The intestinal microbiota has a key role in human health via the interaction with the somatic and immune cells in the digestive tract environment. Food, through matrix effect, nutrient and non-nutrient molecules, is a key regulator of microbiota diversity. As a food contaminant, the pesticide chlorpyrifos (CPF) has an effect on the composition of the intestinal microbiota and induces perturbation of microbiota. Prebiotics (and notably inulin) are known for their ability to promote an equilibrium of the microbiota that favours saccharolytic bacteria. The SHIME® dynamic in vitro model of the human intestine was exposed to CPF and inulin concomitantly for 30 days, in order to assess variations in both the bacterial populations and their metabolites. Various analyses of the microbiota (notably temporal temperature gradient gel electrophoresis) revealed a protective effect of the prebiotic through inhibition of the enterobacterial (E. coli) population. Bifidobacteria were only temporarily inhibited at D15 and recovered at D30. Although other potentially beneficial populations (lactobacilli) were not greatly modified, their activity and that of the saccharolytic bacteria in general were highlighted by an increase in levels of short-chain fatty acids and more specifically butyrate. Given the known role of host-microbiota communication, CPF's impact on the body's homeostasis remains to be determined.

Whether propylene oxide (PO) exposure is associated with hyperglycemia were rarely explored. We aimed to determine the relationship between PO exposure and glucose metabolism, and potential role of oxidative stress. Among 3294 Chinese urban adults, urinary PO metabolite (N-Acetyl-S-(2-hydroxypropyl)-L-cysteine, 2HPMA), biomarkers of oxidative DNA damage (8-oxo-7,8-dihydro-20-deoxyguanosine, 8-OHdG) and lipid peroxidation (8-isoprostane, 8-iso-PGF₂α) in urine were determined. The associations of 2HPMA with 8-OHdG, 8-iso-PGF₂α, fasting plasma glucose (FPG), and risk of diabetes were explored. The roles of 8-OHdG and 8-iso-PGF₂α on association of 2HPMA with FPG and risk of diabetes were detected. After adjusted for potential confounders, each 1-unit increase in log-transformed concentration of 2HPMA was associated with a 0.15-mmol/L increase in FPG level, and the adjusted OR (95% CI) of diabetes by the associations of log-transformed urinary 2HPMA concentrations was 1.47 (95% CI: 1.03–2.11). Combination effects of 2HPMA with 8-OHdG or 8-iso-PGF₂α on risk of diabetes were detected, and elevated 8-iso-PGF₂α significantly mediated 34.5% of the urinary 2HPMA-associated FPG elevation. PO exposure was positively associated with FPG levels and risk of diabetes. PO exposure combined with DNA oxidative damage or lipid peroxidation may increase the risk of diabetes, and lipid peroxidation may partially mediate the PO exposure-induced FPG elevation.

To decrease the concentration of the toxic metal cadmium (Cd) in topsoil, and the human food chain, many countries have limited the Cd concentration allowed in phosphorus (P) fertilisers. However, to inform those limits we need accurate estimates of Cd leaching from established farming systems. Different soil layers were sampled to 2000 mm depth of a long-term trial that had applied 22.5 kg P ha⁻¹ yr⁻¹ for 67 years to grazed pastures that received either no irrigation or were irrigated when soil moisture fell below 10 or 20%. The annual yield of Cd leaching from the top 150 mm of soil to the 151–250 mm layer was between 1.1 and 1.8 g ha⁻¹ with Cd leaching increasing with the frequency of irrigation. The rate of Cd accumulation measured to 2000 mm was within the mean and standard error estimated for treatments using a mass balance approach. Estimates of annual Cd leaching loss were like those established from field trials measuring leaching events over a year (0.3–1.8 g ha⁻¹) with a similar rate of P application (9–24 kg P ha⁻¹ yr⁻¹). Using a Cd leaching rate of 1.8 g ha⁻¹ yr⁻¹ and P applications rates of 22.5 kg P ha⁻¹, topsoil Cd concentrations may stop increasing if Cd concentrations in P fertiliser can be maintained at < 72 mg Cd kg⁻¹ P.

Contamination of urban surface waters by herbicides is an increasing concern; however, sources of contamination are poorly understood, hindering the development of mitigation and regulatory strategies. Impervious surfaces, such as concrete in driveways and paths are considered an important facilitator for herbicide runoff to urban surface waters following applications by residential homeowners. This study assessed the transferability of a herbicide from concrete pavers treated with an off-the-shelf product, containing simazine as the active herbicide, marketed for residential homeowner application to impervious surfaces. Commercially available pavers were treated according to label directions and the effects of exposure time prior to irrigation, repeated irrigations, and dry time between irrigations on transferability of simazine to runoff were assessed. Simazine transferability was greatest when receiving an initial irrigation 1 h after application, with concentrations in runoff reduced by half when exposure times prior to the first irrigation were >2 days. Concentrations remained stable for repeated irrigations up to 320 days and exposures to outdoor conditions of 180 days prior to a first irrigation. Dry time between irrigations significantly influenced simazine transfer to runoff. Dry periods of 140 days resulted in approximately a 4-times increase in simazine transferability to runoff. These results suggest that herbicides used by homeowners, or any other users, on impervious surfaces are available to contaminate runoff for prolonged time periods following application at concentrations that may pose risks to aquatic life and for reuse of harvested runoff on parks and gardens. Regulators should consider the potential of hard surfaces to act as reservoirs for herbicides when developing policies and labelling products.

