Environmental colloids play crucial roles in the transport of environmental pollutants in porous media by acting as pollutant carriers. In this work, the dispersion stability and correlated transport of kaolinite colloid were investigated as a function of solution pH, solution ionic strength, and concentration of humic acid (HA), the roles of kaolinite colloid in driving Eu(III) transport were discussed. The results showed that the dispersion of kaolinite colloid was favorable at alkaline and extremely acidic pH values, the trend of aggregation with varying pH was critically reversed at pH ∼3.2 due to the transformation of surface electrical properties. Cations with higher valence and mineral affinity showed a more significant contribution in inducing colloid aggregation, which was generally in accordance with the Schulze-Hardy rule and Hofmeister series. HA greatly increased the colloid stability by altering the surface electrostatic potential and steric effect. The Derjguin-Landau-Verwey-Overbeek (DLVO) model suggested that the electrostatic force between colloidal particles controlled the aggregation and destabilizing trend of colloid, and the theoretically calculated critical coagulation concentration was consistent with that determined from kinetic aggregation experiments. The roles of kaolinite colloid in driving Eu(III) transport varied under different conditions, and the transport behavior was highly correlated with the dispersion stability trend of colloid. These results can provide an enhanced understanding of the environmental fate of kaolinite colloid as well as commensal pollutants.

The reduction of nitrogen oxide (DeNOx) from flue gas by microalgae is a promising technology that has attracted increasing attention. Because the water source is a major limitation of microalgae application in the DeNOx from flue gas, we investigated the feasibility of using domestic wastewater (WW) as a water source. As a result, a biomass accumulation rate of 0.27 ± 0.01 mg L⁻¹ d⁻¹ was achieved by Tetradesmusobliquus PF3 cultivated in WW for 8 d, and 30 mg L⁻¹ of nitrate nitrogen was added to the WW to fulfill the nutrient requirements of the microalgae cells. The ammonium (NH₄⁺) nitrogen present in WW exerted inhibitory effects on the removal of nitric oxide (NO), thereby leading to 8% decrease removal efficiency in comparison with that using clean water and nutrients (BG11 medium). However, these inhibitory effects disappeared following the exhaustion of NH₄⁺ by T. obliquus PF3 after 1 d. To overcome the inhibition of NH₄⁺ and to achieve a high NO removal efficiency, a strategy of connecting two reactors in series was presented. The removal efficiency of NO by the two series reactors reached up to 71.2 ± 2.9%, which was significantly higher than that obtained by a single reactor (43.1 ± 3.6%). In addition, 70.9 ± 4.8% of the supplied NO was fixed into microalgae cells in the two reactors, which was 1.75 times higher than that in the single reactor (40.6 ± 5.1%), thereby suggesting that connecting two reactors in series rendered effective recovery of NO from flue gas using WW as a water source. In this study, we provided an economically viable water source for the application of microalgae in the biological DeNOx from flue gases.

Quantitating the health effects of employment history in factories, especially polluting ones, is essential for understanding the benefits or losses of industrialization in rural areas. Using a traced subset of nationwide panel data from 2005 covering five provinces, 101 villages, and 2026 households (collected recently in 2016) and the econometric models, this study estimated the effect of factory employment history on workers' health. The results showed that: the absolute number of factory workers increased from 1998 to 2015, and the proportion of factory workers was 7.68% in 2015; the absolute number and the proportion of farmers decreased from 63.84% in 1998 to 29.06% in 2015. Given that all the respondents live in rural areas, the HlthPlace (the first place the individual went to for their last illness in 2015) was selected as the main dependent variable of interest, and Hlthexp (Healthcare expenditure per person at last illness in 2015) and self-reported health were used as auxiliary dependent variables. The findings revealed that, after controlling the characteristics of individual, household, hospital and area, a one year increase of factory employment history corresponded to a 0.035 level increase in the probability of people choosing high-level hospital (p < 0.01) and a 237.61 yuan increase in healthcare expenditure (p < 0.1). The results also showed the adverse effect of self-reported health on factory employment history (p < 0.01). In addition, the relationship between the farming history and health was evaluated, and the econometric results showed that compared with factory employment history, farming history had opposite impacts on health (p < 0.01). Finally, the robustness check showed that the empirical results were reliable and that the initial results were robust. Generally, this study revealed the effect of overall factory employment on health, which is a useful research supplement to the studies on the health effects of specific pollution exposure.

