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EFFECT OF LOW LEVEL LEASER THERAPY ON THE CHRONIC DEFECT OF TIBIAL BONES IN RABBITS Полный текст
2018
Humam H. Nazht | Sinan A.N. Al-khazrajii | Raffal A. Omar
This project was designed to evaluate the effect of low level leaser therapy(LLLT) on the induced chronic defect in the tibial bone of rabbits radiographically ,and physically,eight adults local breed rabbits of both sexes were employed toinduced 2.5 mm in diameter circular defect in the tibial bone surgically under generalanesthesia and highly aseptic technique ,the induced hole was implanted with 2.5 mmstainless steel screw ,that removed from the bone after one and half month postoperation , the experimental animals randomly divided into two equal groups fouranimas of each, the control group was lifted for normal healing process of the bonedefect without any treatment ,while the treatment group was exposed daily with singledosage of continuous diode laser for five minutes of 850 nm and 148.4 j/cm2 at 72hours interval for 14 days post operation (p.o.) ,the radiographic results in thetreatment group showed increase in the bone density around the induced defect withnew bone formation fill the cavity at the end of 2nd week p.o. then increase in thebone density and volume at the end of 4th week p.o. compare with the control groupthat the density and the new bone formation less than treatment group ,the physicalanalysis revealed increase in the bone density and hardiness with significant increasein fractures tolerance in the treatment group compare with the control group .Inconclusion that the LLLT successfully used for repairing the critical ( chronic) defectin tibial bones in rabbits .
Показать больше [+] Меньше [-]ANEW METHOD FOR ESTIMATION MEBENDAZOLE IN ITS PHARMACEUTICAL PREPARATIONS AND IN CAMEL URINE Полный текст
2018
A simple, accurate and rapid spectrophotometric method has been developed for the estimation of mebendazole in pure, pharmaceutical formulations and camel urine. The method is based on the reaction of mebendazole with1-fluoro 2, 4-dinitrobenzene to produce orange-yellow colored complex having maximum absorption at 494 nm. Beeˈs law was obeyed over the concentration range of 20-280μg/ml, with molar absorptivity of 3.32x104 l/mol.cm. The present method is considered to be simple because it does not need heating, hydrolysis, and solvent extraction steps. The proposed method has been successfully applied for the determination of mebendazole in pure, pharmaceutical formulations and in camel urine.
Показать больше [+] Меньше [-]EFFECTS OF HEXANE OF RICINUS (SEED AND LEAVES )AND BLACK PEPPER HEXANE EXTRACTS ON THE SOME BIOLOGICAL CONTROL OF Chrysomya albiceps Полный текст
2018
This study was carried out to investigate the effects of Hexane extracts of Ricinus (Seed & leaves ) and Black Pepper on Chrysomya albiceps larvae . The result showed that the Ricinus leaves Hexane extract had 100% mortality in all stage Larvae at 0.1&0.5mg\ml and 96.666 mortality in larvae at 1.0mg\ml of the same extract (Table 2). Black pepper Hexane extracts Result showed that 1.0mg\ml had mean mortality 86.666 in larvae (Table 3). Ricinus seed Hexane extract showed 83.333 mean mortality rate at 1.0 mg\ml (Table 1 ).Many concentration were prepared (0.1,0.5,1.0 )mg\ml for each plants extracts in our investigation .Chemicals plants compounds were mage red (Alkaloids, Phenols, Tannins, Resins, and Oils ). The result showed that’s used of natural plants production had better than the chemicals insecticides which it studied in another time before this our investigation.
Показать больше [+] Меньше [-]HEMATOLOGICAL PROFILE OF RATS TREATED WITH QUERCETIN DERIVATIVE AGAINST CARBON TETRACHLORIDE (CCL4 ) TOXICITY Полный текст
2018
The effect of quercetin derivative (1-(-2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4 H –chromen-4-ylidene) thiourea coded (QTU) on the hematological profile of Wistar rats induced toxicity with carbon tetrachloride (CCl4) was investigated. Administration intraperitoneal injection of 12.5 mg /kg and 25mg/kg of quercetin derivative (QTU), after four weeks of being exposed to CCl4, led to a significant decrease (p<0.05) in WBC count, with a corresponding increase on PCV and Hb. It was also observed that the functional recovery of these blood indices is concentration dependent. Results of this study revealed a depletion of RBC and a decrease in Packed Cell Volume (PCV) and Hemoglobin (Hb), In addition, an elevation in the levels of White Blood Cell (WBC) caused by CCl4 compared to control samples.
