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Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators Полный текст
2020
Peluso, Julieta | Aronzon, Carolina Mariel | Ríos de Molina, María del Carmen | Rojas, Dante Emanuel | Cristos, Diego Sebastian | Pérez Coll, Cristina Silvia
Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators Полный текст
2020
Peluso, Julieta | Aronzon, Carolina Mariel | Ríos de Molina, María del Carmen | Rojas, Dante Emanuel | Cristos, Diego Sebastian | Pérez Coll, Cristina Silvia
The Paraná River basin is one of the most important in South America and is affected by human activities that take place on its margins. In particular, the De la Cruz stream flows through an industrial pole and the Arrecifes River goes mainly through agricultural fields. The aim of this study was to evaluate the water quality of the De la Cruz stream (S1) and the Arrecifes River (S2) by means of physicochemical parameters, including metals and pesticides concentrations. Since amphibians are good indicators of environmental quality, bioassays with Rhinella arenarum were carried on. For lethal and sublethal parameters, embryos and larvae were exposed to a dilution gradient of water samples and AMPHITOX Solution (AS) as negative control for 504 h. For the determination of oxidative stress biomarkers (Catalase -CAT-, Glutathione S-Transferase -GST-, Reduced Glutathione -GSH-, and lipid peroxidation -TBARS-), embryos and larvae were exposed to undiluted water samples and AS. For the determination of micronuclei, larvae at hind limb bud stage (S.28) were exposed to undiluted water samples, simultaneously with negative and positive controls (AS and cyclophosphamide 40 mg/L, respectively). Dissolved oxygen was low in both sites and the copper levels exceeded the Argentine limit for the protection of aquatic life. In embryos exposure, water sample from S1 caused lethal effects (504h-LC50 = 49 (28–71.6)%), increased TBARS levels, and GST and CAT activities. In larvae exposure, water sample from this site decreased CAT activity, while the water sample from S2 caused important lethal effects (504h-LC50 = 98.72 (60.60–302.52)%), low GSH levels and increased GST activity. Water samples from both sites induced higher micronuclei frequency than the negative control. This study alerts about the degradation of water quality of the studied sites including lethal and sublethal effects in R. arenarum that can jeopardize the native populations of this species. | Fil: Peluso, Julieta. Universidad Nacional de San Martín (UNSAM). Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | Fil: Aronzon, Carolina Mariel. Universidad Nacional de San Martín (UNSAM). Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. | Fil: Ríos de Molina, María del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | Fil: Rojas, Dante Emanuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica; Argentina. | Fil: Cristos, Diego Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica; Argentina. | Fil: Pérez Coll, Cristina Silvia. Universidad Nacional de San Martín (UNSAM). Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Показать больше [+] Меньше [-]Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators Полный текст
2020
Peluso, Julieta | Aronzon, Carolina M. | Ríos de Molina, María del Carmen | Rojas, Dante E. | Cristos, Diego | Pérez Coll, Cristina S.
The Paraná River basin is one of the most important in South America and is affected by human activities that take place on its margins. In particular, the De la Cruz stream flows through an industrial pole and the Arrecifes River goes mainly through agricultural fields. The aim of this study was to evaluate the water quality of the De la Cruz stream (S1) and the Arrecifes River (S2) by means of physicochemical parameters, including metals and pesticides concentrations. Since amphibians are good indicators of environmental quality, bioassays with Rhinella arenarum were carried on. For lethal and sublethal parameters, embryos and larvae were exposed to a dilution gradient of water samples and AMPHITOX Solution (AS) as negative control for 504 h. For the determination of oxidative stress biomarkers (Catalase -CAT-, Glutathione S-Transferase -GST-, Reduced Glutathione -GSH-, and lipid peroxidation -TBARS-), embryos and larvae were exposed to undiluted water samples and AS. For the determination of micronuclei, larvae at hind limb bud stage (S.28) were exposed to undiluted water samples, simultaneously with negative and positive controls (AS and cyclophosphamide 40 mg/L, respectively). Dissolved oxygen was low in both sites and the copper levels exceeded the Argentine limit for the protection of aquatic life. In embryos exposure, water sample from S1 caused lethal effects (504h-LC50 = 49 (28–71.6)%), increased TBARS levels, and GST and CAT activities. In larvae exposure, water sample from this site decreased CAT activity, while the water sample from S2 caused important lethal effects (504h-LC50 = 98.72 (60.60–302.52)%), low GSH levels and increased GST activity. Water samples from both sites induced higher micronuclei frequency than the negative control. This study alerts about the degradation of water quality of the studied sites including lethal and sublethal effects in R. arenarum that can jeopardize the native populations of this species.
