The current study utilized 20 postpartum anestrus buffaloes maintained at an organized dairy farm in Vuyyuru, Krishna district to compare the metabolic (glucose), mineral (calcium) and anti-oxidant (superoxide dismutase) levels in ovsynch-48 (n=10, G48) and ovsynch-56 (n=10, G56) postpartum anestrus graded Murrah buffaloes. G48 group of buffaloes received 20 µg of GnRH on Day 0, followed by 500 µg PGF2 on Day 7, a final dose of 20 µg of GnRH was given 48 hours after PGF2α on Day 9. Whereas, G56 group of buffaloes received 20 µg of GnRH on Day 0, followed by 500 µg of PGF2α on Day 7, a final dose of 20 µg GnRH was given 56 hours after PGF2α on Day 9. In both groups, FTAI was performed at 16-21 hours interval after observing estrus. Results revealed that on different days of treatment, serum glucose and calcium levels did not differ significantly (P>0.05) in both treatment groups; however, Superoxide dismutase levels varied significantly (P<0.05) on different days of treatment in both groups. The results of the study parameters could play pivotal role in reproductive function and may provide insights into the underlying mechanism of anestrus.

The present study was aimed to assess the efficacy of oocyte retrieval through transvaginal ovum pick-up (OPU) in Ongole (Bos indicus) cows. Cows (n=18) were divided in to two equal groups; cows in group 1 cows were subjected to two OPU sessions (OPU1 and OPU2) at 96 h interval irrespective of the stage of estrus cycle. Cows of group 2 were subjected to FSH pre-stimulation before OPU 1 followed by OPU 2, 96h later. Thus, a total of 36 OPU sessions were performed on 18 animals.  The number of follicles available for aspiration (17.89 ± 1.78 vs 27.06 ± 1.75), number of medium (4 – < 8 mm; 4.11 ± 0.69 vs 16.00 ± 1.76) and large follicles (≥ 8mm; 1.06 ± 0.23 vs 6.33 ± 0.79), follicles aspirated (11.95 ± 1.42 vs 17.45 ± 2.07), COCs recovered (5.72 ± 0.78 vs 10.06 ± 1.78), and viable COCs collected (4.23 ± 0.67vs 8.34 ± 1.79) were significantly higher in group 2 than in group 1.The mean number of follicles aspirated, the mean oocyte recovery and the viable oocytes collected were significantly higher at OPU 1 in both the groups than at OPU 2. It was concluded that pre-treatment with FSH increased the OPU efficiency in terms of oocyte yield and viable oocytes collected. OPU 2 performed at shorter interval after OPU 1 is not advantageous due to limited number of follicles available for aspiration at OPU 2 and consequently, reduced oocyte recovery.

The present study was aimed to investigate the presence of Escherichia coli (E.coli) in raw chicken meat samples collected from retail shops, as well as the biofilm-forming ability of field isolates, and to characterize different adhesion genes. Out of 20 chicken meat samples, 17 (85%) were positive for E. coli. Fifteen E. coli strains were characterized by PCR using the 16S rDNA primers and all the isolates were positive which confirmed that all the isolates were E.coli. Out of the 15 confirmed E.coli field isolates which were subjected to biofilm-forming assay, 46% of them were found to be strong biofilm producers. While all the isolates were screened for the presence of adhesion genes viz. luxS, csgA, fimH, fimA, and papC, the adhesion gene luxS was detected in all the strains (100%). The other adhesion genes csgA, fimH and fimA were detected in 93%, 93%, and 73% of the isolates, respectively. The E. coli field isolates were screened blaTEM gene was detected in only four strains, which was categorized under strong biofilm producers. This study demonstrated the presence of biofilm forming E. coli in the raw chicken meat samples as contaminants, causing spoilage and potentially posing risk to consumer’s health and safety.