Plastic pollution, which is one of the most important environmental problems at the present time, has been understood recently, and the effects of this pollution on ecosystem and biota are becoming a growing problem, especially in the aquatic ecosystems. Direct or indirect exposure to those particles leads to adverse effects on marine organisms. In the marine environment, plastic materials interact with other pollutants such as metals, thereby affecting the uptake levels of those pollutants in marine organisms. In the present study, the Manila clam Ruditapes philippinarum was exposed to polyethylene microbeads and mercury chloride in single, combined and incubated form at environmentally relative concentrations for one week in controlled laboratory conditions. The uptake and tissue distribution of both stressors as well as the vector role of microplastics on mercury uptake in the organisms were investigated. Filtration rates, biomarkers for immunomodulation and oxidative stress, and histological alterations were also evaluated. Microplastics were ingested by the clams, and translocated to the various tissues. However, contaminated microplastics displayed a negligible vector role in terms of mercury bioaccumulation in the clams. The single and interactive exposure of the stressors reduced the filtration rate in the clams. Both pollutants affected the immune system of the organisms. Histological alterations were determined in the gill and digestive gland tissues of the clams among the treatment groups, although oxidative stress biomarkers remained unchanged. This study suggests that the vector role of polyethylene microplastics in mercury uptake is negligible and reveals that the single and interactive one-week exposure of two pollutants induce toxicity in the manila clams.

Natural halloysite nanotubes (HNTs) with a hollow lumen are already applied in numerous fields and enter the environment in increasing quantities, which may have effects on animal and human health. However their in vivo toxicity in mammals is still largely unclear. The aim of this study is to assess acute oral toxicity of HNTs in the stomach of mice and recovery. Oral HNTs at low dose (5 mg HNTs/kg BW) for 30 days increased in daily food and water intake and promoted mouse growth with no obvious adverse effect on the stomach. The promotive effect on mouse growth disappeared after cessation of oral administration of the nanotubes. Oral HNTs for 30 days at high dose (50 mg HNTs/kg BW) induced Si and Al accumulation in the stomach, which caused oxidative stress, inflammation and iNOS-mediated damage in the organ. The damage in the stomach led to slight atrophic gastritis and reduced mouse growth. Oral HNTs-induced changes at high dose were not observed after a 30-days recovery period. The findings provided the evidence that oral HNTs-induced acute toxicity in the stomach was reversible. More importantly, this research showed that Al and Si were cleared out of the mice by hepatic excretion and renal excretion, respectively, during the recovery period. The results suggest that HNTs at low concentration in environments have no adverse effect on mice, while there are health risks to mice under severe contamination by HNTs.

Whether toxicity of silver nanoparticles (AgNPs) to organisms originates from the nanoparticles themselves or from the dissolved Ag-ions is still debated, with the majority of studies claiming that extracellular release of Ag-ions is the main cause of toxicity. The objective of this study was to determine the contributions of both particles and dissolved ions to toxic responses, and to better understand the underlying mechanisms of toxicity. In addition, the pathways of AgNPs exposure to plants might play an important role and therefore are explicitly studied as well. We systematically assessed the phytotoxicity, internalization, biodistribution, and antioxidant responses in lettuce (Lactuca sativa) following root or foliar exposure to AgNPs and ionic Ag at various concentrations. For each endpoint the relative contribution of the particle-specific versus the ionic form was quantified. The results reveal particle-specific toxicity and uptake of AgNPs in lettuce as the relative contribution of particulate Ag accounted for more than 65% to the overall toxicity and the Ag accumulation in whole plant tissues. In addition, particle toxicity is shown to originate from the accumulation of Ag in plants by blocking nutrient transport, while ion toxicity is likely due to the induction of excess ROS production. Root exposure induced higher toxicity than foliar exposure at comparable exposure levels. Ag was found to be taken up and subsequently translocated from the exposed parts of plants to other portions regardless of the exposure pathway. These findings suggest particle related toxicity, and demonstrate that the accumulation and translocation of silver nanoparticles need to be considered in assessment of environmental risks and of food safety following consumption of plants exposed to AgNPs by humans.

In the general population, chronic exposure to low-dose persistent organic pollutants (POPs), particularly organochlorine pesticides (OCPs), has been recently linked to many chronic diseases. Widespread contamination of the food chain and human adipose tissue has made avoiding exposure to these chemicals impossible; thus, alternative strategies for decreasing the chemical burden must be investigated. Recently, macronutrient intake was found to significantly modify the toxicokinetics of POPs in animal experimental studies. Thus, we evaluated whether macronutrient intake was related to serum concentrations of OCPs in healthy adults without cardio-metabolic diseases. Subjects included 1,764 adults, aged 20 years or above, who participated in the National Health and Nutrition Examination Survey 1999–2004. Macronutrient intake was assessed based on a 24-h dietary recall interview. Six individual OCPs commonly detected among the general population were evaluated as markers of OCPs and other coexisting lipophilic chemicals stored in adipose tissue and released into circulation. High fat intake was associated with lower concentrations of OCPs, while high carbohydrate intake showed the opposite result. When three types of fats were individually evaluated, both saturated fatty acids and monounsaturated fatty acids, but not polyunsaturated fatty acids, were inversely associated with serum concentrations of OCPs. Adjustment for possible confounders did not change the results. When stratified by age, gender, body mass index, and physical activity, these associations were similar in most subgroups. Thus, similar to the findings observed in animal experimental studies, a moderate-fat diet with low carbohydrate intake was related to low serum concentrations of OCPs in humans. Although these findings need to be replicated, changing dietary macronutrient intake can be investigated as a practical strategy for dealing with unavoidable lipophilic chemical mixtures such as OCPs in modern society.

