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Associations of alleles of microsatellite markers with agronomical traits of modern bread winter wheat varieties in Southern Ukraine Полный текст
2016
Колесник, О. О | Хохлов, О. М | Чеботар, С. В
Associations of alleles of microsatellite markers with agronomical traits of modern bread winter wheat varieties in Southern Ukraine Полный текст
2016
Колесник, О. О | Хохлов, О. М | Чеботар, С. В
Purpose. Defining marker-trait associations of microsatellite markers with specific regions of the genome that control important agronomical traits in the investigated varieties originated in the Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigations and entered into the State register of plant varieties suitable for dissemination in Ukraine during different years. Methods. Molecular genetic methods (extraction of genomic DNA, polymerase chain reactions (PCR), electrophoresis of amplification products in polyacrylamide gel), field methods (phenological observations of heading date and analysis of plant height, visual assessment of the colour and length of wheat ear and awns), statistical methods (evaluation of trait means by descriptive statistic instruments of EXCEL package, ANOVA method performed by GLM instrument from AGROBASE 21 package). Results. During four growing years (2010/11, 2011/12, 2012/13, 2013/14), 47 bread winter wheat varieties were phenotypically measured and analyzed with 17 microsatellite loci. 35 marker-trait associations (MTA) for heading date, 39 for plant height, 33 for awn size, 20 for ear colour and 8 for ear size were found to be stable and significant during two–four different growing years. Conclusions. Microsatellite markers that showed substantial and stable during different growing years associations with agronomical traits can be useful and suitable for marker-assisted selection (MAS) in Ukrainian wheat breeding programs.
Показать больше [+] Меньше [-]Associations of alleles of microsatellite markers with agronomical traits of modern bread winter wheat varieties in Southern Ukraine Полный текст
2016
О. О. Колесник | О. М. Хохлов | С. В. Чеботар
Purpose. Defining marker-trait associations of microsatellite markers with specific regions of the genome that control important agronomical traits in the investigated varieties originated in the Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigations and entered into the State register of plant varieties suitable for dissemination in Ukraine during different years. Methods. Molecular genetic methods (extraction of genomic DNA, polymerase chain reactions (PCR), electrophoresis of amplification products in polyacrylamide gel), field methods (phenological observations of heading date and analysis of plant height, visual assessment of the colour and length of wheat ear and awns), statistical methods (evaluation of trait means by descriptive statistic instruments of EXCEL package, ANOVA method performed by GLM instrument from AGROBASE 21 package). Results. During four growing years (2010/11, 2011/12, 2012/13, 2013/14), 47 bread winter wheat varieties were phenotypically measured and analyzed with 17 microsatellite loci. 35 marker-trait associations (MTA) for heading date, 39 for plant height, 33 for awn size, 20 for ear colour and 8 for ear size were found to be stable and significant during two–four different growing years. Conclusions. Microsatellite markers that showed substantial and stable during different growing years associations with agronomical traits can be useful and suitable for marker-assisted selection (MAS) in Ukrainian wheat breeding programs.
Показать больше [+] Меньше [-]Physiological role of amino acids in the nutrition of highly productive varieties of winter wheat Полный текст
2016
Швартау, В. В | Михальська, Л. М | Мірошниченко, І. М
Physiological role of amino acids in the nutrition of highly productive varieties of winter wheat Полный текст
2016
Швартау, В. В | Михальська, Л. М | Мірошниченко, І. М
Purpose. To define the role of fertilizers containing amino acids in the nutrition systems of highly productive varieties of winter wheat. Methods. Field studies, biochemical technique, analytical procedure, statistical evaluation. Results. In the process of investigations, sufficiently high activity of fertilizers containing amino acids was established when applying them for leaf-feeding. Fertilizers based on algae (Megafol, Megafol Protein, Terra-Sorb foliar) and animal (Izabion) hydrolysates appeared to be very effective for increasing yield of culture. It was found that in case of low doses of mineral nitrogen, fertilizers helped to increase both the yield and quality indicators of high-yielding wheat variety. Conclusions. It was defined that fertilizers that include amino acids are highly effective compositions containing plant-available nitrogen in organic form and can be promising for application as antistress agents and improving efficiency of macro- and microelements use by cultivated plants. They are essential components of modern technologies for cultivation of highly productive varieties and hybrids in crop production of Ukraine.