Показать больше [+] Меньше [-]Gammarid exposure to pyriproxyfen and/or cadmium: what effects on the methylfarnesoate signalling pathway? Полный текст
2020
Jaegers, Jeremy | Gismondi, Eric | FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège
Gammarid exposure to pyriproxyfen and/or cadmium: what effects on the methylfarnesoate signalling pathway? Полный текст
2020
Jaegers, Jeremy | Gismondi, Eric | FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège
peer reviewed | Due to expected changes in climate, it is predicted that disease-carrying mosquitoes will expand their geographical range, resulting in increased use of insect growth regulators (IGRs) to face their proliferation. Among IGRs, pyriproxyfen (PXF) is widely used and has been shown to prevent larvae from developing into adults, rendering them unable to reproduce. However, because of the similarity of crustacean and insect endocrine systems, PXF could also impact aquatic crustaceans. In addition, when spreading in the environment, PXF is found in a mixture with other pollutants such as metallic trace elements, which could alter its effect. Consequently, the present work was devoted to analysing the effects of PXF on the methylfarnesoate (MF) hormonal pathway of the freshwater amphipod Gammarus pulex, as well as its combined binary effects with cadmium (Cd), by measuring MF concentration, as well as the relative transcriptional expression of the farnesoic acid O-methyltransferase (FAMeT) (enzyme limiting the MF production), the methoprene-tolerant receptor (Met), and the broad-complex (BrC) as a transcription factor. Results revealed that single exposures to PXF or Cd have mainly overexpressed FAMeT, Met, and BrC but did not significantly affect MF concentration. Conversely, the mixture exposures seemed to suppress these effects and even achieve antagonistic effects. This work confirmed that PXF single exposure could impact non-target organisms such as amphipods through changes in hormonal pathways of MF. In the same way, it highlighted that Cd could also impair the endocrine system of exposed organisms. However, antagonistic effects have been observed in exposure to mixtures, suggesting different long-term consequences on the growth of amphipods under realistic exposure conditions.
Показать больше [+] Меньше [-]Gammarid exposure to pyriproxyfen and/or cadmium: what effects on the methylfarnesoate signalling pathway? Полный текст
2020
Due to expected changes in climate, it is predicted that disease-carrying mosquitoes will expand their geographical range, resulting in increased use of insect growth regulators (IGRs) to face their proliferation. Among IGRs, pyriproxyfen (PXF) is widely used and has been shown to prevent larvae from developing into adults, rendering them unable to reproduce. However, because of the similarity of crustacean and insect endocrine systems, PXF could also impact aquatic crustaceans. In addition, when spreading in the environment, PXF is found in a mixture with other pollutants such as metallic trace elements, which could alter its effect. Consequently, the present work was devoted to analysing the effects of PXF on the methylfarnesoate (MF) hormonal pathway of the freshwater amphipod Gammarus pulex, as well as its combined binary effects with cadmium (Cd), by measuring MF concentration, as well as the relative transcriptional expression of the farnesoic acid O-methyltransferase (FAMeT) (enzyme limiting the MF production), the methoprene-tolerant receptor (Met), and the broad-complex (BrC) as a transcription factor. Results revealed that single exposures to PXF or Cd have mainly overexpressed FAMeT, Met, and BrC but did not significantly affect MF concentration. Conversely, the mixture exposures seemed to suppress these effects and even achieve antagonistic effects. This work confirmed that PXF single exposure could impact non-target organisms such as amphipods through changes in hormonal pathways of MF. In the same way, it highlighted that Cd could also impair the endocrine system of exposed organisms. However, antagonistic effects have been observed in exposure to mixtures, suggesting different long-term consequences on the growth of amphipods under realistic exposure conditions.
