BACKGROUND: The induction of triploidy is an effective strategy for the production of sterile fish for aquaculture and has usually been reported to be accompanied by modifications in physiological characteristics. OBJECTIVES: The aim of this study was to assess the effects of triploidy induction on growth performance and amino acid profiles in rainbow trout. METHODS: Eight female (total weight 1600 ± 246 g) and six male (total weight 1393 ± 186 g) four-year-old healthy rainbow trout were used in this study. Triploidy was inducted through the application of heat shock of 28 ºC for 10 min to eggs 10 min post fertilization in an aquarium equipped with a heater. During the 38 days of rearing period, the fish were fed a rainbow trout commercial diet (BioMar, France) 12 times a day at the rate of 7% of their body weight. Fish ploidy level was determined by erythrocyte size measurement. RESULTS: Based on red blood cell analysis, the overall triploidization success level was 87.1%. Growth performance was significantly higher in diploids as compared to triploids (p<0.05). The levels of non essential amino acids increased and the levels of essential amino acids decreased as an effect of triploidy induction. CONCLUSIONS: Triploidy induction in rainbow trout affects growth performance and amino acid profiles in rainbow trout.

BACKGROUND: In aquaculture the fast growth, high feed efficiency and increased resistance against pathogens are favorable. One of the suitable approaches for improving the growth indices and resistance against pathogens is the application of probiotic bacteria. OBJECTIVES: The present study was conducted to evaluate the effect of nano/microencapsulated Lactobasillus bulgaricus by nano alginate/chitosan on the growth rate and feeding efficiency of great sturgeon (Huso huso) juveniles. METHODS: This experiment was conducted in a completely random design in four groups and group (5) with triplicate. 375 fish weighing (27.28±2.86 gr mean±SD), were randomly divided in to five equal groups in triplicates. The test groups were as follows: fish in T1 were fed with alginate/chitosan without bacterium, fish in T2 were fed with encapsulated bacteria with emulsification method, fish in T3 were fed with encapsulated bacteria with Ionicgelation method, fish in T4 were fed with only bacteria and control group. The fish were fed  3% of body weight per day. RESULTS: The results showed  the highest feed conversion ratio (FCR) was measured in T3 (1.64±0.11) and the lowest was observed in T4 (1.14±0.06).The highest values of SGR, PER, FER in T4 were seen among  the  treatments at days 30 and 60.                                                                                                                                             CONCLUSIONS: According the result, nanoalginat/Chithosan used for encapsulation of probiotics can be lead to decrease in growth performance compared to the other treatments.

BACKGROUND: Ambient temperature has a significant effect on the thyroid gland function. Thyroid gland can be assessed by ultrasonography and measurement of thyroid hormones. OBJECTIVES: The aim of this survey was normal ultrasonographic findings of thyroid gland in cat in warm (July) and cold (February) seasons. METHODS: The study was conducted on 30 native, adult healthy cats. Thyroid hormones including T3 and T4 were measured in each cat before ultrasonography. After finding thyroid gland, echogenesity, length, width and height of each lobe (right and left) were measured. RESULTS: Both thyroid lobes were scanable and symmetrical on each side. In sagittal plane it had a fusiform shape and in transverse plane it appeared as an oval or roughly triangular structure. The thyroid parenchyma was homogenously echogenic. The echogenecity of the gland was more than the neck muscles and no difference was seen between cold and warm seasons. Dimensions of each lobe were slightly larger in cold season than warm season. The differences in width and volume were significant (p<0.05) but the differences were not significant in length and height (p>0.05). The mean of the length, width, height and volume of both lobes were in normal range for cat with the same weight. Total means of length, width, height (mm) and volume (cm3) of both lobes were 17.2, 2.6, 2.7, and 0.07 in two seasons respectively. Also, the difference was significant in concentration of T3 and T4 hormones between cold and warm seasons, so that thyroid hormone concentrations were higher in cold season than warm season (p<0.05). CONCLUSIONS: It seems that the reduction in air temperature has an extraordinary effect on increasing thyroid gland size and thyroid hormone concentrations in native adult cats in Ahvaz district. This effect can be due to the influence of cold in the increase of body metabolism and thyroid gland function.

BACKGROUND: Rhipicephalus (Boophilus) annulatus is an important tick, which can transmit parasites and bacteria to cattle. It can also cause hides damage, growth reduction and sometimes paralysis. So, the tick infestation can be regarded as a major problem in the livestock industry. Different control methods including the chemical ones are used to fight ticks, but because of developing resistance to chemical treatments, researchers try to find some immunogenic proteins for vaccine production. Investigating these tick proteins could be an important step in the identification of biological molecules for the purpose of developing control strategies. OBJECTIVES: The aims of present study were to evaluate and analyze Boophilus annulatus larval extract proteins by 2- dimensional gel electrophoresis patterns (proteomic profiling) and identification of some its immunogenic proteins by two dimensional immunoblotting and  MALDI TOF/TOF mass spectrometry  referred to as Mazandaran strains. METHODS: The steps followed here were: tick preparation and culture, 1-D electrophoresis, and then 2-D electrophoresis, Western blotting and Mass spectrometry and then Mass data were analyzed by Mascot software. RESULTS: Analysis of the produced 2D image identified approximately 80 protein spots with different Molecular weight and PI by Coomassi blue staining. Based on immunogenicity (through Western blotting) and high concentration, 10 protein spots (between 14 and 97 kDa) candidates for MALDI TOF and MALDI TOF - TOF MS.  Among the 10 proteins spots, Vitellogenin, Vitellogenin-2 precursor, tropomyosin, hypotethical protein ISCW001652 were identified proteins with immunogenic properties. Also, quantification analysis showed some proteins had more quantity in soluble larvae protein extract and some such as vitellogenin had some isoforms. CONCLUSIONS: The results of this study could be a preliminary step towards selecting proteins candidated to develop vaccines against ticks.