Показать больше [+] Меньше [-]Physiological role of amino acids in the nutrition of highly productive varieties of winter wheat Полный текст
2016
В. В. Швартау | Л. М. Михальська | І. М. Мірошниченко
Purpose. To define the role of fertilizers containing amino acids in the nutrition systems of highly productive varieties of winter wheat. Methods. Field studies, biochemical technique, analytical procedure, statistical evaluation. Results. In the process of investigations, sufficiently high activity of fertilizers containing amino acids was established when applying them for leaf-feeding. Fertilizers based on algae (Megafol, Megafol Protein, Terra-Sorb foliar) and animal (Izabion) hydrolysates appeared to be very effective for increasing yield of culture. It was found that in case of low doses of mineral nitrogen, fertilizers helped to increase both the yield and quality indicators of high-yielding wheat variety. Conclusions. It was defined that fertilizers that include amino acids are highly effective compositions containing plant-available nitrogen in organic form and can be promising for application as antistress agents and improving efficiency of macro- and microelements use by cultivated plants. They are essential components of modern technologies for cultivation of highly productive varieties and hybrids in crop production of Ukraine.
Показать больше [+] Меньше [-]Forming and maintaining a collection of plant genetic resources of the Buckwheat (Fagopyrum Mill.) genus Полный текст
2016
Тригуб, О. В
Forming and maintaining a collection of plant genetic resources of the Buckwheat (Fagopyrum Mill.) genus Полный текст
2016
Тригуб, О. В
Purpose. Providing information on the formation, composition, maintenance of the collection of Buckwheat (Fagopyrum Mill.) genus at Ustymivka Experimental Station of Plant Production (Poltava region), and areas of its use: introduction, preservation and study of the material, for the set of index, formation of special collections and providing research and education institutions of Ukraine with a valuable original material. Methods. Methodical developments dealing with formation and maintenance of crop collections, methodical literature on the study of buckwheat collection for breeding and agronomic performance. Results. The author represented results of the collection material study for the main breeding and economically valuable traits including yield, productivity, resistance to abiotic and biotic factors of the environment. The results of work are considered aimed on creating special collections of the gene pool – base, trait (for yield and large seeds; for productivity, drought and heat resistance; for suitability for mechanical cultivation), educational and core ones. Conclusions. All formed and registered collections are a valuable base material for solving a number of breeding goals to increase the quantity and improve the quality of plant production.
Показать больше [+] Меньше [-]Морфо-біологічні ознаки та продуктивність ліній і гібридів суперсолодкої кукурудзи, отриманих на основі мутації sh2 Полный текст
2020
О. Ю. Куліш | М. Ф. Парій
Мета. Суперсолодка кукурудза містить ген, експресія якого зумовлює підвищення концентрації цукру. Ідентифіковано гени ендосперму, що регулюють підвищення вмісту цукру і зменшення вмісту крохмалю; найефективнішими з них були shrunken2 (sh2), brittle1 (bt), sugary1 (su1) і sugary enhancer1 (se). Рецесивна мутація shrunken2 (sh2) забезпечує високий уміст цукрів у зерні молочно-воскової стиглості порівняно з іншими мутантними формами, що безпосередньо впливає на смакові якості sh2-гібридів, обумовлює широке використання цієї мутації в селекційній роботі і має найбільший комерційний потенціал. Основним завданням селекції є створення нового покоління високопродуктивних гібридів, яке залежить від наявності високоякісного вихідного селекційного матеріалу. Тому метою роботи було оцінити лінії суперсолодкої кукурудзи за морфо-біологічними ознаками, виділити ліпші лінії-донори генетичних ознак, створити високопродуктивні гібриди кукурудзи. Методи. Морфо-біологічні ознаки досліджених ліній оцінювали за Класифікатором-довідником виду Zea mays L. Комплексний аналіз зразків за господарсько-цінними ознаками проводили згідно з Методичними рекомендаціями для польового та лабораторного вивчення генетичних ресурсів кукурудзи. Уміст редукувальних цукрів у зерні досліджених ліній і гібридів кукурудзи визначали за методом Бертрана. Результати. Досліджено морфо-біологічні ознаки суперсолодкої кукурудзи. Істотні відмінності між дослідженими лініями спостережено за тривалістю вегетаційного періоду, кількістю насінин в одному ряді й кількістю рядів зерен у качані. Виділено лінії-джерела господарсько-цінних ознак для практичної селекції. Проведено схрещування і отримано 50 гібридних комбінацій, серед яких виділено 16, які характеризувалися кращими показниками господарських ознак. Висновки. У результаті аналізу основних морфо-біологічних ознак у гібридних комбінаціях цукрової кукурудзи з мутантним геном sh2 виділено джерела селекційно-цінних ознак. За тривалістю вегетаційного періоду всі лінії було розділено на три групи: ранні – 8 ліній, середні – 6 і пізні – 8 ліній. Установлено, що перспективними донорами продуктивності качана були лінії SH-621, SH-234 і SH-936, висоти рослин – SH-234 і SH-936, кількості рядів зерен – SH-234 і SH-113, довжини качана – SH-318 і SH-936. За оцінкою морфо-біологічних ознак виділено ліпші лінії, які були схрещені, отримано низку гібридів. Найкращі з них – ‘Матір Драконів F1’, ‘Юрмала F1’ і ‘Ларус F1’ – передано на державну реєстрацію.