Показать больше [+] Меньше [-]Early-life exposure to artificial light at night elevates physiological stress in free-living songbirds Полный текст
2020
Grunst, Melissa | Raap, Thomas | Grunst, Andrea | Pinxten, Rianne | Parenteau, Charline | Angelier, Frédéric | Eens, Marcel | Department of Biology, Behavioural Ecology and Ecophysiology Group ; University of Antwerp (UA) | Department of Biology (Ethology) ; University of Antwerp (UA) | Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) ; La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Department of Biology (Ethology) ; University of Antwerp (UA) | ANR-16-CE02-0004,URBASTRESS,Influence de l'urbanisation sur les populations de vertébrés: une approche éco-physiologique(2016)
International audience | Artificial light at night (ALAN) can disrupt adaptive patterns of physiology and behavior that promote high fitness, resulting in physiological stress and elevation of steroid glucocorticoids (corticosterone, CORT in birds). Elevated CORT may have particularly profound effects early in life, with the potential for enduring effects that persist into adulthood. Research on the consequences of early-life exposure to ALAN remains limited, especially outside of the laboratory, and the effects of early-life light exposure on CORT concentrations in wild nestling birds remain to be elucidated. We used an experimental setup to test the hypothesis that ALAN elevates CORT concentrations in developing free-living birds, by exposing nestling great tits (Parus major) to ALAN inside nest boxes. We measured CORT in feathers grown over the timeframe of the experiment (7 nights), such that CORT concentrations represent an integrative metric of hormone release over the period of nocturnal light exposure, and of development. We also assessed the relationships between feather CORT concentrations, body condition, nestling size rank and fledging success. In addition, we evaluated the relationship between feather CORT concentrations and telomere length. Nestlings exposed to ALAN had higher feather CORT concentrations than control nestlings, and nestlings in poorer body condition and smaller brood members also had higher CORT. On the other hand, telomere length, fledging success, and recruitment rate were not significantly associated with light exposure or feather CORT concentrations. Results indicate that exposure to ALAN elevates CORT concentrations in nestlings, which may reflect physiological stress. In addition, the organizational effects of CORT are known to be substantial. Thus, despite the lack of effect on telomere length and survivorship, elevated CORT concentrations in nestlings exposed to ALAN may have subsequent impacts on later-life fitness and stress sensitivity.
Показать больше [+] Меньше [-]A comprehensive framework for assessing the impact of potential agricultural pollution on grain security and human health in economically developed areas Полный текст
2020
Jiang, Yefeng | Chen, Songchao | Hu, Bifeng | Zhou, Yin | Liang, Zongzheng | Jia, Xiaolin | Huang, Mingxiang | Wei, Jing | Shi, Zhou | Zhejiang University [Hangzhou, China] | InfoSol (InfoSol) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Information Center of Ministry of Ecology and Environment | Beijing Normal University (BNU) | University of Maryland [Baltimore] (UMB)
International audience | Agricultural pollution poses a considerable challenge to grain security and human health, especially in economically developed areas. Mineral exploitation, chemical enterprise operation, pesticide and fertilizer application, sewage discharge, and vehicle emissions are the pollution sources of agricultural land. Identifying and assessing potential agricultural pollution (PAP) is, therefore, the most urgent task to achieve grain security and the human health. Large-scale (e.g., regional or national) PAP assessment can be very expensive, which could also generate a certain amount of information that usually discourages evaluation by decision-makers. To identify areas for regional priority investigation, here we proposed an assessment framework for PAP in economically developed areas. The framework consisted of PAP assessment, vulnerability assessment, hazard assessment, and socio-economic assessment. Then, we conducted a case study by using the proposed framework in one of China’s economically developed areas, Zhejiang Province. The results showed that PAP, especially soil heavy metal pollution, soil acidification, and surface water pollution involved almost the entire study area. High-vulnerability high-hazard areas were mainly associated with high socio-economic development or high grain yield. These areas had negatively affected grain security and increased carcinogenic risk, potentially contributing to the formation of cancer villages. Based on the results, we proposed measures for environmental risk managers to alleviate the impact of PAP on grain security and human health in economically developed areas.
