Objective: This study aimed at assessing the prevalence of Helicobacter spp. in ice cream sold at Qena city (Egypt) with special reference to H. pylori, and to study the antimicrobial effect of mastic gum essential oil on H. pylori. Materials and methods: A total of 60 small and large scale ice cream samples (30 each) were investigated for the presence of Helicobacter spp. Then, the essential oil of mastic gum was added to lab prepared ice cream inoculated with H. pylori isolate in a count of Log6 using 2 concentrations of 0.8 and 1.6% and one group was kept as a control group. Each group was subdivided into 2 sub-groups; one was kept at -5°C and another one was kept at -20°C. Results: Helicobacter spp. could be isolated from 11.7%, while, H. pylori could be found in 3.3% of the total examined ice cream samples. Regarding the anti-Helicobacter pylori effect of mastic gum essential oil added to lab prepared ice cream, in samples kept at -5°C it was found that after hardening the count fall into Log5 in both control and 0.8% groups and log4 in 1.6% essential mastic oil concentration. Reduction in H. pylori at the first week and third day of storage reached 100% where H. pylori couldn’t be counted or isolated from both 0.8 and 1.6% concentrations, respectively. While in ice cream samples stored at -20°C, the reduction in H. pylori load was rapid at first, it was log4 and log3 in groups of 0.8% and 1.6% concentrations, respectively versus log5 in control samples. At the third day H. pylori was not able to be counted or isolated at third day of storage for both concentrations. Conclusion: All isolates recovered from small scale ice cream samples reflexing the hygienic conditions under which samples were produced. Mastic gum essential oil exhibited a powerful anti-H. pylori effect recommending its addition to food matrix for therapeutic purposes or as a functional food. [J Adv Vet Anim Res 2017; 4(2.000): 132-139]

Objective: This study was conceived to investigate the pathogenicity of relapsed (Diminazene aceturate-resistant), field (original) and mixed (relapsed and field) isolates of Trypanosoma brucei brucei in rats. Materials and methods: Twenty eight healthy adult albino rats of both sexes weighing between 149-177 gm were used to compare the pathogenicity of relapsed, field and the mixed isolates of T. brucei brucei infections. The rats were separated into four groups (A-D); where, group A was kept as uninfected control, and group B was infected with 1x103 trypanosomes of the field isolate and 1x103 trypanosomes of the diminazene aceturate resistant isolate. The rats of groups C and D were infected with 1x106 trypanosomes of the diminazene aceturate-resistant isolate and 1x106 trypanosomes of the field isolate, respectively. Results: The infected rats became parasitemic within 4 to 8 days post-infection. The mean pre-patent periods (PP) were 4.1&plusmn;1.1, 6.0&plusmn;2.0 and 9.1&plusmn;1.1 days in groups B, C and D respectively, while the mean survival time (ST) in groups B, C and D were 21.4&plusmn;10.1, 27.1&plusmn;13.2 and 34.0 &plusmn;12.8 days, respectively. The PP and ST were shortest (P<0.05) in group B (mixed infections), and level of parasitemia was higher (P<0.05) in group B (mixed infections) as compared to groups C and D. The level of anemia was comparable (P>0.05) in groups C and D and more severe (P<0.05) in group B. Conclusion: Mixed infections exhibit shortest PP, ST, higher level of parasitemia and more severe anemia, and appear to be more pathogenic. [J Adv Vet Anim Res 2017; 4(1.000): 97-103]

Objective: The study was conducted to determine the prevalence of infectious diseases in Sonali chickens at Bogra Sadar Upazila, Bogra, Bangladesh. Materials and methods: A total of 258 sick and dead Sonali chickens were examined for the diagnosis of different infectious diseases based on history, clinical findings and postmortem lesions of dead and sacrificed birds. Results: Infectious Bursal disease (IBD) was recorded in 14.72% (n=38/258) cases. Similarly, Newcastle disease (ND), Coccidiosis, Colibacillosis and Mycoplasmosis were recorded in 11.24% (n=29/258), 13.95% (n=36/258), 14.72% (n=38/258), 12.79% (n=33/258) cases, respectively. Mixed infection of IBD, ND and Coccidiosis found in 16.67% (n=43/258) birds. On the other hand, mixed infection of IBD, ND and colibacillosis was recorded in 15.89% (n=41/258) cases. Conclusion: It is concluded that several infectious diseases are commonly present in Sonali chicken in the study area of Bangladesh. Mixed infections are more prevalent as compared to single infection. Proper hygienic management and appropriate vaccination should be taken in consideration for effective control the diseases. Further microbiological and molecular diagnoses are suggested for detail studies of these diseases and their pathogens. [J Adv Vet Anim Res 2017; 4(1.000): 39-44]

