Diabetes mellitus (DM) is a metabolic disorder and endocrinopathy characterized by hyperglycemia or a persistent increase in blood glucose levels. This case describes a male domestic cat exhibiting symptoms of vomiting, loss of appetite, and yellowish discoloration of the ears and oral mucosa, which was diagnosed with DM and hepatic-biliary disorders based on serum biochemistry and ultrasonographic examinations. The treatment included insulin injections, antibiotics, antiemetics, a liver supplement, and a specialized diabetic diet. After 14 days of insulin injection and a diabetic diet, the cats blood glucose levels returned to normal. A diabetic diet will be provided for the cat throughout its lifetime.

Background and Aim: Guinea fowls are known for their low reproductive efficiency under natural mating conditions, which limits their potential for intensive production. This study aimed to evaluate the effect of artificial insemination on the reproductive performance of Guinea fowls as a strategy to enhance fertility and hatchability outcomes.Materials and Methods: A total of one hundred and fourteen (114) adult Guinea fowls were randomly assigned to three treatment groups. The first group consisted of Guinea fowls (GFs) naturally mated in a ratio of one (1) male to four females (4) in six replicates. In contrast, the second and third groups consisted of twenty-four (24) Guinea hens, artificially inseminated with 0.03 ml fresh undiluted Guinea cock semen and 0. l ml Guinea cock semen diluted (1:3) with Ringers lactate solution. Semen was collected once weekly from GCs trained for semen collection and used to artificially inseminate Guinea hens by the intra-vaginal method using a graduated 1ml syringe. Eggs were collected from the treatment groups from day two (2) to day seven (7) post-AI, processed, and then incubated. At the end of twenty-eight (28) days of incubation, the number of keets hatched per treatment group was counted. The unhatched eggs were broken open to determine infertile eggs and embryonic mortality.Results: The results from the study show that fertility (%), fertile hatchability (%), and total hatchability (%) were significantly higher in artificially inseminated Guinea fowls than in those naturally mated, while embryonic mortality was similar in all treatment groups.Conclusion: Artificial insemination improved Guinea fowl reproductive performance. It is recommended that artificial insemination be implemented as part of intensive Guinea production

Background and Aim: Malacca leaves are one of the medical plants that can be used in traditional medicine. Malacca leaves contain several active compounds that can be effective in inhibiting fungal growth. This study aims to investigate the inhibition of n-hexane Malacca leaves extract on Microsporum canis growth in vitro.Materials and Methods: The method used in this study is Kirby-Bauer disc diffusion with 5 treatments, namely P1 (25% Malacca leaves n-hexane extract), P2 (50% Malacca leaves n-hexane extract), P3 (75% Malacca leaves n-hexane extract), P4 (control (+): ketoconazole) and P5 (control (-): carboxymethylcellulose (CMC) 1%) with 3 replications. The clear zone formed around the disc was measured using a caliper.Results : The results showed that there was no clear zone formed around the disks of the n-hexane extract of Malacca leaves. It can be concluded that the n-hexane extract of Malacca leaves could not inhibit the growth of Microsporum canis.Conclusion: The findings of this study indicate that the n-hexane extract of Malacca leaves, at concentrations of 25%, 50%, and 75%, does not exhibit antifungal activity against Microsporum canis in vitro, as evidenced by the absence of inhibition zones. Therefore, n-hexane may not be an effective solvent for extracting antifungal compounds from Malacca leaves against this fungal species.

Background and Aim: The study was conducted in Pabentengang Village, Bajeng District, Gowa Regency, South Sulawesi 2020.Materials and Methods: The study used a survey method of 25 farmers, using questionnaires and interviews. The research location is agricultural land and the community's main business is agriculture and beef cattle. Primary data and secondary data were analyzed descriptively, quantitatively, and economically. The purpose of this paper is to determine the increase in the economic value for farmers through improving beef cattle business.Results: The results showed that the profit of farmers by "paro" or profit sharing was IDR. 4,262,500/farmer/year, and the R/C value was 1.3. The profit of farmers from fattening is IDR. 9,847,000/farmer/period with an R/C value of 1.2. The profit of farmers by producing calves through AI with Limousin semen is IDR. 7,250,000/farmer/year, the R/C value is 1.9. Government support and policies are needed regarding economic feasibility for the welfare of farmers, so that the sustainability of the beef cattle business can be maintained. The strategies that need to be implemented are not only related to the technical aspects of animal husbandry, but also the institutional and communication aspects that are needed in a mutually sustainable manner.Conclusion: Beef cattle business by paro or with profit sharing, producing calves through AI and fattening, is economically feasible to be re-cultivated.

