This study was conducted with the purpose of investigating the distribution of the Activating Transcription Factor 6 (ATF6) and the Nerve Growth Factor (NGF) in the duodenum tissue of diabetic and non-diabetic rats. Eighteen female Sprague dawley rats were randomly divided into three groups as thecontrol, sham and diabetes groups. Routine histological and immunohistochemical methods were appliedon the duodenum tissues collected at the end of the study.Results: It was determined that the villus length measurements showed a statistically significant differencebetween the control and diabetes groups. There was NGF immunoreactivity which was moderate anddiffuse cytoplasmic in the villus intestinalis and muscularis layer in all groups, weak in the crypts andglands in the control and sham groups and moderate and diffuse cytoplasmic in the diabetes group. ATF6immunoreactivity was determined moderate in the villus intestinalis, crypts, glands and muscularis layerin the control and sham groups and strong diffuse cytoplasmic in the diabetes group. It wasdetermined that both NGF and ATF6 immunoreactivity increased in the duodenum tissue of the rats onwhich diabetes was induced experimentally.

This study; It was aimed to compare serum and rumen content, trace element and serum vitamin levels and rumen content in indigestion cattle with healthy animals. Materials and Methods: The study was conducted on a total of 30 cattle, 10 healthy (control group) and 20 indigestion group. Indigestion diagnosis in animals was determined by anamnesis information, clinical and rumen content examination.Hematologically, there was no statistical difference between indigestion and control groups. Serum magnesium (Mg), calcium (Ca), zinc (Zn) levels of the measured trace elements decreased in the group with indigestion (p<0.05), while the levels of cobalt (Co) increased significantly (p<0.01). While there was a significant increase (p<0.5) in cattle with indigestion in rumen content Ca values compared to healthy cattle, serum levels were decreased (p<0.05). A positive correlation (p<0.01) was determined between rumen content levels of indigestion cattle and serum Mg levels of control group and rumen content of control group. In addition, a negative correlation was found between serum Ca and rumen content values of cattle with indigestion. In serum vitamin levels, Vitamin B1 (Vit B1) decreased statistically (p<0.05), while Vitamin B12 (Vit B12) was found to increase non-statistically (p>0.05).As a result, it was concluded that the decrease in serum Mg, Ca and Zn values in indigestion animals is important and these trace elements should be used in treatment.

Indiscriminate use of antibiotics in poultry farms increases the chance of antibiotic resistant and extended-spectrum β-lactamase (ESBL) producing bacteria in Bangladesh. Therefore, the study was under taken to detect ESBL producing Escherichia coli (E. coli) in chickens. Materials and methods: A total of 60 cloacal swab samples (20 from commercial layer, 20 from commercial broiler and 20 from commercial sonali chickens) were collected from Rajshahi district of Bangladesh. The E. coli was isolated from these samples and identified based on cultural, staining, and biochemical characteristics. The disk diffusion method was used to assay the antibiotic resistant/sensitivity patterns of the isolated E. coli. Phenotypc detection of ESBL producing E. coli was also done.The prevalence of E. coli in chickens was 61.67% in Rajshahi district of Bangladesh, where the prevalence was 60%, 60%, and 65% in commercial layer, commercial broiler, and commercial sonali chickens, respectively. The antibiotic sensitivity assay of E. coli isolated from commercial layer chickens showed 100%, 80%, 50%, 40%, and 40% resistant to amoxicillin, tetracycline, cefotaxime, ciprofloxacin, and ceftazidime, respectively. E. coli isolated from commercial broiler chickens showed 100%, 100%, 60%, 50%, and 40% resistant to amoxicillin, tetracycline, cefotaxime, ceftazidime, and ciprofloxacin, respectively. E. coli isolated from commercial sonali chickens showed 90%, 70%, 50%, 50%, and 40% resistant to amoxicillin, tetracycline, cefotaxime, ciprofloxacin, and ceftazidime, respectively. In phenotypic detection, the overall prevalence of ESBL producing E. coli was 43.33%, where 40%, 50%, and 40% in the commercial layer, commercial broiler, and commercial sonali chickens, respectively in Rajshahi district of Bangladesh.These results indicated that chickens are a potential reservoir for ESBL producing E. coli and their antibiotic resistances are obviously significant. These findings will help us to make proper guideline for the treatment, prevention and control of E. coli prevalent in chickens in Bangladesh.