Показать больше [+] Меньше [-]Current status, spatial features, health risks, and potential driving factors of soil heavy metal pollution in China at province level Полный текст
2020
Hu, Bifeng | Shao, Shuai | Ni, Hao | Fu, Zhiyi | Hu, Linshu | Zhou, Yin | Min, Xiaoxiao | She, Shufeng | Chen, Songchao | Huang, Mingxiang | Zhou, Lianqing | Li, Yan | Shi, Zhou | Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences ; Zhejiang University [Hangzhou, China] | Unité de Science du Sol (Orléans) (URSols) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Université d'Orléans (UO) | Department of Earth System Science [Tsinghua] ; Tsinghua University [Beijing] (THU) | School of Earth Sciences, Zhejiang University (ZJU) | College of Environmental and Resource Sciences, Zhejiang University | Institute of Land Science and Property, School of Public Affairs ; Zhejiang University [Hangzhou, China] | InfoSol (InfoSol) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Information Center of Ministry of Ecology and Environment | Institute of Agricultural Remote Sensing and Information Technology Application ; Zhejiang University [Hangzhou, China]
International audience | In this study we systematically reviewed 1203 research papers published between 2008 and 2018 in China and recorded related data on eight kinds of soil heavy metals (Cr, Pb, Cd, Hg, As, Cu, Zn, and Ni). Based on that, the pollution levels, ecological risk and health risk caused by soil heavy metals were evaluated and the pollution hot spots and potential driving factors of different heavy metals in different provinces were also identified. Results indicated accumulation of heavy metals in soils of most provinces in China compared with background values. Consistent with previous findings, the most prevalent polluted heavy metals were Cd and Hg. Polluted regions are mainly located in central, southern and southwestern China. Hunan, Guangxi, Yunnan, and Guangdong provinces were the most polluted provinces. For the potential health risk caused by heavy metals pollution, children are more likely confront with non-carcinogenic risk than adults and seniors. And children in Hunan and Guangxi province were experiencing relatively larger non-carcinogenic risk. In addition, children in part of provinces were undergoing potentially carcinogenic risks due to soil heavy metals exposure. Furthermore, in our study the 31 provinces in mainland China were divided into six subsets according to corresponding potential driving factors for heavy metal accumulation. Our study provide more comprehensive and updated information for contributing to better soil management, soil remediation, and soil contamination control in China.
Показать больше [+] Меньше [-]First detection of pectenotoxin-2 in shellfish associated with an intense spring bloom of Dinophysis acuminata on the central Chilean coast Полный текст
2020
Díaz, Patricio A. | Álvarez, Gonzalo | Seguel, Miriam | Marín, Andrés | Krock, Bernd
First detection of pectenotoxin-2 in shellfish associated with an intense spring bloom of Dinophysis acuminata on the central Chilean coast Полный текст
2020
Díaz, Patricio A. | Álvarez, Gonzalo | Seguel, Miriam | Marín, Andrés | Krock, Bernd
Diarrhetic shellfish poisoning (DSP) toxins and pectenotoxins (PTX) produced by endemic species of the genus Dinophysis, mainly D. acuta and D. acuminata, pose a big threat to public health, artisanal fisheries and the aquaculture industry in Southern Chile. This work reports the first detection of lipophilic toxins, including pectenotoxin-2 (PTX-2) and gymnodimine-A (GYM-A), in hard razor clam (Tagelus dombeii) associated with an unprecedented spring bloom −38.4 × 103 cells L−1 in integrated hose sampler (0–10 m) – of Dinophysis acuminata in coastal waters of central Chile. The socio-economic challenges to small-scale fisheries are discussed. The study points to the pressing need for sound policies to face unexpected HAB event, probably due to biogeographical expansions, with a focus on fisheries management, participation of stakeholders, and development of adaptive capacities.
