Physico-chemical parameters of fresh milt and mitochondrial activity of fresh spermatozoa of the endangered golden mahseer, Tor putitora (Hamilton) were investigated. Cryopreservation and fertility evaluation of cryopreserved-thawed spermatozoa of T. putitora were carried out. The concentration of spermatozoa and the spermatocrit values of the fresh milt were estimated to be 3.96±0.12 x 107 spermatozoa/ml and 70.00±1.53% respectively. The motility percentage of spermatozoa in the fresh milt was estimated to be 94.84±0.43. The elemental composition analysis revealed that the concentration of K+ was higher i.e., 14.13±0.32 mg/l when compared to that of Na+, Ca+, Mg+ and Zn+. The concentration of total reducing sugars and total proteins were estimated to be 51.79±0.47mg/100 ml and 40.57±0.75 mg/100ml respectively. The absorbance value of the fresh spermatozoa in Sperm Mitochnodrial Activity Index (SMAI) assessment by Nitro Blue Tetrazolium (NBT) assay was estimated to be 0.33±0.012. The optimum sperm: egg ratio was estimated to be 3.96 ±0.12 x 104+ no./egg. Hatching percentage of 45.97±1.72 was recorded for the cryopreserved-thawed  spermatozoa while the fresh milt control recorded a hatching percentage of 73.10±0.82.

 The experiment was conducted to assess the effect of replacement of maize by wheat bran on growth and feed conversion efficiency in Large White Yorkshire (LWY) pigs. Thirty weaned piglets were randomly divided into three groups and were allotted to the three dietary treatments, T1 (control ration as per NRC, 1998), T2 (50 per cent of maize of control ration replaced by wheat bran) and T3 (100 per cent of maize of control ration replaced by wheat bran) and maintained for 70 days. The daily feed intake, fortnightly body weight were recorded and average daily gain and feed conversion efficiency were calculated. The average daily gain and feed conversion efficiency were 813.00, 769.86, 678.43 g and 2.80, 2.88, 3.08, respectively for three dietary treatments. There was no significant difference in the performance between pigs belongs to T1 and T2 treatments but had higher average daily gain and feed conversion efficiency than that of T3 treatment. This study reveals that pigs fed diet with 50 per cent of maize replaced by wheat bran performed in a similar way to that of control fed group and was better than those fed diet with 100 per cent replacement of maize by wheat bran.

The present study was undertaken to induce gonadal maturation of Mrigal, Cirrhinus mrigala using Human Chorionic Gonadotropin (HCG) implants in captive conditions. The male and female fishes of C. mrigala were implanted intramuscularly with hCG implants at the dosage of 1000 IU/kg body weight, to study the effect of hCG hormone. Hormone implants were given once in two months during the experimental period of February to June. Histological observation in the ovary of C. mrigala revealed the presence of four types of growing oocytes namely perinucleolar, previtellogenic, vitellogenic and hydrated oocytes. Histological observation of the testis of C. mrigala showed the presence of five types of spermatogenic cell types namely spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa. Hence, the present study recommends that hCG implants can successfully be used to induce sustained maturation of C. mrigala in captivity even during off season. 

The identification and development of new and effective alternatives to antibiotics that do not hinder productivity assumes paramount importance in the context of antimicrobial resistance and food safety. In recent years, phytobiotics have been used as natural growth promoters in ruminants, poultry and swine, to improve growth and health. A wide variety of herbs, spices and essential oils have been used individually or as blends in poultry for their potential application as alternative growth promoters. In nursery and growing pigs supplemented with phytobiotics, intestinal pathogens were inhibited and beneficial microbial population that contributes to improving digestion and absorption of nutrients were increased resulting in increased weight gain and decrease in diarrheal syndrome. The inclusion of essential oils in the diet of finishing pigs, improved the productive yield and carcass characteristics. It also reduced the incidence of therapeutic treatment and mortality. Natural plant products have the potential to improve rumen fermentation, reduce loss of feed energy, improve animal health and productivity, increase animal lifetime performance, and reduce greenhouse gases production in ruminants. Phytobiotics with wide range of activities, is projected as a feed additive for higher market growth compared to other alternatives to antibiotics in the feed industry.

The current study was designed to evaluate the influence of three different ground vegetable oil seeds on various physico-chemical, microbiological and sensory attributes of chicken sausages from spent broiler breeder hen stored under frozen (-18±1°C) condition. Chicken sausages were incorporated with ground poppy, sesame and peanut seeds each at 10 % level separately as three treatments and were analyzed for their keeping quality along with high fat control. The mean values of FFA, TBARS, tyrosine values, standard plate count, psychrophilic count and yeast and moulds counts were significantly (P<0.05) influenced by formulation during frozen storage and values increased significantly (P<0.05) throughout the storage period in all chicken sausages. Chicken sausages with ground sesame scored lowest standard plate counts (5.66 log CFU/g) than any others after 180 days of storage at 18±1°C. Coli forms, lacto bacillus and anaerobic counts could not be detected during entire storage period. Scores for all sensory attributes decreased significantly (P<0.05) during storage in all treatments irrespective of formulation. Study proved that, the three ground vegetable oil seeds were effective by delaying the lipid oxidation, microbial growth and preserving sensory quality during frozen storage and among them ground sesame seed was more potent to be a better preservative.