Показать больше [+] Меньше [-]Cis-, intra-, subgenesis, genome editing as modern technologies for modifying the crop genomes (review) Полный текст
2016
Волкова, Н. Е
Cis-, intra-, subgenesis, genome editing as modern technologies for modifying the crop genomes (review) Полный текст
2016
Волкова, Н. Е
Purpose. Reviewing the literature on modern technologies of genetic modification of crop genomes. Results. The current state of genetically modified plants creation is analyzed. The information on cis-, intra- and subgenic plants and their comparison with transgenic crops is given. Examples of cis- and intragenesis application for improving characteristics of crops are provided. Such state-of-the-art technology of crop genome modification as genome editing is considered. Conclusions. Technologies for producing cis-, intra-, subgenic plants are rapidly developing and resulting in crops of the 21st century that can solve the problem of food provision for a constantly growing world population with the least contrary to the public interest.
Показать больше [+] Меньше [-]Cis-, intra-, subgenesis, genome editing as modern technologies for modifying the crop genomes (review) Полный текст
2016
Н. Е. Волкова
Purpose. Reviewing the literature on modern technologies of genetic modification of crop genomes. Results. The current state of genetically modified plants creation is analyzed. The information on cis-, intra- and subgenic plants and their comparison with transgenic crops is given. Examples of cis- and intragenesis application for improving characteristics of crops are provided. Such state-of-the-art technology of crop genome modification as genome editing is considered. Conclusions. Technologies for producing cis-, intra-, subgenic plants are rapidly developing and resulting in crops of the 21st century that can solve the problem of food provision for a constantly growing world population with the least contrary to the public interest.
Показать больше [+] Меньше [-]Effect of sowing time on productivity of fenugreek varieties (Trigonella foenum-graecum L., Trigonella coerulea (Desr.) Ser.) Полный текст
2016
Бобось, І. М
Effect of sowing time on productivity of fenugreek varieties (Trigonella foenum-graecum L., Trigonella coerulea (Desr.) Ser.) Полный текст
2016
Бобось, І. М
Purpose. Determination of sowing time effect on productivity of various fenugreek species in the Forest-Steppe zone of Ukraine. Methods. Field study, laboratory test, analytical approach and statistical evaluation. Results. Effect of sowing time on economic characters of common fenugreek (Trigonella foenum graecum L.) and blue fenugreek (Trigonella coerulea (Desr.) Ser.) was investigated. It was found that sowing time significantly influenced the individual productivity of plants and average yield of their fresh and dry matter. In particular, common and blue fenugreek plants formed not only the most developed vegetation but also higher productivity and fresh and dry matter yield, respectively, due to early-spring sowing. In case of summer sowing, the poorest yield of dry matter per unit was obtained for these species caused by a low fresh yield, though the moisture loss index was lower. Conclusions. Common fenugreek is promising for producing “mushroom grass” spices, and the conveyor supply of production in the Forest-Steep zone of Ukraine can be ensured by the first sowing time (the first or the second decades of April) and the second sowing time (the third decade of April) as during this period more developed vegetation is formed that favored the higher yield of dry matter (1,3–1,5 t/ha).
