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Comparison between ultrasound images of the dog brain with and without the calvaria and its correlation with real anatomy | Comparação entre imagens ultrassonográficas do cérebro do cão com e sem a calota craniana e sua correlação com a anatomia real
2013
Carla Aparecida Batista Lorigados | Ana Carolina Brandão Fonseca Pinto
<p>Bones are believed to be an effective barrier to obtain sonographic images. In fact, the large difference between acoustic impedances of surfaces of the soft and bone tissues generates significant image artifacts. However, transmission of the ultrasound beam depends on bone thickness and structure. Therefore, the temporal bone has been used as an acoustic window to access the brain of adult patients with ultrasonography. The purpose of this study was to assess the brain of adult dogs using ultrasonography with and without bone interposition, compare the images, and correlate them with the brain anatomy. Ten mesaticephalic adult dogs were used, and the ultrasound examination was performed through the magnum orifice and on the temporal, lateral parietal, and frontal bones. A small craniotomy was performed in the frontal bone for examination without bone interposition. We were able to acquire images of the brain with bone interposition. However, resolution of these images was lower than the ones obtained by craniotomy. Important anatomical structures were identified. Regarding the correlation and the wide availability of ultrasound equipment, it was concluded that ultrasound can be used as a tool for monitoring expansive intracranial lesions or in intraoperative procedures.</p> | <!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings> </xml><![endif]--><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Calibri','sans-serif'; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: PT-BR; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">Os ossos são tidos como uma barreira intransponível para obtenção de imagens sonográficas, pois a grande diferença entre as impedâncias acústicas das superfícies de tecidos moles e ósseos gera importantes artefatos de reflexão, contudo, a transmissão do feixe ultrassônico depende da espessura e da estrutura óssea e neste caso o osso temporal tem sido usado como uma janela acústica para o acesso, por ultrassonografia, do encéfalo de pacientes adultos. O presente trabalho examinou o encéfalo de cães adultos com o emprego da ultrassonografia com e sem a interposição do crânio, comparou as imagens obtidas e as correlacionou com a anatomia encefálica. Foram examinados dez cães adultos, mesaticefálicos. O exame ultrassonográfico foi realizado através do orifício magno e dos ossos temporal, parietal (porção lateral) e frontal. No exame sem a interposição óssea foi realizada uma pequena craniotomia no osso frontal. As imagens do encéfalo com a interposição óssea apresentaram resolução inferior às obtidas por craniotomia. Importantes estruturas anatômicas foram identificadas. Devido à correlação e a sua ampla disponibilidade, concluiu-se que o aparelho de ultrassom pode ser usado para o acompanhamento de lesões intracranianas expansivas, bem como em procedimentos intraoperatórios.</span><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves/> <w:TrackFormatting/> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF/> <w:LidThemeOther>PT-BR</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> <w:SplitPgBreakAndParaMark/> <w:EnableOpenTypeKerning/> <w:DontFlipMirrorIndents/> <w:OverrideTableStyleHps/> 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Показать больше [+] Меньше [-]Similaridade genética entre APEC e cepas de Escherichia coli isoladas de Guaruba guarouba em um estudo com psitacídeos hígidos de cativeiro | Genetic similarity between APEC and Escherichia coli strains isolated from Guaruba guarouba in a survey on healthy captive psittacine birds
2013
Fabíola Eloisa Setim Prioste | Marcos Paulo Vieira Cunha | Rodrigo Hidalgo Fricielle Teixeira | Ticiana Zwargg | Rosely Gioia Di-Chiacchio | Priscilla Anne Melville | Nilson Roberto Benites | Juliana Sinhorini | Eliana Reiko Matushima | Terezinha Knöbl