The present study was carried out to investigate the possibility of using different levels of bee pollen and apigenin extract in beef meatballs to evaluate shelf-life, nutritional and quality properties of beef meatballs under different storage conditions. It was determined, using the HPLC method, that bee pollen contains a high level of apigenin under optimal conditions. Bee pollen and apigenin were added to meatballs at different concentrations in five groups. Meatballs were made with control, 1-2% bee pollens/apigenin. Quality and safety evaluation of meatballs were determined by sensory, physicochemical, biochemical and microbiological tests. The analyses were conducted at 1st, 3rd, 7th and 14th days of interval. A statistically significant decrease was found in FFA, POV and TBARS levels of meatballs on different days of storage (p<0.05). When compared to the control group, the bioactive compounds preserved the microbiological and chemical properties of meatballs during frozen storage (14 days).It was concluded that the extracts with 2% bee pollen concentrations can be used as biopreservative agents for meat and meat products.

The aim of the current study was to evaluate the effect of different levels of Curcuma longa on some physiological and biochemical parameters, as well as some egg productive and quality characters and the intestinal microflora of local quail. 240 one-day-old quail were distributed randomly into 4 groups (60 birds/group) with 3 replicates. The groups were as follows: 1st group (control) birds were reared on standard ration, the 2nd, 3rd and 4th groups birds were reared on standard ration supplemented with 3, 6 and 9 gm turmeric/kg ration respectively. The results revealed that the turmeric improve blood picture as represented by the significant increase of RBCs, Hb and PCV% specially in 3rd and 4th treatments. Also, the turmeric reduced significantly triglycerides and cholesterol compared with the control. Curcuma longa treatment improved the intestinal microflora represented by the significant decrease in the number of pathogenic flora (Salmonella and E. coli), and significant increase in the benefit flora (Lactobacillus). On the other hands treatments reduced significantly the age of 1st egg production and enhance the age of 50% egg production. In conclusion, Curcuma longa treatment induced ameliorative effects on some of physiological and productive performance of quail.

Abortion causes substantial economic losses in sheep flocks. In addition to financial compensation, abortion is a sharp aspect of public health.&nbsp;Brucella, Campylobacter, Salmonella and Chlamydia are the most examples of diseases associated with the abortion of ewes. Brucellosis is a zoonotic bacterial infection produced by a variety of species of Brucella. It has a significant economic impact on domestic animals, primarily ewes. The current study investigated Brucella ovis from aborted fetuses and vaginal swab samples collected from sheep flocks in the Sulaimani governorate by the polymerase chain reaction. Thirty-eight aborted fetuses and 70 vaginal swabs were collected from sheep flocks in three districts of Sulaimani governorate (Kalar, Chamchamal and Said Sadiq) from March 2018 to June 2019. The pathogen was identified in clinical specimens using conventional PCR. Brucella ovis was isolated from 26 of 38 aborted fetuses (68.4%) and 21 of 70 vaginal swabs (30&nbsp;%) of aborted ewes. The Brucella ovis gene ompA was sequenced, and phylogenetic analysis of the ompA gene sequences revealed that Brucella ovis isolated established a distinct branch and a lower relationship of Brucella ovis to the Brucella melitensis species. It has been concluded that Brucella ovis is the most pathogenic Brucella species and a major cause of ewe's abortion in the Sulaimani governorate.

