Helminthiases, which are caused by parasitic helminths, have a big effect on global health, especially in places with few resources. They cause a lot of illness and put a lot of strain on society and the economy. Understanding the prevalence, transmission, and impact of helminthiases is crucial for effective control and prevention. Molecular population genetics has been pivotal in understanding helminth dynamics, including species identification, hybridization, and drug resistance. However, deeper insights require broader genetic datasets. Several genomes have been sequenced using genomic technologies, which has changed the way helminth researchers do their work and made it easier to compare genomes and find conserved genetic elements. Genetic factors of the host also affect susceptibility. Genome-wide association studies have found candidate genes that are connected to susceptibility or resistance. Helminth infections trigger Type 2 immune responses involving various immune cells, cytokines, and mediators. Recent discoveries show how non-immune cells like stromal, epithelial, and neural cells control these responses. Genetic differences between the host and the parasite affect how they interact. Helminths use immunomodulatory molecules to hide from immune surveillance. The concept of host disease tolerance, maintaining health despite infection, is gaining attention. The emergence of drug resistance poses a challenge, emphasizing the need to understand the genetic mechanisms underlying anthelmintic resistance. Genomic approaches offer promising avenues for interventions, including vaccine development and RNA interference. Challenges in helminth genetics research include genetic heterogeneity, limited sample sizes, and technical constraints. Using both functional genomics and multi-omics methods together can help us fully understand helminth genetics and plan effective treatments. Genomic studies have helped us learn more and find possible targets for interventions. To turn these findings into useful control measures, we need to do more research and work together. [J Adv Vet Anim Res 2025; 12(1.000): 123-131]

Objective: The goal of this study is to describe the genome of Streptococcus agalactiae that was found in clinical mastitis in cattle in Bangladesh. This work will show how strong the bacteria are and how important they are for public health. Materials and Methods: Whole genome sequencing (WGS) was performed using the Illumina MiSeq platform, followed by comprehensive analysis with various bioinformatic tools to identify key genomic features. Results: WGS revealed that the isolates are closely related, belonging to sequence type ST4, a rare type previously identified in both human and animal hosts. The isolates possess 44 viru-lence-related genes linked to adherence, capsule biogenesis, enzyme production, immunoreac-tive antigens, protease, and cytolysin production. They also carry two pilus islands (PIs), PI-1 and PI-2b, which are often associated with invasive diseases. PI-2b proteins are key targets for vaccine development against Group B Streptococcus (GBS). The isolates belong to serotype Ia and carry the gbs2018-2 variant, indicating their adaptability to a wide range of hosts, including humans and animals. These virulence factors are critical for understanding S. agalactiae's pathogenicity and developing vaccines against its infections. Additionally, the isolates harbor antimicrobial resistance genes conferring resistance to glycopeptides (vanT, vanY), macrolides (mreA), peptides (mprF), penicillins and β-lactams (pbp), and aminoglycosides. Source tracking via the BacWGSTdb website identified these isolates as closely related to human pathogens, indicating their zoonotic potential. Conclusion: These results suggest that S. agalactiae could be a zoonotic pathogen. This highlights the need for ongoing genomic surveillance to fully understand how it causes disease and come up with effective ways to control it. [J Adv Vet Anim Res 2025; 12(1.000): 80-89]