The present study was conducted to study the effect of maize (Zea mays) cob on replacing paddy straw in the Total Mixed Ration (TMR). Twelve cross bred calves of about 5 to 8 months of age with body weight ranging from 41 to 79 kg were divided into two groups of six each in completely randomized design. Five complete diets were prepared (TMR1 to TMR5) using maize cobs at the level of 0%, 25%, 50%, 75% and 100% replacement of paddy straw in the diets containing 12 % CP and 60 % TDN of 50:50 concentrate to roughage ratio. There was significant (P<0.01) differences among the diets in OM, CF, NFE, TA, AIA, NDF, ADF, Lignin, Hemicellulose and Cellulose contents. In vitro rumen fermentation study showed significantly (P<0.01) higher total gas (ml/200 mg/48 h), in vitro dry matter and organic matter degradability in maize cob based ration than paddy straw contained ration (51.17 vs 36.00; 62.87 vs 57.25; 64.80 vs 59.93). Paddy straw (100%) based diet as control ration and maize cob (100 % replacement of paddy straw) based diet as treatment ration fed to growing calves for sixty days in growth trial. A seven day digestion trial was conducted in the middle of the experiment. The digestibility (%) of DM, OM, CP, EE, CF and NFE were significantly (P<0.01) higher in the maize cob based diet fed group than paddy straw based diet fed group. The average body weight gain (kg) and FCR (kg DMI/ kg gain) were significantly (P<0.05) higher in maize cob fed animals (19.47; 6.10) than paddy straw fed animals (16.02; 7.34). The feed cost per unit of weight gain in calves fed paddy straw based diet was numerically higher (30.78 %) than calves fed on maize cob based ration. It could be concluded that maize cob based ration could replace paddy straw at 100 % level in total mixed ration without affecting feed intake and nutrient digestibility and may also improve the body weight gain and reduce feed cost in growing calves.

Avian species reproduce sexually by the fusion of male and female gamete spermatogonia and oogonia-produced by differentiated testes and ovaries. Fertilization is internal and all birds are oviparous. Reproduction in birds entirely varies from other animals. The two essential reproductive organs ere the ovary and oviduct which were involved in the synthesis of egg in adult laying hen. Gross and morphometrical studies were carried out on the female reproductive tract in twelve Siruvidai chicken of which six at laying phase and six at broodiness phase. The birds were reared in Poultry Research Station, Madhavaram, Chennai-51. In laying phase, the left ovary showed mature ovarian follicles whereas the ovary during broodiness phase was in the form of a bunch of grapes with no hierarchical follicles. There was no significant difference in the body weight of the bird during laying and broodiness. The mean length, weight and width of ovary and oviduct had a highly significant difference during laying and broodiness phase. Gross morphology revealed that only the left female reproductive tract was well-developed both during laying and broodiness phase and the morphometric data analyzed will provide a baseline for subsequent studies and also for comparison with other avian species.

The calves presented with abdominal affections requiring surgical interventions were randomly divided into group I and group II with seven animals in each group.  All the animals were premedicated with dexmedetomidine at 2 µg/kg body weight, pentazocine at 1 mg/kg body weight and midazolam at 0.2 mg/kg body weight intravenously. In group I and group II, anaesthetic induction was carried out with propofol at 2 mg/kg body weight and etomidate at 0.25 mg/kg body weight intravenously, respectively. Both the groups were maintained in Isoflurane anaesthesia with modified oro-endotracheal tube. The cardiovascular parameters such as heart rate, mean arterial blood pressure, systolic and diastolic blood pressure were monitored during different stages of anaesthesia in both the groups. In both the groups, a significant decrease in heart rate was noticed after premedication and anaesthetic induction. A Significant increase was noticed in systolic blood pressure values after anaesthetic induction and 10th minute of anaesthesia in group I and a highly significant increase was noticed after induction up to 10 minutes in group II.  A highly significant increase in diastolic blood pressure was noticed after induction and 10th minute in group I, whereas significant increase was noticed in mean arterial pressure after induction and 15th minute of anaesthesia in group II animals. The cardiovascular parameters recorded in the present study concluded etomidate produced better cardiovascular stability and minimal respiratory depression compared to propofol throughout the anaesthetic period.

This case report details the episiotomy approach employed in managing vulval hypoplasia in a two year old Miniature Pomeranian bitch. The animal was presented to the Teaching Veterinary Clinical Complex, Veterinary Gynaecology and Obstetrical Unit, Madras Veterinary College, displaying a history of greenish black discharge and continuous straining for two hours. The animal was bred before 64 days. Physical examination revealed a small vaginal opening, measuring approximately 1.5 cm in length. Digital examination indicated fetal hind limbs and tail extending into the vaginal passage, while all other vital parameters were within the normal range. Attempts to induce uterine contractions using Inj. Oxytocin and 10 % Calcium gluconate in 5 % DNS proved unsuccessful. Therefore, under local anesthesia (2 % Lignocaine injection), median episiotomy was made and a single live male puppy was successfully delivered with mild traction. Closure of the incision site was accomplished using PGA 1/0 with a simple interrupted suture pattern. The patient had uneventful recovery after 5 day treatment regimen, involving a single dose of subcutaneous Inj. Cefovecin (antibiotic) and anti-inflammatory drugs, along with meticulous wound management.

