s and 1980s.
Показать больше [+] Меньше [-]Chlamydiae – what’s new? Полный текст
2020
Zaręba-Marchewka, Kinga | Szymańska-Czerwińska, Monika | Niemczuk, Krzysztof
This paper provides an overview of the current knowledge of chlamydiae. These intracellular microorganisms belonging to the Chlamydiaceae family are widely distributed throughout the world. Constant development of culture-independent approaches for characterisation of microbial genomes enables new discoveries in the field of Chlamydia. The number of new taxa is continuously increasing as well as the range of hosts. New species and genotypes are constantly being discovered, particularly new avian and reptilian agents, which are discussed in this article. Interestingly, wild animals are the main hosts for new Chlamydia species including different species of bird, turtle and snake. The availability of next-generation sequencing opens up a new prospect for research and leads to deeper knowledge of these interesting microorganisms about which much is still to discover.
Показать больше [+] Меньше [-]Effect of bathing in a 0.1% aqueous solution of ethacridine lactate on selected physiological parameters of Cornu aspersum Müller edible snails Полный текст
2020
Ziętek, Jerzy | Dzięgiel, Beata | Wójcik, Alicja | Wilczyńska, Anna | Adaszek, Łukasz | Winiarczyk, Stanisław
The prohibition of antibiotic use in edible snails obligates breeders to treat bacterial infections by different means, of which a common one is a bath in Gram-positive– and partially Gram-negative–bactericidal ethacridine lactate solution. The aim of the study was to determine the effect of bathing Cornu aspersum Müller snails in a 0.1% aqueous solution of ethacridine lactate on selected physiological parameters of haemolymph. The study included 80 snails, divided into two equal groups (study and control). The study group was subjected to bathing in ethacridine lactate and the control group to bathing in tap water. Both groups were treated daily for seven days. The number of haemocytes in the haemolymph, the activity of alanine (ALT) and aspartate (AST) aminotransferases, and the concentration of urea were determined. In the study group, after exposure to ethacridine lactate solution an increase in ALT activity, changes in the De Ritis ratio, an increase in the amount of haemocytes, and a decrease in body weight were found. No such changes were detected in the control group snails or in animals after the first bath. Multiple applications of a 0.1% ethacridine lactate bath may adversely affect Cornu aspersum Müller snails.
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