Bacterial communities inhabiting sediments in coastal areas endure the effect of strong anthropogenic pressure characterized by the presence of multiple contaminants. Understanding the effect of pollutants on the organization of bacterial communities is of paramount importance in order to unravel bacterial assemblages colonizing specific ecological niches. Here, chemical and molecular approaches were combined to investigate the bacterial communities inhabiting the sediments of the Ichkeul Lake/Bizerte Lagoon, a hydrological system under anthropogenic pressure. Although the microbial community of the Ichkeul Lake sediment was different to that of the Bizerte Lagoon, common bacterial genera were identified suggesting a lake-lagoon continuum probably due to the hydrology of the system exchanging waters according to the season. These genera represent bacterial "generalists" maintaining probably general biogeochemical functions. Linear discriminant analysis effect size (LEfSe) showed significant differential abundance distribution of bacterial genera according to the habitat, the pollution type and level. Further, correlation analyses identified specific bacterial genera which abundance was linked with pesticides concentrations in the lake, while in the lagoon the abundance of specific bacterial genera was found linked with the concentrations of PAHs (Polycyclic aromatic hydrocarbons) and organic forms of Sn. As well, bacterial genera which abundance was not correlated with the concentrations of pollutants were identified in both lake and lagoon. These findings represent valuable information, pointing out specific bacterial genera associated with pollutants, which represent assets for developing bacterial tools for the implementation, the management, and monitoring of bioremediation processes to mitigate the effect of pollutants in aquatic ecosystems.

Leachates from municipal landfills are formed as infiltration waters flowing through the landfill. They contain toxic, dissolved products of biochemical reactions taking place in the deposit. They cause soil and groundwater pollution. It is necessary to take them out of the landfill cover and utilize toxins contained therein, in particular heavy metals. Such processes are conducted with the use of microorganisms. Due to the content of toxic compounds, introducing leachates into the process of biological purification poses a threat to the microorganisms used in these processes.An alternative to microbial co-treatment of sludge and leachate as well as soil contaminated with communal leachate is to use red hybrid of California (Eisenia fetida Sav.), an earthworm resistant to environmental toxins, in particular heavy metals.The aim of the conducted research is to demonstrate the possibility of using red hybrid of California in leachate bioutilization as a complementary or alternative method to the process of leachate utilization with the use of microorganisms.The obtained results led to the conclusion that Eisenia fetida accumulates environmental toxins well. By collecting and processing them in the tissues, it remedies the substrate and retains long life and fertility, and the ability to reproduce. The research demonstrated high dynamics of population growth (from 25 individuals in the initial deposit to 298 individuals after six months of research). These properties are related to the presence of enzyme proteins from the metallothionein group in the gastrointestinal tract cells. Packing heavy metals found in leachates into the metallothionein coat limits their toxic effect on earthworm tissues, which confirms the possibility of using earthworms in the processes of detoxification of municipal leachate.

Triphenyl phosphate (TPHP) is one of the major organophosphate esters (OPEs) with increasing consumption. Considering its largely distribution and high toxicity in aquatic environment, it is important to explore an efficient treatment for TPHP. This study aimed to investigate the accelerated degradation of TPHP in a three-electrode single chamber bioelectrochemical system (BES). Significant increase of degradation efficiency of TPHP in the BES was observed compared with open circuit and abiotic controls. The one-order degradation rates of TPHP (1.5 mg L⁻¹) were increased with elevating sodium acetate concentrations and showed the highest value (0.054 ± 0.010 h⁻¹) in 1.0 g L⁻¹ of sodium acetate. This result indicated bacterial metabolism of TPHP was enhanced by the application of micro-electrical field and addition acetate as co-substrates. TPHP could be degraded into diphenyl phosphate (DPHP), hydroxyl triphenyl phosphate (OH-TPHP) and three byproducts. DPHP was the most accumulated degradation product in BES, which accounted more than 35.5% of the initial TPHP. The composition of bacterial community in BES electrode was affected by the acclimation by TPHP, with the most dominant bacteria of Azospirillum, Petrimonas, Pseudomonas and Geobacter at the genera level. Moreover, it was found that the acute toxic effect of TPHP to Vibrio fischeri was largely removed after the treatment, which revealed that BES is a promising technology to remove TPHP threaten in aquatic environment.