BACKGROUND: Aflatoxin contamination in animal and poultry and its carry over to human beings is important in causing different diseases like Hepatitis and Liver Cirrhosis and finding methods to lessen toxin adsorption in animal and poultry tissues has a direct impact on health of animal products. OBJECTIVES: Evaluating the impact of natural toxin binder in broilers contaminated with aflatoxin B1 on performance, immunity and morpholgy of intestine. METHODS: 400 day-old broiler chicks under 4 treatments, 5 replicates and 20 chicks per replicate in completely randomized design manner were studied for 42 days. Experimental treatments were: 1- negative control (basal diet with out aflatoxin contamination); 2- positive control (basal diet + 0.6 mg/kg aflatoxin B1; 3- basal diet along with 1g/kg natural toxin binder and 4- basal diet + 1g/kg natural toxin binder. RESULTS: Presence of aflatoxin in diet reduced all performance indexes significantly (p

BACKGROUND: Medicinal herbs are used in poultry production to improve carcass quality, immunity and also lessen the use of antibiotics. OBJECTIVES: The aim of this study was to investigate the effect of adding coriander seed and ginger root powders to diet on performance, carcass traits, intestinal E.coli count and some serum biochemical parameters of broiler chickens. METHODS: One-hundred-sixty Cobb500 chicks were divided into four treatment groups with four replicates. Treatments as a completely randomized design were 1) basal diet (control), 2) basal diet plus 1.5% of coriander seed, 3) basal diet plus 0.75 %of ginger root powder, and 4) basal diet plus 1% coriander seed+ 0.5% of ginger root powder. At the end of the study, one bird from each replicate was slaughtered for blood sampling and carcass analysis. RESULTS: The results showed that the effect of treatments on feed conversion ratio of chickens was significant at during days 1-28 (p<0.05). The lowest feed conversion ratio was observed in coriander+ginger. Carcass analysis showed that the effect of treatments on abdominal fat weight, bourse and gizzard weights of chickens were significant (p<0.05). The greatest gizzard relative weight was in ginger+coriander group while the lowest abdominal fat and bourse relative weights were in ginger group. The treatments had significant effect on blood cholesterol and HDL concentrations (p<0.05). The lowest cholesterol level was observed in ginger group but the greatest HDL level was in coriander+ginger group. Effect of treatments on intestinal E.coli count was significant (p<0.05). Coriander+ginger treatment had the lowest E.coli in the ileum. CONCLUSIONS: The results showed that the level 0.75 ginger powder in broiler diets has positive effects on reduction of abdominal fat, bourse, cholesterol and also reduces the intestinal E.coli population. Ginger+coriander in the diet by reducing feed conversion ratio and population of E.coli caused an improvement in growth performance of broiler chickens.

BACKGROUND: In recent years, disorder in lipid metabolism has been increased markedly in companion animal’s population. Hyperlipidemia is a common sign of obesity in dogs, which is characterized by hypercholesterolemia or hypertriglycemia. OBJECTIVES:  The purpose of the present survey is to determine the effect of obesity on blood lipid profile changes (including triglyceride, total cholesterol, HDL-C and LDL-C) and comparing the decisive indicators of obesity in dogs in Ahvaz district. METHODS: Three groups of thirty dogs  referred to Veterinary Hospital of Ahvaz were studied between  2012-2014. The dogs in group A (control), had good body condition score (BCS= 4 to 5). The dogs were all thin (BCS= 1 to 3) in group B, and all obese in group C (BCS= 6 to 9). All of the studied dogs were selected from small breeds (Terrier, Spitz, Pekingese and Shih Tzu), of both sexes and ranging from 1 to 7 years old. Fasting blood samples were collected from all dogs and lipid profiles including serum triglycerides, total cholesterol, HDL and LDL were measured using commercial kits. RESULTS: Hyperlipidemia was confirmed in dogs of group A (normal) in 4 cases (13.33%), group B (thin) one case (3.33%), and group C (obese) in 19 cases (63.33%). Data analysis showed that there was a significant difference between group C (224.83±74.34) with groups A (149±39.66) and B (131.80±24.37) for triglyceride level (p<0.001), as well  a significant difference was seen between group C (229.40±60.36) with groups A (178.80±50.17) and B (151.47±23.45) and between group A with B for cholesterol level (p<0.05). The difference was significant for LDL-C between group C (67.10±22.83) with groups A (47.97±13.01) and B (43.07±13.08) (p<0.001), but there was no difference between various groups for HDL-C (p>0.05). The effects of age, gender and breed on the measured values did not show a significant difference between three groups (p>0.05). CONCLUSIONS: The present survey showed that there was a correlation between obesity and hyperlipidemia in dogs. Determination of body condition score (BCS) can be as a predictive agent in characterization of lipid profile status. Modification of diet and weight loss is necessary in obese dogs.