Показать больше [+] Меньше [-]Evaluation of pea varieties based on correlation of quantitative traits and indices Полный текст
2016
О. І. Присяжнюк | Л. В. Король
Purpose. To evaluate pea plants productivity and determine the degree of correlation among the main agronomic characters in pea varieties. Methods. Structural and statistical analysis. Results. Evaluation of pea samples in terms of indices of plant productivity elements level based on correlation analysis and single-factor indices appliance provided insight into the ratio of one trait share per unit of another one. It was defined that some correlations among the elements of productivity was not only moderate and weak, but they also changed their sign that could be the evidence of growth conditions influence on structural relationships between some traits and, consequently, redistribution of their contributions to the formation of variety productivity. Positive and very close relationship of many traits was revealed, particularly between plant height and the height of the plant up to the first bean, the number of nodes and the number of sterile nodes (r = 0,95–0,97). Methodological aspects of the variety model creation were considered, that may be useful not only in pea breeding but also for improving the technology of its cultivation. Conclusions. Correlation relationships were established between the number of beans and the number of fruiting nodes and the number of carpophores containing 2 beans (r = 0,86–0,88), seed mass and plant mass (r = 0,81), the number of seeds per plant and plant mass and seed mass per plant (r = 0,78–0,81), the number of certified seeds and the number of seeds per plant (r = 0,84), the average number of beans per fertile node and the number of carpophores containing 2 beans (r = 0,74) that makes it possible to use them in assessing the productivity of plants.
Показать больше [+] Меньше [-]Allelic status of key genes of carotenogenesis on DNA-markers in maize lines and their hybrids Полный текст
2016
Гончаров, Ю. О | Сатарова, Т. М | Дзюбецький, Б. В | Черчель, В. Ю
Allelic status of key genes of carotenogenesis on DNA-markers in maize lines and their hybrids Полный текст
2016
Гончаров, Ю. О | Сатарова, Т. М | Дзюбецький, Б. В | Черчель, В. Ю
Purpose. The analysis of allelic status of such key genes of carotenogenesis as gene of lycopene-ε-cyclase (lcyε) and gene of β-carotene hydroxylase (crtRB1) for DNA-markers in domestic maize lines and their hybrids. Methods. DNA isolation, PCR, gel electrophoresis. Results. Allelic status of key genes of carotenogenesis was investigated in eight maize inbred lines and their single crosses. Molecular genetic polymorphism in the studied sample of maize lines and hybrids has been detected in gene of β-carotene hydroxylase for marker crtRB1-3’TE. For this gene, codominant character of inheritance of alleles of parental lines in single crosses was confirmed. For markers of gene of lycopene-ε-cyclase lcyε-3’INDL and lcyε-SNP216, polymorphism in the group of investigated lines and hybrids has not been identified, genotypes included only one variant of alleles for each marker. For lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’, the decrease of the activity of β-carotene hydroxylase owing to the mutation of gene crtRB1 under the influence of transposone element at the 3’-end, the inhibition of β-carotene transition into β-cryptoxanthin can be expected, that allows to predict β-carotene accumulation in grain. Conclusions. The study of allelic status of carotenegenesis gene of lycopene-ε-cyclase in maize showed no polymorphism for markers lcyε-3’INDL and lcyε-SNP216 in eight inbred lines and their single crosses, along with this, for marker lcyε-3’INDL in genomes of all studied samples the allele was identified to be favorable for the accumulation of β-carotene. For marker crtRB1-3’TE of gene of β-carotene hydroxylase, the studied breeding material was polymorphic. Allele of crtRB1 being favorable for the accumulation of β-carotene was identified in lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’. Single crosses inherit maternal and paternal alleles of gene of β-carotene hydroxylase codominantly.
