A manipulative field experiment was designed to investigate the effects of sediment-nutrients and sediment-organic matters on seagrasses, Zostera japonica, using individual and population indicators. The results showed that seagrasses quickly responded to sediment-nutrient and organic matter loading. That is, sediment-nutrients positively impacted on seagrasses by increasing N content of leaves and roots, leaf length and belowground biomass. Sediment-organic matter loading lowered N content of seagrass leaves and belowground biomass. Negative effects of organic matter loading were aggravated during nutrient loading, by decreasing N content of leaves, P content of roots, leaf width, shoot number in the middle period of the experiment, increasing C/N ratio of leaves, C/P and N/P ratio of roots and above to belowground biomass ratio of seagrasses. Consequently, Z. japonica could be considered as a fast indicator to monitor seagrass ecosystem status in the eutrophic areas and facilitate to interpreting the response of seagrasses to multiple stressors.

Traffic-related air pollutant emissions have become a global environmental problem, especially in urban areas. The estimation of pollutant emissions is based on complex models that require the use of detailed travel-activity data, which is often unavailable and in particular, in developing countries. In order to overcome this issue, an alternative multiple-input–multiple-output general regression neural network model, based on basic socioeconomic and transport related indicators, is proposed for the simultaneous prediction of sulphur oxides (SOx), nitrogen oxides (NOx), ammonia (NH3), non-methane volatile organic compounds (NMVOC) and particulate matter emissions at the national level. The best model, created using only six inputs, has MAPE (mean absolute percentage error) values on testing in the range of 12–15% for all studied pollutants, except NMVOC (MAPE = 21%). The obtained predictions for SOx, NH3 and PM10 emissions were in good agreement with the reported emissions (R2 ≥ 0.93), while the predictions for NOx and NMVOC are somewhat less accurate (R2 ≈ 0.85). It can be concluded that the presented ANN approach can offer a simple and relatively accurate alternative method for the estimation of traffic-related air pollutant emissions.

The aim of this study is to develop standard statistical methods and software for the analysis of beach litter data. The optimal ensemble of statistical methods comprises the Mann-Kendall trend test, the Theil-Sen slope estimation, the Wilcoxon step trend test and basic descriptive statistics. The application of Litter Analyst, a tailor-made software for analysing the results of beach litter surveys, to OSPAR beach litter data from seven beaches bordering on the south-eastern North Sea, revealed 23 significant trends in the abundances of beach litter types for the period 2009–2014. Litter Analyst revealed a large variation in the abundance of litter types between beaches. To reduce the effects of spatial variation, trend analysis of beach litter data can most effectively be performed at the beach or national level. Spatial aggregation of beach litter data within a region is possible, but resulted in a considerable reduction in the number of significant trends.

Anthropogenic noise is a significant pollutant of the world's oceans, affecting behavioural and physiological traits in a range of species, including anti-predator behaviours. Using the open field test, we investigated the effects of recordings of piling and drilling noise on the anti-predator behaviour of captive juvenile European seabass in response to a visual stimulus (a predatory mimic). The impulsive nature of piling noise triggered a reflexive startle response, which contrasted the behaviour elicited by the continuous drilling noise. When presented with the predatory mimic, fish exposed to both piling and drilling noise explored the experimental arena more extensively than control fish exposed to ambient noise. Fish under drilling and piling conditions also exhibited reduced predator inspection behaviour. Piling and drilling noise induced stress as measured by ventilation rate. This study provides further evidence that the behaviour and physiology of European seabass is significantly affected by exposure to elevated noise levels.

Aliphatic and polycyclic aromatic hydrocarbons (PAHs) were quantified in harbors sediments from SE Brazil. The aims were to (i) compare harbors with distinct sizes and under the influence of different environmental pressures with respect to the sources and level of hydrocarbon contamination and (ii) evaluate the potential of adverse biological effects of sediment contamination by PAHs, based on sediment quality guidelines and toxicity equivalence quotient. The concentrations of total aliphatics (39.9μgg−1; 22.9–113μgg−1) and total PAHs (727ngg−1; 366–1760ngg−1) varied according to the size of and urban development around each harbor. Mixed contamination by petrogenic and pyrolytic hydrocarbons, and input of biogenic compounds, indicated the influence of both harbor activities and a myriad of urban, industrial and atmospheric inputs. The quality of sediment poses a risk to benthic biological communities, and, if dredged, this material may cause environmental damage in the waste disposal area.

A 3-year research program was undertaken to assess potential environmental disturbance caused by the Deepwater Horizon oil spill to the soft-bottom macrobenthic communities within Mexican waters of the northwestern Gulf of Mexico. Community properties and temporal/spatial variability were analyzed besides toxicant parameters such as hydrocarbons and trace-metals. Overall infaunal density increased, taxa proportion changed, and small-size opportunistic organisms prevailed throughout the study. Annual abundance-biomass comparison (ABC) curves revealed progressive stress scenarios from moderate to severe. Concentrations of vanadium, nickel, cobalt, PAHs and AHs increased gradually over time. However, low correlations between benthic density and biogeochemical variables were determined. Initially, sedimentary properties were the main drivers of benthic community structure; subsequently, nickel, vanadium and PAHs, indicative of anthropogenic effect, were highlighted. Interannual variability in the macroinfauna was attributed to the synergy of several environmental factors. Undoubtedly, compounds derived from fossil fuels had a significant disturbance role, but their source remains uncertain.