A case of fetal mummification among one of the quadruplet kids was reported in a fourth parity Kanni adu doe.

The present study was aimed to know the chronic hepatitis with abdominal effusion in a beagle dog and its therapeutic management. A 2.5 year old male beagle was presented to Madras Veterinary College teaching Hospital, madras veterinary college, Chennai with the history of inappetence, abdominal distension for past 10 days and the clinical signs of inappetence, fever and palpablelymph node and congested mucous membrane. On Physical examination, dog showed respiratory distress and dull. Dog was subjected for radiography examination, haematology, biochemistry parameters and ultrasound examination. On first day of examination, dog was treated with Lasix at the dose rate of 3 mg/kg body weight, antibiotics and Tribivet injection. Hematology and biochemical values revealed neutrophilia, elevated alkaline phosphatase, total bilirubin, direct bilirubin, and decreased total protein and albumin. The lateral view radiography of abdomen revealed fluid shadow and ultrasound examination revealed that chronic hepatitis with moderate abdominal effusion. In case of clinical suspicion of hepatitis an ultrasonographic liver scan can be of diagnostic aid and the finding of an altered structure can be indicative of a liver problem. In many cases of chronic hepatitis may not be associated with ultrasound abnormalities but ascites can be easily identified. Dog was treated with antibiotic cefotaxime at the dose rate of 25 mg/Kg body weight and Lasix at the dose rate of 3 mg/Kg body weight with liver supplement of Lysibin which contain silymarin and supplement with Astymin syrup for 3 weeks. The animal was reviewed after three weeks of treatment which was subjected for haeamtology, biochemistry and ultrasound examination which revealed there was improvement in the blood parameters with decreased total protein and normal level of Alkaline phosphatase and bilirubin level and ultrasound examination revealed normal study of abdominal effusion and liver echogenicity and dog had normal  appetite and normal  vital parameters. In this case hepatitis may be due to any viral etiology like adenovirus that may cause hepatitis with effusion that may be respond to treatment and diuretics and brought normal parameters of vital in dog. Chronic hepatitis with effusion may be further confirmed by Liver biopsy and molecular diagnosis.

Reducing crude protein (CP) level in the feed without altering the amino acid contents by supplementing synthetic amino acids to reduce nitrogen excretion, adding ofexogenous protease enzyme infeed to improve nitrogen utilization and treating litter with the culture ofMethylomonas methanica (MM) to prevent methane emission from litter are the proven methods in commercial poultry production, especially in broiler farming to protect the environment. However, the efficacy of these nitrogen emission reduction strategies on the performance and economics of guinea fowl in intensive production has not been studied adequately. Therefore, a biological experiment was conducted with high protein (HP) diets with (Tl) or without (T2) fish meal (FM) and low protein (LP) diets added with exogenous protease enzyme with (T3) or without (T4) FM. The levels of critical amino acids in all the feed were balanced with the supplementation of synthetic amino acids. These treatments contained six replicates of each with 15-day-old keets. At the start of ninth week, the litter of three replicates from each treatment was sprayed with MM culture additionally, forming four more treatment groups, from T5 to T8 respectively. The results of the study indicated that replacing FM with plant protein sources did not affect body weight. The overall trend revealed that the reduction of CP by 2% had a negative (P<0.05) impact on body weight; while, the addition of protease in feed had no notable effect. The feed consumption was not affected by the withdrawal of FM or reducing CP level by 2% ordietary protease supplementation or MM litter treatment. The feed efficiency was negatively impacted (P<0.05) by withdrawal ofFMin high and low protein diets; -while, feed efficiency -was not improved by protease supplementation in LP diets. The level of CP or -withdrawal FM or protease enzyme supplementation in the ration did not affect livability. The MM litter treatment did not affect gro-wth rate, feed consumption, feed efficiency and livability of guinea fowls. The results indicated that the benefit-cost ratio could be improved in guinea fowls by using low CP feed.