Localised sites in Antarctica are contaminated with mixtures of metals, yet the risk this contamination poses to the marine ecosystem is not well characterised. Recent research showed that two Antarctic marine microalgae have antagonistic responses to a mixture of five common metals (Koppel et al., 2018a). However, the metal accumulating potential and risk to secondary consumers through dietary exposure are still unknown. This study investigates cellular accumulation following exposure to a mixture of cadmium, copper, nickel, lead, and zinc for the Antarctic marine microalgae, Phaeocystis antarctica and Cryothecomonas armigera. In both microalgae, cellular cadmium, copper, and lead concentrations increased with increasing exposures while cellular nickel and zinc did not. For both microalgae, copper in the metal mixture drives inhibition of growth rate with R2 values > -0.84 for all cellular fractions in both species and the observed antagonism was likely caused by zinc competition, having significantly positive partial regressions. Metal accumulation to P. antarctica and C. armigera is likely to be toxic to consumer organisms, with low exposure concentrations resulting in cellular concentrations of 500 and 1400 × 10−18 mol Zn cell−1 and 160 and 320 × 10−18 mol Cu cell−1, respectively.

Azoles are effective antifungal agents used in both medicine and agriculture. They typically work by inhibiting cytochrome P450 enzymes, primarily CYP51 of the ergosterol biosynthesis pathway, thus damaging the fungal cell membrane. However, apart from their desired antifungal properties, several azoles also exhibit endocrine disrupting properties in mammals, both in vitro and in vivo. Here, we have tested two currently used agricultural azole fungicides, triticonazole and flusilazole, for their in vitro anti-androgenic activity and potential effects on reproductive parameters. Both fungicides showed strong androgen receptor (AR) antagonism and disruption of steroid biosynthesis in vitro. Following gestational exposure to flusilazole (15 or 45 mg/kg bw/day) or triticonazole (150 or 450 mg/kg bw/day) in time-mated Sprague Dawley rats, triticonazole induced shorter male anogenital distance (AGD). Flusilazole exposure did not affect the AGD, but altered fetal male blood hormone profile, with increased androstenedione and decreased estrone levels. Flusilazole and triticonazole have dissimilar effects on reproductive parameters in vivo, but both show endocrine disrupting activities.

Enhancing metals phytoextraction using gentile mobilizing agents might be an appropriate approach to increase the phytoextraction efficiency and to shorten the phytoremediation duration. The effect of sulfur-impregnated organoclay (SIOC) on the redistribution of potentially toxic elements (PTEs) among their geochemical fractions in soils and their plant uptake has not yet been studied. Therefore, our aim is to investigate the role of different SIOC application doses (1%, 3% and 5%) on operationally defined geochemical fractions (soluble + exchangeable; bound to carbonate; manganese oxide; organic matter; sulfide; poorly- and well-crystalline Fe oxide; and residual fraction) of Cd, Cr, Cu, Ni, Pb, and Zn, and their accumulation by pea (Pisum sativum) and corn (Zea mays) in a greenhouse pot experiment using a polluted floodplain soil. The SIOC caused a significant decrease in soil pH, and an increase in organic carbon and total sulfur content in the soil. The addition of SIOC increased significantly the soluble + exchangeable fraction and bioavailability of the metals. The SIOC leads to a transformation of the residual, organic, and Fe-Mn oxide fractions of Cd, Cu, Ni, and Zn to the soluble + exchangeable fraction. The SIOC addition increased the potential mobile (non-residual) fraction of Cr and Pb. The SIOC increased the sulfide fraction of Cr, Ni, and Zn, while it decreased the same fraction for Cd, Cu, and Pb. The effect of SIOC on the redistribution of metal fractions increased with enhancing application dosages. Pea accumulated more metals than corn with greater accumulation in the roots than shoots. Application of the higher dose of SIOC promoted the metals accumulation by roots and their translocation to shoots of pea and corn. Our results suggest the potential suitability of SIOC for enhancing the phytomanagement of PTEs polluted soils and reducing the environmental risk of these pollutants.

Decabromodiphenyl ethane (DBDPE) is an alternative to the commercial decabromodiphenyl ether (deca-BDE) mixture but has potentially similar persistence, bioaccumulation potential and toxicity. While it is widely used as a flame retardant in electrical and electronic equipment (EEE) in China, DBDPE could be distributed globally on a large scale with the international trade of EEE emanating from China. Here, we performed a dynamic substance flow analysis to estimate the time-dependent mass flows, stocks and emissions of DBDPE in China, and the global spread of DBDPE originating in China through the international trade of EEE and e-waste. Our analysis indicates that, between 2006 and 2016, ∼230 thousand tonnes (kt) of DBDPE were produced in China; production, use and disposal activities led to the release of 196 tonnes of DBDPE to the environment. By the end of 2016, ∼152 kt of the DBDPE produced resided in in-use products across China. During the period 2000–2016, ∼39 kt of DBDPE were exported from China in EEE products, most of which (>50%) ended up in North America. Based on projected trends of China's DBDPE production, use and EEE exports, we predict that, by 2026, ∼74 and ∼14 kt of DBDPE originating in China will reside in in-use and waste stocks, respectively, in regions other than mainland China, which will act as long-term emission sources of DBDPE worldwide. This study discusses the considerable impact of DBDPE originating in China and distributed globally through the international trade of EEE; this is projected to occur on a large scale in the near future, which necessitates countermeasures.

