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Результаты 2031-2040 из 4,308
Extracellular Polymeric Substances from a Fungus Are More Effective than Those from a Bacterium in Polycyclic Aromatic Hydrocarbon Biodegradation Полный текст
2017
Jia, Chunyun | Li, Xiaojun | Zhang, Lifang | Francis, Dennis | Tai, Peidong | Gong, Zongqiang | Liu, Wan
To investigate the differential contribution of extracellular polymeric substances (EPS) to polycyclic aromatic hydrocarbon (PAH) degradation by fungi and bacteria, the PAH-degrading ability and characteristics of EPS from the bacterium Mycobacterium gilvum SN12 and the fungus Mucor mucedo were compared. The fungus degraded 11% more pyrene and 21% more benzo[a]pyrene (B[a]P) than the bacterium. The biodegradation of pyrene and B[a]P increased after EPS were introduced into the PAH degradation solution, and 5.0% more pyrene and 4.5% more B[a]P were achieved for the added EPS from the fungal compared with the bacterial isolate. The comparison of two EPS indicated that the amount of protein and carbohydrate in EPS from the fungus was greater than that from the bacterium and was especially enriched for tryptophan, which is positively related to the increase of PAH biodegradation by fungal EPS. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) further revealed that higher molecular weight (HMW) of proteins over 200 kDa only existed in EPS from the fungus, and the polymorphism of proteins in EPS from the fungus was more abundant than that from the bacterium. The HMW proteins with stronger hydrophobicity in the fungal EPS also enabled the fungus to absorb more PAH than the bacterium. The results demonstrated that the pronounced differences in the characteristics of EPS from the fungal and the bacterial sources are responsible for the differential effects on PAH biodegradation.
Показать больше [+] Меньше [-]Effluents from Anaerobic Digestion of Organic Wastes: Treatment by Chemical and Electrochemical Processes Полный текст
2017
Fernandes, Annabel | Jesus, Tatiana | Silva, Rodrigo | Pacheco, MariaJosé | Ciríaco, Lurdes | Lopes, Ana
The treatment of effluent from anaerobic digestion of organic wastes was carried out using chemical and electrochemical processes, namely, chemical coagulation (CC) with lime, electrocoagulation (EC) with iron consumable electrodes, and electrochemical oxidation (EO) with a boron-doped diamond anode, at different experimental conditions. In the CC assays, the highest chemical oxygen demand (COD) removal, 50%, was achieved for a lime concentration of 70 g L⁻¹ after 2 h experiment. Under the experimental conditions studied, EC promoted COD removals of 80% after 5 h and EO led to COD removals of 43% after 6 h electrolysis, being this last removal increased to 60% when chloride was added to the effluent. A combined EC+EO treatment was also performed, utilizing the most favorable experimental conditions obtained in the individual processes, and global removals of 95% in COD and 44% in ammonia nitrogen were attained after 5 h of EC followed by 6 h of EO. These results proved that the combined process can be an efficient alternative in the treatment of effluents from anaerobic digestion of organic wastes with the characteristics of the studied effluent.
Показать больше [+] Меньше [-]Multitrophic Assessment of Copper Contamination Using Watercress (Nasturtium officinale) and the Mustard Beetle (Phaedon viridis) Полный текст
2017
Beals, Christopher | Byl, Thomas
Copper is an active component of some commercial algaecides and is commonly found in low concentrations in contaminated aquatic systems. Unintended consequences of algaecide application include macrophyte bioaccumulation and possible trophic level bioamplification especially by specialist herbivores. Trophic level effects of copper contamination were observed through feeding trials using the mustard beetle (Phaedon viridis). Several metals, including copper, interfere with the myrosinase enzyme system responsible for the watercress (Nasturtium officinale) allelopathic defense against herbivory. The mustard beetle is a specialist herbivore that has evolved a detection system that is stimulated by the products of the glucosinolate-myrosinase system. Because copper interferes with myrosinase enzymes, mustard beetles were expected to avoid copper-contaminated plants. While larvae exhibited a slight preference for contaminated plants, adult mustard beetles in this experiment exhibited a statistically significant preference for plants uncontaminated with copper.