Показать больше [+] Меньше [-]Allelic status of key genes of carotenogenesis on DNA-markers in maize lines and their hybrids Полный текст
2016
Ю. О. Гончаров | Т. М. Сатарова | Б. В. Дзюбецький | В. Ю. Черчель
Purpose. The analysis of allelic status of such key genes of carotenogenesis as gene of lycopene-ε-cyclase (lcyε) and gene of β-carotene hydroxylase (crtRB1) for DNA-markers in domestic maize lines and their hybrids. Methods. DNA isolation, PCR, gel electrophoresis. Results. Allelic status of key genes of carotenogenesis was investigated in eight maize inbred lines and their single crosses. Molecular genetic polymorphism in the studied sample of maize lines and hybrids has been detected in gene of β-carotene hydroxylase for marker crtRB1-3’TE. For this gene, codominant character of inheritance of alleles of parental lines in single crosses was confirmed. For markers of gene of lycopene-ε-cyclase lcyε-3’INDL and lcyε-SNP216, polymorphism in the group of investigated lines and hybrids has not been identified, genotypes included only one variant of alleles for each marker. For lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’, the decrease of the activity of β-carotene hydroxylase owing to the mutation of gene crtRB1 under the influence of transposone element at the 3’-end, the inhibition of β-carotene transition into β-cryptoxanthin can be expected, that allows to predict β-carotene accumulation in grain. Conclusions. The study of allelic status of carotenegenesis gene of lycopene-ε-cyclase in maize showed no polymorphism for markers lcyε-3’INDL and lcyε-SNP216 in eight inbred lines and their single crosses, along with this, for marker lcyε-3’INDL in genomes of all studied samples the allele was identified to be favorable for the accumulation of β-carotene. For marker crtRB1-3’TE of gene of β-carotene hydroxylase, the studied breeding material was polymorphic. Allele of crtRB1 being favorable for the accumulation of β-carotene was identified in lines ‘DK253ZSZM’, ‘DK633/266zS,zM’, ‘DK366zS,zM’ and hybrids ‘DK296S×DK253ZSZM’, ‘DK272S×DK633/266zS,zM’ and ‘DK231S×DK366zS,zM’. Single crosses inherit maternal and paternal alleles of gene of β-carotene hydroxylase codominantly.
Показать больше [+] Меньше [-]Adaptive capacity and potential properties of varieties bred at the Potato Research Institute of NAAS Полный текст
2016
Борівський, А. Ф
Adaptive capacity and potential properties of varieties bred at the Potato Research Institute of NAAS Полный текст
2016
Борівський, А. Ф
Purpose. To establish adaptive capacity and reproduction time of the registered potato varieties elite bred at the Potato Research Institute of NAAS of Ukraine in various edaphic and climatic zones of Ukraine. Methods. Field and laboratory tests, statistical evaluation. Results. Potato varieties were tested in the Forest-Seppe, Polissia and Steppe zones of Ukraine. Over the years of testing (2012–2014), the highest yield of potatoes in the south-western part of the Forest-Steppe zone was produced by the early varieties: ‘Kimmeria’ – 46.9 t/ha, ‘Vedruska’ – 38.6 t/ha; middle-early varieties: ‘Partner’ – 33.2 t/ha, ‘Fantazia – 27.7 t/ha; mid-season varieties: ‘Okolytsia’ – 35.4 t/ha, ‘Mandrivnytsia’ – 25.1 t/ha. In the Central Polissia, ‘Kimmeria’ (31 t/ha) was the most productive early variety, the yield at the level of 27.6–29.2 t/ha was formed by such varieties as ‘Skarbnytsia’, ‘Serpanok’, ‘Radynka’, ‘Tyras’. Among the middle-early varieties, the yield of 33.1 t/ha was obtained from the variety ‘Partner’. In the conditions of Steppe zone, such early potato varieties as ‘Vymir’, ‘Glazurna’, ‘Strumok’, ‘Kimmeria’, ‘Svitoch’ and the middle-early variety ‘Ariya’ had the highest productivity in case of growing as bivoltine crop under irrigated conditions. It was found that in the Forest-Steppe and Polissia zones such varieties as ‘Kimmeria’, ‘Vedruska, ‘Serpanok’, ‘Skarbnytsia, ‘Tyras’, ‘Radynka’, ‘Partner’, ‘Slovianka’, ‘Vernisazh’, ‘Mandrivnytsia’, ‘Poliske dzherelo’ and ‘Chervona ruta’ were characterized by high resistance to degeneration (viral diseases and loss of productivity of potato elite in the process of reproduction). According to the results of evaluation of the impact of elite potato reproduction time on affection by viral diseases, optimal time for elite renovation for investigated varieties was determined. Conclusions. In the process of testing in various edaphic and climatic zones of Ukraine, stable yield with a high seed tubers number, regardless of weather conditions during the growing season in the Forest-Steppe and Polissia zones, was formed by some early potato varieties (‘Kimmeria’, ‘Vedruska’, ‘Radynka) and middle-early one (‘Partner’). Among mid-season varieties ‘Vernisazh’, ‘Okolytsia’, ‘Mandrivnytsia’ were identified for high productivity, and among middle-late – ‘Poliske dzherelo’ and ‘Chervona ruta’. The most common viral diseases of potatoes were rugose mosaic and mosaic leaf curling. High efficiency of the use of bivoltine crop method for potato growing to obtain seed material in the Southern Steppe zone of Ukraine has been proved.