The main purpose of this study was to survey on achieving recombinant DNA vaccine (live delivery) against Streptococcusis for rainbow trout (Oncorhynchus mykiss) Immunization. Initially, a total of 515 samples were collected from the head kidney of diseased fish (weighing 50_200g) in 72 farms in 8 provinces. Approximately, 40% (206 samples) of specimens were infected with Streptococcus species. Then isolated 172 DNA samples were and consequently, five pathogenic species have been identified, including Streptococcus iniae, Streptococcus faecium, Streptococcus agalactiae, Streptococcus dysgalatiae, and Streptococcus uberis. The enzyme phosphoglucomutase (PGM) has recently been discovered to play an important role in polysaccharide capsule production and virulence in S. iniae. Therefore, S. iniae was initially isolated S. iniae and cloned the phosphoglucomutase gene. Then, the PGM gene was amplified successfully and cloned in the pTZ57R cloning vector. The recombinant plasmid was subcloned into the pETD uet-l expression vector by restriction enzymes and confirmed by PCR. Meanwhile, for amplifying simA and cpsD genes were used universal primers pNZ8148 and special for simA and cpsD genes. The recombinant bacteria Lactococcus lactis (NZ9000) was used to transform the plasmid into L. lactis. Vaccination was performed by bath and injection (peritoneal) methods. The efficiency of g2 was better than g1in these two methods and in all of the groups. The detection of anti S. iniae antibody and determination of IgM level was carried out by using ELISA. The results revealed that there was a significant (p<0.05) difference between the level of IgM in both two methods and experiment groups compared to the control group. The results of the challenge of vaccinating fish with S. iniae showed that fish RPS in all groups were more than 50 percent while in the control group was 21.43 percent. The highest fish RPS belonged to group 5(61.25 percent) and statistical analyses revealed a significant (p<0.05) difference between fish vaccinated RPS, compared to the control group.

Temperature significantly influences the distribution and survival of bivalve populations, with elevated temperatures resulting in increased mortality rates. Research indicates that temperature fluctuations can markedly affect bivalve immune responses, including gene expression, hemocyte concentrations, and overall immunocompetence. Higher temperatures impair immune functions, leading to decreased phagocytic activity, compromised immune parameters, and disrupted metabolic processes, which collectively contribute to increased mortality. Understanding the relationship between temperature stress and bivalve survival is crucial for effective population management and for developing strategies to mitigate the negative impacts on bivalve populations in changing ecosystems.

In recent years mammary gland neoplasm has been recognized in pet and laboratory rabbits. The present study describes the concurrence of two malignant mammary gland neoplasms in a six-year-old intact female domestic rabbit. On clinical examination, the masses were observed in the left inguinal and right thoracic mammary glands. Based on owner information, the inguinal mammary gland mass within the previous 3-month period, and the thoracic mammary gland mass within the previous 6-month period, had become evident. Finally, complete surgical removal of the masses was selected. Tissue samples of the masses were fixed in 10% neutral buffered formalin and stained with H&E. In addition, immunohistochemical studies on masses sections were performed using primary antibodies against cytokeratins AE1/AE3. Microscopically, the left mass was diagnosed as solid carcinoma, and the right mass was diagnosed as intraductal papillary carcinoma. Immunohistochemistry showed diffuse positive cytoplasmic staining of the neoplastic cells in both of masses with primary antibodies against cytokeratins AE1/AE3. The best treatment option for mammary masses in pet rabbits is surgical excision, that is performed under general anesthesia. In this case, no new growth of the masses was observed 4 months following surgical procedures.