Показать больше [+] Меньше [-]Development of Highly Selective and Efficient Prototype Sensor for Potential Application in Environmental Mercury Pollution Monitoring Полный текст
2017
Sarkar, Probir Kumar | Halder, Animesh | Polley, Nabarun | Pal, Samir Kumar
Mercury (Hg) is an environmental pollutant which is detrimental to the health of living beings due to the toxicity in its all oxidation states. To control mercury pollution development of low cost, efficient and highly sensitive prototype mercury sensor remains a challenge. In the present work, we have proposed a low-cost prototype device based on silver nanoparticle-impregnated poly(vinyle alcohol) (PVA-Ag-NPs) nanocomposite thin film for mercury detection. The thin film, fabricated through a facile protocol, is shown to be a fast, efficient, and selective sensor for Hg²⁺ in aqueous medium with a detection limit of 10 ppb. We have utilized the aggregation and amalgamation of Ag-NPs with Hg²⁺ to develop the low-cost, highly efficient and feasible prototype mercury sensor. In the presence of Hg²⁺, the yellowish thin film turned into colourless due to the loss of intense surface plasmon resonance (SPR) absorption band of the silver nanoparticles (Ag-NPs) through aggregation and amalgamation with mercury. The developed sensor has high selectivity for Hg²⁺ ions over a wide range of other competing heavy metal ions, generally present in water of natural sources. The sensor response is found to be linear over the Hg²⁺ ion concentration regime from 10 ppb to 5 ppm. The developed sensor has shown to determine a trace Hg²⁺ ions in real water samples. Finally, using the proposed technique, we have developed a simple and inexpensive prototype device for monitoring in field environmental mercury pollution. Graphical Abstract ᅟ
Показать больше [+] Меньше [-]Washout of Fine Sand Particles From a Ceramic Tile Roof: Laboratory Experiments Under Simulated Rainfall Полный текст
2017
Silveira, Alexandre | de Lima, JoãoL. M. P. | Abrantes, JoãoR. C. B. | Mujtaba, Babar
Roof runoff is an important source of urban stormwater and a main source of rainwater harvesting. Deposition of pollutants on rooftops can have a negative impact on runoff quality and, therefore, on harvested rainwater. Laboratory experiments with simulated rainfall were performed in order to study the washout of fine sand particles deposited on a ceramic tile roof, by runoff, considering the effect of the particle position, particle areal load, particle connectivity and roof slope. Results indicated that particle washout was influenced by the particle position on the roof; particle transport peak and transported mass was higher for the particle mass positions closer to the outlet. Increase in particle areal load decreased particle transport whereas particle connectivity had no effect on particle transport. However, roof slope was a dominant aspect in the particle washout; increase in roof slope greatly increased particle transport peak and transported mass. It also remarkably increased the first flush effect.
Показать больше [+] Меньше [-]Artificial Neural Network (ANN) for Modelling Adsorption of Lead (Pb (II)) from Aqueous Solution Полный текст
2017
Khan, Taimur | Mustafa, MuhammadRaza Ul | Isa, MohamedHasnain | Manan, TehSabariah Binti Abd | Ho, Yeek-Chia | Lim, Jun-Wei | Yusof, NurZulaikha
This study investigated the efficiency of rice husk carbon (RHC) for lead (Pb (II)) adsorption. The developed RHC was characterized by CHNS analyser, FTIR and FESEM. BET surface area, micropore area, micropore volume and average pore diameter of RHC were 58.54 m²/g, 14.53 m²/g, 0.007209 mL/g, and 45.46 Å, respectively. Batch adsorption experiments were conducted to assess the effect of initial pH, contact time, initial Pb (II) concentration and RHC dose on Pb (II) removal. A contact time of 120 min and a pH value of 5 were found as optimum for Pb (II) adsorption; maximum adsorption occurred at 8 g/L of RHC dose. Artificial neural network (ANN) was applied to model Pb (II) adsorption. For prediction of Pb (II) adsorption from aqueous solution by RHC, the smallest mean square error (MSE) and the largest coefficient of determination (R²) values were, respectively, obtained as 6.0053 and 0.988567 with Levenberg–Marquardt algorithm (LMA). Hence, it was selected as the best training algorithm. Traincgf and traincgp functions followed this function with a MSE of 6.1496 and 6.2967, respectively. Adsorption of Pb (II) by RHC followed pseudo-second-order kinetics. The experimental data were described well by both Langmuir and Freundlich isotherm models. Thermodynamics study revealed that Pb (II) adsorption by RHC was spontaneous and endothermic, and the system randomness increased during the whole process. Pb (II) adsorption capacity of RHC was compared with different adsorbents. As evidenced by its high adsorption capacity, RHC can be used as an effective adsorbent for Pb (II) removal from aqueous solutions and wastewaters.