Показать больше [+] Меньше [-]First detection of pectenotoxin-2 in shellfish associated with an intense spring bloom of Dinophysis acuminata on the central Chilean coast Полный текст
2020
Díaz, Patricio A. | Álvarez, Gonzalo | Seguel, Miriam | Marín, Andrés | Krock, Bernd
Diarrhetic shellfish poisoning (DSP) toxins and pectenotoxins (PTX) produced by endemic species of the genus Dinophysis, mainly D. acuta and D. acuminata, pose a big threat to public health, artisanal fisheries and the aquaculture industry in Southern Chile. This work reports the first detection of lipophilic toxins, including pectenotoxin-2 (PTX-2) and gymnodimine-A (GYM-A), in hard razor clam (Tagelus dombeii) associated with an unprecedented spring bloom −38.4 × 10³ cells L⁻¹ in integrated hose sampler (0–10 m) – of Dinophysis acuminata in coastal waters of central Chile. The socio-economic challenges to small-scale fisheries are discussed. The study points to the pressing need for sound policies to face unexpected HAB event, probably due to biogeographical expansions, with a focus on fisheries management, participation of stakeholders, and development of adaptive capacities.
Показать больше [+] Меньше [-]Development and environmental implication of pedotransfer functions of Cd desorption rate coefficients in historically polluted soils Полный текст
2020
Lin, Zhongbing | Zou, Xingying | Zhang, Renduo | Nguyen, Christophe | Huang, Jiesheng | Wang, Kang | Wu, Jingwei | Huang, Shuang | State Key Laboratory of Water Resources and Hydropower Engineering Science ; Wuhan University [China] | Sun Yat-sen University [Guangzhou] (SYSU) | Interactions Sol Plante Atmosphère (UMR ISPA) ; Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
International audience | The desorption rate is an important factor determining cadmium (Cd) ecotoxicity and pollution remediation in soils. The pedotransfer functions (PTFs) of desorption rate coefficients of fresh Cd in soils have been developed in literature. We hypothesized that the aging of Cd pollution would alter Cd desorption process. Taking historically polluted soils as the object, this study aimed at testing the hypothesis and developing new PTFs of desorption rate coefficients for historical Cd. 15 d batch extraction experiments and 13 kinetic models were employed to define Cd desorption rate coefficients in 27 historically polluted soil samples. Compared with fresh Cd, the desorption rate coefficients of historical Cd were lower, and the break time of biphasic desorption processes was retarded to 3 d (4320 min). Different with the usual models for fresh Cd desorption (e.g. parabolic diffusion and two constant rate models), the best models to mimic the historical Cd desorption processes were the pseudo first order, logarithmic, Elovich, and simple Elovich models. The rate-limiting step controlling Cd desorption was changed from the intraparticle diffusion to the interface reaction with aging of pollution. New PTFs of desorption rate coefficients of historical Cd were established (R2 ≥ 0.71). Cd desorption rate coefficients increased with organic matter and clay contents, but decreased with oxalate extractable Fe content, solution pH, cation exchange capacity, and silt content. The key soil properties influencing desorption rate coefficients were not altered by the aging of pollution. The developed PTFs could guide us to adjusting the ecotoxicity and pollution remediation of Cd in historically polluted field soils.
Показать больше [+] Меньше [-]Effects of cadmium, inorganic mercury and methyl-mercury on the physiology and metabolomic profiles of shoots of the macrophyte Elodea nuttallii Полный текст
2020
Cosio, Claudia | Renault, D | Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO) ; Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH) ; Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet ; Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS) | Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO) ; Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) ; Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des sciences de l'environnement de Rennes (OSERen) ; Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Experiments were performed at Geneva University during CC previous position. Authors thank Rebecca Beauvais-Fluck, Floriane Larras, Beatriz Lobo, Nicole Regier and Debora Tanaami for their help in the management of cultures, sampling of water and the preparation of cytosol extracts. The Swiss National Science Foundation (contracts n°205321_138254 and 200020_157173).
Effects of cadmium, inorganic mercury and methyl-mercury on the physiology and metabolomic profiles of shoots of the macrophyte Elodea nuttallii Полный текст
2020
Cosio, Claudia | Renault, D | Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO) ; Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH) ; Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet ; Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS) | Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO) ; Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) ; Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des sciences de l'environnement de Rennes (OSERen) ; Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Experiments were performed at Geneva University during CC previous position. Authors thank Rebecca Beauvais-Fluck, Floriane Larras, Beatriz Lobo, Nicole Regier and Debora Tanaami for their help in the management of cultures, sampling of water and the preparation of cytosol extracts. The Swiss National Science Foundation (contracts n°205321_138254 and 200020_157173).