Environmental pollution by metals has repercussions on wildlife health. It is known that some metals can have an influence on the neuroendocrine stress response, and at the same time, metals have pro-oxidant effects that can overwhelm the antioxidant system and cause oxidative stress. This study evaluates the association of metals with neuroendocrine stress activity and biomarkers of oxidative stress in 42 captive female Morelet's crocodiles (Crocodylus moreletii). We measured five metals of ecotoxicological importance (Hg, Cd, Pb, Cu and Zn), and three biomarkers of the oxidative stress response in the liver: glutathione (GSH) and glutathione disulfide (GSSG) as markers for antioxidant system and thiobarbituric acid reactive substances (TBARS) for oxidative damage. We also measured one biomarker of the neuroendocrine response to stress: corticosterone (B) in blood plasma. The mean ± SD concentrations of metals in the liver expressed in μg/g (dw) were: Cd: 0.004 ± 0.003, Hg: 0.014 ± 0.019, Cu: 0.017 ± 0.013, Zn: 0.043 ± 0.035, Pb: 0.16 ± 0.256. The mean ± SD of GSH was 0.42 ± 0.35 nmol/mg protein, the mean ± SD of GSSG was 0.24 ± 0.20 nmol/mg protein, the mean ± SD concentrations of TBARS were 0.36 ± 0.21 nmol/mg protein, and the mean ± SD of B was 393.57 ± 405.14 pg/mL. Hg presented a significant negative relationship with corticosterone. Cd had a negative relationship with both GSH and GSSG; meanwhile, Zn showed a negative relationship with TBARS levels, could be a protective element against hepatic oxidative damage. Finally, B had negative relationship with oxidative damage. The connection found between Hg and the neuroendocrine stress response, as well as the correlations of Cd and Zn with oxidative damage and antioxidant activity should be studied further, given their toxicological importance and implications for the conservation of C. moreletii and other crocodilians.

Halogenated PAHs (HPAHs) are ubiquitous in the environment and have a toxicity similar to that of dioxin. Microplastics exist widely in the environment, and their sorption allows them to act as carriers of HPAHs, potentially changing the bioavailability of HPAHs. However, to the best of our knowledge related studies are limited. In this study, degrading bacteria of five HPAHs were cultivated from mangrove sediments. Among them, the Hyphomicrobium genus has good degradation ability on 9−BrAnt, 2−BrPhe and 2−ClPhe. The degradation process is in line with the first−order degradation kinetic characteristics. The kinetic equations of five kinds of HPAHs showed that the degradation half−lives are 0.65 days (2−BrFle), 0.79 days (9−ClPhe), 1.50 days (2−ClAnt), 5.94 days (9−BrPhe) and 14.1 days (9−BrAnt). The greater the number of benzene rings and the heavier the halogen substituents, the slower the degradation of HPAHs. The sorption of microplastics inhibited the biodegradation of HPAHs, and the degradation half−life of HPAHs will be extended from 0.65 to 14.1 days (the average is 4.59 days) to 1.71–9.93 days (average 5.40 days) for PA, 0.70–35.2 days (average 12.8 days) for PE, 6.02–28.2 (average 15.7 days) days for POM, and 4.60–24.0 (average 19.2 days) days for PP, which is mainly related to the partition coefficient between microplastics and water. This study provides a reference for reducing the uncertainty of the ecological risk assessment of HOCs in the aquatic environment.

Source apportionment analyses are essential tools to determine sources of ambient coarse particles (2.5 <dₚ < 10 μm) and to disentangle their association and contribution from other pollutants, particularly PM₂.₅ (<2.5 μm). A semi-continuous sampling campaign was conducted using two virtual impactors/concentrators to enhance coarse particulate matter concentrations coupled with an online thermal-optical EC/OC monitor to quantify coarse PM–bound organic carbon volatility fractions (OC₁-OC₄) in central Los Angeles during the winter, spring, and summer of 2021. The total OC and its volatility fraction concentrations, meteorological parameters (i.e., wind speeds and relative humidity), vehicle miles traveled (VMT), and gaseous source tracers (i.e., O₃ and NO₂) were used as inputs to positive matrix factorization (PMF) model. A 3-factor solution identified vehicular emissions (accounting for 46% in the cold phase and 26% in the warm phase of total coarse OC concentrations), secondary organic carbon (27% and 37%), and re-suspended dust (27% and 37%) as the primary organic carbon sources of coarse PM. The re-suspended dust factor showed a higher contribution of more volatile organic carbons (i.e., OC₁ up to 77%) due to their re-distribution on dust particles, whereas the SOA factor was the dominant contributor to less volatile organic aerosols (i.e., OC₄ up to 54%), which are the product of reactions at high relative humidity (RH). Our findings revealed that the total OC concentrations in the coarse size range were comparable with those of previous studies in the area, underscoring the challenges in curtailing coarse PM-bound OC sources and the necessity of developing effective emission control regulations on coarse PM. The results from the current study provide insights into the seasonal and temporal variation of total OC and its volatility fractions in Los Angeles.