Objective: This study aimed to identify the impact of variations in solvent polarity on the solu-bility of secondary metabolite compounds, which were correlated as antioxidant and antibacte-rial agents for Escherichia coli (ATCC 11725), Salmonella sp. (ATCC 22504), Staphylococcus aureus (ATCC 11526), and Bacillus subtilis (ATCC 11626). Materials and Methods: A total of 500 gm of Temulawak flour (Curcuma xanthorrhiza) was macerated gradually using the step gradient polarity (SGP) technique, where the solvents (methanol, ethyl acetate, and n-hexane) used had different levels of polarity. The extracting process of secondary metabolite components of Temulawak flour began with the use of non-polar solvents, semipolar solvents, and polar solvents, respectively. Each soaking process was completed for 5 × 24 h. Results: From the Temulawak extract with different levels of solvent polarity, 76.048 gm were obtained for the methanol fraction, 106.242 gm for the ethyl acetate fraction, and 154.575 gm for the n-hexane fraction. The results of the antioxidant activity test showed that the ethyl acetate fraction had the highest inhibitory value for antioxidant activity of 50% (IC50) 22.59 mg/l. The results of this research's β-carotene content were 0.0865 gm. Ethyl acetate fraction was known to have good activity from the antibacterial activity test. The inhibition zone of E. coli and B. subtilis bacteria was 11.5 ± 0.71 with a minimum concentration of 3.13 mg/ml. The inhibition zone of S. aureus was 10.5 ± 0.17 with a minimum inhibitory concentration (MIC) of 6.25 mg/ml, while the inhibition zone for Salmonella sp. was 8.125 ± 0.35 with a MIC of 37.50 mg/ml. Conclusion: All fractions have moderate antibacterial activity, yet the ability of the ethyl acetate fraction of Temulawak extract was higher than the methanol and n-hexane fractions. [J Adv Vet Anim Res 2025; 12(1.000): 192-204]

Objective: This study was to evaluate the relationship between serum anti-Mullerian hormone (AMH) concentration and antral follicle count (AFC) during the estrous cycle of Colombian Creole cows of the Chino Santandereano breed. Materials and Methods: Ten non-lactating, non-pregnant, multiparous cows of the Chino Santandereano breed (Bos taurus taurus adapted), aged between 3 and 7 years, with a body condition of 3.0 ± 0.4 and with normal reproductive tracts at the structural and functional level, were selected and used. For the synchronization of estrus and ovulation, an intravaginal progesterone release device plus 2 mg of estradiol benzoate was applied for 8 days. On day 8, 150 μg of cloprostenol sodium + 300 IU of equine chorionic gonadotropin + 1 mg of estradiol cypionate was administered. This protocol was performed to determine the antral follicular count and to quantify serum AMH levels every 5 days during the estrous cycle. Results: The mean serum AMH concentration and AFC were 725 ± 2.7 pg/ml and 43.4 ± 3.5, respectively. A high correlation was observed between AMH and AFC of r = 0.041; p < 0.0001. Conclusion: The results showed that in Creole cows of the Chino Santandereano breed, there is a high correlation between circulating levels of AMH and ovarian reserve. This mechanism can be used as an endocrine biomarker of the follicular population. [J Adv Vet Anim Res 2025; 12(2.000): 680-686]

Objective: This study aimed to assess the knowledge, attitudes, and practices (KAP) of key stakeholders regarding African swine fever (ASF) and its reporting in Abuyog, Leyte, Philippines. It also aimed to identify sociodemographic factors associated with KAP levels. Materials and Methods: A cross-sectional survey was performed on 392 respondents, including pig farmers (n = 333), butchers (n = 38), live pig/meat sellers (n = 11), and Local Government Unit personnel (n = 10) between November 2023 and February 2024. KAP scores were calculated and categorized into "poor" and "good" using a median cutoff. Logistic regression analyses were conducted to investigate the association between sociodemographic variables and KAP levels. Results: Most participants showed poor knowledge of ASF causative agents, transmission, and clinical signs (83.93%) and disease recognition (60.20%), but many have good knowledge of ASF reporting protocols (70.92%). Attending ASF seminars/training was associated with improved basic ASF knowledge, disease recognition, and case reporting. Basic knowledge of ASF could enhance disease recognition. Disease recognition could then enhance ASF case reporting. Younger stakeholders showed better knowledge of basic ASF concepts. Pig farmers exhibited poor knowledge of disease recognition. Most participants showed good attitudes toward ASF reporting (97.7%), which was associated with overall knowledge of ASF. Most participants showed good practices in the early steps of case reporting (85.20%), relatively balanced reporting protocol (49.23%), and relatively poor knowledge-seeking behavior (45.41%). Pig farmers were less likely to report than other stakeholders. Good overall knowledge translates into good practices. Overall practices are influenced by the primary source of income. Conclusion: The findings reveal a notable gap in knowledge concerning ASF among participants, highlighting an essential need for enhanced educational initiatives. Strengthening basic ASF knowledge is vital, as it positively impacts disease recognition and, in turn, case reporting. Although there is a generally positive attitude toward ASF reporting, the lack of knowledge-seeking behavior and the variability in reporting practices based on income sources suggest a need for tailored educational programs. [J Adv Vet Anim Res 2025; 12(2.000): 629-646]