International audience | Macrophytes are known to bioaccumulate metals, but a thorough understanding of tolerance strategies and molecular impact of metals in aquatic plants is still lacking. The present study aimed to compare Hg and Cd effects in a representative macrophyte, Elodea nuttallii using physiological endpoints and metabolite profiles in shoots and cytosol. Exposure 24 h to methyl-Hg (30 ng L), inorganic Hg (70 ng L) and Cd (280 μg L) did not affect photosynthesis, or antioxidant enzymes despite the significant accumulation of metals, confirming a sublethal stress level. In shoots, Cd resulted in a higher level of regulation of metabolites than MeHg, while MeHg resulted in the largest number of regulated metabolites and IHg treatment regulated no metabolites significantly. In cytosol, Cd regulated more metabolites than IHg and only arginine, histidine and mannose were reduced by MeHg exposure. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of data suggested that exposure to MeHg resulted in biochemical changes including aminoacyl-tRNA biosynthesis, glycine, serine and threonine metabolism, nitrogen metabolism, arginine and proline metabolism, cyanoamino acid metabolism, while the treatment of Cd stress caused significant variations in aminoacyl-tRNA biosynthesis and branched-chain amino acids pathways. Data supports an impact of MeHg on N homeostasis, while Cd resulted in an osmotic stress-like pattern and IHg had a low impact. Marked differences in the responses to MeHg and IHg exposure were evidenced, supporting different molecular toxicity pathways and main impact of MeHg on non-soluble compartment, while main impact of IHg was on soluble compartment. Metabolomics was used for the first time in this species and proved to be very useful to confirm and complement recent knowledge gained by transcriptomics and proteomics, highlighting the high interest of multi-omics approaches to identify early impact of environmental pollution.
Показать больше [+] Меньше [-]Effects of cadmium, inorganic mercury and methyl-mercury on the physiology and metabolomic profiles of shoots of the macrophyte Elodea nuttallii Полный текст
2020
Cosio, Claudia | Renault, David
Macrophytes are known to bioaccumulate metals, but a thorough understanding of tolerance strategies and molecular impact of metals in aquatic plants is still lacking. The present study aimed to compare Hg and Cd effects in a representative macrophyte, Elodea nuttallii using physiological endpoints and metabolite profiles in shoots and cytosol.Exposure 24 h to methyl-Hg (30 ng L⁻¹), inorganic Hg (70 ng L⁻¹) and Cd (280 μg L⁻¹) did not affect photosynthesis, or antioxidant enzymes despite the significant accumulation of metals, confirming a sublethal stress level. In shoots, Cd resulted in a higher level of regulation of metabolites than MeHg, while MeHg resulted in the largest number of regulated metabolites and IHg treatment regulated no metabolites significantly. In cytosol, Cd regulated more metabolites than IHg and only arginine, histidine and mannose were reduced by MeHg exposure. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of data suggested that exposure to MeHg resulted in biochemical changes including aminoacyl-tRNA biosynthesis, glycine, serine and threonine metabolism, nitrogen metabolism, arginine and proline metabolism, cyanoamino acid metabolism, while the treatment of Cd stress caused significant variations in aminoacyl-tRNA biosynthesis and branched-chain amino acids pathways. Data supports an impact of MeHg on N homeostasis, while Cd resulted in an osmotic stress-like pattern and IHg had a low impact. Marked differences in the responses to MeHg and IHg exposure were evidenced, supporting different molecular toxicity pathways and main impact of MeHg on non-soluble compartment, while main impact of IHg was on soluble compartment. Metabolomics was used for the first time in this species and proved to be very useful to confirm and complement recent knowledge gained by transcriptomics and proteomics, highlighting the high interest of multi-omics approaches to identify early impact of environmental pollution.