Показать больше [+] Меньше [-]EFFECT OF IRAQI SWEET ALMOND (Prunus amygdalus) ON SOME PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS IN ROCK DOVE PIGEONS (Columba livia gaddi) TREATED WITH LEAD ACETATE Полный текст
2018
The current study aims to evaluate the effect of Iraqi sweet almond (Prunus amygdalus) on the toxicity of lead acetate in wild pigeons Rock Doves (Columba livia gaddi) on body weight and certain hematological and biochemical parameters. Forty healthy adults of both sexes Rock Dove pigeons were randomly selected and categorized into four groups, A,B,C, and D. Group A (control group), were fed on a balanced ration, group B, were administered lead acetate 72 mg/kg body weight orally, group C orally administered with an aqueous extract of Iraqi sweet almond (Prunus amygdalus) 300 mg/kg body weight, and group D were orally administered with 72 mg/kg body weight of lead acetate and 300 mg/kg body weight of the aqueous extract of Iraqi sweet almond (Prunus amygdalus). These materials were administered daily and for the whole study period which extended to 28 days. Results showed that lead acetate alone caused significant decrease (P≤ 0.05) in the body weight, hemoglobin concentration, total erythrocyte count, PCV% and triglycerides, while there were a significant increase (P≤ 0.05) in total leukocyte count, heterophils, monocytes, lymphocytes, glucose, uric acid, and AST and ALT activities. On the other hand, combined treatment with Iraqi sweet almond and lead acetate to members of group (D) caused significant increase (P≤ 0.05) in hemoglobin, total erythrocyte count, PCV% and lymphocytes, and significant decrease (P≤ 0.05) in cholesterol and uric acid, while no significant differences in triglyceride and AST and ALT activities. The present finding concludes that adding of Iraqi sweet almond has significant effects on certain hematological and biochemical properties of Rock Dove pigeons (Columba livia gaddi) affected by administering lead.
Показать больше [+] Меньше [-]EFFECT OF POLICOSANOL EXTRACT ADMINISTRATION ON LIPID PROFILE IN HYPERCHOLESTEROLEMIC FEMALE RATS DURING LACTATION Полный текст
2018
The present study designed to investigate the effects of the extracted policosanol on the lipid profile of hypercholesterolemic lactating female rats (Ratus norvigicus). Forty-eight female rat (10-12) weeks old, and weighting (200-250 gm) were used in the current study. After daily vaginal smear for 3 sequences estrus cycles, they mated with twenty-four healthy adult fertile males weighting (280-350 gm), 24 of these females were induced to be hypercholesterolemic during the pregnancy, and others still healthy. Sugarcane was collected from Mesan province and policosanol was extracted from sugarcane wax. Immediately after birth, the animals were divided into 6 groups: Group 1 (n=8) (Control): Normal rats were given only 0.5 ml/ animal of Dimethyl Sulphoxide (DMSO) daily immediately after birth. Group 2 (n=8) hyperchol. (HC): Hpercholestrolemic rats were given only DMSO 0.5 ml/ animal daily immediately after birth. Group 3(n=8) (HC+SM): Hpercholestrolemic rats were given simvastatin at dose 20mg/kg BW/ day dissolved in DMSO orally by gavages' immediately after birth. Group 4(n=8) (SM): Normal rats were given only simvastatin at dose 20mg/kg BW/ day dissolve xsqqs ed in DMSO orally by gavages' immediately after birth. Group 5 (n= 8) (HC+ST. pol): Hypercholesterolemic rats were given standard policosanol at dose 20mg/kg BW/day orally by gavages' immediately after birth. Finally, group 6(n=8)( HC+EX. pol): Hypercholesterolemic rats were given policosanol extraction at dose 20mg/kg BW/day orally by gavages immediately after birth. The treatment continued until weaning ( 30 days). All animals were then sacrificed and blood samples were collected for measuring of lipid parameters. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) in all groups. Results revealed a significant improvement in TC level was recorded in (HC+SM) group and no difference was observed as compared with the control group. While the level of TC in (SM), (HC+ST.pol) and (HC+EX.pol) treated groups decreased significantly (p ≤ 0.05) compared with the control group, (HC) and (HC+SM) treated groups. No significant differences in HDL-C were observed between (HC+EX.pol) and control group. While the level of HDL-C still significantly lower in all other treated groups compared with the control group. A significant reduction (p ≤ 0.05) in LDL-C level was recorded in all treated groups compared with (HC) group. No significant differences were observed in LDL-C between (SM), (HC+ST. pol) and (HC+EX.pol) compared with the control group. While the LDL-C still significantly higher (p ≤ 0.05) in (HC+SM) compared with the control group. Levels of triglyceride (TG) in (HC+SM),(HC+ST.pol) and (HC+EX.pol) treated groups recorded a significant elevation ( p≤ 0.05) as compared with the control group. While levels of TG in (SM) treated group was significantly decreased (p≤0.05) as compared with the control group. Conclusion: local sugar cane can be used to extract the policosanol which has an antihyperlipidemic effect on hypercholesterolemic lactating female rats.
Показать больше [+] Меньше [-]SYNERGISTIC EFFECTS OF ETHYLENE DIAMINE TETRA ACETIC ACID AND IMIPENEM AGAINST Pseudomonas aeruginosa WERE ISOLATED FROM CHEESE IN HILLA PROVINCE. Полный текст
2018
The study was performed to the study synergistic effect of Ethylene diamine tetra acetic acid (EDTA) and Imipenem against Pseudomonas aeruginosa were isolated from cheese at Hilla province. Cheese samples 70 were collected randomly from (Retail, supermarkets and dairy shops) in Hilla and were transported to the laboratory. The result revealed (45%) Pseudomonas aeruginous detecte were collected from cheese. These isolates were tested for disk diffusion method for susceptibility of imipenem. Eight isolates were resistant , and (84.3%) of Pseudomonas aeruginosa strain were sensitive (84.3%) to EDTA alone. Also, the result showed of Synergistic EDTA- IMP disc diffusion against (8) isolate of Pseudomonas aeruginosa revealed (87.5%) isolates were sensitive. However; the The result showed significant (P < 0.05) of susceptibility to (8) P. aeruginosa isolates to imipenem with and without EDTA. In conclusion, according to the present study can use oxidant agent inhibition of growth bacteria specific P. aeruginosa such as ethylene diamene tetra acidic acid (EDTA) and can used in preserving food specific sold cheese.
Показать больше [+] Меньше [-]RELATIONSHIP BETWEEN WEIGHT AND MORTALITY RATIO OF COMMON CARP CYPRINUSCARPIOCULTIVATED IN FLOATING CAGES DURING ACUTE INCREASING OF TEMPERATURE Полный текст
2018
Current study was conducted in 13 floating cages located in Shatt Al-Arab River bank at Al-Hartha District. Common carp Cyprinuscarpio of different weights (290-1700 gm) and numbers (400-1047) fish/cage was cultivated in these cages. Observation was also taken from earthen pond at the same location cultivated with common carp, silver carp Hypophthalmichthys molitrix and grass carp Ctenopharyngodonidella. Results showed that water temperature range between 30-35 0C during May-June, 2015 and a range of 36- 38 0C during July-August, 2015 in floating cages, while in earthen pond there were 1 0C more. Salinity don’t differ too much during experiment and range between 1.5-3 ppt. Results of earthen pond revealed no fish mortality during experiment for three species cultivated in this pond. Results appeared that fish mortality during May-June don’t largely differed and its ranged between 0.709%-1.861% and there weren’t any relationship between fish mortality and fish weight. Different fish mortality during July-August which ranged between 3.88% for average fish weight of 400 g and total fish mass of 412 kg and 14.75% for average fish weight of 1700 g and total fish mass of 680 kg. Significant correlation (r=0.86) was found between average fish weight and fish mortality.Significant correlation (r=0.72) was also found between total fish weight and fish mortality. From previous results we concluded that there were positive relationship between fish mortality and fish weight during July-August.