Antimicrobial resistance (AMR) is a global public health issue, causing an estimated 1.27 million deaths in 2019. This systematic review and meta-analysis aim to assess the burden of AMR in food animals in Pakistan, identify resistant microbes, and highlight emerging trends in multidrug resistance (MDR). The major databases were searched for articles published between 2016 and 2020 on the prevalence of AMR in food animals in Pakistan. A random-effects model was employed to pool the prevalence of antibiotic-resistant Enterobacteriaceae and non-Enterobacteriaceae pathogens. Among 1,145 studies, 35 met the inclusion criteria as evidence of AMR in food animals. Escherichia coli showed the highest resistance to ampicillin (59.5%), ciprofloxacin (49%), oxytetracycline (39%), and chloramphenicol (35%); Salmonella to ampicillin (78.4%), amoxicillin (53.9%), chloramphenicol (40%), tetracycline (39.3%), and ciprofloxacin (39%); Staphylococci to cefoxitin (53.8%) and penicillin (34.8%); and Campylobacter and Klebsiella to ciprofloxacin (50.4% and 83.3%, respectively). MDR was observed in E. coli (12/12 studies), Salmonella (7/10), Staphylococci (3/8), Campylobacter (3/3), and Klebsiella (1/3), with extensive drug resistance in E. coli (3/12), Salmonella (4/10), Campylobacter (1/3), and Klebsiella (2/2). Enterobacteriaceae showed significant resistance to tetracyclines (pooled prevalence/PPr = 0.75) and aminopenicillins (PPr = 0.74), whereas non-Enterobacteriaceae showed resistance to cephalosporins (PPr = 0.67) and aminopenicillins (PPr = 0.59), both with substantial heterogeneity. This review shows the existence of bacteria resistant to commonly used antimicrobials in food animals, potentially a threat to both human and animal health. The findings suggest the continuous monitoring of AMR and antimicrobial use (AMU) and the regulation of AMU in the food and agriculture sectors. [J Adv Vet Anim Res 2025; 12(2.000): 668-679]

Objective: The study aimed to assess the impact of dietary nucleotide (NT) supplementation on the growth performance and immune response of juvenile Macrobrachium rosenbergii. Materials and Methods: A commercial diet was supplemented with 0.0% (control), 0.10% (T1), 0.15% (T2), and 0.20% (T3) NT for the study. A total of 132 juvenile prawns (0.78 ± 0.03 gm) were randomly distributed into four groups, each having three replicates. Following the 75-day feeding trial, the experimental prawns were exposed to Aeromonas veronii at 8.35 × 105 colony forming unit (cfu)/ml for 7 days through a bath treatment. Results: The growth parameters and survival rate were significantly higher (p < 0.05) in NT-supplemented prawns. Immune-related parameters, e.g., total hemocyte count (THC), hemolymph protein, albumin, globulin, superoxide dismutase, and catalase activity, were also significantly higher (p < 0.05) in NT-supplemented prawns. The challenge of prawn with A. veronii resulted in a significant reduction (p < 0.05) in THC and other biochemical parameters of hemolymph and caused mortality in all the experimental groups. However, significantly higher survival (p < 0.05) against the A. veronii challenge was found in NT-supplemented prawns. Conclusion: It can be concluded that dietary NTs should be supplemented at 0.15%–0.2% to enhance the growth, immunity, and resistance of juvenile M. rosenbergii against A. veronii. [J Adv Vet Anim Res 2025; 12(2.000): 687-697]