<!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings> </xml><![endif]--><p class="MsoNormal">O papel dos psitacídeos como reservatório de Escherichia coli patogênicas para aves (APEC) não é conhecido, mas será útil para a compreensão da interface humano-animal, uma vez que a microbiota entérica destas aves é composta por bactérias Gram-positivas. O objetivo deste estudo foi identificar a presença de APEC em fezes de Guaruba guarouba clinicamente saudáveis. Para isso, foram isoladas e analisadas E. coli presentes em fezes cloacais coletadas de 87 psitacídeos, alojados em seis zoológicos, três criatórios comerciais e um criatório conservacionista. Das 87 aves examinadas, 46 (52,87%) apresentaram E. coli nas fezes. A presença de oito genes de virulência foi determinada pela reação em cadeia pela polimerase (PCR): irp2, iucD, iss, vat, cvi/cva, tsh, astA, e papC, e 29 (63,04%) dos 46 isolados foram positivos para pelo menos um dos oito genes estudados. A frequência dos genes de virulência observada nos isolados de E. coli foi 32.6% (15/46) irp2, 26% (12/46) iucD, 19.5% iss (9/46), 17.4% vat (8/46), 17.4% cvi/cva (8/46), 8.7% tsh (4/46), 4.4% astA (2/46) e 0% papC (0/46). Os isolados foram agrupados em 13 perfis genotípicos de acordo com combinações de genes de virulência, mas apenas duas amostras foram classificadas como APEC, com o perfil iuc, iss, cvi/cva, irp + e iuc, iss, cvi/cva, irp, tsh, iuc, iss, +. Este estudo revela a presença de APEC em aves de cativeiro (G. guarouba) clinicamente saudáveis, sugerindo que estes psitacídeos possam atuar como reservatórios de micro-organismos patogênicos. 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Name="TOC Heading"/> </w:LatentStyles> </xml><![endif]--><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;} </style> <![endif]--></p><p class="MsoNormal"><span style="mso-ansi-language: EN-US;" lang="EN-US">The role of psittacine birds as a reservoir of avian pathogenic Escherichia coli (APEC) is not known but would be helpful in understanding the human – animal interface, since the enteric microbiota of these birds consists of Gram positive bacteria. The aim of this study was to identify the presence of APEC in feces of clinically healthy Guaruba guarouba. To do this, we isolated and analyzed E. coli from cloacal fecal samples taken from 87 psittacine birds from six zoologic parks, three commercial breeders and one conservation breeder. Of the 87 birds examined, 46 (52.87%) presented E. coli in feces. The presence of the following eight virulence genes was determined by the polymerase chain reaction (PCR): irp2, iucD, iss, vat, cvi/cva, tsh, astA, and papC, and 29 (63.04%) of 46 E. coli isolates tested were positive at least one of the eight genes studied. The frequency of virulence genes observed in isolates of E. coli were 32.6% (15/46) irp2, 26% (12/46) iucD, 19.5% iss (9/46), 17.4% vat (8/46), 17.4% cvi/cva (8/46), 8.7% tsh (4/46), 4.4% astA (2/46) and 0% papC (0/46). The isolates were grouped in 13 genotypic profiles according to virulence gene combinations, but only 2 isolates were classified as APEC, with the pattern iuc, iss, cvi/cva, irp + and iuc, iss, cvi/cva, irp, tsh, vat +. This study reveals the presence of APEC in clinically healthy captive G. guarouba, suggesting that these psittacine birds may act as reservoir for pathogenic microorganisms. Epidemiological studies are needed to determine the relevance of this species as a reservoir and the implications for conservation of endangered species G. guarouba.</span></p>
Показать больше [+] Меньше [-]Faillure in the effect of the analogue (hCG) of luteinizing hormone on the luteal angiogenesis in rats (Rattus novergicus) | Ausência de efeito do análogo (hCG) do hormônio luteinizante na angiogênese luteal em ratas (Rattus novergicus)
2013
Diego Gonçalves Silva | Diego Marcondes Guerra | Vinicius José Moreira Nogueira | Mariana Andrade Torres | Diego Feitosa Leal | Carlos Henrique Cabral Viana | Caroline Martins
<!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings> </xml><![endif]--><p class="MsoNormal"><span style="mso-ansi-language: EN-US;" lang="EN-US">The knowledge of the mechanisms that affect the control of the ovarian activity is essential for the success of reproduction biotechnologies. Although a number of studies have been carried out in which the luteinizing hormone (LH) was used to control the ovarian activity, little is known about its influence in the morphology and vascular formation of the corpus luteum, aiming to increase the local blood flow. Thus, the objective of the present experiment was the quantification of the vascular density of corpora lutea (Cls) in animals treated with human chorionic gonadotropin (hCG) just after ovulation. Therefore, eighteen wistar rats were used in this experiment. Eight rats in the treated group and ten rats in the control group. Corpora lutea were divided into two groups: group (A) treated with hCG in the following morning after copulation, and group (B) control animals which received an injection of 0.9% sodium chloride solution. Ovaries from each group were used for preparation of histological sections for vascular density qualification. No statistical significance was found between the two groups tested.