&nbsp; This study was conducted to detect the blaCMY-2 gene and qnr genes (qnrA, qnrB and qnrS)in Salmonella isolates from 278 different samples (50 direct milk samples, 50 indirect milksamples, 50 feces samples, 50 teat swab samples, 28 manual milk swabs and 50 stoolsamples) in Basrah province. The results showed that the percentage of Salmonella isolatesin the samples was 6.1% by using API system and by PCR technique for identification. Thehighest resistance to Salmonella isolates were found against chloramphenicol and rifampin(100%). While all isolates were sensitive to ciprofloxacin . The use of plasmid treatment(Plasmid curing) by temperature method showed that 41.1% of total Salmonella isolateswere associated with antimicrobial resistance of the plasmid. Plasmid analysis by moleculardetection revealed that 11 isolates (64.7%) was positivity for blaCMY-2 while the qnrquinolone gene (A, B and S) was not detected in the isolates

The study's objective is to evaluate the enhanced effect of the ghrelin on the body weight and thyroid hormones in male rats after inducing hyperthyroidism by L&ndash;thyroxin. The rats (95 males) were split into two groups. The first group consisted of 25 male rats that were given normal saline for 30 days S.C and set as a control group. While the remainder of the animals were given levothyroxine 500g/kg subcutaneously for 30 days to induce hyperthyroidism. after induction the divided into 4 groups as followe ,the first one was the control group that mentioned previously, the second group was male rats were given normal saline for 30 days S.C, the third group was male rats were given ghrelin at a dose of (0.5nmol/100&mu;l saline) for 30 days S.C, and the fourth group was male rats (1nmol/100&mu;l saline) for 30 days S.C.&nbsp;&nbsp; The fifth group consisted of hyperthyroidism male rats that was given ghrelin at a dosage of (2nmol/100&mu;l saline) for 30 days S.C. The results of final weight and weight gain are presented showed no significant difference in initial weight within all groups that were observed, while a significant decrease in final body weight in hyperthyroidism group compared with control group. On the other hand, the results revealed a significant decrease in body weight gain in male rats have hyperthyroidism compared with the control group. While the results observed a significant increase final body weight and body weight gain in all treated rats with ghrelin as compared with hyperthyroidism group. On other hand, the effect of hyperthyroidism on serum TSH, T3, and T4 concentrations revealed that the hyperthyroidism group had a significant rise in serum T3 and T4 concentrations when compared to the control group. While no significant drop in serum TSH concentration was observed in all hyperthyroidism groups handled with ghrelin (0.5, 1 and 2 nmol) as compared to the hyperthyroidism group, a significant decrease in serum T3 and T4 level was observed in all hyperthyroidism groups treated with ghrelin in comparison to the group of hyperthyroidism. Ghrelin peptide hormone and it has been shown to have potential effects on the body weight and thyroid hormone.

No abstract available

OBJECTIVE To compare intraoperative and short-term postoperative variables pertaining to laparoscopic ovariectomy (LapOVE) and open ovariectomy (OVE) in rabbits (Oryctolagus cuniculus). ANIMALS Twelve 4− to 5-month-old female New Zealand White rabbits. PROCEDURES Rabbits were randomly assigned to undergo LapOVE (n = 6) or OVE (6), with a vessel-sealing device used to seal and transect the ovarian pedicles. Laparoscopic ovariectomy was performed with a 3-port approach. Variables were measured during surgery (surgery and anesthesia times and incision lengths) and for up to 7 days after surgery (food consumption, feces production, body weight, vital parameters, blood glucose and cortisol concentrations, abdominal palpation findings, facial grimace scale scores, and ethograms). RESULTS Mean surgery (43.2 vs 21.7 minutes) and anesthesia (76.2 vs 48.8 minutes) times were longer and mean incision length was shorter (24.0 vs 41.5 mm) for LapOVE versus OVE. No significant differences in postoperative variables were identified between groups. During LapOVE, small intestinal perforation occurred in 1 rabbit, which was then euthanized. Postoperative complications for the remaining rabbits included superficial incisional dehiscence (LapOVE, 1/5; OVE, 2/6), subcutaneous emphysema (LapOVE, 1/5; OVE, 0/6), and seroma formation (LapOVE, 1/5; OVE, 0/6). CONCLUSIONS AND CLINICAL RELEVANCE Surgery time for LapOVE was twice that of OVE, and LapOVE resulted in unique complications in rabbits. No evidence of a reduction in pain or faster return to baseline physiologic status was found for LapOVE. Further evaluation of LapOVE in rabbits is warranted, with modification to techniques used in this study or a larger sample size.