Показать больше [+] Меньше [-]Adaptive capacity and potential properties of varieties bred at the Potato Research Institute of NAAS Полный текст
2016
А. Ф. Борівський
Purpose. To establish adaptive capacity and reproduction time of the registered potato varieties elite bred at the Potato Research Institute of NAAS of Ukraine in various edaphic and climatic zones of Ukraine. Methods. Field and laboratory tests, statistical evaluation. Results. Potato varieties were tested in the Forest-Seppe, Polissia and Steppe zones of Ukraine. Over the years of testing (2012–2014), the highest yield of potatoes in the south-western part of the Forest-Steppe zone was produced by the early varieties: ‘Kimmeria’ – 46.9 t/ha, ‘Vedruska’ – 38.6 t/ha; middle-early varieties: ‘Partner’ – 33.2 t/ha, ‘Fantazia – 27.7 t/ha; mid-season varieties: ‘Okolytsia’ – 35.4 t/ha, ‘Mandrivnytsia’ – 25.1 t/ha. In the Central Polissia, ‘Kimmeria’ (31 t/ha) was the most productive early variety, the yield at the level of 27.6–29.2 t/ha was formed by such varieties as ‘Skarbnytsia’, ‘Serpanok’, ‘Radynka’, ‘Tyras’. Among the middle-early varieties, the yield of 33.1 t/ha was obtained from the variety ‘Partner’. In the conditions of Steppe zone, such early potato varieties as ‘Vymir’, ‘Glazurna’, ‘Strumok’, ‘Kimmeria’, ‘Svitoch’ and the middle-early variety ‘Ariya’ had the highest productivity in case of growing as bivoltine crop under irrigated conditions. It was found that in the Forest-Steppe and Polissia zones such varieties as ‘Kimmeria’, ‘Vedruska, ‘Serpanok’, ‘Skarbnytsia, ‘Tyras’, ‘Radynka’, ‘Partner’, ‘Slovianka’, ‘Vernisazh’, ‘Mandrivnytsia’, ‘Poliske dzherelo’ and ‘Chervona ruta’ were characterized by high resistance to degeneration (viral diseases and loss of productivity of potato elite in the process of reproduction). According to the results of evaluation of the impact of elite potato reproduction time on affection by viral diseases, optimal time for elite renovation for investigated varieties was determined. Conclusions. In the process of testing in various edaphic and climatic zones of Ukraine, stable yield with a high seed tubers number, regardless of weather conditions during the growing season in the Forest-Steppe and Polissia zones, was formed by some early potato varieties (‘Kimmeria’, ‘Vedruska’, ‘Radynka) and middle-early one (‘Partner’). Among mid-season varieties ‘Vernisazh’, ‘Okolytsia’, ‘Mandrivnytsia’ were identified for high productivity, and among middle-late – ‘Poliske dzherelo’ and ‘Chervona ruta’. The most common viral diseases of potatoes were rugose mosaic and mosaic leaf curling. High efficiency of the use of bivoltine crop method for potato growing to obtain seed material in the Southern Steppe zone of Ukraine has been proved.