Objective: This review examines Japan's pig farming landscape, highlighting key barriers while exploring projects that foster large-scale sustainable development efforts by emphasizing precision technologies integration and policy implications. Materials and Methods: A literature review was conducted using keyword searches across Google Scholar, covering studies published between 2018 and 2024. The review encompassed studies on Japan's pig farming, addressing prospects, production metrics, challenges, consumption patterns, market trends, precision technologies, and insights from peer-reviewed journals, credible websites, government reports, and conference proceedings. Results: Japan, one of Asia's largest pork consumers, relies on imports, with domestic production covering only 47.08% of consumption, highlighting a need for greater efficiency. Although small-scale farms continue to dominate the pig industry, the sector is navigating a pivotal shift toward modernization and the expansion of large-scale operations. Farmers face mounting pressures from feed costs, labor shortages, diseases, and strict environmental regulations. Precision pig farming technologies address these by optimizing resource use, enabling early disease detection to reduce costs, improving herd health to promote better welfare, and managing manure to reduce emissions. Conclusion: Integrating large-scale operations with precision pig farming technologies can redefine Japanese pig farming, promoting animal welfare and environmental sustainability. The government must secure financial backing (partial or full subsidies) to support large-scale operations, tax reductions on imported tools, and grants to foster domestic tools and renewable energy innovations to achieve this. Future life-cycle assessment research will be essential for evaluating the long-term environmental impacts, ensuring viability, and promoting sustainability in Japan's pork production sector. [J Adv Vet Anim Res 2025; 12(2.000): 454-476]

Objective: The study was primarily conducted to assess antimicrobial usage, associated factors, and animal health management practices in small-scale household dairy farms in a district of southern India. Materials and Methods: A total of 330 dairy farmers participated in the study, and single-stage cluster sampling was performed, followed by probability proportional to size sampling. A semi-structured, validated questionnaire and a checklist were used to collect the data. Results: Only a few dairy farmers knew about antimicrobials (33%) and antimicrobial resistance (AMR) (10.9%). All 330 dairy farmers were using antimicrobials. The factors that influenced dairy farmers' decisions to use antimicrobials for their cattle were veterinarian advice (100%), para-veterinarian advice (96.3%), peer influence (31.2%), and previous experience of using antimicrobials (12.4%). None of them were aware of the drug withdrawal period and followed it. Significant differences in completing the full course of antimicrobial treatment as prescribed have been observed with increasing levels of education (p < 0.001). Conclusion: Despite the wide usage of antimicrobials in dairy farms, there was a significant lack of knowledge among dairy farmers regarding antimicrobials and AMR. The study emphasizes the need for targeted educational interventions to improve farmers' understanding of antimicrobial use and resistance, promote responsible practices, and enhance animal health management. [J Adv Vet Anim Res 2025; 12(2.000): 445-453]

Objective: This study aimed to assess the impact of different rearing site zones with varying temperature– humidity index (THI) on the metabolic regulation of lactating local dairy cows. Materials and Methods: Forty local dairy cows were used in this study, consisting of 20 in rearing sites with THI 66–70 (Pangalengan) and 78–82 (Sumedang), at 950 and 550 m above sea level, respectively. Basal rations were given every morning and evening, consisting of forage and concentrate. Temperature and humidity were recorded daily to determine the average daily THI. Blood samples in both groups of experimental animals were collected according to standard procedures every month during the 4 months of the experiment. Blood analysis followed procedures based on protocols from KIT Randox (UK), using a spectrophotometer. Results: Lipid activity and regulation appeared higher (p < 0.05) in the group of lactating dairy cows kept at THI comfort zone 66–70 than at THI slight stress zone (78–82). Similarly, blood lipid levels were better (p < 0.05) in the cows in the comfort zone (66–70). Conclusion: The study's results on the impact of the rearing zone of lactating local dairy cows appeared to affect the modulation of lipids in the body. Lipogenesis regulation and metabolism showed higher activity in the group of dairy cows reared in the comfort zone (THI = 66–70) compared to the group of dairy cows reared in the discomfort zone. [J Adv Vet Anim Res 2025; 12(2.000): 661-667]