BACKGROUND: Many studies have demonstrated the important role of hippocampal serotonergic receptors in the emotional memory formation. Therefore, it is necessary to investigate the possible role of hippocampal serotonergic receptors in emotional memory formation in several animal model tasks. OBJECTIVES: The aim of this study was to investigate the possible role of ventral hippocampal (CA3) 5-HT4 receptors in emotional memory formation of rats in elevated plus-maze task. METHODS: 102 male Wistar rats were divided to 16 groups  (n=7), randomly. Two guide cannulae were implanted bilaterally, into the left and right hippocampi, using stereotaxic apparatus. At the test time and following the injections of saline, 5-HT4 receptor’s agonist and/or antagonist, the elevated plus-maze was used for evaluation of the emotional memory. RESULTS: A pre-test intra-CA3 injection of competitive agonist (RS67333; 1.2 µg/rat) and competitive antagonist (RS23597-190; 1.2 µg/rat) of 5-HT4 receptor, increased OAT% and OAE% by themselves while only the RS23597-190 decreased the closed arm entries, indicating that these drugs impaired the memory formation. Also, a pre-retest intra-CA3 injection of RS67333 and RS23597-190 (1.2 µg/rat) increased OAT% and OAE% by themselves, indicating that these drugs prevented the recall of the memories that formed on first day. CONCLUSIONS: The results revealed that the hippocampal 5-HT4 serotonergic receptor have an important role in memory formation and memory recall, at the time of emotional arousal and stressful situations.

BACKGROUND: Neospora caninum is a protozoanintracellular parasite which is considered as one of the main factors forrecurrent abortions of dairy cattle in various countries such as Iran. Thisparasite leads to negative economic impacts such as decline in reproduction,reduced amount of milk, and long calving intervals. OBJECTIVES: Therehave been numerous tests to determine the cause of abortion. PCR test isconsidered as a suitable method to specify Neospora caninum DNA and itcan determine the DNA in tissue samples and body fluids of the aborted fetus.This study aims to use PCR to evaluate parasites in the tissues of abortedfetuses so as to detect the best tissue for determining the parasite. METHODS:In this study, 82 aborted fetuses in the first six months of 2015 were studied.The tissues were selected from brain, liver, lungs, spleen, kidneys and rennetfluids. The NP21plus primer was used to detect the presence of Neosporacaninum in samples. After conducting the PCR Test, samples with 340bp bandin Gel electrophoresis were considered as positive. Statistical data from thesurvey of Neospora caninum’s presence in selected tissues were evaluatedby SAS (version 9.2) software. RESULTS: Contamination with this parasitewas found in 34 brain samples (41.5%) of aborted fetuses. In 2 (2.4%) and 4(4.9%) of the aborted fetuses, parasite DNA was found in lung and liver tissuesalong with brain tissues, respectively. CONCLUSIONS: Due to significantdifference of infection of brain tissues in comparison to other tissues, ourstudy considers brain tissue as the most appropriate sample for detecting Neosporacaninum infection in aborted fetuses in PCR method

BACKGROUND: In recent years, the use of biofloc has been considerable as a culture method to protect water quality and reduce the cost of food in the environment cultured shrimp. OBJECTIVES: The purpose of this study was to evaluate the effects of different levels of biofloc on water quality, growth performance and survival of Litopenaeus vannamei post larvae reared with no water exchange. METHODS: Post larvae with weight of 7.48 ± 0.85 mg and length of 9.23 ± 0.49 mm were reared in fifteen tanks with volume of 40-L and stocking density of 10 PL/L. Different levels of biofloc were added to tanks’ water as food sources: B0 (100% concentrate); B75 (75% biofloc +25% concentrate); B50 (50% biofloc +50% concentrate); B25 (25% biofloc +75% concentrate) and B100 (100% biofloc). The experiment was carried out for twenty=eight days. Results: Total ammonia nitrogen (TAN) level was lower in B25 than other treatments (P<0.05). The levels of Nitrate (mg NO3–N L−1) were significantly lower in B25 and B50 in 28 days of the experiment than in other treatments (P<0.05). At the end of the experiment, results showed that shrimp growth and survival were higher in B25 (301.23± 32.20 g and 81.56%, respectively) than in other treatments (P<0.05). The concentration of TSS tended to increase in the treatments during the experiment. CONCLUSIONS: The results from this study suggest that replacement of 25% biofloc (B25) with food concentrate can improve growth performance and water quality of the cultured shrimp.