Canine transmissible venereal tumor also known as transmissible venereal tumor, contagious venereal tumor, transmissible lymphosarcoma, transmissible venereal sarcoma and infectious granuloma. This tumor commonly affects the external genitalia in dogs of both sexes but More incidences in females are occurring as compared to males. The clinical signs of the genital system include serosanguineous vaginal discharge, cutaneous discharge, protrusion of neoplastic lesions, intermittent or continuous skin lesions, swelling of the genital area, excessive licking of the genital region and pain. The tumor initially is small and its color varies from pink to red, subsequently transforming into a large, ulcerated and infected mass. Over time, the volume of the tumor increases and the lesions develop into fragile, hyperemic, bleeding, multilobular and cauliflower resembling masses. A Sarabi bitch, 18 months old, was referred to the hospital of faculty of Shahid Chamran University in Ahvaz, suffering from serosanguineous vaginal discharge for the past three months. During examinations, the size of the vagina was normal and the discharge was observed to be red in color. CBC and biochemistry tests indicated a decrease in red blood cells and hematocrit level, along with an increase in fibrinogen. In the vaginal cytology sample, red blood cells, large and small intermediate cells and TVT round cells were observed. After multiple assessments, a diagnosis of transmissible venereal tumor (TVT) was confirmed. However, due to the owner’s lack of follow up, treatment was not completed.

Although sabiyal wetland is supposed to sieve pollutants carried by the lake, growing human activity has caused it to deteriorate and shrink in size, reducing its ability to do so and allowing waste to flow into the lake. This makes evaluating the effects of wastewater in that lake urgently necessary. Since little is known about the use of serum and haematological assays to assess fish health in relation to heavy metals, this study is essential. This study aims to evaluate African catfish (Clarias gariepinus) blood profiles and heavy metal concentrations in Sabiyal Lake, Aliero, Nigeria. The study was conducted in Kebbi State's Aliero Local Government Area, specifically at Sabiyal Lake. For this investigation, five percent (5%) of the C. gariepinus that were caught were used. Samples of blood, gills, and bone were obtained using conventional techniques. Heavy metal analysis was done on all samples, while haematological, plasma, and serum analyses were done on the blood samples. The findings indicated that there was a huge variation in the haematological variables during the course of the research periods (p<0.05). Decreased plasma biochemistry (p<0.05) and different enzymes (p<0.05). The blood, gills, and bone of fish collected from Sabiyal Lake showed varying levels of heavy metal contamination. Fish collected in October had the highest concentration of lead, copper, zinc, cadmium, and iron, while fish recorded in December had the lowest concentration.  The investigation found that the high concentration of heavy metals had an impact on the fish from Sabiyal Lake's haematology, biochemistry, and blood enzymes. Therefore, more study should be done to monitor and control the fish and water in Sabiyal Lake.

Principal Component Analysis (PCA) is a powerful statistical tool used to reduce the complexity of large datasets while preserving significant variations. In this study, PCA was applied to explore the morphological traits of Japanese quails (Coturnix japonica), specifically focusing on skull measurements to identify key components affecting skull weight. A total of 112 quails (64 males and 48 females) were measured for various skull features, which were then analyzed through PCA. The analysis extracted three principal components for both sexes, explaining 52.76% of the variance in males and 56.52% in females. Key features such as Cerebellar Prominentia and Paraoccipital Process were identified as significant contributors to skull morphology. PCA was correspondingly applied to the measurements of male and female Japanese Quails' skulls, in order to identify those components which may explain most of the variation in skull weight. In this respect, simplification of data by PCA may indicate which morphological features supply most to the observed variation in skull weight and, therefore, provide interesting insights into the avian skull morphology. The goal of this research will be helpful in the laying of clear understanding regarding the anatomical features highly influential for skull structure, and of high importance to evolutionary biology, studies of veterinary importance, and poultry breeding programs. These findings highlight the applicability of PCA in anatomical studies and provide a deeper understanding of avian skull morphology.