Показать больше [+] Меньше [-]Varietal features of lectin activity of soft winter wheat (Triticum aestivum L.) at the early stages of ontogenesis Полный текст
2016
Чеботарьова, Л. В
Varietal features of lectin activity of soft winter wheat (Triticum aestivum L.) at the early stages of ontogenesis Полный текст
2016
Чеботарьова, Л. В
Purpose. To study the dynamics of soft winter wheat lectin activity in caryopsis and at the early stages of ontogenesis of varieties developed by Poltava State Agrarian Academy as well as investigate varietal differences depending on dynamic pH medium. Methods. Laboratory analysis, method of hemagglutination reaction, analytical approach, statistical evaluation. Results. A change in lectin activity was determined in samples of 14 soft winter wheat varieties in caryopsis and at the early stages of ontogenesis (on the third, seventh, tenth and twelfth day of seedlings emergence). High lectin activity was observed in samples of seven and ten days seedlings. This could be evidence of active participation of proteins in physiological and biochemical processes at the time of emerging wheat tillering node that can be important in forming adaptive reactions to stress. Wheat lectin activity variation for рН ranging between 4,0 and 8,0 that depended on a variety was defined for the first time. Conclusions. According to hemagglutination ability of wheat lectins, all varieties were divided into three groups: of high ability – 4 varieties, middle – 9 varieties, low – 1 variety. Varietal variability for the investigated trait can be used in breeding programs as marker trait. In most varieties variation of hemagglutination was observed at dilutions ranging between 1:2 and 1:256. Availability of two lectin agglutination peaks in acidic and alkaline media for seedlings was fixed.
Показать больше [+] Меньше [-]Varietal features of lectin activity of soft winter wheat (Triticum aestivum L.) at the early stages of ontogenesis Полный текст
2016
Л. В. Чеботарьова
Purpose. To study the dynamics of soft winter wheat lectin activity in caryopsis and at the early stages of ontogenesis of varieties developed by Poltava State Agrarian Academy as well as investigate varietal differences depending on dynamic pH medium. Methods. Laboratory analysis, method of hemagglutination reaction, analytical approach, statistical evaluation. Results. A change in lectin activity was determined in samples of 14 soft winter wheat varieties in caryopsis and at the early stages of ontogenesis (on the third, seventh, tenth and twelfth day of seedlings emergence). High lectin activity was observed in samples of seven and ten days seedlings. This could be evidence of active participation of proteins in physiological and biochemical processes at the time of emerging wheat tillering node that can be important in forming adaptive reactions to stress. Wheat lectin activity variation for рН ranging between 4,0 and 8,0 that depended on a variety was defined for the first time. Conclusions. According to hemagglutination ability of wheat lectins, all varieties were divided into three groups: of high ability – 4 varieties, middle – 9 varieties, low – 1 variety. Varietal variability for the investigated trait can be used in breeding programs as marker trait. In most varieties variation of hemagglutination was observed at dilutions ranging between 1:2 and 1:256. Availability of two lectin agglutination peaks in acidic and alkaline media for seedlings was fixed.
Показать больше [+] Меньше [-]Assessment of combining ability for grain yield of self-pollinated S5 maize (Zea mays L.) families of mixed germplasm Полный текст
2016
Гайдаш, О. Л
Assessment of combining ability for grain yield of self-pollinated S5 maize (Zea mays L.) families of mixed germplasm Полный текст
2016
Гайдаш, О. Л
Purpose. Assessment and selection of self-pollinated S5 maize (Zea mays L.) families with high combining ability and wide adaptive capacity obtained on the basis of specially produced hybrids by crossing elite lines of various genetic plasms with the best main agronomic characters. Methods. Field studies, mathematico-statistical evaluation. Results. An amplitude of grain yield ranging from 5.96–10.96 t/ha (x = 8.44 t/ha) in 2013 to 2.67–7.59 t/ha (x = 5.08 t/ha) in 2014 was determined in the course of study of the testcrosses of self-pollinated S5 families. It was found that different response of genotypes of the studied testcrosses to the year conditions significantly affected the average yield level, which decreased in the stressful 2014 by 3.4 t/ha as compared to 2013. The results of the assessment based on the general and specific combining ability of new parent material of mixed germplasm were shown. A significant variability of the estimates of GCA (general combining ability) effects depending on the year conditions was observed in the course of study. A marked difference in the estimates of GCA effects based on the grain yield was revealed. They were persistently high in 19% of the best self-pollinated families in both years, 14% of the families had persistently low estimates of GCA effects, 67% changed their value depending on the genotype and year conditions. Conclusions. 17 best self-pollinated families with persistently high estimates of GCA, 6 families featuring high tolerance to drought and 4 families with persistently high variances of SCA were selected. The selected families will be used as a parent material in selection programs aimed to create new high performance hybrids.