Objective: Postoperative complication of extramural ectopic ureters (EEUs) with persistent urinary incontinence (UI) is common in Siberian Huskies. This case report was aimed at reporting the successful correction procedure of EEU in Siberian Huskies by surgical procedure. Materials and methods: A three-month-old and weighing 7.9 kg Siberian Husky dog was presented with history of an acute dermatitis around the genitals, swelling of the genitals and dribbling urination. Abdominal radiographs, ultrasonography, and 3D computed tomography scanning revealed presence of the ectopic ureter with urinary incontinence. Ureteroneocystostomy was applied as the first choice of corrective procedure in this case, and clinical sings were corrected apparently after surgery. Unfortunately, UI with cystitis was developed two months postoperatively. To correct the condition, surgical procedure of colposuspension with ovarianhystectomy and cystopexy was opted. Results: Post-operative progression showed good prognosis and the dog recovered fully. Follow up checkup of the patient after 4 months postoperative and follow up phone call 27 months later did not reveal any abnormalities. Conclusion: This case report recommends surgical procedure of modified colposuspension following ureteroneocystostomy to correct EEUs and its postoperative complication. [J Adv Vet Anim Res 2017; 4(3.000): 301-306]

Objective: This experiment was conducted to evaluate the therapeutic potency of marigold flower (Calendula officinalis) and turmeric (Curcuma longa) rhizome paste in wound healing. Materials and methods: Thirty six aseptic surgical wounds were tooled in six non-pregnant black Bengal goats dividing them in 3 groups. Month long information and follow-up examinations along with complications such as edema, wound dehiscence, suture abscess, exudation etc. were studied. Wound healing was assessed by observing some morphological characters as well as histopathological changes of the wounded area. Results: Results revealed that negligible elevation of suture line (1.17±0.11 mm) and significant (P [J Adv Vet Anim Res 2017; 4(4.000): 333-342]

Objective: Cattle are regarded as the principle reservoir of O157 and non-O157 shigatoxigenic Escherichia coli (STEC). Spreading of the STEC to human is primarily happens through contaminated meat, milk and their byproducts. The present study was aimed to explore the occurrence of STEC in the rectal swab of apparently healthy cattle. Materials and methods: A total of 60 E. coli isolates that were previously isolated from the rectal swab of cattle were used in this study. DNA were extracted from the isolates and screened by PCR to detect E. coli stx (stx1, stx2), ehxA and rfbO157 genes. Representative amplicons of the PCR products were sequenced. The prevalence of the STEC was determined based on the detection of STEC specific stx genes. The prevalence data were further analyzed by SPSS to elucidate any difference among different demographic groups of the study population. Results: Overall, 43.33% (n=26/60) of the isolates were found carrying stx genes. Based on the presence of stx and ehxA genes, 6 different types of STEC were identified, of which 20% (n=12/26) were carrying both stx1 and stx2 genes. None of the isolates was positive for rfbO157. The PCR amplicons were sequenced, and the nucleotide sequences were deposited in GenBank (accession: KM596779-KM596784). Conclusion: In this study, non-O157 STEC were found highly prevalent in the local cattle. This study suggests that the apparently healthy cattle may act as a potential source of STEC infection for humans. [J Adv Vet Anim Res 2017; 4(2.000): 194-199]

This research was conducted in the District Mangko Tano East Luwu in South Sulawesi Province, in 2017. The area of research is supported by oil palm plantations, palm plantations, agricultural land, forestry land, vacant land that has not been explored by farmers and other vacant land. The study was conducted by the method of field survey, according to information from the local Animal Husbandry Department, the criteria in the data is the cattle population in farmer.The purpose of this study was to determine the economic analysis on a group of cattle ranchers. Primary data and secondary data were analyzed using descriptive tabulation, quantitatively and economically. The results of the research effort shows that beef cattle, ranchers group profit amounted Rp.3.637.500/year, Agung Sindo  Village B/C ratio of 1.2, Kalaenaliri village of Rp.3.886.000/ year ratio B/C 1.2, and the Wanasari Village of Rp.3.788.000/year and ratio of B/C 1.2. Forage in locations very research support to the development of beef cattle Bali. But farmers are accustomed way of raising beef cattle between 2-4 head/breeder, so it needs additional maintenance business scale between 5-10 cattle/livestock farmers, so that the business more effectively and efficiently and economically will benefit farmers.

This study was carried out to assess the prevalence of gastrointestinal nematode in cattle in the Pintu Rime Gayo, a highland sub district of Bener Meriah Regency, Aceh according to age and sex. Stool samples were collected from 150 cattle aged 0-6, 7-12 and 12 months old. Examination was performed by using a centrifuge. Samples tested positive when found gastrointestinal nematode parasite eggs. The data obtained were analyzed with Chi-Square. The results showed that the prevalence of gastrointestinal nematode in cattle aged 0-7 months (32%) was significantly higher (p0.05) than that in cattle aged 7-12 months (22%) and 12 months (12%). The parasite was markedly more prevalent (p0.05) in female cattle (23.9%) than that in male cattle (15.2%). In conclusion, susceptibility of cattle in the Pintu Rime Gayo highland to gastrointestinal nematode infection was influenced by both age and sex.

Abstract

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