Panamá's extreme hydroclimate seasonality is driven by Intertropical Convergence Zone rainfall and resulting runoff. River discharge (Q) carries terrestrially-derived barium to coastal waters that can be recorded in coral. We present a Ba/Ca record (1996–1917) generated from a Porites coral colony in the Gulf of Chiriquí near Coiba Island (Panamá) to understand regional hydroclimate. Here coral Ba/Ca is correlated to instrumental Q (R=0.67, p<0.001), producing a seasonally-resolved Reduced Major Axis regression of Ba/Ca (μmol/mol)=Q (m3/s)×0.006±0.001 (μmol/mol)(m3/s)−1+4.579±0.151. Our results support work in the neighboring Gulf of Panamá that determined seawater Ba/Ca, controlled by Q, is correlated to coral Ba/Ca (LaVigne et al., 2016). Additionally, the Coiba coral Ba/Ca records at least 5 El Niño events and identified 22 of the 37 wet seasons with below average precipitation. These data corroborate the Q proxy and provide insight into the use of coral Ba/Ca as an El Niño and drought indicator.

Ballast water is taken on-board vessels into ballast water tanks to maintain vessel draft, buoyancy, and stability. Unmanaged ballast water contains aquatic organisms that, when transported and discharged to non-native waters, may establish as invasive species. Technologies capable of achieving regulatory limits designed to decrease the likelihood of invasion include onboard ballast water management systems. However, to date, the treatment development and manufacturing marketplace is limited to large vessels with substantial ballast requirements. For smaller vessels or vessels with reduced ballast requirements, we evaluated the feasibility of meeting the discharge limits by generating ballast water using onboard potable water generators. Case studies and parametric analyses demonstrated the architectural feasibility of installing potable water generators onboard actual vessels with minimal impacts for most vessel types evaluated. Furthermore, land-based testing of a potable water generator demonstrated capability to meet current numeric discharge limits for living organisms in all size classes.

This study aimed to determine the role of mangroves as a biofilter of heavy metals. The concentrations of heavy metals, namely copper, mercury, cadmium, zinc, and lead, in the mangroves Rhizophora apiculata, Ceriops tagal, Bruguiera gymnorrhiza, Lumnitzera racemosa, Xylocarpus granatum, Sonneratia alba, and Bruguiera parviflora at RAWN Park were determined using a Flame atomic absorption spectrophotometer. High concentrations of Cu (83.85μgg−1) and Hg (0.52μgg−1) were found in the tissues of L. racemosa, while high concentrations of Cd (10.81μgg−1), Zn (70.41μgg−1), and Pb (1.36μgg−1) were found in the tissues of B. gymnorrhiza, B. parviflora and C. tagal, respectively. The translocation and bioaccumulation factors of heavy metals by mangroves showed a variety of trends, which indicated the different partitioning and uptake capability of heavy metals in the tissues of various mangrove species. Thus, maintaining high diversity of mangroves is crucial to ensure the health and productivity of coastal zones.

Comparative studies were developed with regard to the criteria emissions of THC - total hydrocarbons, CO - carbon monoxide and NOx - nitrogen oxides, and BTEX (benzene, toluene, ethyl benzene and xylenes). Three four-stroke motorcycles were fuelled with E22 fuel (gasoline with 22% of ethanol). One flex fuel motorcycle was also fuelled with E100, 100% Hydrated Reference Ethanol, and with E61 (61% of ethanol). Criteria emissions were quantified using traditional measurement techniques based on the European Directive 97/24/EC (EURO protocol). To determine the BTEX emissions, gas chromatography coupled with mass spectrometry was employed. All motorcycles were equipped with a catalyst (TWC) used to reduce the amount of toxic emissions in the exhaust gases. To evaluate the performance of the catalyst, one motorcycle was tested with and without the device. One motorcycle was tested according to two different test protocols, namely, the ECE/TRANS/180 WMTC, also known as the Worldwide Motorcycle Test Cycle, and the EURO protocol. The main results were that toluene emissions were more prevalent than other aromatics. The tests with and without the catalyst showed that after the catalyst was employed the conversion efficiencies for benzene, toluene, ethyl benzene and xylene were 52.3%, 84.0%, 85.0%, and 86.0%, respectively. The results regarding the flex fuel motorcycle show that BTEX emissions decrease with an increase of ethanol in the mixture. Comparing the EURO and WMTC protocols, it was observed that the WMTC protocol generates lower emissions compared to the EURO protocol; however, NOx showed the opposite trend of BTEX.