The effects of elevated carbon dioxide (CO₂) and nitrogen (N) addition on foliar N and phosphorus (P) stoichiometry were investigated in five native tree species (four non-N₂ fixers and one N₂ fixer) in open-top chambers in southern China from 2005 to 2009. The high foliar N:P ratios induced by high foliar N and low foliar P indicate that plants may be more limited by P than by N. The changes in foliar N:P ratios were largely determined by P dynamics rather than N under both elevated CO₂ and N addition. Foliar N:P ratios in the non-N₂ fixers showed some negative responses to elevated CO₂, while N addition reduced foliar N:P ratios in the N₂ fixer. The results suggest that N addition would facilitate the N₂ fixer rather than the non-N₂ fixers to regulate the stoichiometric balance under elevated CO₂.

High spatial heterogeneity of physical and chemical sediment properties was observed in both horizontal and vertical directions of deposits in a groyne field of the Middle Elbe. The respective sediment cores were less polluted on the top compared to consolidated deeper layers, indicating a decreasing trend of contamination in the river basin. In contrast to water quality monitoring, the impact of the large Elbe flood in 2002 was still visible in the deeper layers (5–30 cm) of the groyne field sediments six years after the event. Due to the fact that the environmental risk increases with erosion potential of discharge and contamination level of sediments, monitoring for environmental risk must capture not only surface sediments but also deeper layers up to an anticipated erosion depth.

Atmospheric deposition of Hg is the predominant pathway for Hg to reach sensitive ecosystems, but the importance of emissions on near-field deposition remains unclear. To better understand spatial variability in Hg deposition, mercury concentrations were analyzed in sediment cores from 12 lakes with undeveloped watersheds near to (<50 km) and remote from (>150 km) several major urban areas in the United States. Background and focusing corrected Hg fluxes and flux ratios (modern to background) in the near-urban lakes (68 ± 6.9 μg m⁻² yr⁻¹ and 9.8 ± 4.8, respectively) greatly exceed those in the remote lakes (14 ± 9.3 μg m⁻² yr⁻¹ and 3.5 ± 1.0) and the fluxes are strongly related to distance from the nearest major urban area (r² = 0.87) and to population and Hg emissions within 50–100 km of the lakes. Comparison to monitored wet deposition suggests that dry deposition is a major contributor of Hg to lakes near major urban areas.

The global distribution and long-range transport of polyfluoroalkyl substances (PFASs) were investigated using seawater samples collected from the Greenland Sea, East Atlantic Ocean and the Southern Ocean in 2009–2010. Elevated levels of ΣPFASs were detected in the North Atlantic Ocean with the concentrations ranging from 130 to 650 pg/L. In the Greenland Sea, the ΣPFASs concentrations ranged from 45 to 280 pg/L, and five most frequently detected compounds were perfluorooctanoic acid (PFOA), perfluorohexanesulfonate (PFHxS), perfluorohexanoic acid (PFHxA), perfluorooctane sulfonate (PFOS) and perfluorobutane sulfonate (PFBS). PFOA (15 pg/L) and PFOS (25–45 pg/L) were occasionally found in the Southern Ocean. In the Atlantic Ocean, the ΣPFASs concentration decreased from 2007 to 2010. The elevated PFOA level that resulted from melting snow and ice in Greenland Sea implies that the Arctic may have been driven by climate change and turned to be a source of PFASs for the marine ecosystem.

Polybrominated diphenyl ethers (PBDEs) were determined in sewage sludge samples collected from eight Italian wastewater treatment plants (WWTPs) between June 2009 and March 2010. Total PBDE concentrations ranged from 158.3 to 9427 ng g⁻¹ dw, while deca-BDE (BDE-209) (concentrations ranging from 130.6 to 9411 ng g⁻¹ dw) dominated the congener profile in all the samples, contributing between 77% and 99.8% of total PBDE. The suitability of using a magnetic particle enzyme-linked immunoassay (ELISA) to analyse PBDEs in sewage sludge was also tested. The ELISA results, expressed as BDE-47 equivalents, were well correlated with those obtained by GC–NCI–MS, with correlation coefficients (r²) of 0.899 and 0.959, depending on the extraction procedure adopted. The risk assessment of PBDEs in sewage sludge addressed to land application was calculated. PECₛₒᵢₗ values compared to the relative PNECₛₒᵢₗ for penta and deca-BDE suggests that there is a low risk to the soil environment.

The discharge of wastewater from sewage treatment plants is one of the most common forms of pollution to river ecosystems, yet the effects on aquatic invertebrate assemblages have not been investigated in a controlled experimental setting. Here, we use a mesocosm approach to evaluate community responses to exposure to different concentrations of treated wastewater effluents over a two week period. Multivariate analysis using Principal Response Curves indicated a clear, dose-effect response to the treatments, with significant changes in macroinvertebrate assemblages after one week when exposed to 30% effluent, and after two weeks in the 15% and 30% effluent treatments. Treatments were associated with an increase in nutrient concentrations (ammonium, sulfate, and phosphate) and reduction of dissolved oxygen. These findings indicate that exposure to wastewater effluent cause significant changes in abundance and composition of macroinvertebrate taxa and that effluent concentration as low as 5% can have detectable ecological effects.

The effects of Pb pollution on cattle and sheep raised in an ancient mining area were studied through the use of blood Pb (PbB) levels and δ-aminolevulinic acid dehydratase (δ-ALAD) activity. Lead levels in livestock blood from the mining area (n=110) were significantly elevated when compared to the controls (n=79). In 91.4% of cattle (n=58) and 13.5% of sheep (n=52) sampled in the mining area, PbB levels corresponded to subclinical exposure (6–35μg/dl). Two young cattle (<2 years) from the mining area (n=5) had PbB levels indicative of clinical poisoning (>35μg/dl). Elevated PbB was also accompanied by δ-ALAD activity inhibition in blood, which confirms that measurable effects of Pb poisoning were taking place. Observed PbB levels suggest that a potential risk to human consumers of beef from the Pb polluted areas may also exist, as has been shown previously for game meat from the same mining area.

Oxidative damage has been proposed as an important mechanism linking pesticide exposure to health effects. A study of 268 young Shanghai children was conducted to examine the relationship between organophosphate pesticide (OP) exposure and a biomarker of oxidative DNA damage. Urine samples were analyzed for five nonspecific dialkyl phosphate (DAP) metabolites [dimethyl phosphates (DMs) and diethyl phosphates (DEs)] and 8-hydroxy-2′-deoxyguanosine (8-OHdG). The creatinine-adjusted median of 8-OHdG in urine samples was 3.99ng/mg. Increased exposure to OPs was associated with greater levels of urinary 8-OHdG [total DAPs: ß (adjusted)=0.46 per log₁₀ unit increase, 95% confidence interval (CI)=0.40–0.53, p=0.000; DMs: ß (adjusted)=0.34, 95% CI=0.28–0.41, p=0.000; DEs: ß (adjusted)=0.48, 95% CI=0.42–0.54, p=0.000]. Thus, the 8-OHdG biomarker is useful for increasing our understanding of the link between childhood exposure to OPs and health outcomes.