Epidemiological evidence showed that the particulate matter exposure is associated with atherosclerotic plaque progression, which may be related to foam cell formation, but the mechanism is still unknown. The study was aimed to investigate the toxic effects and possible mechanism of PM2.5 on the formation of macrophage foam cells induced by oxidized low density lipoprotein (ox-LDL). Results showed that PM2.5 induced cytotoxicity by decreasing the cell viability and increasing the LDH level in macrophage foam cells. PM2.5 aggravated the lipid accumulation in ox-LDL-stimulated macrophage RAW264.7 within markedly increasing level of intracellular lipid by Oil red O staining. The level of ROS increased obivously after co-exposure to PM2.5 and ox-LDL than single exposure group. In addition, serious mitochondrial damage such as the mitochondrial swelling, cristae rupturing and disappearance were observed in macrophage foam cells. The loss of the mitochondrial membrane potential (MMP) further exacerbated the mitochondrial damage in PM2.5-induced macrophage foam cells. The apoptotic rate increased more severely via up-regulated protein level of Bax, Cyt C, Caspase-9, Caspase-3, and down-regulated that of Bcl-2, indicating that PM2.5 activated the mitochondrial-mediated apoptosis pathway. In summary, our results demonstrated that PM2.5 aggravated the lipid accumulation, mitochondrial damage and apoptosis in macrophage foam cells, suggesting that PM2.5 was a risk factor of atherosclerosis progression.

The aim of the present study was to optimize the protocol for sampling marine macroalgae to be used to biomonitor heavy metal contamination in marine ecosystems. For this purpose, we collected 50 subsamples of the brown seaweed Fucus vesiculosus at random in each of three sampling sites (SS) and determined the concentrations of Al, As, Cd, Co, Cr, Cu, Fe, Hg, N, Ni, Pb, Zn and δ15N. We used semivariograms to explore the possible existence of spatial structure in the concentrations of the elements. Spatial structure was observed in 88% of the semivariograms studied, with element concentrations varying longitudinally and transversally along the SS. Using randomization techniques, we estimated that in each SS, a minimum of 30 evenly distributed subsamples should be collected within three bands parallel to the coastline (and also at different heights on the rocks if necessary), and analyzed in a single composite sample representative of the intra-SS variability.

Synthetic pyrethroids (SPs) are broadly used to control pests and have been widely detected in aquatic environments, prompting concern over its risk to the health of non-target organisms. The present study evaluated whether long-term (60 d) exposure to low doses (0, 20, 100, and 500 ng/L) of cis-BF enantiomers (1S-cis-BF and 1R-cis-BF) could cause reproductive endocrine disturbance to zebrafish. Exposure to 1S-cis-BF has stronger reproductive impairment effect than 1R-enantiomer, indicating that the enantioselectivity of cis-BF on fish reproduction. Significant decrease of cumulative spawning of zebrafish was observed as a result of cis-BF exposure. And the retardations of testis and ovaries development found in histopathological section were suggested to be important cause for the decreased fecundity. Cis-BF decreased the total motility of sperm but did not affect sperm density. Relatively high levels of cis-BF detected in the gonads of males and females may directly impair gametogenesis. In addition, alterations in the expression of key genes (cyp17, cyp19a and 17β-hsd) associated with reproductive endocrine pathways were correlated well with the significant changes in sex hormone contents (E2 and T) and these results may relate to gonadal development and maturation of germ cells in females or/and males which were suspected to be a likely underlying mechanism. Furthermore, the reduction of quality of F1 embryo derived from the unexposed females and exposed males (UE♀ × E♂) demonstrated that male exposure had greater adverse effects on offspring. Our results indicate that long term, low dose exposure to cis-BF can enantioselectively impair the reproduction system of fish, and induce toxicity related abnormalities in non-exposed offspring. This study has important implications for environmental risk assessment of chiral pesticides that are concurrently present in aquatic systems.