Sedimentary nitrogen (N) in lakes significantly influenced by eutrophication plays a detrimental role on the ecological sustainability of aquatic ecosystems. Here, we conducted a thorough analysis of the importance of N potentially released from sediments during the shift of “grass-algae” ecosystem in plateau lakes. From 1964 to 2013, the average total amount of sedimentary potential mineralizable organic nitrogen (PMON) and exchangeable N in whole Lake Dianchi were 5.50 × 10³ t and 3.44 × 10³ t, respectively. NH₄⁺-N was the main product (>90%) of sedimentary PMON mineralization. The PMON in sediments had great release potential, which tended to regulate the distribution of aquatic plants and phytoplankton in Lake Dianchi and facilitated the replacement of dominant populations. Moreover, NH₄⁺-N produced by sedimentary PMON mineralization and exchangeable NH₄⁺-N have increased the difficulty and complexity of ecological restoration in Lake Dianchi to a certain extent. This study highlights the importance of sedimentary N in lake ecosystem degradation, showing the urgent need to reduce the continuous eutrophication of lakes and restore the water ecology.

Light at night (LAN) has received increasing attention for its potential health hazards to human and animals. However, to our knowledge, no study has explored the specific effects of bedroom nighttime light exposure on allostatic load (AL). To investigate the association between bedroom individual-level LAN exposure and AL among young adults, an integrative index manifests multiple system dysregulation. Using data from a cohort of 484 Chinese young adults aged 16–22 years. Bedroom light was objectively recorded at 1-min intervals for two nights using a portable illuminance meter. Fasting blood samples were collected at one-year follow-up for the detection of AL parameters. AL score was derived as sum of the top quartile of twelve physiological biomarkers in four systems: metabolic system (BMI, WC, TC, HDL, LDL, TG, HbA1c, INS, GLU); cardiovascular system (SBP, DBP); immune and inflammatory systems (hs-CRP), with HDL was lowest quartile. Univariate and multivariate linear regression models were used to evaluate the association between LAN intensity with AL score and separate AL parameters. The average age of subjects was 18.7 years, 64.3% were female. The mean AL score of LAN group (average LAN intensity ≥ 3lx) was significantly higher than Dim group (3.6 ± 2.6 vs. 2.7 ± 2.1; P = 0.007). For each 1 lx increase of LAN intensity was associated with 0.15-unit increase in AL score (95% CI: 0.06, 0.24; P = 0.001). Moreover, LAN group was associated with increased 1.01-unit in AL score (95% CI: 0.36–1.66; P = 0.003) compared to Dim group. Significant associations between bedroom LAN exposure with allostatic load and separate AL biomarkers were observed in our study. Keeping bedroom darkness at night may be a practicable option to reduce the wear of multiple body systems and improve human cardiometabolic health from early in life.

Lichens serve as important bioindicators of air pollution in cities. Here, we studied the diversity of epiphytic lichens in the urban area of Munich, Bavaria, southern Germany, to determine which factors influence species composition and diversity. Lichen diversity was quantified in altogether 18 plots and within each, five deciduous trees were investigated belonging to on average three tree species (range 1–5). Of the 18 plots, two were sampled in control areas in remote areas of southern Germany. For each lichen species, frequency of occurrence was determined in 10 quadrats of 100 cm² on the tree trunk. Moreover, the cover percentage of bryophytes was determined and used as a variable to represent potential biotic competition. We related our diversity data (species richness, Shannon index, evenness, abundance) to various environmental variables including tree traits, i.e. bark pH levels and species affiliation and air pollution data, i.e. NO₂ and SO₂ concentrations measured in the study plots. The SO₂ levels measured in our study were generally very low, while NO₂ levels were rather high in some plots. We found that the species composition of the epiphytic lichen communities was driven mainly by NO₂ pollution levels and all of the most common species in our study were nitrophilous lichens. Low NO₂ but high SO₂ values were associated with high lichen evenness. Tree-level lichen diversity and abundance were mainly determined by tree traits, not air pollution. These results confirm that ongoing NO₂ air pollution within cities is a major threat to lichen diversity, with non-nitrophilous lichens likely experiencing the greatest risk of local extinctions in urban areas in the future. Our study moreover highlights the importance of large urban green spaces for species diversity. City planners need to include large green spaces when designing urban areas, both to improve biodiversity and to promote human health and wellbeing.