Показать больше [+] Меньше [-]Peste des petits ruminants virus infection of Black Bengal goats showed altered haematological and serum biochemical profiles Полный текст
2018
Shahana Begum | Mohammed Nooruzzaman | Murshida Parvin | Nijaya Mohanto | Rokshana Parvin | Mohammad R. Islam | Emdadul H. Chowdhury
Peste des petits ruminants virus infection of Black Bengal goats showed altered haematological and serum biochemical profiles Полный текст
2018
Shahana Begum | Mohammed Nooruzzaman | Murshida Parvin | Nijaya Mohanto | Rokshana Parvin | Mohammad R. Islam | Emdadul H. Chowdhury
In Bangladesh, veterinarians often claim to reduce the mortality of natural peste des petits ruminants (PPR) outbreaks with the help of supportive fluid and electrolyte therapy. Information on haematological and biochemical parameters of PPR-infected goats, which is often altered because of associated tissue damages, is necessary to formulate the appropriate supportive therapy. This study determined the haematological and serum biochemical parameters of Black Bengal goats naturally infected with PPR virus. Blood and serum samples from 13 PPR-affected Black Bengal goats from 13 field outbreaks and 5 healthy goats were collected and analysed by routine haematological and biochemical examination. Haematological analysis of PRR-affected goats showed severe anaemia characterised by significant decrease in the values of haemoglobin, total erythrocyte counts (TECs) and packed cell volume (PCV). On the contrary, PPR-affected goats showed marked leucocytosis with absolute increase in lymphocytes and neutrophils counts compared to the healthy goats. Biochemical analysis revealed significant decrease in total protein and albumin level and increased creatine kinase, aspartate transaminase and alanine transaminase that mirrored the gross and histopathological changes in the PPR-affected goats. Significant increase in the values of sodium and chloride ions was found in the sera of PPR-infected goats. Peste des petits ruminants virus altered the haematological and serum biochemical parameters of the infected goats. Antidiarrheal agents with aqua solution together with other drugs to support liver and kidney function could help improve therapy of PPR-infected goats.
Показать больше [+] Меньше [-]Peste des petits ruminants virus infection of Black Bengal goats showed altered haematological and serum biochemical profiles Полный текст
2018
Begum, Shahana(Bangladesh Agricultural University Department of Pathology) | Nooruzzaman, Mohammed(Bangladesh Agricultural University Department of Pathology) | Parvin, Murshida(Bangladesh Agricultural University Department of Pathology) | Mohanto, Nijaya(Bangladesh Agricultural University Department of Pathology) | Parvin, Rokshana(Bangladesh Agricultural University Department of Pathology) | Islam, Mohammad R.(Bangladesh Agricultural University Department of Pathology) | Chowdhury, Emdadul H.(Bangladesh Agricultural University Department of Pathology)
In Bangladesh, veterinarians often claim to reduce the mortality of natural peste des petits ruminants (PPR) outbreaks with the help of supportive fluid and electrolyte therapy. Information on haematological and biochemical parameters of PPR-infected goats, which is often altered because of associated tissue damages, is necessary to formulate the appropriate supportive therapy. This study determined the haematological and serum biochemical parameters of Black Bengal goats naturally infected with PPR virus. Blood and serum samples from 13 PPR-affected Black Bengal goats from 13 field outbreaks and 5 healthy goats were collected and analysed by routine haematological and biochemical examination. Haematological analysis of PRR-affected goats showed severe anaemia characterised by significant decrease in the values of haemoglobin, total erythrocyte counts (TECs) and packed cell volume (PCV). On the contrary, PPR-affected goats showed marked leucocytosis with absolute increase in lymphocytes and neutrophils counts compared to the healthy goats. Biochemical analysis revealed significant decrease in total protein and albumin level and increased creatine kinase, aspartate transaminase and alanine transaminase that mirrored the gross and histopathological changes in the PPR-affected goats. Significant increase in the values of sodium and chloride ions was found in the sera of PPR-infected goats. Peste des petits ruminants virus altered the haematological and serum biochemical parameters of the infected goats. Antidiarrheal agents with aqua solution together with other drugs to support liver and kidney function could help improve therapy of PPR-infected goats.