Thallium (Tl) is a dispersed trace metal showing remarkable toxicity. Various anthropogenic activities may generate Tl contamination in river sediments, posing tremendous risks to aquatic life and human health. This paper aimed to provide insight into the vertical distribution, risk assessment and source tracing of Tl and other potentially toxic elements (PTEs) (lead, cadmium, zinc and copper) in three representative sediment cores from a riverine catchment impacted by multiple anthropogenic activities (such as steel-making and Pb–Zn smelting). The results showed high accumulations of Tl combined with associated PTEs in the depth profiles. Calculations according to three risk assessment methods by enrichment factor (EF), geoaccumulation index (Igₑₒ) and the potential ecological risk index (PERI) all indicated a significant contamination by Tl in all the sediments. Furthermore, lead isotopes were analyzed to fingerprint the contamination sources and to calculate their quantitative contributions to the sediments using the IsoSource software. The results indicated that a steel-making plant was the most important contamination source (∼56%), followed by a Pb–Zn smelter (∼20%). The natural parental bedrock was found to contribute ∼24%. The findings highlight the importance of including multiple anthropogenic sources for quantitative fingerprinting of Tl and related metals by the lead isotopic approach in complicated environmental systems.

A chemical contaminant of growing concern to freshwater aquatic organisms, including many amphibians, is chloride ion. The salinization of freshwater ecosystems is likely caused, in part, by the application of massive amounts of road de-icing salts to roadways during winter months. The issue of freshwater salinization has become the subject of many toxicity studies and is often investigated in conjunction with other chemical stressors. However, few published studies attempt to investigate the interactions of elevated chloride concentration and increased temperature. Further, no studies have investigated the gap between the recommended feeding conditions typically used in standard toxicity tests and those that may exist in natural amphibian habitats. This study addressed the critical issues of elevated chloride, increased temperature, and variation in food quality. We conducted a 96-h acute toxicity test to investigate acute chloride toxicity as impacted by different diets, as well as a chronic toxicity test to investigate the impacts of chloride, temperature, and resource quality on the survival and development of larval Lithobates sylvaticus (wood frogs). Chloride LC₅₀s ± 1 SE were 3769.22 ± 589.05, 2133.00 ± 185.95, and 2644.69 ± 209.73 mg Cl⁻/L were for non-fed, low-protein diet, and high-protein diet, respectively. For the chronic toxicity study, elevated chloride decreased tadpole survival. Increased temperature, and lower resource quality, were found negatively impacted survival of tadpoles and altered time-to-metamorphosis. This study shows that environmentally relevant concentrations of chloride, temperatures, and the protein content of the diet all exert critical effects on larval wood frogs.

Freshwater planarians have been gaining relevance as experimental animals for numerous research areas given their interesting features, such as high regeneration potential, shared features with the vertebrates’ nervous system or the range of endpoints that can be easily evaluated in response to contaminants. Ecotoxicological research using these animals has been steadily increasing in the past decades, as planarians’ potentialities for this research area are being recognized. In this work, we used polycyclic aromatic hydrocarbons (PAHs) as model contaminants and evaluated effects of exposure to phenanthrene, pyrene and benzo[a]pyrene (B[a]P) in planarians. The freshwater planarian Girardia tigrina was chosen and mortality, cephalic regeneration (during and post-exposure), behavioral endpoints and presence of PAHs in tissues, were evaluated. Mortality was only observed in planarians exposed to phenanthrene, with an estimated LC₅₀ of 830 μg L⁻¹. Results indicate that planarian behavioral endpoints were very sensitive in response to sub-lethal concentrations of PAHs, showing a greater sensitivity towards B[a]P and pyrene. Briefly, post-exposure locomotion and post-exposure feeding were significantly impaired by sub-lethal concentrations of all compounds, whereas regeneration of photoreceptors was only significantly delayed in planarians exposed to pyrene. Moreover, levels of PAH-type compounds in planarian tissues followed a concentration-dependent increase, showing uptake of compounds from experimental solutions. The present results highlight the importance of studying alternative and complementary endpoints, such as behavior, not only because these may be able to detect effects at lower levels of contamination, but also due to their ecological relevance. The simplicity of evaluating a wide range of responses to contaminants further demonstrates the utility of freshwater planarians for ecotoxicological research.