A study was planned to analyze the changes in livestock population growth rates and its trends in Tiruvarur district. Tiruvarur shares 2.94 per cent of goat and 2.1 per cent of cattle population to the respective population of Tamil Nadu. The remaining livestock species (buffalo, sheep, pig and poultry) including poultry share of Tiruvarur to states respective species population was negligible, contributing less than one per cent. Recent 20th census, evinced a positive growth rate with highest favourable growth rate of 64.65 per cent for poultry, followed by 2.3 per cent in cattle and 1.44 per cent for goat population of Tiruvarur. Negative growth rates were recorded for other species, which was found to be similar to the state population growth trend. Tiruvarur had a decrease in total livestock population and species-wise too showing negative growth rates between inter census period. Percentage population change over from the base year was 3.23% for goat whereas this was on negative trend for all other species. Analyzing the compositional share of livestock population, goat had the highest share with 53.03±2.62 per cent. All other livestock showed a decreasing trend from 16th to 20th census in Tiruvarur livestock population change. Inadequate infrastructure facilities (77.3%) topped among the five constraints analysed, followed by non-availability of feeds and fodders, mortality and environmental factors. Least constraint was found to be marketing facility. It can be concluded that increasing demand for goat meat, which fetch high remunerative price along with easy managemental practices and favorable conducive environment than other livestock, makes goat farming a highly suitable farming system for Tiruvarur.

A total of 203 blood samples were collected along with data on age at first calving from Murrah / graded Murrah buffaloes maintained at different organized farms. Genomic DNA isolation and Polymerase Chain Reaction (PCR) were performed to amplify the PCR products and were digested with HpaI restriction enzymes overnight. Population genetic indices was calculated based on basic statistical tools. At the MTNR1A / HpaI locus, three genotypes viz., CC, CT and TT identified, with frequencies of 0.225, 0.505 and 0.270 respectively. The allele frequencies of C and T alleles were 0.478 and 0.522 respectively. The overall means of age at first calving was 1385.76 ± 24.31 days. Individuals with the CT and TT genotypes (1356.78 ± 33.03 and 1378.38 ± 37.66 days) had shorter age at first calving than CC genotype (1422.14 ± 53.01 days) and the differences were statistically significant (P<0.05).

A study was conducted to evaluate the biomass yield and proximate composition of biomass from the natural pastures of Madras Red sheep. Eight villages were selected from the habitat of Madras Red sheep and 40 samples each during  rainy and summer season were collected to assess the biomass yield and proximate composition. The study showed that biomass yield and proximate composition were higher during the rainy season during which these sheep completed their breeding, reproduction and lactation. The total dry matter content was higher during the summer season which was able to meet maintenance requirement of sheep during these months. Farmers managed these animals without any supplementary feeding, but during summer the animals were let in the harvested fields where they had access to crop residues for grazing.

Phylogenetic analysis of organisms not only offers crucial details on the genesis and evolution of genes, genomes, and species, but also helps with ecological and behavioural research while forecasting the magnitude and direction of future evolutionary trends in living things. In the present study, cytochrome b gene sequence of mitochondrial DNA obtained from captive Bengal tiger (Panthera tigris) from the Arignar Anna Zoological Park, Vandalur, was phylogenetically analysed with reference sequences of cytochrome b gene of fourteen sequences of the genus Panthera obtained from NCBI gen bank database. The similarities and differences between the different sequences were studied and the use of phylogenetic analysis as a forensic tool for investigation was discussed. The phylogenetic tool can also be used for pedigree checking where the parentage details are maintained and the place of origin of the species can be traced in case of samples seized during animal poaching.

A proper care, prompt reporting and immediate surgical inervention will save the life of the animal. A four year old pluriparous doe was presented with the history of dog bitten wound with eviscerated mass from the injured site at the left lateral aspect of the abdomen. The goat was kept on lateral recumbancy, on clinical examination revealed eviscerated rumen without any lacerations. Surgical area was prepared aseptically, under Inj. Xylazine as premedication and Inj. ketamine and inj. diazepam as induction anaesthesia reconstructive surgery was performed. Rumenotomy was performed to reduce the content and rumen was closed by the two rows of inversion suture. Peritoneum and transverse abdominus muscle were apposed by inversion suture pattern and oblique abdominus internus and externus muscle were apposed by interrupted suture pattern using catgut no 1. Skin was apposed by interupted suture pattern using cotton thread (no 8). Postoperatively Inj. Tetanus toxoid (5 Lfu), ceftriaxone @ 10 mg per kg and meloxicam @ 0.5 mg per kg were given intramuscularly and the animal was maintained with fluid therapy, antibiotic and analgesic for five days and animal recovered after one week.