Показать больше [+] Меньше [-]Immunogenicity of a plasmid DNA vaccine encoding G1 epitope of bovine ephemeral fever virus G glycoprotein in mice Полный текст
2018
Reza Pasandideh | Masoud Reza Seyfi Abad Shapouri | Mohammad Taghi Beigi Nassiri
Immunogenicity of a plasmid DNA vaccine encoding G1 epitope of bovine ephemeral fever virus G glycoprotein in mice Полный текст
2018
Reza Pasandideh | Masoud Reza Seyfi Abad Shapouri | Mohammad Taghi Beigi Nassiri
The aim of this study was to investigate the immunogenicity of a plasmid deoxyribonucleic acid (DNA) vaccine encoding the G1 epitope of bovine ephemeral fever virus (BEFV) G glycoprotein in mice. A plasmid DNA carrying the G1 gene was constructed and designated as pcDNA3.1-G1. The expression of the target gene was confirmed in human embryonic kidney 293 (HEK 293) cells transfected with pcDNA3.1-G1 by indirect immunofluorescent staining. Immunisation experiments were intramuscularly carried out by vaccinating 6-week-old female mice in four groups, including the pcDNA3.1-G1 construct, pcDNA3.1 (+) plasmid alone, BEF-inactivated vaccine and phosphate-buffered saline (PBS) (1X) three times with 2-week intervals. Fourteen days after the last immunisation, the animals were bled and the resulting sera were tested for anti-G1-specific antibodies by immunoblotting analysis, indirect enzyme-linked immunosorbent assay (ELISA) and virus neutralisation (VN) test. Serological assays showed that the pcDNA3.1-G1 construct expressing G1 protein was able to elicit specific antibodies against this antigen. Virus neutralisation test showed that pcDNA3.1-G1 could induce anti-BEFV-neutralising antibodies in mice. Our findings indicated that a new dimension can be added to vaccine studies for bovine ephemeral fever (BEF) using eukaryotic expression plasmids encoding the G1 antigen in the future.
Показать больше [+] Меньше [-]Immunogenicity of a plasmid DNA vaccine encoding G1 epitope of bovine ephemeral fever virus G glycoprotein in mice Полный текст
2018
Pasandideh, Reza(Natural Resources University Department of Animal Science, Khuzestan Agricultural Sciences) | Shapouri, Masoud Reza Seyfi Abad(Shahid Chamran University of Ahvaz Department of Pathobiology) | Nassiri, Mohammad Taghi Beigi(Natural Resources University Department of Animal Science, Khuzestan Agricultural Sciences)
The aim of this study was to investigate the immunogenicity of a plasmid deoxyribonucleic acid (DNA) vaccine encoding the G1 epitope of bovine ephemeral fever virus (BEFV) G glycoprotein in mice. A plasmid DNA carrying the G1 gene was constructed and designated as pcDNA3.1-G1. The expression of the target gene was confirmed in human embryonic kidney 293 (HEK 293) cells transfected with pcDNA3.1-G1 by indirect immunofluorescent staining. Immunisation experiments were intramuscularly carried out by vaccinating 6-week-old female mice in four groups, including the pcDNA3.1-G1 construct, pcDNA3.1 (+) plasmid alone, BEF-inactivated vaccine and phosphate-buffered saline (PBS) (1X) three times with 2-week intervals. Fourteen days after the last immunisation, the animals were bled and the resulting sera were tested for anti-G1-specific antibodies by immunoblotting analysis, indirect enzyme-linked immunosorbent assay (ELISA) and virus neutralisation (VN) test. Serological assays showed that the pcDNA3.1-G1 construct expressing G1 protein was able to elicit specific antibodies against this antigen. Virus neutralisation test showed that pcDNA3.1-G1 could induce anti-BEFV-neutralising antibodies in mice. Our findings indicated that a new dimension can be added to vaccine studies for bovine ephemeral fever (BEF) using eukaryotic expression plasmids encoding the G1 antigen in the future.
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