Показать больше [+] Меньше [-]Characteristics of productivity of soft winter wheat samples from Common Bunt-Resistant Nursery (CBUNT-RES) in the southern Forest-Steppe zone of Ukraine Полный текст
2020
С. М. Холод | В. М. Кір’ян | Р. С. Вискуб
Purpose. Evaluate the introduced samples of soft winter wheat from the international nursery Common Bunt-Resistant Nursery (CBUNT-RES) of different ecological and geographical origin in the southern part of the Forest-Steppe zone of Ukraine according to a set of productivity indicators in order to define the most valuable samples and describe them. Methods. During 2016–2019 on the base of Ustymivka Experimental Station for Plant Production of the V. Ya. Yuriev Plant Production Institute of NAAS the authors studied, evaluated and described 75 new soft winter wheat samples of various eco-geographical origins by productivity traits. In the field and laboratory conditions, indicators of yield and productivity were determined: 1000 grains weight, plant height and ear length, the number of spikelets and grains in the ear, weight of ear and grain and early-ripening. Results. Plant material with increased parameters of economic and biological characters was identified. During studies, it was found that ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘91-142A61/KATIA1//GRISET-4’, ‘SAULESKU#44/TR810200//GRISET-4’, ‘ATTILA/BABAX//PASTOR/4/…’ (IU067591) (Turkey), ‘INTENSIVNAYA//PBW343*2/TUKURU’, ‘SANZAR-8/KKTS’ (Mexico) were the high-yielding varieties. The samples of soft winter wheat as ‘F08347G8’, ‘F00628G34-1’ (Romania), ‘INTENSIVNAYA//PBW343*2/…’ (IU067637) (Mexico), ‘ATAY/GALVEZ87/6/TAST/…’ (IU067587), ‘DE9/MERCAN-2’, ‘KRASNODAR/FRTL/6…’ (IU067595), ‘SAULESKU#44/TR810200//GRISET-4’, ‘GANSU-1/3/AUSGS50AT34/…’ (IU067598), ‘ORKINOS-1*2/3/AUS…’ (IU067608), ‘KAMBARA1/ZANDER-17’ , ‘TAM200/KAUZ/4/CHAM6//…’ (IU067612) (Turkey) contained a large grain weight (more than 5.0 g), plant productivity in these samples was rather high due to increased amount of grains and the thousand kernel weight. Conclusions. The introduced soft winter wheat samples of various eco-geographical origins were adapted to the Southern Forest-Steppe and can be recommended as a source material in breeding to increasing productive capacity.
Показать больше [+] Меньше [-]Methodical aspects of assessing grape breeding material Полный текст
2016
Л. В. Герус
Purpose. Developing methodical aspects of assessing grape breeding material in hybrid combinations for a number of indicators of adaptability and productivity. Methods. Breeding, hybridological, statistical ones. Results. Main requirements were determined for a new generation of table and technical grapes that was based on the set breeding task and patent searches, literary sources and Internet resources. 12 main indicators of adaptability, productivity and quality of products were analyzed, a high level of their display is required for pre-selection of grape seedlings for further study. Gradation from the lowest level of display of the above economic characters of grapes to the medium and high ones was developed. For their definition points-based system is used – the lowest level of display corresponds to one and three points, average – five, and high – seven and nine. As a result, studied plants can score 12 to 108 points. Variation in the plant evaluation over the years should not exceed 10 points. A seedling cannot be selected by the maximum level of displaying only one parameter, in the same manner as it shall be rejected with a minimum level of display of any of the said indicators. Seedlings that display a stable middle level of traits can be placed to the breeding nursery to study the level of display of the essential parameters and properties of the grafted culture. Conclusions. Criteria and basic parameters of genotypic values in hybrid combinations were determined. Plants that have been estimated in 60 points or higher may be considered as promising ones, that is those which require further studying. Valuable genotypes correspond to the level of 80–100, very valuable – of 101–108 points.
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