Показать больше [+] Меньше [-]Cadmium distribution in mature durum wheat grains using dissection, laser ablation-ICP-MS and synchrotron techniques * Полный текст
2020
Yan, Bofang | Isaure, M.-P. | Mounicou, Sandra | Castillo-Michel, Hiram | de Nolf, Wout | Nguyen, Christophe | Cornu, Jean-Yves | Interactions Sol Plante Atmosphère (UMR ISPA) ; Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM) ; Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | European Synchrotron Radiation Facility (ESRF) | CADMIGRAIN project | AQUITRACE project | ANR-15-CE21-0001,CaDON,Cadmium et Deoxynivalenol dans les récoltes de blé dur: comprendre les évènements de contamination croisée et évaluer la toxicité du mélange.(2015)
Cadmium distribution in mature durum wheat grains using dissection, laser ablation-ICP-MS and synchrotron techniques * Полный текст
2020
Yan, Bofang | Isaure, M.-P. | Mounicou, Sandra | Castillo-Michel, Hiram | de Nolf, Wout | Nguyen, Christophe | Cornu, Jean-Yves | Interactions Sol Plante Atmosphère (UMR ISPA) ; Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM) ; Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | European Synchrotron Radiation Facility (ESRF) | CADMIGRAIN project | AQUITRACE project | ANR-15-CE21-0001,CaDON,Cadmium et Deoxynivalenol dans les récoltes de blé dur: comprendre les évènements de contamination croisée et évaluer la toxicité du mélange.(2015)
International audience | Understanding how essential and toxic elements are distributed in cereal grains is a key to improving the nutritional quality of cereal-based products. The main objective of this work was to characterize the distribution of Cd and of nutrients (notably Cu, Fe, Mn, P, S and Zn) in the durum wheat grain. Laser ablation inductively coupled mass spectrometry and synchrotron micro X-ray fluorescence were used for micro-scale mapping of Cd and nutrients. A dissection approach was used to quantitatively assess the distribution of Cd and nutrients among grain tissues. Micro X-ray absorption near-edge spectroscopy was used to identify the Cd chemical environment in the crease. Cadmium distribution was characterized by strong accumulation in the crease and by non-negligible dissemination in the endosperm. Inside the crease, Cd accumulated most in the pigment strand where it was mainly associated with sulfur ligands. High-resolution maps highlighted very specific accumulation areas of some nutrients in the germ, for instance Mo in the root cortex primordia and Cu in the scutellum. Cadmium loading into the grain appears to be highly restricted. In the grain, Cd co-localized with several nutrients, notably Mn and Zn, which challenges the idea of selectively removing Cd-enriched fractions by dedicated milling process.
Показать больше [+] Меньше [-]Cadmium distribution in mature durum wheat grains using dissection, laser ablation-ICP-MS and synchrotron techniques Полный текст
2020
Yan, Bofang | Isaure, Marie-Pierre | Mounicou, Sandra | Castillo-Michel, Hiram | De Nolf, Wout | Nguyen, Christophe | Cornu, Jean-Yves
Understanding how essential and toxic elements are distributed in cereal grains is a key to improving the nutritional quality of cereal-based products. The main objective of this work was to characterize the distribution of Cd and of nutrients (notably Cu, Fe, Mn, P, S and Zn) in the durum wheat grain. Laser ablation inductively coupled mass spectrometry and synchrotron micro X-ray fluorescence were used for micro-scale mapping of Cd and nutrients. A dissection approach was used to quantitatively assess the distribution of Cd and nutrients among grain tissues. Micro X-ray absorption near-edge spectroscopy was used to identify the Cd chemical environment in the crease. Cadmium distribution was characterized by strong accumulation in the crease and by non-negligible dissemination in the endosperm. Inside the crease, Cd accumulated most in the pigment strand where it was mainly associated with sulfur ligands. High-resolution maps highlighted very specific accumulation areas of some nutrients in the germ, for instance Mo in the root cortex primordia and Cu in the scutellum. Cadmium loading into the grain appears to be highly restricted. In the grain, Cd co-localized with several nutrients, notably Mn and Zn, which challenges the idea of selectively removing Cd-enriched fractions by dedicated milling process.
Показать больше [+] Меньше [-]Associations between persistent organic pollutants and endometriosis: A multipollutant assessment using machine learning algorithms Полный текст
2020
Matta, Komodo | Vigneau, Evelyne | Cariou, Véronique | Mouret, Delphine | Ploteau, Stéphane | Le Bizec, Bruno | Antignac, Jean-Philippe | Cano-Sancho, Germán | Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA) ; École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Statistique, Sensométrie et Chimiométrie (StatSC) ; École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Centre hospitalier universitaire de Nantes (CHU Nantes)
International audience
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