</span></p><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves/> <w:TrackFormatting/> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF/> <w:LidThemeOther>PT-BR</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <w:DontGrowAutofit/> <w:SplitPgBreakAndParaMark/> <w:EnableOpenTypeKerning/> <w:DontFlipMirrorIndents/> <w:OverrideTableStyleHps/> </w:Compatibility> <m:mathPr> <m:mathFont m:val="Cambria Math"/> <m:brkBin m:val="before"/> <m:brkBinSub m:val="--"/> <m:smallFrac 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line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;} </style> <![endif]--> | <!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings> </xml><![endif]--><p class="MsoNormal">A compreensão dos mecanismos de controle da atividade ovariana é necessária para o sucesso das biotecnologias da reprodução. Embora existam inúmeros trabalhos a respeito da aplicação do hormônio luteinizante (LH) na função ovariana, pouco se sabe sobre a sua influência na morfologia e formação da vasculatura do corpo lúteo (CL). Diante disto, o presente projeto teve como objetivo a quantificação da densidade vascular dos CLs de animais tratados com Gonadotrofina Corionica Humana (hCG) após a ovulação. Para tanto, foram utilizados ratas wistar, cujos CLs foram divididos em dois grupos: (A) tratado com hCG na manhã seguinte a cópula e (B) controle (solução fisiológica a 0,9 % de NaCl). Foram confeccionadas lâminas dos ovários dos animais para a quantificação da densidade vascular. 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Показать больше [+] Меньше [-]Caracterização genética do vírus da diarreia bovina detectada em bovinos do Sul de Minas Gerais, Brasil, persistentemente infectados | Genetic characterization of bovine viral diarrhoea virus detected in persistently infected cattle in Southern Minas Gerais, Brazil
2013
Fabio Carvalho Dias | Kerlei Cristina Médici | Ricardo Luiz Moro de Sousa | Amauri Alcindo Alfieri | Samir Issa Samara
<p>Isolados do vírus da diarreia viral bovina (BVDV) detectados em amostras de soro sanguíneo de dois animais persistentemente infectados (PI), identificados num rebanho bovino localizado na região sul do Estado de Minas Gerais, Brasil, foram submetidos à caracterização genética através do sequenciamento parcial de nucleotídeos da região 5’UTR do genoma viral. Os isolados foram caracterizados como pertencentes ao genótipo BVDV-1, subgenótipo BVDV-1b. Os resultados do presente estudo sugerem o BVDV-1b como um agente de importância na ocorrência da diarreia viral bovina (BVD) nos rebanhos da região. Ademais, a caracterização genotípica dos isolados do BVDV contribui para a melhor compreensão da epidemiologia da enfermidade, pois a variabilidade genética do BVDV interfere nos testes sorológicos e também possui implicações na utilização de vacinas, cuja maioria é produzida apenas com estirpes de referência do BVDV, requerendo, portanto, investigações sobre a diversidade genética do BVDV existente no Brasil para o aprimoramento das medidas de prevenção e controle da enfermidade no país.</p> | Isolates of bovine viral diarrhoea virus (BVDV) detected in serum samples of two persistently infected animals (PI) identified in a herd located in the southern state of Minas Gerais, Brazil, underwent genetic characterization trough partial nucleotide sequencing and analysis of the 5’ Untranslated Region (5’UTR) of the viral genome. The isolates were characterized as belonging to genotype BVDV-1, subgenotype BVDV-1b. The results of this study suggest BVDV-1b as an agent of importance in the occurrence of bovine viral diarrhoea (BVD) in the herds of the region. Moreover, the genotypic characterization of isolates of BVDV helps to better understand the epidemiology of the disease, as the genetic variability of BVDV interferes in the serological tests and has implications for the use of vaccines, whose majority is produced only with reference strains of BVDV. Therefore, the investigation on the genetic diversity of BVDV existing in Brazil is required for the improvement of the disease prevention and control measures
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R. Carareto | M. G. Sousa | P. N. H. Guerrero | D. P. De Paula | C. T. Nishimori | N. Nunes | T. Carvalho
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M. B. F. Silva | C. Carnicelli | A. C. Rezende | L. F. Burgese | M. M. Jericó
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V. S. C. Rodriguez | J. G. Padilha | J. C. Canola
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2013
L. M. G. Silveira | A. Matera | S. R. G. Cortopassi | J. G. Xavier | C. R. A. Ferrigno | F. M. Cunha
O artigo não apresenta resumo.
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V. S. Galeazzi | J. M. Matera | P. F. Castro
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