Показать больше [+] Меньше [-]Effects of Chronic Exposure to Silver Nanoparticles on Ruditapes decussatus Gills Using Biochemical Markers Полный текст
2017
Hidouri, Slah | Ensibi, Chérif | Landoulsi, Ahmed | Daly-Yahia, Mohamed Néjib
Nanoparticles are among the particular materials produced by industrial activities; the release of these nanoparticles in natural ecosystems interacts with living organisms. Aquatic environment is the most common estuary waste medium for industrial and all human activities, the consequences may be highly effective on sea food species. Moreover, the potential in situ reduction of metallic ions by preexistent agents leads to nanoparticles which may cause hazardous effects. Many organisms become at risk especially those that use gills during respiration process such as bivalves. The study undertaken investigates the potential effect of silver nanoparticles obtained by green synthesis method on the gills of Ruditapes decussatus as a model. Nanoparticles have been synthesized using Ceratonia siliqua fruit extract as a reducing agent. The organisms have been chronically exposed to silver nanoparticles and the effects were biochemically evaluated. The tests performed show a typical behavior of catalase, glutathione reductase, and glutathione S-transferase activities that give information about the oxidative stress-induced malondialdehyde quantification, which reveals a possible membranous deterioration of the gills. Acetylcholinesterase expression has been qualified to be at a safe rate which implies the capacity of the animal to protect the cholinergic system.
Показать больше [+] Меньше [-]The Role of Compost in Stabilizing the Microbiological and Biochemical Properties of Zinc-Stressed Soil Полный текст
2017
Strachel, Rafał | Wyszkowska, Jadwiga | Baćmaga, Małgorzata
The progressive development of civilization and intensive industrialization has contributed to the global pollution of the natural environment by heavy metals, especially the soil. Degraded soils generally contain less organic matter, and thus, their homeostasis is more often disturbed, which in turn manifests in changes in biological and physicochemical properties of the soil. Therefore, new possibilities and solutions for possible neutralization of these contaminations are sought, inter alia, through reclamation of degraded land. At present, the use of additives supporting the reclamation process that exhibit heavy metal-sorbing properties is becoming increasingly important in soil recovery. Research was conducted to determine the role of compost in stabilizing the microbial and biochemical balance of the soil due to the significant problem of heavy metal-contaminated areas. The study was conducted on loamy sand, to which zinc was applied at the following doses: 0, 250, 500, 750, 1000, and 1250 mg Zn²⁺ kg⁻¹ DM of soil. Compost was introduced to the appropriate objects calculated on the basis of organic carbon content in the amount of 0, 10, and 20 g Cₒᵣg kg⁻¹ DM of soil. The study was conducted over a period of 20 weeks, maintaining soil moisture at 50% capillary water capacity. Zinc significantly modified soil microbiome status. The abundance of microorganisms and their biological diversity and the enzymatic activity of the soil were affected. The negative effects of contaminating zinc doses were alleviated by the introduction of compost into the soil. Organic fertilization led to microbial growth intensification and increased biochemical activity of the soil already 2 weeks after compost application. These effects persisted throughout the experiment. Therefore, it can be stated that the use of compost is an appropriate method for restoring normal functions of soil ecosystems contaminated with zinc.
Показать больше [+] Меньше [-]Correction to: Pentachlorophenol Biodegradation by Citrobacter freundii Isolated from Forest Contaminated Soil Полный текст
2017
WerheniAmmeri, Rim | MokniTlili, Sonia | Mehri, Ines | Badi, Souhir | Hassen, Abdennaceur
One of the bibliographic reference entry of this article had the author name and family name switched by mistake.
Показать больше [+] Меньше [-]Production of Surface-Active Compounds by a Hydrocarbon-Degrading Actinobacterium: Presumptive Relationship with Lipase Activity Полный текст
2017
Colin, Verónica Leticia | Bourguignon, Natalia | Gómez, Johana Stefi | de Carvalho, Kátia Gianni | Ferrero, Marcela Alejandra | Amoroso, María Julia
The replacement of synthetic surface-active compounds (SACs) by their microbial counterparts is carving out a niche for themselves in the field of bioremediation. However, the high cost of microbial products has limited their application at a realistic scale. In the current study, several hydrocarbon-degrading microorganisms were assayed as potential SAC producers in low-cost liquid media. Only the strain CC10, placed within the class Actinobacteria, was able to produce emulsifying molecules by using a combination of sugarcane vinasse or crude glycerol (as cheap carbon substrates) with urea or peptone (as nitrogen sources). The emulsifying activity of the supernatants and the stability of emulsions formed with motor oil depended on the carbon and nitrogen sources. However, the biodegradability of these metabolites was only associated with the carbon substrate, and it was always higher than the two tested synthetic surfactants, sodium dodecyl sulfate and Triton X-100. Also, a positive linear association between emulsifying and lipase activities of the CC10 supernatants was detected (r = 0.781; p = 0.219), with the maximum activities detected in the glycerol-peptone supernatant. Interestingly, this supernatant was able to emulsify different oily substrates, a property that could be used to increase the efficiency of the treatment of effluents with high fat content.
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