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Endotoxin-induced production of interleukin 6 by equine peritoneal macrophages in vitro.
1992
Morris D.D. | Crowe N. | Moore J.N. | Moldawer L.L.
A study was performed to determine whether equine peritoneal macrophages produce interleukin 6 (IL-6) in vitro in response to endotoxin. Peritoneal fluid was collected from 14 clinically normal adult horses and was used as the source of peritoneal macrophages. Macrophages from each horse were isolated and cultured separately in vitro in the absence or presence of various concentrations (0.5, 5, or 500 ng/ml) of endotoxin (lipopolysaccharide from Escherichia coli 055:B5). Culture medium supernatants were collected after 3, 6, 12, and 24 hours' incubation and were frozen at - 70 C until assayed for IL-6 activity. Supernatant IL-6 activity was determined by use of a modified colorimetric assay and the murine hybridoma cell line B13.29 clone B.9, which is dependent on IL-6 for survival. Results indicated that equine peritoneal macrophages produce IL-6 in vitro and that supernatant medium IL-6 activity was significantly (P < 0.05) increased by exposure to endotoxin. Significant (P < 0.05) time and treatment effects on macrophage IL-6 production were apparent. The IL-6 activity peaked at 6 or 12 hours' incubation, then remained high through 24 hours' incubation, regardless of endotoxin exposure. Medium IL-6 activity during 3 and 6 hours' incubation was significantly (P < 0.05) greater in macrophages exposed to 5 or 500 ng of endotoxin/ml than in those exposed to 0.5 ng of endotoxin/ml; however peak IL-6 activity was similar among all endotoxin concentrations. Endotoxin concentration did not have an effect on medium IL-6 activity from macrophages exposed to endotoxin for 12 or 24 hours.
Показать больше [+] Меньше [-]Parthenogenetic development of mouse eggs-(1)-Parthenogenetic activation by ethanol and hyaluronidase treatments.
1992
Lee H.J. | Kang T.Y. | Choi M.C. | Ha D.S.
Laboratory cultivation of blue-green algae for use as a food for Lymnaeids, the intermediate host of Fasciola hepatica.
1992
Lee C.G. | Kim S.K. | Lee C.Y.
Reverse effects of electroacupuncture on xylazine analgesia in goats.
1992
Seo K.M. | Yoon J.H. | Cheong J.T. | Chang K.H. | Nam T.C.
Isolation and morphological characterization of natural killer cell in the Sprague-dawley (SD) rats.
1992
Kang K.S. | Lee Y.S.
Comparison of soluble antigens Leptospira interrogans serovars by SDS-PAGE, crossed immunoelectrophoresis and immunoblotting.
1992
Baik Y.O. | Mah J.S.
Effect of Artemisia Iwayomogi water extract on hepatic injury by carbon tetrachloride in rats - (1) - Effect of serum AST, ALT, LDH activities, lipid content and liver peroxide content.
1992
Kim K.S. | Park J.H.
Spontaneous lingual papillomas in fischer 344 rats.
1992
Kang B.H. | Lim C.H.
Ultrastructure of virus particles in the liver of piglets infected with porcine enterovirus serotype 3.
1992
Shin T.K | Lee C.S. | Huh M.D.
Effect of bilateral stimulation on spinal evoked potentials in dogs Полный текст
1992
Poncelet, Luc | Delauche, A. | Vinals, C. | Balligand, Marc
peer reviewed
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